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SummaryRecent observations suggest that repeated fires could drive Mediterranean forests to shrublands, hosting flammable vegetation that regrows quickly after fire. This feedback supposedly favours shrubland persistence and may be strengthened in the future by predicted increased aridity. An assessment was made of how fires and aridity in combination modulated the dynamics of Mediterranean ecosystems and whether the feedback could be strong enough to maintain shrubland as an alternative stable state to forest.A model was developed for vegetation dynamics, including stochastic fires and different plant fire‐responses. Parameters were calibrated using observational data from a period up to 100 yr ago, from 77 sites with and without fires in Southeast Spain and Southern France.The forest state was resilient to the separate impact of fires and increased aridity. However, water stress could convert forests into open shrublands by hampering post‐fire recovery, with a possible tipping point at intermediate aridity.Projected increases in aridity may reduce the resilience of Mediterranean forests against fires and drive post‐fire ecosystem dynamics toward open shrubland. The main effect of increased aridity is the limitation of post‐fire recovery. Including plant fire‐responses is thus fundamental when modelling the fate of Mediterranean‐type vegetation under climate‐change scenarios.

An original approach to develop sustainable agriculture with less nitrogen fertilizer inputs is to tackle the cross-talk between nitrogen nutrition and plant growth regulators. In particular the gaseous hormone, ethylene, is a prime target for that purpose. The variation of ethylene production in natural accessions of the model species Arabidopsis thaliana was explored in response to the nitrate supply. Ethylene was measured with a laser-based photoacoustic detector. First, experimental conditions were established with Columbia-0 (Col-0) accession, which was grown in vitro on horizontal plates across a range of five nitrate concentrations (0.5, 1, 2.5, 5, or 10 mM). The concentrations of 1 and 10 mM nitrate were retained for further characterization. Along with a decrease of total dry biomass and higher biomass allocation to the roots, the ethylene production was 50% more important at 1 mM than at 10 mM nitrate. The total transcript levels of 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASES (ACS) in roots and those of ACC OXIDASES (ACO) in shoots increased by 100% between the same treatments. This was mainly due to higher transcript levels of ACS6 and of ACO2 and ACO4 respectively. The assumption was that during nitrogen deficiency, the greater biomass allocation in favor of the roots was controlled by ethylene being released in the shoots after conversion of ACC originating from the roots. Second, biomass and ethylene productions were measured in 20 additional accessions. Across all accessions, the total dry biomass and ethylene production were correlated negatively at 1 mM but positively at 10 mM nitrate. Furthermore, polymorphism was surveyed in ACC and ethylene biosynthesis genes and gene products among accessions. Very few substitutions modifying the amino acids properties in conserved motifs of the enzymes were found in the accessions. Natural variation of ethylene production could be further explored to improve Nitrogen Use Efficiency (NUE), in particular by manipulating features like the biomass production and the timing of senescence upon nitrogen limitation.

Abstract. Accurate quantification of the amount and spatial variation of evapotranspiration is important in a wide range of disciplines. Remote sensing based surface energy balance models have been developed to estimate turbulent surface energy fluxes at different scales. The objective of this study is to evaluate the Surface Energy Balance System (SEBS) model on a landscape scale, using tower-based flux measurements at different land cover units during an overpass of the ASTER sensor over the SPARC 2004 experimental site in Barrax (Spain). A sensitivity analysis has been performed in order to investigate to which variable the sensible heat flux is most sensitive. Taking into account their estimation errors, the aerodynamic parameters (hc, z0M and d0) can cause large deviations in the modelling of sensible heat flux. The effect of replacement of empirical derivation of these aerodynamic parameters in the model by field estimates or literature values is investigated by testing two scenarios: the Empirical Scenario in which empirical equations are used to derive aerodynamic parameters and the Field Scenario in which values from field measurements or literature are used to replace the empirical calculations of the Empirical Scenario. In the case of a homogeneous land cover in the footprints of the measurements, the Field Scenario only resulted in a small improvement, compared to the Empirical Scenario. The Field Scenario can even worsen the result in the case of heterogeneous footprints, by creating sharp borders related to the land cover map. In both scenarios modelled fluxes correspond better with flux measurements over uniform land cover compared to cases where different land covers are mixed in the measurement footprint. Furthermore SEBS underestimates sensible heat flux especially over dry and sparsely vegetated areas, which is common in single-source models.