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Forest soil acidification caused by acid deposition is a serious threat to the forest ecosystem. To investigate the liming effects of biomass ash (BA) and alkaline slag (AS) on the acidic topsoil and subsoil, a three-year field experiment under artificial Masson pine was conducted at Langxi, Anhui province in Southern China. The surface application of BA and AS significantly increased the soil pH, and thus decreased exchangeable acidity and active Al in the topsoil. Soil exchangeable Ca2+ and Mg2+ in topsoil were significantly increased by the surface application of BA and AS, while an increase in soil exchangeable K+ was only observed in BA treatments. The soil acidity and active Al in subsoil were decreased by the surface application of AS. Compared with the control, soluble monomeric and exchangeable Al in the subsoil was decreased by 38.0% and 29.4% after 3 years of AS surface application. There was a minimal effect on soluble monomeric and exchangeable Al after the application of BA. The soil exchangeable Ca2+ and Mg2+ in the subsoil increased respectively by 54% and 141% after surface application of 10 t ha-1 AS. The decrease of soil active Al and increase of base cations in subsoil were mainly attributed to the high migration capacity of base cations in AS. In conclusion, the effect of surface application of AS was superior to BA in ameliorating soil acidity and alleviating soil Al toxicity in the subsoil of this Ultisol.

Comparison of the water budget for the typical cropland and pear orchard ecosystems in the North China Plain Comparison of the water budget for the typical cropland and pear orchard ecosystems in the North China Plain

A pot experiment was undertaken to investigate the effects of Cd and Cu mixtures to growth and nutrients (sugar, carotene or vitamin C) of carrot and pakchoi under greenhouse cultivation condition. The study included: (a) physical-chemical properties of soil and soil animals in response to Cd and Cu stress; (b) bioaccumulation of heavy metals, length, biomass, contents of sugar and carotene (vitamin C) of carrot and pakchoi; (c) estimation the effects of Cd and Cu mixtures by multivariate regression analysis. The results implied that heavy metals impacted negative influence on soil animals' abundance. The metals contents in plants increased obviously with Cd and Cu contamination in soil. The biomass production and nutrients declined with Cd and Cu contents increasing. Cd (20 mg kg-1) treatment caused maximum reduction of sugar content (45.29%) in carrot root; maximum reduction in carotene content (75.73%) in carrot, 75.1% sugar content reduction and 70.58% vitamin C content reduction in pakchoi shoots were observed with addition of Cd (20 mg kg-1) and Cu (400 mg kg-1) mixture. The results of multivariate regression analysis indicated that combination of Cd and Cu exerts negative effects to both carrot and pakchoi, and both growth and nutrients were negatively correlated with metals concentrations. It is concluded that the Cd and Cu mixtures caused toxic damage to vegetable plants as Cd and Cu gradient concentrations increased.

Two mesophilic anaerobic bacterial strains (Z7T and Z1) were isolated from waste water sludge of the Xinanzhang paper mill, Beijing, China. The strains were Gram-positive, non-spore-forming and motile. Cells were thin rods (0·2–0·4×4·0–8·0 μm). Growth of the strains was observed at 20–42 °C and pH 5·0–7·5. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. The fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids isoC15 : 0 (42·83 %) and isoC14 : 0 (32·11 %). The DNA G+C contents of strains Z7T and Z1 were 50·4 and 48·6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a new phyletic sublineage within the Clostridium leptum rRNA cluster, with <91 % 16S rRNA gene sequence similarity to currently described species. On the basis of polyphasic evidence from this study, Acetanaerobacterium elongatum gen. nov., sp. nov., a novel genus and species, is proposed, with strain Z7T (=JCM 12359T=AS 1.5012T) as the type strain.

Influence of different pretreated sludge for electricity generation in microbial fuel cells (MFCs) was investigated in this study. Pre-treatment has shown significant improvement in MFC electricity productivity especially from microwave treated sludge. Higher COD reduction in the MFC has been revealed from microwave treated sludge with 55% for total and 85% for soluble COD, respectively. Nonetheless, longer ozonation treatment did not give additional advantage compared to the raw sludge. On the other hand, samples from anodes were analyzed using the 16S rRNA gene-based pyrosequencing technique for microbial community analysis. There was substantial difference in community compositions among MFCs fed with different pretreated sludge. Bacteroidetes was the abundant bacterial phylum dominated in anodes of higher productivity MFCs. These results demonstrate that using waste sludge as the substrate in MFCs could achieve both sludge reduction and electricity generation, and proper pre-treatment of sludge could improve the overall process performance.

The Nanwenghe Wetlands Reserve in the Yile'huli Mountains is a representative region of the Xing'an permafrost. The response of permafrost to climate change remains unclear due to limited field investigations. Thus, longer-term responses of the ground thermal state to climate change since 2011 have been monitored at four sites with varied surface characteristics: Carex tato wetland (P1) and shrub-C. tato wetland (P2) with a multi-year average temperatures at the depth of zero annual amplitude (TZAA) of −0.52 and −1.19 °C, respectively; Betula platyphylla-Larix gmelinii (Rupr.) Kuzen mixed forest (P3) with TZAA of 0.17 °C, and; the forest of L. gmelinii (Rupr.) Kuzen (P4) with TZAA of 1.65 °C. Continuous observations demonstrate that the ecosystem-protected Xing'an permafrost experienced a cooling under a warming climate. The temperature at the top of permafrost (TTOP) rose (1.8 °C per decade) but the TZAA declined (−0.14 °C per decade), while the active layer thickness (ALT) thinned from 0.9 m in 2012 to 0.8 m in 2014 at P1. Both the TTOP and TZAA increased (0.89 and 0.06 °C per decade, respectively), but the ALT thinned from 1.4 m in 2012 to 0.7 m in 2016 at P2. Vertically detached permafrost at P3 disappeared in summer 2012, with warming rates of +0.42 and + 0.17 °C per decade for TTOP and TZAA, respectively. However, up to date, the ground thermal state has remained stable at P4. We conclude that the thermal offset is crucial for the preservation and persistence of the Xing'an permafrost at the southern fringe.

The Upper Yangtze River Basin comprises a densely-populated agricultural region with mountainous and hilly landforms. Intensive cultivation has been extended onto steep hillslopes, which constitute the principal source area for sediment production. Soil conservation on sloping arable lands is thus of utmost priority for persisting sustainable agricultural production and maintaining sound ecosystem services. Although there have been many soil conservation techniques, either promoted by the government or adopted by local farmers, the practiced area was very limited relative to the total area affected by soil erosion. This paper attempts to introduce four popular soil conservation measures on sloping arable lands in this region to enhance a broader scale of implementation, including hedgerow buffers, level trenches, sloping terraces and limited downslope tillage. These practices, although developed from local farmers’ indigenous knowledge for productive purposes, have well conformed to our contemporary understanding of soil erosion processes on sloping landscape affected by human disturbances, were of sound suitability to regional manual tillage agriculture and more trade-off-efficient on rill prevention, runoff harvest and nutrient management.

Climate change-induced glacial melting is a global phenomenon. The effects of climate change-induced melting on the microbial ecology in different glacial-fed aquatic systems have been well illuminated, but the resolution of seasonal dynamics was still limited. Here, we studied bacterial community composition and diversity in a glacial-fed Tibetan lake, Lake Ranwu, to elucidate how glacial-fed aquatic ecosystems respond to the seasonal glacial melting. Obvious seasonal variations of bacterial dominant groups were found in Lake Ranwu and inlet rivers. In April, the majority of OTUs belonged to the Bacteroidetes, Actinobacteria and Proteobacteria. The Proteobacteria increased to the most abundant phylum in July and November, while the Bacteroidetes and Actinobacteria decreased about 50% over seasons. Most key discriminant taxa of each season's community strongly associated with specific environmental variables, suggesting their adaptation to seasonal environments. Bacterial alpha diversity varied among seasons and exhibited strongly negative correlations with conductivity. Conductivity was the major driving force in determining the seasonal variation of bacterial community composition. Fluctuated conductivity was one of the consequences of seasonal melting of glaciers. This study offered evidence for the unique seasonal dynamics pattern of bacterial communities responding to glacial melting. Moreover, this study may provide a reference for assessing the long-term effects of glacial retreat on glacial-fed aquatic ecosystems.