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The response of the single-celled ciliates to increased temperature during global warming is critical for the structure and functioning of freshwater food webs. I conducted a meta-analysis of the literature from field studies and experimental evidence to assess the parameters characterising the thermal response of freshwater ciliates. The shape of the thermal performance curve predicts the ciliates’ survival at supraoptimal temperatures (i.e., the width of the thermal safety margin, TSM). The ciliates’ typical TSM is ~5°C. One-third of the freshwater ciliates dwelling permanently or occasionally in the pelagial cannot survive at temperatures exceeding 30°C. Likewise, cold-stenothermic species, which represent a significant fraction of euplanktonic ciliates, cannot survive by evolutionary adaptation to rapidly warming environments. The statistical analysis revealed that the ciliates’ thermal performance is affected by their planktonic lifestyle (euplanktonic versus tychoplanktonic), ability to form cysts, and nutritional ecology. Bactivorous ciliates have the widest temperature niche, and algivorous ciliates have the narrowest temperature niche. Phenotypic plasticity and genetic variation, favouring the selection of pre-adapted species in a new environment, are widespread among freshwater ciliates. However, the lack of evidence for the temperature optima and imprecisely defined tolerance limits of most species hamper the present analysis. The extent of acclimation and adaptation requires further research with more ciliate species than the few chosen thus far. Recent eco-evolutionary experimental work and modelling approaches demonstrated that the ciliates’ thermal responses follow general trends predicted by the metabolic theory of ecology and mechanistic functions inherent in enzyme kinetics. The present analysis identified current knowledge gaps and avenues for future research that may serve as a model study for other biota. Thermal adaptation may conflict with adaptation to other stressors (predators, food availability, pH), making general predictions on the future role of freshwater ciliates in a warmer environment difficult, if not impossible, at the moment. # Data from: Thermal response of freshwater ciliates: can they survive at elevated lake temperatures? [https://doi.org/10.5061/dryad.jdfn2z3jr](https://doi.org/10.5061/dryad.jdfn2z3jr) The dataset results from a meta-analysis to assess the parameters characterising the thermal response of freshwater ciliates (i.e., minimum and maximum temperature tolerated, temperature niche breadth). Cyst formation, the nutritional type, and the planktonic lifestyle were considered as factors affecting the ciliates’ thermal performance. ## Description of the data and file structure The main dataset reporting ciliate species and synonyms, taxonomic affiliation, minimum and maximum temperature and the temperature range tolerated, cysts formation, mixotrophic nutrition, food type, and planktonic lifestyle are reported in the 'Dataset_v4.xlsx' file. This is the main document. Taxonomic affiliation (i.e., order) following Adl et al. (2019, reference [65]J, the GBIF Backbone Taxonomy, and Lynn (2008; reference [66]). Details on the references - i.e., authors, publication year, title, journal/book, volume, and page/article numbers used to compile this dataset and some comments can be found in 'References.xlsx'. Empty cells mean that information is unavailable. References A-E are the main sources of the dataset, i.e., comprehensive review articles published by W. Foissner and colleagues in the 1990s. References 1-64 are case studies, published mainly after 1999. References 65 and 66 refer to the taxonomic affiliation of the ciliate species. More details about each column of the main document can be found in the 'Units_table.xlsx' file. ## Sharing/Access information Data was derived from the following sources: * ISI Web of Science (All Data Bases) * Google Scholar ## Code/Software R statistical software (v 4.0.5, R Core Team 2021) with the packages lme4, lmtest, multcomp, AICcmodavg. WebPlotDigitizer (Version 4.6) for data extraction from figures ## Version changes **06-aug-2024**: Taxonomic affiliation (order) corrected according to GBIF. Genus *Tintinnidium* is now in the order Oligotrichida. I scrutinised the detailed literature compilations by Foissner and colleagues published in the 1990s; these references are listed as primary sources A-E in the Dataset, see References.xlsx and README.txt) to obtain an overview of the thermal performance, resting cyst formation, and nutritional ecology of planktonic freshwater ciliates. I then searched the ISI Web of Science (All Data Bases) for updates and cross-references of Foissner’s works and further temperature records from (mainly) field studies. Search terms (in all fields) for the latter were ciliate* AND temperature NOT marine NOT ocean NOT soil NOT parasit* (1,339 hits). I followed the PRISMA guidelines in combination with EndNote 20 to filter out the records eligible for screening and analysis. Temperature data for assessing the minimum (Tmin) and maximum temperature (Tmax) of occurrence were eventually extracted from 68 publications. However, because Foissner’s works present extensive reviews, the actual number of publications used for the analysis is much higher. The final dataset obtained from field studies comprised 206 ciliate species. Next, I searched the ISI Web of Science for experimental results, using ciliate* AND temperature AND growth rate* NOT marine as search terms (218 records). Removing results from unsuitable research areas (mainly from medical research) reduced the records to 71 publications, which were screened. The combination of ciliate* AND numerical response NOT marine yielded 40 studies, ciliate* AND thermal performance 21 hits. I checked the selected articles for citations and cross-references using Google Scholar to identify any publications that might have slipped my attention. Eventually, I picked experimental results from 18 studies. If the literature data were only shown in figures, I extracted the data from the plots with WebPlotDigitizer (Version 4.6). I analysed the dataset with the R Statistical Software using the packages lme4, lmerTest, stats, multcomp, AICcmodavg and car.

Contrasting physiological mortality with predator-induced mortality is of tremendous importance for the population dynamics of many organisms but is difficult to assess. I performed a meta-analysis using planktonic ciliates as model organisms to estimate the maximum physiological mortality rates (δmax) across pelagic ecosystems in relation to environmental and biotic factors. Data were compiled from published numerical response (NR) experiments and experimentally determined rates of decline (ROD). Variables reported are ciliate species and order, ciliate specific growth rates (rmax), prey species, temperature, habitat (marine vs freshwater), the coefficients of the numerical response experiments, and reported or calculated ciliate mortality rates. The median δmax of planktonic ciliates was 0.62 d−1 and did not differ between marine and freshwater species. Maximum ciliate mortality rates were species-specific and affected by their rmax, cell volume, and ability to encyst. Cyst-forming species had, on average, higher δmax than species unable to encyst. Maximum mortality rates of ciliates were positively related to rmax but appeared unaffected by temperature. I conclude that (i) in the ocean, physiological mortality is more critical for controlling ciliate population size than ciliate losses imposed by microcrustacean predation, but (ii) in many lakes, the opposite holds; (iii) cyst-formation is an effective ciliate trait to cope with the high mortality of motile cells upon starvation. The lack of a temperature effect on δmax deserves further study; if correct, planktonic ciliates may take advantage of rising ocean and lake temperatures, with important implications for the pelagic food web. I used ISI Web of Science and Google Scholar to search for experiments that measured growth and mortality rates of ciliates as a function of prey concentration (i.e. numerical responses). The search terms were “growth (rate)” or “numerical response” in combination with “ciliate*” to search for numerical response experiments and “starvation” or “starved” in combination with “ciliate*” to search for mortality experiments. In addition, I searched the literature cited in these publications for further datasets. I considered only planktonic ciliates. When studies did not report all parameters of the NR curve, the data were extracted from figures with DataThief III or WebPlotDigitizer (Version 4.6) and fitted with a modified Michaelis-Menten equation that included the threshold prey concentration (P’) as an additional parameter. Mortality rates obtained by ROD experiments used the δmax reported in the respective study or calculated δmax from the maximum rate of decline after digitizing the data from the original curves, as described above. The literature search yielded δmax reported from 41 studies investigating 56 species or strains in 81 NR experiments and 19 ROD experiments. The final dataset (n = 77) included 37 studies and 48 species. I analyzed the dataset using the R Statistical Software using the packages lme4, lmerTest, AICcmodavg, and MuMIn. # Physiological mortality rates of planktonic ciliates ## Description of the Data and file structure I used ISI Web of Science and Google Scholar to search for experiments that measured growth and mortality rates of ciliates as a function of prey concentration (i.e. numerical responses). The main dataset containing available experimental studies reporting ciliate species, experimental temperature, prey species, ciliate maximum growth rates, ciliate cell volumes, habitat of ciliate isolation, method of study and reported or calculated ciliate mortality rates are reported in the 'Dataset_v2.xlsx' file. This is the main document. Missing data codes: N.A. = not available; n/a = not applicable. More details about each column of the main document can be found in the 'Units_table.xlsx' file. Details on the references - i.e. authors, publication year, title, journal/book, volume and page/article numbers - used to compile this dataset can be found in 'References.xlsx'. ## Sharing/access Information The individual data were derived mainly from the ISI Web of Science. The data compilation is novel. Excel, R

Replication package of the paper titled "An approach using performance models for supporting energy analysis of software systems". Usage instructions are contained in the README.md file.

In this paper we argue for the need to apply a cognitive approach to understand deep dynamics and determinants of technological evolutions. After examining main contributions from innovation studies to the conceptualization of innovation and change in complex socio-technical environments, we highlight the contribution coming from the application of the cognitive approach to evolutionary studies on technologies and we introduce the concept of technological memory as an interpretative tool to understand those changes. We discuss our hypothesis with reference to several observations carried out in different local contexts – Mexico, India and Italy – in relation to technological change in the water sector. In those cases deliberate attempts to substitute traditional technologies with modern ones led to interesting trajectories of change ranging from the collapse of old technologies to the development of multifaceted hybridization patterns. Tema. Journal of Land Use, Mobility and Environment, 2014: INPUT 2014 - Smart City: planning for energy, transportation and sustainability of the urban system

The INTENSSS PA project, funded by Horizon 2020, the Framework Programme for Research and Innovation of the European Union, aims to support the local authorities involved and their stakeholders to develop an innovative integrated sustainable energy planning concept through a participatory, interdisciplinary and multilevel process. By building individual and institutional capacity of the actors involved, using the Regional Living Lab approach, the concept will be applied in order to develop seven sustainable integrated energy plans. In this first article the project activities and the results achieved so far are preliminary described, anticipating a more extensive and detailed publication on the project planned for the December edition of UPLand – Journal of Urban Planning Landscape & Environmental Design. UPLanD - Journal of Urban Planning, Landscape & environmental Design, GREEN 2.0

Starting from the relationship between urban planning and mobility management, TeMA has gradually expanded the view of the covered topics, always remaining in the groove of rigorous scientific in-depth analysis. This section of the Journal, Review Notes, is the expression of a continuous updating of emerging topics concerning relationships between urban planning, mobility and environment, through a collection of short scientific papers written by young researchers. The Review Notes are made of four parts. Each section examines a specific aspect of the broader information storage within the main interests of TeMA Journal. In particular, the Urban planning literature review section aims at presenting recent books and journals, within global scientific panorama, on selected topics and issues. This contribution aims at delving into the most severe effects due to heat waves and presenting three interesting and significant scientific books and journal that present effective adaptation strategies to limit climate crisis and improve Mediterranean resilience towards more frequent and severe heat waves. The second contribution of the Review Notes for TeMA vo. 15 highlights the need for integrated action to address the climate crisis in the Mediterranean region, bringing together the strengths and weaknesses of its shores, despite social, economic and political differences. Moreover, the extreme weather events that are occurring throughout Europe, from the south to the north, show how the Mediterranean area is particularly sensitive to climate change-related events. TeMA - Journal of Land Use, Mobility and Environment, Vol 15 No 2 (2022): The city challenges and external agents. Methods, tools and best practices

Starting from the relationship between urban planning and mobility management, TeMA has gradually expanded the view of the covered topics, always following a rigorous scientific in-depth analysis. This section of the Journal, Review Notes, is a continuous update about emerging topics concerning relationships among urban planning, mobility and environment thanks to a collection of short scientific papers written by young researchers. The Review Notes are made up of five parts. Each section examines a specific aspect of the broader information storage within the main interests of the TeMA Journal. In particular: The Town Planning International Rules and Legislation Overview section aims at presenting the latest updates in the territorial and urban legislative sphere. The Covid-19 pandemic is causing a major impact on energy systems. Improving the energy efficiency of urban areas is now the cornerstone of scientific community and the European Commission discussions. Thus, the content of this review note aims at carrying out an analysis of the latest documents of the European Commission before and during the slow recovery from Covid-19 with the aim of identifying the priorities and areas on which to intervene to reduce energy consumption in different territorial contexts. TeMA - Journal of Land Use, Mobility and Environment, Vol 14 No 1 (2021): The city challenges and external agents. Methods, tools and best practices

One of the main problems that affects modern cities is connected to transport/mobility. Urban transport is currently based on car use; the transition to the use of more sustainable means of transport is happening slowly. Bicycles used as main way of transport, combined with walking, it’s a successful solution for many towns to really bring traffic and congestion down. For their high density and their short time travels, towns are the best places (in comparison to long time travels as merchandise transport) to reduce the green houses gasses emitted promoting walking, cycling and public transport. For this reason the European Union is directly founding different projects that boost urban cycling. Many examples presented in this paper where collected by an European project. This project sectioned best practices and excellences in cycling as the so called cycle cities: Amsterdam, Copenhagen, Seville,…cities that have recognized the importance of cycling as a solution to traffic congestion. But how is it possible to transfer these experiences to others realities? The scope of this article is to show the sustainability of cycling according to socio-economic (social and economic sustainability) and environmental terms (environmental sustainability). For this reason is proposed a CBA (Cost and Benefits Analysis) methodology specific to evidence the advantages of investments in cycling made by public authorities or private companies both, to promote and realize ecological infrastructures. One of the main problems that affects modern cities is connected to transport/mobility. Urban transport is currently based on car use; the transition to the use of more sustainable means of transport is happening slowly. Bicycles used as main way of transport, combined with walking, it’s a successful solution for many towns to really bring traffic and congestion down. For their high density and their short time travels, towns are the best places (in comparison to long time travels as merchandise transport) to reduce the green houses gasses emitted promoting walking, cycling and public transport. For this reason the European Union is directly founding different projects that boost urban cycling. Many examples presented in this paper where collected by an European project. This project sectioned best practices and excellences in cycling as the so called cycle cities: Amsterdam, Copenhagen, Seville,…cities that have recognized the importance of cycling as a solution to traffic congestion. But how is it possible to transfer these experiences to others realities? The scope of this article is to show the sustainability of cycling according to socio-economic (social and economic sustainability) and environmental terms (environmental sustainability). For this reason is proposed a CBA (Cost and Benefits Analysis) methodology specific to evidence the advantages of investments in cycling made by public authorities or private companies both, to promote and realize ecological infrastructures. Tema. Journal of Land Use, Mobility and Environment, Vol 8, N° 1 (2015): Cities, Energy and Climate Change