- NIH| Role of BK Channel Interactome in Excessive Ethanol Drinking ,NIH| Activation of the parasubthalamic nucleus in alcohol dependence ,NIH| CORE--BIOCHEMICAL COREAgbonlahor Okhuarobo; Max Kreifeldt;
Pauravi J. Gandhi; Catherine Lopez; +9 AuthorsPauravi J. Gandhi
Harvested from ORCID Public Data FilePauravi J. Gandhi in OpenAIREAgbonlahor Okhuarobo; Max Kreifeldt; Pauravi J. Gandhi; Catherine Lopez; Briana Martinez;Pauravi J. Gandhi
Harvested from ORCID Public Data FilePauravi J. Gandhi in OpenAIRE Kiera Fleck; Michal Bajo;Kiera Fleck
Harvested from ORCID Public Data FileKiera Fleck in OpenAIRE
Pushpita Bhattacharyya; Alex M. Dopico;Pushpita Bhattacharyya
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesPushpita Bhattacharyya in OpenAIRE Marisa Roberto; Amanda J. Roberts;Marisa Roberto
Harvested from ORCID Public Data FileMarisa Roberto in OpenAIRE Gregg E. Homanics; Gregg E. Homanics
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesGregg E. Homanics in OpenAIRE Candice Contet; Candice Contet
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesCandice Contet in OpenAIREAbstractLarge conductance potassium (BK) channels are among the most sensitive molecular targets of ethanol and genetic variations in the channel-forming α subunit have been nominally associated with alcohol use disorders. However, whether the action of ethanol at BK α influences the motivation to drink alcohol remains to be determined. To address this question, we first tested the effect of systemically administered BK channel modulators on voluntary alcohol consumption in C57BL/6J males. Penitrem A (blocker) exerted dose-dependent effects on moderate alcohol intake, while paxilline (blocker) and BMS-204352 (opener) were ineffective. Because pharmacological manipulations are inherently limited by non-specific effects, we then sought to investigate the behavioral relevance of ethanol’s direct interaction with BK α by introducing in the mouse genome a point mutation known to render BK channels insensitive to ethanol while preserving their physiological function. The BK α K361N substitution prevented ethanol from reducing spike threshold in medial habenula neurons. However, it did not alter acute responses to ethanol in vivo, including ataxia, sedation, hypothermia, analgesia, and conditioned place preference. Furthermore, the mutation did not have reproducible effects on alcohol consumption in limited, continuous, or intermittent access home cage two-bottle choice paradigms conducted in both males and females. Notably, in contrast to previous observations made in mice missing BK channel auxiliary β subunits, the BK α K361N substitution had no significant impact on ethanol intake escalation induced by chronic intermittent alcohol vapor inhalation. It also did not affect the metabolic and locomotor consequences of chronic alcohol exposure. Altogether, these data suggest that the direct interaction of ethanol with BK α does not mediate the alcohol-related phenotypes examined here in mice.
Access RoutesGreen hybrid 2 citations 2 popularity Average influence Average impulse Average 
Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/s41380-023-02346-y&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Role of BK Channel Interactome in Excessive Ethanol Drinking ,NIH| Activation of the parasubthalamic nucleus in alcohol dependence ,NIH| CORE--BIOCHEMICAL COREAgbonlahor Okhuarobo; Max Kreifeldt; Pauravi J. Gandhi; Catherine Lopez; +9 Authors
Pauravi J. Gandhi
Harvested from ORCID Public Data FilePauravi J. Gandhi in OpenAIREAgbonlahor Okhuarobo; Max Kreifeldt; Pauravi J. Gandhi; Catherine Lopez; Briana Martinez;Pauravi J. Gandhi
Harvested from ORCID Public Data FilePauravi J. Gandhi in OpenAIRE Kiera Fleck; Michal Bajo;Kiera Fleck
Harvested from ORCID Public Data FileKiera Fleck in OpenAIRE Pushpita Bhattacharyya; Alex M. Dopico;Pushpita Bhattacharyya
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesPushpita Bhattacharyya in OpenAIRE Marisa Roberto; Amanda J. Roberts;Marisa Roberto
Harvested from ORCID Public Data FileMarisa Roberto in OpenAIRE Gregg E. Homanics; Gregg E. Homanics
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesGregg E. Homanics in OpenAIRE Candice Contet; Candice Contet
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesCandice Contet in OpenAIREAbstractLarge conductance potassium (BK) channels are among the most sensitive molecular targets of ethanol and genetic variations in the channel-forming α subunit have been nominally associated with alcohol use disorders. However, whether the action of ethanol at BK α influences the motivation to drink alcohol remains to be determined. To address this question, we first tested the effect of systemically administered BK channel modulators on voluntary alcohol consumption in C57BL/6J males. Penitrem A (blocker) exerted dose-dependent effects on moderate alcohol intake, while paxilline (blocker) and BMS-204352 (opener) were ineffective. Because pharmacological manipulations are inherently limited by non-specific effects, we then sought to investigate the behavioral relevance of ethanol’s direct interaction with BK α by introducing in the mouse genome a point mutation known to render BK channels insensitive to ethanol while preserving their physiological function. The BK α K361N substitution prevented ethanol from reducing spike threshold in medial habenula neurons. However, it did not alter acute responses to ethanol in vivo, including ataxia, sedation, hypothermia, analgesia, and conditioned place preference. Furthermore, the mutation did not have reproducible effects on alcohol consumption in limited, continuous, or intermittent access home cage two-bottle choice paradigms conducted in both males and females. Notably, in contrast to previous observations made in mice missing BK channel auxiliary β subunits, the BK α K361N substitution had no significant impact on ethanol intake escalation induced by chronic intermittent alcohol vapor inhalation. It also did not affect the metabolic and locomotor consequences of chronic alcohol exposure. Altogether, these data suggest that the direct interaction of ethanol with BK α does not mediate the alcohol-related phenotypes examined here in mice.
Access RoutesGreen hybrid 2 citations 2 popularity Average influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/s41380-023-02346-y&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - EC| NovAnI ,NWO| New concepts in catalytic lignin depolymerization: sustainable pathways towards value added chemicals Anastasiia M. Afanasenko; Xianyuan Wu;
Anastasiia M. Afanasenko
Harvested from ORCID Public Data FileAnastasiia M. Afanasenko in OpenAIRE Alessandra De Santi; Alessandra De Santi
Harvested from ORCID Public Data FileAlessandra De Santi in OpenAIRE Walid A. M. Elgaher; +7 AuthorsWalid A. M. Elgaher
Harvested from ORCID Public Data FileWalid A. M. Elgaher in OpenAIRE Anastasiia M. Afanasenko; Xianyuan Wu;Anastasiia M. Afanasenko
Harvested from ORCID Public Data FileAnastasiia M. Afanasenko in OpenAIRE Alessandra De Santi; Alessandra De Santi
Harvested from ORCID Public Data FileAlessandra De Santi in OpenAIRE Walid A. M. Elgaher; Andreas M. Kany; Roya Shafiei; Marie‐Sophie Schulze; Thomas F. Schulz;Walid A. M. Elgaher
Harvested from ORCID Public Data FileWalid A. M. Elgaher in OpenAIRE Jörg Haupenthal; Jörg Haupenthal
Harvested from ORCID Public Data FileJörg Haupenthal in OpenAIRE Anna K. H. Hirsch; Anna K. H. Hirsch
Harvested from ORCID Public Data FileAnna K. H. Hirsch in OpenAIRE Katalin Barta; Katalin Barta
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesKatalin Barta in OpenAIREAbstractDeriving active pharmaceutical agents from renewable resources is crucial to increasing the economic feasibility of modern biorefineries and promises to alleviate critical supply‐chain dependencies in pharma manufacturing. Our multidisciplinary approach combines research in lignin‐first biorefining, sustainable catalysis, and alternative solvents with bioactivity screening, an in vivo efficacy study, and a structural‐similarity search. The resulting sustainable path to novel anti‐infective, anti‐inflammatory, and anticancer molecules enabled the rapid identification of frontrunners for key therapeutic indications, including an anti‐infective against the priority pathogen Streptococcus pneumoniae with efficacy in vivo and promising plasma and metabolic stability. Our catalytic methods provided straightforward access, inspired by the innate structural features of lignin, to synthetically challenging biologically active molecules with the core structure of dopamine, namely, tetrahydroisoquinolines, quinazolinones, 3‐arylindoles and the natural product tetrahydropapaveroline. Our diverse array of atom‐economic transformations produces only harmless side products and uses benign reaction media, such as tunable deep eutectic solvents for modulating reactivity in challenging cyclization steps.
15 citations 15 popularity Average influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/ange.202308131&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - EC| NovAnI ,NWO| New concepts in catalytic lignin depolymerization: sustainable pathways towards value added chemicals Anastasiia M. Afanasenko; Xianyuan Wu;
Anastasiia M. Afanasenko
Harvested from ORCID Public Data FileAnastasiia M. Afanasenko in OpenAIRE Alessandra De Santi; Alessandra De Santi
Harvested from ORCID Public Data FileAlessandra De Santi in OpenAIRE Walid A. M. Elgaher; +7 AuthorsWalid A. M. Elgaher
Harvested from ORCID Public Data FileWalid A. M. Elgaher in OpenAIRE Anastasiia M. Afanasenko; Xianyuan Wu;Anastasiia M. Afanasenko
Harvested from ORCID Public Data FileAnastasiia M. Afanasenko in OpenAIRE Alessandra De Santi; Alessandra De Santi
Harvested from ORCID Public Data FileAlessandra De Santi in OpenAIRE Walid A. M. Elgaher; Andreas M. Kany; Roya Shafiei; Marie‐Sophie Schulze; Thomas F. Schulz;Walid A. M. Elgaher
Harvested from ORCID Public Data FileWalid A. M. Elgaher in OpenAIRE Jörg Haupenthal; Jörg Haupenthal
Harvested from ORCID Public Data FileJörg Haupenthal in OpenAIRE Anna K. H. Hirsch; Anna K. H. Hirsch
Harvested from ORCID Public Data FileAnna K. H. Hirsch in OpenAIRE Katalin Barta; Katalin Barta
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesKatalin Barta in OpenAIREAbstractDeriving active pharmaceutical agents from renewable resources is crucial to increasing the economic feasibility of modern biorefineries and promises to alleviate critical supply‐chain dependencies in pharma manufacturing. Our multidisciplinary approach combines research in lignin‐first biorefining, sustainable catalysis, and alternative solvents with bioactivity screening, an in vivo efficacy study, and a structural‐similarity search. The resulting sustainable path to novel anti‐infective, anti‐inflammatory, and anticancer molecules enabled the rapid identification of frontrunners for key therapeutic indications, including an anti‐infective against the priority pathogen Streptococcus pneumoniae with efficacy in vivo and promising plasma and metabolic stability. Our catalytic methods provided straightforward access, inspired by the innate structural features of lignin, to synthetically challenging biologically active molecules with the core structure of dopamine, namely, tetrahydroisoquinolines, quinazolinones, 3‐arylindoles and the natural product tetrahydropapaveroline. Our diverse array of atom‐economic transformations produces only harmless side products and uses benign reaction media, such as tunable deep eutectic solvents for modulating reactivity in challenging cyclization steps.
15 citations 15 popularity Average influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/ange.202308131&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Targeting STAT3 in Ovarian Cancer- Center for Integrated Biomedical and Bioengineering (CIBBR) ,NIH| Role of striatal pathways in learning with nicotine stimulusChristopher L. Robison; Victoria Madore; Nicole Cova; Robert C. Drugan; Sergios Charntikov;
Sergios Charntikov
Harvested from ORCID Public Data FileSergios Charntikov in OpenAIREAbstractThis study investigated the relationship between stress exposure and subsequent ethanol use, focusing on individual differences among male rats. We combined operant self-administration with behavioral economics to assess how intermittent swim stress affects ethanol consumption. This approach allowed for a nuanced analysis of the transition from regular ethanol intake to stress-induced escalation in economic demand. Results showed a consistent rise in ethanol demand post-stress among subjects, irrespective of exposure to actual swim stress or a sham procedure. This increase may result from a two-week abstinence or an inherent rise in demand over time. Significantly, we identified a direct link between post-stress corticosterone levels and the demand for ethanol, considering baseline levels. This correlation was particularly pronounced when examining the shifts in both corticosterone levels and demand for ethanol post-stress. However, neither post-stress corticosterone levels nor their change over time correlated significantly with changes in ethanol demand following a forced swim test that was administered 24 hours after the intermittent swim stress test. This suggests potential context-specific or stressor-specific effects. Importantly, pre-stress ethanol demand did not significantly predict the corticosterone response to stress, indicating that high ethanol-demand rats do not inherently exhibit heightened stress sensitivity. Our research brings to light the complex interplay between stress and ethanol consumption, highlighting the critical role of individual differences in this relationship. This research introduces a nuanced perspective, underscoring the need for future studies in the realm of stress and substance use to give greater consideration to individual variability.
Access RoutesGreen gold 1 citations 1 popularity Average influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1101/2024.02.26.582066&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Targeting STAT3 in Ovarian Cancer- Center for Integrated Biomedical and Bioengineering (CIBBR) ,NIH| Role of striatal pathways in learning with nicotine stimulusChristopher L. Robison; Victoria Madore; Nicole Cova; Robert C. Drugan; Sergios Charntikov;
Sergios Charntikov
Harvested from ORCID Public Data FileSergios Charntikov in OpenAIREAbstractThis study investigated the relationship between stress exposure and subsequent ethanol use, focusing on individual differences among male rats. We combined operant self-administration with behavioral economics to assess how intermittent swim stress affects ethanol consumption. This approach allowed for a nuanced analysis of the transition from regular ethanol intake to stress-induced escalation in economic demand. Results showed a consistent rise in ethanol demand post-stress among subjects, irrespective of exposure to actual swim stress or a sham procedure. This increase may result from a two-week abstinence or an inherent rise in demand over time. Significantly, we identified a direct link between post-stress corticosterone levels and the demand for ethanol, considering baseline levels. This correlation was particularly pronounced when examining the shifts in both corticosterone levels and demand for ethanol post-stress. However, neither post-stress corticosterone levels nor their change over time correlated significantly with changes in ethanol demand following a forced swim test that was administered 24 hours after the intermittent swim stress test. This suggests potential context-specific or stressor-specific effects. Importantly, pre-stress ethanol demand did not significantly predict the corticosterone response to stress, indicating that high ethanol-demand rats do not inherently exhibit heightened stress sensitivity. Our research brings to light the complex interplay between stress and ethanol consumption, highlighting the critical role of individual differences in this relationship. This research introduces a nuanced perspective, underscoring the need for future studies in the realm of stress and substance use to give greater consideration to individual variability.
Access RoutesGreen gold 1 citations 1 popularity Average influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1101/2024.02.26.582066&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - EC| Smartphon ,EC| REWIRE ,EC| BORGES ,EC| PLASMMONS Vasileiadis, Thomas; Marchesi D’Alvise, Tommaso;
Vasileiadis, Thomas
Harvested from ORCID Public Data FileVasileiadis, Thomas in OpenAIRE Saak, Clara-Magdalena; Saak, Clara-Magdalena
Harvested from ORCID Public Data FileSaak, Clara-Magdalena in OpenAIRE Pochylski, Mikolaj; +7 AuthorsPochylski, Mikolaj
Harvested from ORCID Public Data FilePochylski, Mikolaj in OpenAIRE Vasileiadis, Thomas; Marchesi D’Alvise, Tommaso;Vasileiadis, Thomas
Harvested from ORCID Public Data FileVasileiadis, Thomas in OpenAIRE Saak, Clara-Magdalena; Saak, Clara-Magdalena
Harvested from ORCID Public Data FileSaak, Clara-Magdalena in OpenAIRE Pochylski, Mikolaj; Harvey, Sean;Pochylski, Mikolaj
Harvested from ORCID Public Data FilePochylski, Mikolaj in OpenAIRE Synatschke, Christopher V.; Synatschke, Christopher V.
Harvested from ORCID Public Data FileSynatschke, Christopher V. in OpenAIRE Gapinski, Jacek; Gapinski, Jacek
Harvested from ORCID Public Data FileGapinski, Jacek in OpenAIRE Fytas, George; Fytas, George
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesFytas, George in OpenAIRE Backus, Ellen H. G.; Backus, Ellen H. G.
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesBackus, Ellen H. G. in OpenAIRE Weil, Tanja; Weil, Tanja
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesWeil, Tanja in OpenAIRE Graczykowski, Bartlomiej; Graczykowski, Bartlomiej
Harvested from ORCID Public Data FileGraczykowski, Bartlomiej in OpenAIREThe actuation of micro- and nanostructures controlled by external stimuli remains one of the exciting challenges in nanotechnology due to the wealth of fundamental questions and potential applications in energy harvesting, robotics, sensing, biomedicine, and tunable metamaterials. Photoactuation utilizes the conversion of light into motion through reversible chemical and physical processes and enables remote and spatiotemporal control of the actuation. Here, we report a fast light-to-motion conversion in few-nanometer thick bare polydopamine (PDA) membranes stimulated by visible light. Light-induced heating of PDA leads to desorption of water molecules and contraction of membranes in less than 140 μs. Switching off the light leads to a spontaneous expansion in less than 20 ms due to heat dissipation and water adsorption. Our findings demonstrate that pristine PDA membranes are multiresponsive materials that can be harnessed as robust building blocks for soft, micro-, and nanoscale actuators stimulated by light, temperature, and moisture level.
Access RoutesGreen hybrid add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1021/acs.nanolett.1c03165&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - EC| Smartphon ,EC| REWIRE ,EC| BORGES ,EC| PLASMMONS Vasileiadis, Thomas; Marchesi D’Alvise, Tommaso;
Vasileiadis, Thomas
Harvested from ORCID Public Data FileVasileiadis, Thomas in OpenAIRE Saak, Clara-Magdalena; Saak, Clara-Magdalena
Harvested from ORCID Public Data FileSaak, Clara-Magdalena in OpenAIRE Pochylski, Mikolaj; +7 AuthorsPochylski, Mikolaj
Harvested from ORCID Public Data FilePochylski, Mikolaj in OpenAIRE Vasileiadis, Thomas; Marchesi D’Alvise, Tommaso;Vasileiadis, Thomas
Harvested from ORCID Public Data FileVasileiadis, Thomas in OpenAIRE Saak, Clara-Magdalena; Saak, Clara-Magdalena
Harvested from ORCID Public Data FileSaak, Clara-Magdalena in OpenAIRE Pochylski, Mikolaj; Harvey, Sean;Pochylski, Mikolaj
Harvested from ORCID Public Data FilePochylski, Mikolaj in OpenAIRE Synatschke, Christopher V.; Synatschke, Christopher V.
Harvested from ORCID Public Data FileSynatschke, Christopher V. in OpenAIRE Gapinski, Jacek; Gapinski, Jacek
Harvested from ORCID Public Data FileGapinski, Jacek in OpenAIRE Fytas, George; Fytas, George
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesFytas, George in OpenAIRE Backus, Ellen H. G.; Backus, Ellen H. G.
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesBackus, Ellen H. G. in OpenAIRE Weil, Tanja; Weil, Tanja
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesWeil, Tanja in OpenAIRE Graczykowski, Bartlomiej; Graczykowski, Bartlomiej
Harvested from ORCID Public Data FileGraczykowski, Bartlomiej in OpenAIREThe actuation of micro- and nanostructures controlled by external stimuli remains one of the exciting challenges in nanotechnology due to the wealth of fundamental questions and potential applications in energy harvesting, robotics, sensing, biomedicine, and tunable metamaterials. Photoactuation utilizes the conversion of light into motion through reversible chemical and physical processes and enables remote and spatiotemporal control of the actuation. Here, we report a fast light-to-motion conversion in few-nanometer thick bare polydopamine (PDA) membranes stimulated by visible light. Light-induced heating of PDA leads to desorption of water molecules and contraction of membranes in less than 140 μs. Switching off the light leads to a spontaneous expansion in less than 20 ms due to heat dissipation and water adsorption. Our findings demonstrate that pristine PDA membranes are multiresponsive materials that can be harnessed as robust building blocks for soft, micro-, and nanoscale actuators stimulated by light, temperature, and moisture level.
Access RoutesGreen hybrid add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1021/acs.nanolett.1c03165&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Sheketha R. Hauser; Patrick J. Mulholland; William A. Truitt;
Patrick J. Mulholland
Harvested from ORCID Public Data FilePatrick J. Mulholland in OpenAIRE R. Aaron Waeiss; +3 AuthorsR. Aaron Waeiss
Harvested from ORCID Public Data FileR. Aaron Waeiss in OpenAIRESheketha R. Hauser; Patrick J. Mulholland; William A. Truitt;Patrick J. Mulholland
Harvested from ORCID Public Data FilePatrick J. Mulholland in OpenAIRE R. Aaron Waeiss; Eric A. Engleman;R. Aaron Waeiss
Harvested from ORCID Public Data FileR. Aaron Waeiss in OpenAIRE Richard L. Bell; Richard L. Bell
Harvested from ORCID Public Data FileRichard L. Bell in OpenAIRE Zachary A. Rodd; Zachary A. Rodd
Harvested from ORCID Public Data FileZachary A. Rodd in OpenAIREA consistent preclinical finding is that exposure to alcohol during adolescence produces a persistent hyperdopaminergic state during adulthood. The current experiments determine that effects of Adolescent Intermittent Ethanol (AIE) on the adult neurochemical response to EtOH administered directly into the mesolimbic dopamine system, alterations in dendritic spine and gene expression within the nucleus accumbens shell (AcbSh), and if treatment with the HDACII inhibitor TSA could normalize the consequences of AIE. Rats were exposed to the AIE (4 g/kg ig; 3 days a week) or water (CON) during adolescence, and all testing occurred during adulthood. CON and AIE rats were microinjected with EtOH directly into the posterior VTA and dopamine and glutamate levels were recorded in the AcbSh. Separate groups of AIE and CON rats were sacrificed during adulthood and Taqman arrays and dendritic spine morphology assessments were performed. The data indicated that exposure to AIE resulted in a significant leftward and upward shift in the dose-response curve for an increase in dopamine in the AcbSh following EtOH microinjection into the posterior VTA. Taqman array indicated that AIE exposure affected the expression of target genes (Chrna7, Impact, Chrna5). The data indicated no alterations in dendritic spine morphology in the AcbSh or any alteration in AIE effects by TSA administration. Binge-like EtOH exposure during adolescence enhances the response to acute ethanol challenge in adulthood, demonstrating that AIE produces a hyperdopaminergic mesolimbic system in both male and female Wistar rats. The neuroadaptations induced by AIE in the AcbSh could be part of the biological basis of the observed negative consequences of adolescent binge-like alcohol exposure on adult drug self-administration behaviors.
Access RoutesGreen gold 8 citations 8 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/ijms222111733&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Sheketha R. Hauser; Patrick J. Mulholland; William A. Truitt;
Patrick J. Mulholland
Harvested from ORCID Public Data FilePatrick J. Mulholland in OpenAIRE R. Aaron Waeiss; +3 AuthorsR. Aaron Waeiss
Harvested from ORCID Public Data FileR. Aaron Waeiss in OpenAIRESheketha R. Hauser; Patrick J. Mulholland; William A. Truitt;Patrick J. Mulholland
Harvested from ORCID Public Data FilePatrick J. Mulholland in OpenAIRE R. Aaron Waeiss; Eric A. Engleman;R. Aaron Waeiss
Harvested from ORCID Public Data FileR. Aaron Waeiss in OpenAIRE Richard L. Bell; Richard L. Bell
Harvested from ORCID Public Data FileRichard L. Bell in OpenAIRE Zachary A. Rodd; Zachary A. Rodd
Harvested from ORCID Public Data FileZachary A. Rodd in OpenAIREA consistent preclinical finding is that exposure to alcohol during adolescence produces a persistent hyperdopaminergic state during adulthood. The current experiments determine that effects of Adolescent Intermittent Ethanol (AIE) on the adult neurochemical response to EtOH administered directly into the mesolimbic dopamine system, alterations in dendritic spine and gene expression within the nucleus accumbens shell (AcbSh), and if treatment with the HDACII inhibitor TSA could normalize the consequences of AIE. Rats were exposed to the AIE (4 g/kg ig; 3 days a week) or water (CON) during adolescence, and all testing occurred during adulthood. CON and AIE rats were microinjected with EtOH directly into the posterior VTA and dopamine and glutamate levels were recorded in the AcbSh. Separate groups of AIE and CON rats were sacrificed during adulthood and Taqman arrays and dendritic spine morphology assessments were performed. The data indicated that exposure to AIE resulted in a significant leftward and upward shift in the dose-response curve for an increase in dopamine in the AcbSh following EtOH microinjection into the posterior VTA. Taqman array indicated that AIE exposure affected the expression of target genes (Chrna7, Impact, Chrna5). The data indicated no alterations in dendritic spine morphology in the AcbSh or any alteration in AIE effects by TSA administration. Binge-like EtOH exposure during adolescence enhances the response to acute ethanol challenge in adulthood, demonstrating that AIE produces a hyperdopaminergic mesolimbic system in both male and female Wistar rats. The neuroadaptations induced by AIE in the AcbSh could be part of the biological basis of the observed negative consequences of adolescent binge-like alcohol exposure on adult drug self-administration behaviors.
Access RoutesGreen gold 8 citations 8 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/ijms222111733&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Ventral Prefrontal Network Connectivity and Alcohol Sensitivity in Bipolar Disorder and Typically Developing Young Adults ,NIH| Genetic Mechanisms of Change in Trajectories of Drinking and Deviant Behaviors ,NIH| Dissecting Responses to Alcohol in Individuals with Familial Risk for Bipolar Disorder ,NIH| Subjective Response to Alcohol and Associated Neural Systems in Bipolar Disorder Dylan E. Kirsch; Raquel Kosted; Vanessa Le;
Dylan E. Kirsch
Harvested from ORCID Public Data FileDylan E. Kirsch in OpenAIRE Jorge R. C. Almeida; +3 AuthorsJorge R. C. Almeida
Harvested from ORCID Public Data FileJorge R. C. Almeida in OpenAIRE Dylan E. Kirsch; Raquel Kosted; Vanessa Le;Dylan E. Kirsch
Harvested from ORCID Public Data FileDylan E. Kirsch in OpenAIRE Jorge R. C. Almeida; Kim Fromme; Stephen M. Strakowski;Jorge R. C. Almeida
Harvested from ORCID Public Data FileJorge R. C. Almeida in OpenAIRE Elizabeth T. C. Lippard; Elizabeth T. C. Lippard
Harvested from ORCID Public Data FileElizabeth T. C. Lippard in OpenAIREBipolar disorder co-occurs with alcohol use disorder at a rate 3-5 times higher than the general population. We recently reported that individuals with bipolar disorder differ in the positive stimulating and anxiolytic effects of alcohol compared with healthy peers. This study used a randomized, placebo-controlled, cross-over, within-subject alcohol administration design to investigate neurobiological mechanisms within ventral prefrontal cortical (vPFC) systems that may underlie altered sensitivity to alcohol in bipolar disorder (NCT04063384). Forty-seven young adults (n = 23 with bipolar disorder, 64% women) completed clinical assessment and two beverage administration sessions (alcohol and placebo, counter-balanced). Participants were dosed to 0.08 g% breath alcohol concentration during the alcohol condition and completed measures of subjective response to alcohol and an emotional processing fMRI task during the ascending limb. Timing during the placebo condition mirrored the alcohol session. Acute alcohol was associated with reduced functional connectivity between the insula - subcallosal cingulate cortex, and increased connectivity between the left nucleus accumbens - ventromedial PFC in bipolar disorder, but with no change in functional connectivity between these regions in healthy peers. Alcohol-related increases in nucleus accumbens - ventromedial PFC functional connectivity was associated with greater positive stimulating effects of alcohol in bipolar disorder and heavier recent alcohol use. Results suggest vPFC brain systems respond differently to acute alcohol during emotional processing in young adults with bipolar disorder compared with healthy peers, and that vPFC system responses relate to the subjective experience of intoxication and recent alcohol use.
3 citations 3 popularity Average influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/s41386-023-01657-6&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Ventral Prefrontal Network Connectivity and Alcohol Sensitivity in Bipolar Disorder and Typically Developing Young Adults ,NIH| Genetic Mechanisms of Change in Trajectories of Drinking and Deviant Behaviors ,NIH| Dissecting Responses to Alcohol in Individuals with Familial Risk for Bipolar Disorder ,NIH| Subjective Response to Alcohol and Associated Neural Systems in Bipolar Disorder Dylan E. Kirsch; Raquel Kosted; Vanessa Le;
Dylan E. Kirsch
Harvested from ORCID Public Data FileDylan E. Kirsch in OpenAIRE Jorge R. C. Almeida; +3 AuthorsJorge R. C. Almeida
Harvested from ORCID Public Data FileJorge R. C. Almeida in OpenAIRE Dylan E. Kirsch; Raquel Kosted; Vanessa Le;Dylan E. Kirsch
Harvested from ORCID Public Data FileDylan E. Kirsch in OpenAIRE Jorge R. C. Almeida; Kim Fromme; Stephen M. Strakowski;Jorge R. C. Almeida
Harvested from ORCID Public Data FileJorge R. C. Almeida in OpenAIRE Elizabeth T. C. Lippard; Elizabeth T. C. Lippard
Harvested from ORCID Public Data FileElizabeth T. C. Lippard in OpenAIREBipolar disorder co-occurs with alcohol use disorder at a rate 3-5 times higher than the general population. We recently reported that individuals with bipolar disorder differ in the positive stimulating and anxiolytic effects of alcohol compared with healthy peers. This study used a randomized, placebo-controlled, cross-over, within-subject alcohol administration design to investigate neurobiological mechanisms within ventral prefrontal cortical (vPFC) systems that may underlie altered sensitivity to alcohol in bipolar disorder (NCT04063384). Forty-seven young adults (n = 23 with bipolar disorder, 64% women) completed clinical assessment and two beverage administration sessions (alcohol and placebo, counter-balanced). Participants were dosed to 0.08 g% breath alcohol concentration during the alcohol condition and completed measures of subjective response to alcohol and an emotional processing fMRI task during the ascending limb. Timing during the placebo condition mirrored the alcohol session. Acute alcohol was associated with reduced functional connectivity between the insula - subcallosal cingulate cortex, and increased connectivity between the left nucleus accumbens - ventromedial PFC in bipolar disorder, but with no change in functional connectivity between these regions in healthy peers. Alcohol-related increases in nucleus accumbens - ventromedial PFC functional connectivity was associated with greater positive stimulating effects of alcohol in bipolar disorder and heavier recent alcohol use. Results suggest vPFC brain systems respond differently to acute alcohol during emotional processing in young adults with bipolar disorder compared with healthy peers, and that vPFC system responses relate to the subjective experience of intoxication and recent alcohol use.
3 citations 3 popularity Average influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/s41386-023-01657-6&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Daniel T. Sangiamo; Michael J. Weingarten; Nnamdi G. Nelson; Chan Young Choi; Aditi Das;
Aditi Das
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesAditi Das in OpenAIRE Nu-Chu Liang; Nu-Chu Liang
Harvested from ORCID Public Data FileNu-Chu Liang in OpenAIRECombined use of cannabis and alcohol is common in adolescents. However, the extent to which such polydrug exposure affects the brain and behaviors remains under-investigated in preclinical studies. This study tested the hypothesis that combined exposure of Δ-9-tetrahydrocannabinol (THC), the main psychoactive constituent of cannabis, and alcohol will have additive effects on cognitive impairments and altered endocannabinoid levels in the hippocampus and frontal cortex. Male Long Evans rats were provided with daily access to cookies laced with oil or dronabinol, a synthetic THC, during adolescence. Three days after discontinuation of edible THC, the effect of orally administered 3 g/kg alcohol on Barnes maze performance was assessed. The results showed that experience with edible THC facilitated the occurrence of increased moving speed on the maze induced by repeated alcohol administration. However, contrasting to the hypothesis, the combined THC and alcohol exposure did not lead to additive deficits in learning and memory on the Barnes maze. While little effect on endocannabinoid levels was observed in the hippocampus, acute abstinence from alcohol significantly reduced endocannabinoid levels in the frontal cortex. In particular, reduction of N-oleoyl ethanolamine (OEA) and N-stearoyl ethanolamine (SEA) were robust and had an interactive effect with discontinuation from edible THC. These findings add to the scarce literature on THC and alcohol associated changes in endocannabinoid levels and provide insights to future investigations on the roles of OEA and SEA on physiology and behaviors following THC and alcohol co-exposure during adolescence.
2 citations 2 popularity Top 10% influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.bbr.2023.114587&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Daniel T. Sangiamo; Michael J. Weingarten; Nnamdi G. Nelson; Chan Young Choi; Aditi Das;
Aditi Das
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesAditi Das in OpenAIRE Nu-Chu Liang; Nu-Chu Liang
Harvested from ORCID Public Data FileNu-Chu Liang in OpenAIRECombined use of cannabis and alcohol is common in adolescents. However, the extent to which such polydrug exposure affects the brain and behaviors remains under-investigated in preclinical studies. This study tested the hypothesis that combined exposure of Δ-9-tetrahydrocannabinol (THC), the main psychoactive constituent of cannabis, and alcohol will have additive effects on cognitive impairments and altered endocannabinoid levels in the hippocampus and frontal cortex. Male Long Evans rats were provided with daily access to cookies laced with oil or dronabinol, a synthetic THC, during adolescence. Three days after discontinuation of edible THC, the effect of orally administered 3 g/kg alcohol on Barnes maze performance was assessed. The results showed that experience with edible THC facilitated the occurrence of increased moving speed on the maze induced by repeated alcohol administration. However, contrasting to the hypothesis, the combined THC and alcohol exposure did not lead to additive deficits in learning and memory on the Barnes maze. While little effect on endocannabinoid levels was observed in the hippocampus, acute abstinence from alcohol significantly reduced endocannabinoid levels in the frontal cortex. In particular, reduction of N-oleoyl ethanolamine (OEA) and N-stearoyl ethanolamine (SEA) were robust and had an interactive effect with discontinuation from edible THC. These findings add to the scarce literature on THC and alcohol associated changes in endocannabinoid levels and provide insights to future investigations on the roles of OEA and SEA on physiology and behaviors following THC and alcohol co-exposure during adolescence.
2 citations 2 popularity Top 10% influence Average impulse Average Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.bbr.2023.114587&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| HMGB1 and innate immune involvement in adult neuropathology following adolescent alcohol exposure ,NIH| Molecular and Cellular Pathogenesis in Alcoholism ,NIH| 5/8 NADIA U01 Adolescent Alcohol and Adult Brian Dysfunction ,NIH| Supplement to Molecular and Cellular Studies on Alcohol's Actions ,NIH| Adolescent Alcohol in 5xFAD Mouse Model Accelerates Neuroinflammation and Alzheimer's Disease Pathology Across Aging ,NIH| 1/1 NADIA U24 Administrative Core Victoria Macht;
Victoria Macht
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesVictoria Macht in OpenAIRE Ryan Vetreno; Natalie Elchert; Fulton Crews;Ryan Vetreno
Harvested from ORCID Public Data FileRyan Vetreno in OpenAIREAbstractBackgroundBinge ethanol exposure during adolescence reduces hippocampal neurogenesis, a reduction which persists throughout adulthood despite abstinence. This loss of neurogenesis, indicated by reduced doublecortin+ immunoreactivity (DCX+IR), is paralleled by an increase in hippocampal proinflammatory signaling cascades. As galantamine, a cholinesterase inhibitor, has anti-inflammatory actions, we tested the hypothesis that galantamine would prevent (study 1) or restore (study 2) AIE induction of proinflammatory signals within the hippocampus as well as AIE-induced loss of hippocampal neurogenesis.MethodsGalantamine (4 mg/kg) or vehicle (saline) was administered to Wistar rats during adolescent intermittent ethanol (AIE; 5.0 g/kg ethanol, 2 days on/2 days off, postnatal day [P] 25-54) (study 1, prevention) or after AIE during abstinent maturation to adulthood (study 2, restoration).ResultsResults indicate AIE reduced DCX+IR and induced cleaved caspase3 (Casp3) in DCX-expressing immature neurons. Excitingly, AIE induction of activated Casp3 in DCX-expressing neurons is both prevented and reversed by galantamine treatment, which also resulted in prevention and restoration of neurogenesis (DCX+IR). Similarly, galantamine prevented and/or reversed AIE induction of proinflammatory markers, including the chemokine (C-C motif) ligand 2 (CCL2), cyclooxygenase-2 (COX-2), and high mobility group box 1 (HMGB1) protein, suggesting that AIE induction of proinflammatory signaling mediates both cell death cascades and hippocampal neurogenesis. Interestingly, galantamine treatment increased Ki67+IR generally as well as increased pan-Trk expression specifically in AIE-treated rats but failed to reverse AIE induction of NADPH-oxidase (gp91phox).ConclusionsCollectively, our studies suggest that (1) loss of neurogenesis after AIE is mediated by persistent induction of proinflammatory cascades which drive activation of cell death machinery in immature neurons, and (2) galantamine can prevent and restore AIE disruptions in the hippocampal environmental milieu to then prevent and restore AIE-mediated loss of neurogenesis.
Access RoutesGreen gold 14 citations 14 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s12974-021-02243-7&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| HMGB1 and innate immune involvement in adult neuropathology following adolescent alcohol exposure ,NIH| Molecular and Cellular Pathogenesis in Alcoholism ,NIH| 5/8 NADIA U01 Adolescent Alcohol and Adult Brian Dysfunction ,NIH| Supplement to Molecular and Cellular Studies on Alcohol's Actions ,NIH| Adolescent Alcohol in 5xFAD Mouse Model Accelerates Neuroinflammation and Alzheimer's Disease Pathology Across Aging ,NIH| 1/1 NADIA U24 Administrative Core Victoria Macht;
Victoria Macht
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesVictoria Macht in OpenAIRE Ryan Vetreno; Natalie Elchert; Fulton Crews;Ryan Vetreno
Harvested from ORCID Public Data FileRyan Vetreno in OpenAIREAbstractBackgroundBinge ethanol exposure during adolescence reduces hippocampal neurogenesis, a reduction which persists throughout adulthood despite abstinence. This loss of neurogenesis, indicated by reduced doublecortin+ immunoreactivity (DCX+IR), is paralleled by an increase in hippocampal proinflammatory signaling cascades. As galantamine, a cholinesterase inhibitor, has anti-inflammatory actions, we tested the hypothesis that galantamine would prevent (study 1) or restore (study 2) AIE induction of proinflammatory signals within the hippocampus as well as AIE-induced loss of hippocampal neurogenesis.MethodsGalantamine (4 mg/kg) or vehicle (saline) was administered to Wistar rats during adolescent intermittent ethanol (AIE; 5.0 g/kg ethanol, 2 days on/2 days off, postnatal day [P] 25-54) (study 1, prevention) or after AIE during abstinent maturation to adulthood (study 2, restoration).ResultsResults indicate AIE reduced DCX+IR and induced cleaved caspase3 (Casp3) in DCX-expressing immature neurons. Excitingly, AIE induction of activated Casp3 in DCX-expressing neurons is both prevented and reversed by galantamine treatment, which also resulted in prevention and restoration of neurogenesis (DCX+IR). Similarly, galantamine prevented and/or reversed AIE induction of proinflammatory markers, including the chemokine (C-C motif) ligand 2 (CCL2), cyclooxygenase-2 (COX-2), and high mobility group box 1 (HMGB1) protein, suggesting that AIE induction of proinflammatory signaling mediates both cell death cascades and hippocampal neurogenesis. Interestingly, galantamine treatment increased Ki67+IR generally as well as increased pan-Trk expression specifically in AIE-treated rats but failed to reverse AIE induction of NADPH-oxidase (gp91phox).ConclusionsCollectively, our studies suggest that (1) loss of neurogenesis after AIE is mediated by persistent induction of proinflammatory cascades which drive activation of cell death machinery in immature neurons, and (2) galantamine can prevent and restore AIE disruptions in the hippocampal environmental milieu to then prevent and restore AIE-mediated loss of neurogenesis.
Access RoutesGreen gold 14 citations 14 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s12974-021-02243-7&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Indiana Clinical and Translational Sciences Institute Richard M. van Rijn;
Richard M. van Rijn
Harvested from ORCID Public Data FileRichard M. van Rijn in OpenAIRE Zhong Ding; Gregory T. Knipp; Julia A. Chester; +1 AuthorsZhong Ding
Harvested from ORCID Public Data FileZhong Ding in OpenAIRE Richard M. van Rijn; Richard M. van Rijn
Harvested from ORCID Public Data FileRichard M. van Rijn in OpenAIRE Zhong Ding; Gregory T. Knipp; Julia A. Chester; Val J. Watts;Zhong Ding
Harvested from ORCID Public Data FileZhong Ding in OpenAIREHeterologous sensitization of adenylyl cyclase (AC) is defined by an enhanced cAMP response following persistent activation of Gαi/o-coupled receptors. This phenomenon was first observed in cellular models, and later reported in animal models of inflammatory pain or following chronic exposure to drugs of abuse including opioids and cocaine. Recently, we used genome-wide siRNA screening to identify Cullin3 signaling as a mediator of AC sensitization in cellular models. We also showed that pharmacological inhibition of Cullin3 with the neddylation inhibitor, MLN4924, abolished heterologous sensitization of several AC isoforms, including AC1, AC2, AC5, and AC6. Because ACs, especially AC1, have been implicated in alcohol-induced locomotor sensitization and inflammatory pain, we assessed the potential activity of MLN4924 in both murine models. We found that MLN4924 (30 mg/kg, i.p.) accumulated in the brain and reduced both locomotor sensitization induced by repeated alcohol administration and allodynia in an inflammatory pain model. Based on our previous findings that MLN4924 potently blocks AC sensitization in cellular models, we propose that the activity of MLN4924 in both animal models potentially occurs through blocking AC sensitization. Our findings provide the basis for understanding the molecular mechanism and yield a new pathway for drug development for pathological disorders associated with AC sensitization.
Access Routesbronze 8 citations 8 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.bbr.2020.113051&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - NIH| Indiana Clinical and Translational Sciences Institute Richard M. van Rijn;
Richard M. van Rijn
Harvested from ORCID Public Data FileRichard M. van Rijn in OpenAIRE Zhong Ding; Gregory T. Knipp; Julia A. Chester; +1 AuthorsZhong Ding
Harvested from ORCID Public Data FileZhong Ding in OpenAIRE Richard M. van Rijn; Richard M. van Rijn
Harvested from ORCID Public Data FileRichard M. van Rijn in OpenAIRE Zhong Ding; Gregory T. Knipp; Julia A. Chester; Val J. Watts;Zhong Ding
Harvested from ORCID Public Data FileZhong Ding in OpenAIREHeterologous sensitization of adenylyl cyclase (AC) is defined by an enhanced cAMP response following persistent activation of Gαi/o-coupled receptors. This phenomenon was first observed in cellular models, and later reported in animal models of inflammatory pain or following chronic exposure to drugs of abuse including opioids and cocaine. Recently, we used genome-wide siRNA screening to identify Cullin3 signaling as a mediator of AC sensitization in cellular models. We also showed that pharmacological inhibition of Cullin3 with the neddylation inhibitor, MLN4924, abolished heterologous sensitization of several AC isoforms, including AC1, AC2, AC5, and AC6. Because ACs, especially AC1, have been implicated in alcohol-induced locomotor sensitization and inflammatory pain, we assessed the potential activity of MLN4924 in both murine models. We found that MLN4924 (30 mg/kg, i.p.) accumulated in the brain and reduced both locomotor sensitization induced by repeated alcohol administration and allodynia in an inflammatory pain model. Based on our previous findings that MLN4924 potently blocks AC sensitization in cellular models, we propose that the activity of MLN4924 in both animal models potentially occurs through blocking AC sensitization. Our findings provide the basis for understanding the molecular mechanism and yield a new pathway for drug development for pathological disorders associated with AC sensitization.
Access Routesbronze 8 citations 8 popularity Top 10% influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.bbr.2020.113051&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Hannah S. Rennebaum; Daniel L. Brummerloh; Stefan Benders;
Stefan Benders
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesStefan Benders in OpenAIRE Alexander Penn; Alexander Penn
Harvested from ORCID Public Data FileAlexander Penn in OpenAIREUnderstanding and predicting the hydrodynamics of gas bubbles and particle-laden phase in fluidized beds is essential for the successful design and efficient operation of this type of reactor. In this work, we used real-time magnetic resonance imaging (MRI) to investigate the effect of baffles on gas bubble behavior and particle motion in a fluidized bed model with an inner diameter of 190 mm. MRI time series of the local particle density and velocity were acquired and used to study the size, number, and shape of gas bubbles as well as the motion of the particle phase. The superficial gas velocity was varied between 1 and 2 Umf . We found that baffles decreased the average equivalent bubble diameter with a simultaneous increase in the total number of bubbles. Moreover, baffles promoted bubble splitting and the formation of air cushions below the baffles and decreased the average particle velocity and acceleration in the bed. For two of the three investigated baffle types, the particle velocity distribution became wider compared to the bed without internal.
6 citations 6 popularity Average influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.powtec.2023.119114&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu - Hannah S. Rennebaum; Daniel L. Brummerloh; Stefan Benders;
Stefan Benders
Derived by OpenAIRE algorithms or harvested from 3rd party repositoriesStefan Benders in OpenAIRE Alexander Penn; Alexander Penn
Harvested from ORCID Public Data FileAlexander Penn in OpenAIREUnderstanding and predicting the hydrodynamics of gas bubbles and particle-laden phase in fluidized beds is essential for the successful design and efficient operation of this type of reactor. In this work, we used real-time magnetic resonance imaging (MRI) to investigate the effect of baffles on gas bubble behavior and particle motion in a fluidized bed model with an inner diameter of 190 mm. MRI time series of the local particle density and velocity were acquired and used to study the size, number, and shape of gas bubbles as well as the motion of the particle phase. The superficial gas velocity was varied between 1 and 2 Umf . We found that baffles decreased the average equivalent bubble diameter with a simultaneous increase in the total number of bubbles. Moreover, baffles promoted bubble splitting and the formation of air cushions below the baffles and decreased the average particle velocity and acceleration in the bed. For two of the three investigated baffle types, the particle velocity distribution became wider compared to the bed without internal.
6 citations 6 popularity Average influence Average impulse Top 10% Powered by BIP!add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.<script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.powtec.2023.119114&type=result"></script>'); --> </script>For further information contact us at helpdesk@openaire.eu
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