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This work has been developed under the AGROinLOG Project, “Demonstration of innovative integrated biomass logistics centres for the Agro-industry sector in Europe”. An Integrated Biomass Logistics Center (IBLC), is based on the introduction of new production chains into existing agro-industries by using new biomass feedstock. The AGROinLOG Project has dedicated great attention to investigate the potential of cereal chaff as a valuable resource.Chaff is the fine fraction of the thrashing residues, not usually collected. Chaff is made up of glumes, seed husks, rachis and the tinner part of the cereal stems, whole and cracked kernels, as well as weed seeds.Currently there are several mechanical solutions available on the market for chaff recovery, and others are still at prototype stage, but theyare not so common and very often unknown to the farmers.So far, the literature reportsfew cases of chaff collection with the specific purpose of weed seeds removal, but it still lacks specificexperiments on these machinesintentionally used for biomass collection.For this reason, during the Project AGROinLOG a series of large field tests were performed using an independent scientific approach with different kind of chaff harvesting technologiesin France, Sweden and Italy from 2017 to 2019.The present study collects the results of these activities with the aim to fill that gap and provide deeper understanding in the possibility to enhance the current cereal harvesting method, in order to improve the quantity of biomass collected by including the chaff. Proceedings of the 29th European Biomass Conference and Exhibition, 26-29 April 2021, Online, pp. 62-68

Increasing agricultural productivity is one of the most important aims of modern biotechnology. One way to enhance the productivity of crop species is to enhance the efficiency of photosynthesis. In C3 plants the oxygenase activity of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) limits the photosynthetic efficiency. The green microalgae Chlamydomonas reinhardtii has evolved a CO2 concentration mechanism (CCM) by increasing the CO2 concentration in the chloroplast stroma where RuBisCO is located. Four independent transgenic tobacco genotypes (LA, LB, C1 and C3) were generated producing the low CO2-inducible protein A and B (LCIA and LCIB) or the carbonic anhydrases I and III (CAH1 and CAH3) from C. reinhardtii CCM in the envelope, stroma, intermembrane space or thylakoid lumen of tobacco chloroplasts, respectively. All four recombinant proteins were active in planta, which had a substantial impact on carbon and nitrogen metabolism. Increasing the CO2 concentration near RuBisCO resulted in an enhanced rate of photosynthesis (by up to 15%), efficiency of photosystem II (by up to 18%) and chlorophyll content (by up to 19%). Although to differing extents, all four transgenic genotypes grew faster than wild-type plants, produced more shoot biomass (up to 45% more fresh weight or 38% more dry weight in the LA lines) and accumulated more photosynthetic end products, reflecting the higher rate of photosynthetic CO2 fixation. The proteome analysis revealed that the proteins changed in the transgenic genotypes compared to the wild-type plants were primarily associated with the regulation of the Calvin cycle and the amino acid biosynthesis. Metabolic analysis of the transgenic LA, LB and C3 plants revealed an increase in the levels of carbohydrates and also of most amino acids. Furthermore, transgenic LA and LB plants could maintain the enhanced biomass under low nitrogen conditions, where similarly-treated wild-type plants grew more slowly. The data generated in the present study confirmed that even single Chlamydomonas CCM components can be integrated into C3 plants to increase biomass, suggesting that transgenic lines combining multiple components or even a complete CCM could further increase the productivity and yield of C3 crops. RWTH Aachen University, Diss., 2017; Aachen, 1 Online-Ressource (151 Seiten) : Illustrationen, Diagramme(2017). = RWTH Aachen University, Diss., 2017 Published by Aachen

Ressourcen stellen die Basis für eine erfolgreiche industrielle und technologische Entwicklung dar und somit auch für den Wohlstand heutiger und zukünftiger Generationen. Mit steigender Ressourcennutzung nehmen auch die (physische und sozio-ökonomische) Verfügbarkeit abiotischer und biotischer Ressourcen, die Umweltverschmutzung und die sozialen Auswirkungen durch den Abbau und Nutzung der Ressourcen zu. Um den Erfolg implementierter Strategien (und deren Maßnahmen) hinsichtlich ihres Beitrags zu einem effizienten und nachhaltigen Umgang mit Ressourcen zu bewerten, bedarf es an entsprechenden Bewertungsmethoden. Diese Dissertation stellt vier Methoden bereit um die Bewertung abiotischer und biotischer Ressourcennutzung im Kontext der Nachhaltigkeit auf Produkt- und regionaler Ebene in konsistenter Weise zu bewerten. Die Methode zur Bewertung abiotischer Ressourcen auf Produktebene betrachtet insgesamt 21 relevante Aspekte und stellt Indikatoren zur Quantifizierung bereit. Für die Bewertung der sozio-ökonomischen Einschränkungen von Lieferketten ist eine neuer Ansatz entwickelt, der geopolitische, politische und regulative Aspekte berücksichtigt. Des Weiteren sind Screening-Indikatoren verfügbar, die die gesellschaftliche Akzeptanz der Ressourcennutzung adressieren. Um die Verfügbarkeit terrestrischer biotischer Ressourcen in Produktsystemen zu bewerten, wurde eine umfassende Methode mit 25 Indikatoren erstellt. Des Weiteren wird ein Ansatz vorgestellt, der es ermöglicht eine konsistente Zusammenführung und somit auch Bewertung verschiedener Ressourcentypen zu ermöglichen. Er findet bei der Zusammenführung der entwickelten Methoden zur Bewertung abiotischer und biotischer Ressourcen Anwendung. Da die Nutzung von Ressourcen auch auf Macro-Ebene betrachtet werden muss, wurde eine Methode zur Bewertung abiotischer Ressourcen auf regionaler Ebene entwickelt, die 25 Indikatoren für die Bewertung der Kritikalität (Verfügbarkeit von Ressourcen und Vulnerabilität der Region) und der gesellschaftlichen Akzeptanz zur Verfügung stellt. Verschiedene Fallstudien wurden durchgeführt um die Anwendbarkeit der entwickelten Methoden aufzuzeigen und zu verdeutlichen, warum eine umfassende Bewertung der Ressourcennutzung notwendig ist. Die Fallstudien umfassen u.a. die Bewertung eines Smartphones, Pkw-Herstellung und Biokraftstoffe. Die Anwendbarkeit der Methoden wird zudem erhöht, indem Indikatorwerte für 36 Metalle und 4 fossile Rohstoffe zur Verfügung gestellt werden. Die Bewertung der Nutzung abiotischer und biotischer Ressourcen auf Produkt- und regionaler Ebene wird mit dieser Dissertation signifikant verbessert, indem vier wissenschaftliche Methoden zur robusten und umfassenden Bewertung aller drei Nachhaltigkeitsdimensionen bereitgestellt werden. Resources are the basis for a thriving industrial and technological development and therefore for prosperity of present and future generations. With increasing resource use, challenges with regard to (physical and socio-economic) availability of abiotic and biotic resources and raw materials, pollution of the environment as well as social impacts associated with resource extraction and use arise. To evaluate the success of strategies managing resource use more efficiently and sustainably methodologies are required to comprehensively assess resource use and related impacts. This thesis provides four methodologies to improve the assessment of abiotic and biotic resource use in the context of sustainability on product and regional level. For the method to assess abiotic resources use on product level overall 21 aspects are considered as relevant and indicator for quantification are provided. In order to determine socio-economic supply chain restrictions a new approach is developed, considering geopolitical, political and regulatory aspects affecting resource extraction and use. Further, screening indicators are established to evaluate the societal acceptance of resources with regard to compliance with social and environmental standards. To assess the availability of terrestrial biotic resources in product systems a comprehensive methodology is established, which includes 25 indicators. Further, an approach is proposed to combine assessment methodologies in a consistent way. This approach is applied to the developed method of this thesis leading to a combined methodology. The use of resources also has to be considered on macro-economic. Thus, a methodology is developed providing 25 indicators for the two dimensions criticality, consisting of the sub-dimensions (physical and socio-economic) availability and vulnerability, as well as societal acceptance. Several case studies are carried out to demonstrate the applicability of the developed methods and to confirm the need for a comprehensive assess of resource use on micro and macro level, e.g. case studies for smart phones and cars, for biofuels produced from rapeseed and soybean. The applicability of the methodologies is further enhanced by providing indicator results for 36 metals and four fossil raw materials. The assessment of abiotic and biotic resource use on product and regional level is improved significantly by establishing four scientifically robust yet applicable methodologies, which consider multiple aspects of resource use in all three sustainability dimensions.

A reduction in the availability of phosphate rock resources for fertiliser production coincides with an increase in phosphorus-rich dairy wastewater in Europe. This confluence of events has led to the development of technologies for phosphorus recovery from dairy wastewater and the use of the products as fertilisers in agriculture. This thesis aims to contribute both to the technical development of this emerging technical system and methodological development of assessing the sustainability of it with regard to (1) the identification and selection of sustainability indicators, and (2) the assessment of life cycle environmental impacts. The thesis describes an approach developed for identifying and selecting sustainability indicators by reviewing scientific documents and interviews as well as an approach employed for performing a meta-analysis of previously published life cycle assessment results to cope with lack of inventory data. The employed method for indicator selection narrows down an initial set of 382 sustainability indicators identified in the literature to 26, which were considered representative and useful for the assessment of the considered innovative conceptual system. The meta-analysis results suggested that the examined phosphorus recovery technologies exhibited a lower global warming potential and cumulative energy demand than those of dairy wastewater treatment processes and that those technologies recovering phosphorus from the liquid phase had lower impacts than those recovering phosphorus from sludge or ash.

The core aim of this thesis was to quantify the effects of co-digesting forage silages with animal slurries on methane yields and to investigate if antagonistic or synergistic outcomes occur. In order to complete this assessment, the economic impacts of changing forage silage characteristics, of changing the mixing ratios of forage silage and cattle slurry in binary mixtures (and the presence of synergy or antagonism) and of changing the costs of providing these feedstocks for anaerobic digestion (AD) on the cost of methane production in an on-farm AD facility were accessed. An initial objective, however, was to define an optimal methodology for laboratory-scale anaerobic digestion, specifically to determine the impact of altering the headspace volume within incubation bottles and the overhead pressure measurement and release (OHPMR) frequency on methane yield using a manual manometric biochemical methane potential (mBMP) batch digestion method. Two anaerobic batch co-digestion experiments were conducted with forage silages and animal slurries. In the first experiment, oven-dried perennial ryegrass (harvested at two growth stages) or red clover (harvested at two growth stages) silages and cattle slurry were co-digested. Each binary mixture had synergistic effects which resulted in 2.8-7.5% higher methane yields than predicted from mono-digestion of individual substrates. In the second experiment, cattle slurry (two types) or pig slurry was co-digested with undried perennial ryegrass silages (harvested at two growth stages). Each silage and slurry mixture had antagonistic effects which resulted in methane yields 5.7-7.6% below those predicted from mono-digestion of individual substrates. In the initial experiment and in order to broaden the conditions under which the assessment was made, the biogas and methane yields of cellulose, barley grain, grass silage and cattle slurry were determined in response to three incubation bottle headspace volumes and four OHPMR frequencies. The methane yields of barley, silage and slurry were also compared with those from an automated volumetric method (i.e. AMPTS). Headspace volume and OHPMR frequency effects on biogas yield were mediated mainly through headspace pressure, with the latter having a negative effect on the biogas yield measured but relatively little effect on methane yield. Two mBMP treatments that produced methane yields equivalent to AMPTS were identified. Economic modelling results showed significant impacts of AD feedstock characteristics and their provision cost on the cost of methane production in an AD facility. The feedstock provision cost contributed about half of the total cost of methane production when the AD facility solely operated on grass silage. The total cost of methane produced from mono-digestion of cattle slurry that was supplied free of charge was more than double the cost of methane produced from grass silage. For co-digestion of grass silage and cattle slurry, the total cost of methane production progressively increased as the proportion of slurry in the co-digested mixture increased. Antagonistic and synergistic methanogenesis resulted in a corresponding 6% higher and 5% lower total cost of methane production during co-digestion of grass silage and cattle slurry (at a silage:slurry volatile solids ratio of 0.8:0.2) compared to the binary mixture without these effects.

We analyse ex-ante the effects of diet recommendations in France, Finland and Denmark. The simulation approach combines a behavioural model of adjustment to dietary constraints, an epidemiological model and an LCA model. We conclude that for the three countries: 1- The promotion of several diet recommendations would improve social welfare; 2- Healthy-eating recommendations targeting consumption of saturated fat, fruits/vegetables and salt should be prioritized for promotion; 3- Although synergies dominate, trade-offs between environmental and health objectives may occur in Finland and Denmark; and 4- The taste/utility cost of dietary change imposed on consumers should be included in the welfare analysis of diet recommendations. none

The IPCC stresses the importance of achieving net-zero CO2 emissions worldwide by 2050 and natural climate solutions, particularly carbon farming, can play a significant role in this goal. However, current markets do not account for environmental externalities, which creates a mismatch between individual costs and societal benefits. Payment systems linked to carbon farming practices could help bridge this gap. Research is essential to develop effective agricultural carbon markets, and this study focuses on the opportunities and challenges faced by smallholder farmers in these markets. The research examines four areas: agricultural markets as a funding source for carbon farming, payments for carbon sequestration, opportunities for smallholder farmers, and cost-effective monitoring and verification of carbon stocks. Further research is needed to monitor carbon sequestration accurately, reduce GHG emissions, and develop institutional arrangements to promote sustainable production methods in Africa.

This work, carried out at Research Centre for Industrial Crops of CREA in Rovigo, aimed at assessing the performance of a modified combine header and seed losses during late harvest of 5 hemp varieties. The renewed interest in Italy for hemp cultivation is aimed at seed production unlike the traditional cultivation aimed at fibre. However, it must face the lack of monoecious varieties suitable for Italian environment. In the case of dioecious varieties, traditionally cultivated in Italy for fibre production, the high crop height makes it difficult the mechanical harvesting with conventional combines. Similarly, the uneven height recorded in the case of imported varieties for seed production, requires adjusting continuously the height of the combine header. So, modified combine headers capable of be moved upwards and downwards by a lift have been developed. The aim is to avoid the risk of possible clogging of threshing apparatus introducing big pieces of stalks. Moreover, in this way, the stalks can be harvested separately in order to increase the income for farmers. In view of valorise the whole plant, as multipurpose crop, the right harvest time should be a trade-off between seed yield and fibre quality. Early harvesting guaranties a good fibre quality but causes also the harvest of seed not completely ripened. On the contrary delaying the harvest reduces the seed yield due to seed loss for dehiscence. In addition to losses for dehiscence prior to harvest, the trials aimed at distinguishing the loss incurred during harvest attributable both to crop disturbance by combine header and to the setting of the combine’s threshing and separation elements. Proceedings of the 28th European Biomass Conference and Exhibition, 6-9 July 2020, Virtual, pp. 197-200