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KOF Working Papers, 327

Historical exploitation of the Mediterranean Sea and the absence of rigorous baselines makes it difficult to evaluate the current health of the marine ecosystems and the efficacy of conservation actions at the ecosystem level. Here we establish the first current baseline and gradient of ecosystem structure of nearshore rocky reefs at the Mediterranean scale. We conducted underwater surveys in 14 marine protected areas and 18 open access sites across the Mediterranean, and across a 31-fold range of fish biomass (from 3.8 to 118 g m(-2)). Our data showed remarkable variation in the structure of rocky reef ecosystems. Multivariate analysis showed three alternative community states: (1) large fish biomass and reefs dominated by non-canopy algae, (2) lower fish biomass but abundant native algal canopies and suspension feeders, and (3) low fish biomass and extensive barrens, with areas covered by turf algae. Our results suggest that the healthiest shallow rocky reef ecosystems in the Mediterranean have both large fish and algal biomass. Protection level and primary production were the only variables significantly correlated to community biomass structure. Fish biomass was significantly larger in well-enforced no-take marine reserves, but there were no significant differences between multi-use marine protected areas (which allow some fishing) and open access areas at the regional scale. The gradients reported here represent a trajectory of degradation that can be used to assess the health of any similar habitat in the Mediterranean, and to evaluate the efficacy of marine protected areas.

A transition from fossil to renewable energy requires the development of sustainable electric energy storage systems capable to accommodate an increasing amount of energy, at larger power and for a longer time. Flow batteries are seen as one promising technology to face this challenge. As different innovations in this field of technology are still under development, reproducible, comparable and verifiable life cycle assessment studies are crucial to providing clear evidence on the sustainability of different flow battery systems. Based on a review of 20 relevant life cycle assessment studies for different flow battery systems, published between 1999 and 2021, this contribution explored relevant methodological choices regarding the sequence of phases defined in the ISO 14,040 series: goal and scope definition, inventory analysis, impact assessment and interpretation. Inspired by good practice examples, common gaps and weaknesses were identified and recommendations for comparative life cycle assessment studies were derived. This includes suggestions for an expanded functional unit definition, a provision of more detailed and transparent reporting of LCI data while using input/output tables. Outcomes of this study are also of relevance for the amendment of the Batteries Directive 2006/66/EC, where first drafts are under revision in the European Council, including the introduction of a battery passport, which should encourage battery producers to reduce their carbon footprint and avoid problematic materials.

Large-scale muscle injury in humans initiates a complex regeneration process, as not only the muscular, but also the vascular and neuro-muscular compartments have to be repaired. Conventional therapeutic strategies often fall short of reaching the desired functional outcome, due to the inherent complexity of natural skeletal muscle. Tissue engineering offers a promising alternative treatment strategy, aiming to achieve an engineered tissue close to natural tissue composition and function, able to induce long-term, functional regeneration after in vivo implantation. This review aims to summarize the latest approaches of tissue engineering skeletal muscle, with specific attention toward fabrication, neuro-angiogenesis, multicellularity and the biochemical cues that adjuvate the regeneration process.

<p>Historical weather data represent an extremely precious resource for agro-meteorology for studying evolutionary dynamics and for predictive purposes, to address agronomical and management choices, that have economic, social and environmental effect. The study of climatic variability and its consequences starts from the observation of variations over time and the identification of the causes, on the basis of historical series of meteorological observations. The availability of long-lasting, complete and accurate datasets is a fundamental requirement to predict and react to climate variability. Inter-annual climate changes deeply affect grapevine productive cycle determining direct impact on the onset and duration of phenological stages and, ultimately, on the grape harvest and yield. Indeed, climate variables, such as air temperature and precipitation, affect evapotranspiration rates, plant water requirements, and also the vine physiology. In this respect, the observed increase in the number of warm days poses a threat to grape quality as it creates a situation of imbalance at maturity, with respect to sugar content, acidity and phenolic and aromatic ripeness.</p><p>A study was conducted to investigate the relationships between climate variables and harvest onset dates to assess the responses of grapevine under a global warming scenario. The study was carried out in the “Monferrato” area, a rainfed hillslope vine-growing area of NW Italy. In particular, the onset dates of harvest of different local wine grape varieties grown in the Vezzolano Experimental Farm (CNR-IMAMOTER) and in surrounding vineyards (affiliated to the Terre dei Santi Cellars) were recorded from 1962 to 2019 and then related to historical series of climate data by means of regression analysis. The linear regression was performed based on the averages of maximum and minimum daily temperatures and sum of precipitation (1962–2019) calculated for growing and ripening season, together with a bioclimatic heat index for vineyards, the Huglin index. The climate data were obtained from two data series collected in the Experimental farm by a mechanical weather station (1962-2002) and a second series recorded (2002-2019) by an electro-mechanical station included in Piedmont Regional Agro-meteorological Network. Finally, a third long-term continuous series covering the period from 1962 to 2019, provided by Italian Meteorological Society was considered in the analysis.</p><p>The results of the study highlighted that inter-annual climate variability, with a general positive trend of temperature, significantly affects the ripening of grapes with a progressive anticipation of the harvest onset dates. In particular, all the considered variables excepted precipitation, resulted negatively correlated with the harvest onset date reaching a high level of significance (up to P< 0.001). Best results have been obtained for maximum temperature and Huglin index, especially by using the most complete dataset. The change ratios obtained using datasets including last 15 years were greater (in absolute terms) than results limited to the period 1962-2002, and also correlations have greater level of significance. The results indicated clearly the relationships between the temperature trend and the gradual anticipation of harvest and the importance of having long and continuous historical weather data series available.</p>

The research focus has shifted towards lightweight structures with high energy absorption capabilities due to advancements in automotive safety technology. This study specifically investigates the impact of cross-sectional area on the energy absorption characteristics of hemispherical composite shells. The experimental phase involves characterizing a glass fiber epoxy composite, followed by the manufacture of hemispherical composite shell specimens with varying cross-sectional areas. These specimens undergo quasi-static axial compressive loading, and the energy absorption parameters are analyzed. The results indicate a significant influence of the composite cross-sectional area on the crushing behavior of hemispherical shells, with a observed decrease in specific energy absorption as the cross-sectional area increases. Additionally, a 3D Finite Element (FE) model is created using ABAQUS FE code to numerically simulate the crushing process. The model's predictions are compared and validated against experimentally measured values, demonstrating a satisfactory correlation.