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* Technical Challenges - Technology is relatively simple and has high conversion efficiency. * Cost Challenges - Despite efficiency, levelised costs are high, due to mainly 2 factors (1) the input cost of raw material is high, and (2) operating costs are high due to feedstock (methanol) and distillation operations. Selling oilcake has a significant effect on final product cost, with a 50% oilcake internal subsidy reducing the costs by R 6,500/ t (0.65 R/kWh). This would bring production cost into line with current range of diesel prices. * Environmental Challenges - Greenhouse gas savings are significant provided land use changes are carbon neutral. Limiting cultivation to subsistence cropland should assist with this goal. * Social and Institutional Challenges - Conversion of subsistence farmers in former homeland areas, with high reliance on cattle and maize, to a cash crop with side products for own consumption and cattle feed will require significant community involvement. Cooperative farming and marketing channels need to be investigated.

Dataset containing meiobenthos data for samples collected during the September 2008 Sesame Cruise in the North-West Black Sea on board of the Romanian R/V Mare Nigrum. Meiobenthos samples were collected in 5 stations, using a multicorer MARK II-400. The dataset includes 5 samples analysed for meiobenthos species composition, abundance and biomass.The entire washed sample was analyzed under the binocular stereomicroscope. Meiobenthic species were identified and enumerated; some meiobenthic species were identified and enumerated only at higher taxonomic level. Taxonomic identification was done at GEOECOMAR.

A subset of the Field Crop Boundaries data set, showing all subsistence farmland used for crop cultivation. Prepared by SAEON from data provided by DAFF.

Data on cropland was obtained from the global data set produced by the UN Food and Agriculture Organisation (FAO). Data set was obtained as a raster image, and clipped to the boundaries of South Africa, before being converted to a vector layer. The BioEnergy Atlas bases its analyses on mesozones (Planning zones of approximately 50 km2, with relatively homogeneous attributes). This data set aggregates FAO Cropland to mesozones for planning purposes. The FGGD land cover occurrence maps are global raster data layers with a resolution of 5 arc-minutes. Each pixel in each map contains a value representing the percentage of the area belonging to the land

ATP3 Unified Field Study DataThe Algae Testbed Public-Private Partnership ATP3 was established with the goal of investigating open pond algae cultivation across different geographic climatic seasonal and operational conditions while setting the benchmark for quality data collection analysis and dissemination. Identical algae cultivation systems and data analysis methodologies were established at testbed sites across the continental United States and Hawaii. Within this framework the Unified Field Studies UFS were designed to characterize the cultivation of different algal strains during all 4 seasons across this testbed network. The dataset presented here is the complete curated climatic cultivation harvest and biomass composition data for each season at each site. These data enable others to do in-depth cultivation harvest techno-economic life cycle resource and predictive growth modeling analysis as well as develop crop protection strategies for the nascent algae industry.NREL Sub award Number DE-AC36-08-GO28308

Brazil is home to the largest tracts of tropical vegetation in the world, harbouring high levels of biodiversity and carbon. Several biomass maps have been produced for Brazil, using different approaches and methods, and for different purposes. These maps have been used to estimate historic, recent, and future carbon emissions from land use change (LUC). It can be difficult to determine which map to use for what purpose. The implications of using an unsuitable map can be significant, since the maps have large differences—both in terms of total carbon storage and its spatial distribution. This dataset of aboveground carbon was created based on data from existing maps and an up-to-date LULC map. The map reflects current LULC, has high accuracy and resolution (50 m), and a national coverage. It can be a useful alternative for scientific studies and policy initiatives concerned with existing LULC and LUC outside of existing forests, especially at local scales when high resolution is necessary, and/or outside the Amazon biome. Map unit: tonnes of aboveground carbon per hectare. This dataset of aboveground carbon was created based on data from existing maps and an up-to-date LULC map. The map reflects current LULC, has high accuracy and resolution (50 m), and a national coverage. It can be a useful alternative for scientific studies and policy initiatives concerned with existing LULC and LUC outside of existing forests, especially at local scales when high resolution is necessary, and/or outside the Amazon biome. Map unit: tonnes of aboveground carbon per hectare. Data är baserade på befintliga kartor och en aktuell LULC-karta (änding av markanvändning) för bildandet av ovanjordiskt kol i Brasilien. Kartan speglar nuvarande LULC, har hög noggrannhet och upplösning (50 m) och en nationell täckning. Mer information på den engelska katalogsidan: https://snd.gu.se/en/catalogue/study/ecds0244 This dataset of aboveground carbon was created based on data from existing maps and an up-to-date LULC map. The map reflects current LULC, has high accuracy and resolution (50 m), and a national coverage. It can be a useful alternative for scientific studies and policy initiatives concerned with existing LULC and LUC outside of existing forests, especially at local scales when high resolution is necessary, and/or outside the Amazon biome. Map unit: tonnes of aboveground carbon per hectare.

The samples were concentrated down to 50 cm**3 by slow decantation after storage for 20 days in a cool and dark place. The species identification was done under light microscope OLIMPUS–BS41 connected to a video-interactive image analysis system at magnification of the ocular 10X and objective – 40X. A Sedgwick-Rafter camera (1ml) was used for counting. 400 specimen were counted for each sample, while rare and large species were checked in the whole sample (Manual of phytoplankton, 2005). Species identification was mainly after Carmelo T. (1997) and Fukuyo, Y. (2000).Taxon-specific phytoplankton abundance and biomass were analysed by Moncheva S., B. Parr, 2005. Manual for Phytoplankton Sampling and Analysis in the Black Sea.The cell biovolume was determined based on morpho-metric measurement of phytoplankton units and the corresponding geometric shapes as described in detail in (Edier, 1979).

An extensive compilation of field data on net primary productivity (NPP) of natural and agricultural ecosystems worldwide was synthesized in the 1970s and early 1980s by Prof. H. Lieth, Dr. G. Esser and others. Much of this work was carried out at the University of Osnabrueck, Germany. More than 700 single point estimates of NPP or biomass were extracted from the scientific literature, each with a geographical reference (latitude/longitude). The literature cited dates from 1869 to 1982, with the majority of references from the 1960s and 1970s. Although this data set has not been updated since the 1980s, it represents a wealth of information for use in model development and validation. In the early 1970s, a subset of these NPP data was used by Lieth, Esser and co-workers to develop and test a series of statistical-correlative models of NPP as a function of mean annual temperature and precipitation. The later versions of these models included modifications for soil, seasonality, agriculture, and other human influences ("Osnabrück Biosphere Mode,""High Resolution Biosphere Model," etc.). Most of the 720 unique NPP records (632, or 88 percent) have been matched to a bibliography of 356 references from the primary literature. The original form of this bibliography contained many more references than records, including multiple sources for the same author and study, as well as additional references to data on standing biomass, soils, and so forth. Since this is a useful resource in its own right, an edited and corrected compilation of these 858 references is available here with the cross-references to the NPP records highlighted. Of the 720 unique NPP records, about two-thirds have above-ground NPP estimates that range between 1 and 8530 g/m2/year (dry matter) -- or 2923 g/m2/year, excluding doubtful values, wetlands, and crops/pastures and other likely managed systems. Total NPP, for which more than half of the sites have estimates, ranges from 3 to 9320 g/m2/year (dry matter) -- or 3580 g/m2/year, excluding doubtful values, wetlands, and crops/pastures and other likely managed systems. Each record includes a site identifier, latitude, longitude, author, country, NPP estimates, vegetation type, and other variables. The vegetation-type field begins with a generalized biome type (including tundra, forest, Mediterranean, savanna, grassland, desert, wetland, and a number of managed vegetation types) and is followed by more specific vegetation terminology derived from the original data. Caution is advised in using these biome/vegetation types because they were not defined consistently within the original data set and nearly 200 sites lack any vegetation designation. To achieve completeness in a single synthesis file, a single NPP value (NPP_C) is included for each site that represents the sum of above-ground (ANPP) and below-ground (BNPP) components, expressed in grams of carbon per square meter per year (g C/m2/year). Where BNPP was not reported, it was assumed to be equal to ANPP. A ratio of 0.475 was used to convert dry biomass weight to carbon content. Total NPP was estimated as TNPP (where available), or as the sum of ANPP and BNPP (or from ANPP x 2, if BNPP was not estimated), and then converted to g C/m2/year.

Nets are towed obliquely at approx. 1 knot, from the surface to approx. 175 m. Towing time is approx. 20 minutes. Zooplankton (weak swimmers >200µm) are collected using oblique tows of a 1 m**2 net (3m length) with 202µm mesh Nitex netting.

Patterns of movement of marine species can reflect strategies of reproduction and dispersal, species’ interactions, trophodynamics, and susceptibility to change, and thus critically inform how we manage populations and ecosystems. On coral reefs, the density and diversity of metazoan taxa is greatest in dead coral and rubble, which is suggested to fuel food webs from the bottom-up. Yet, biomass and secondary productivity in rubble is predominantly available in some of the smallest individuals, limiting how accessible this energy is to higher trophic levels. We address the bioavailability of motile coral reef cryptofauna based on small-scale patterns of emigration in rubble. We deployed modified RUbble Biodiversity Samplers (RUBS) and emergence traps in a shallow rubble patch at Heron Island, Great Barrier Reef, to detect community-level differences in the directional influx of motile cryptofauna under five habitat accessibility regimes. The mean density (0.13–4.5 ind.cm-3) and biomass (0.14–5.2 mg.cm-3) of cryptofauna were high and varied depending on microhabitat accessibility. Emergent zooplankton represented a distinct community (dominated by the Appendicularia and Calanoida) with the lowest density and biomass, indicating constraints on nocturnal resource availability. Mean cryptofauna density and biomass were greatest when interstitial access within rubble was blocked, driven by the rapid proliferation of small harpacticoid copepods from the rubble surface, leading to trophic simplification. Individuals with high biomass (e.g., decapods, gobies, and echinoderms) were greatest when interstitial access within rubble was unrestricted. Treatments with a closed rubble surface did not differ from those completely open, suggesting that top-down predation does not diminish rubble-derived resources. Our results show that conspecific cues and species’ interactions (e.g., competition and predation) within rubble are most critical in shaping ecological outcomes within the cryptobiome. These findings have implications for prey accessibility through trophic and community size structuring in rubble, which may become increasingly relevant as benthic reef complexity shifts in the Anthropocene. We address the bioavailability of coral reef cryptofauna in rubble based on small-scale patterns of emigration. We adapted the accessibility of Rubble Biodiversity Samplers (RUBS), models used to standardise biodiversity sampling in rubble (Wolfe and Mumby 2020), to explore the local movement patterns of rubble-dwelling fauna, with inference to predation processes within and beyond the cryptobenthos. Five treatments were developed to detect community-level differences in the directional influx of motile cryptofauna under various habitat accessibility regimes. Four of these treatments were developed by modifying accessibility into RUBS (https://www.thingiverse.com/thing:4176644/files) to understand limitations on the directional influx and movement of cryptofauna within coral rubble patches using four treatments; (1) open (completely accessible), (2) interstitial access (top closed), (3) surficial access (sides and bottom closed), and (4) raised (above rubble substratum). The fifth treatment involved a series of emergence plankton traps, designed to target demersal cryptofauna that vertically migrate from within the rubble benthos at night, given emergent zooplankton biomass and diversity are greatest at night. Fieldwork was conducted over several weeks (11th September to 5th October 2021) in a shallow (~3–5 m depth) reef slope site on the southern margin of Heron Island (-23˚26.845’ S, 151˚54.732’ E), Great Barrier Reef, Australia (Fig. 1). All collections were conducted under the Great Barrier Reef Marine Park Authority permit G20/44613.1.