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  • Energy Research
  • DK
  • University of Copenhagen

  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: orcid Moseley, Gina E.;
    Moseley, Gina E.
    ORCID
    Harvested from ORCID Public Data File

    Moseley, Gina E. in OpenAIRE
    Rosvold, Jorgen; Gotfredsen, Anne Birgitte; Hajdas, Irka; +4 Authors

    Gyrfalcon (Falco rusticolus) is the largest falcon in the world. It inhabits a wide range of climate zones in the Northern Hemisphere, from boreal forests in the south of its range to the arid polar deserts of the High Arctic. In Greenland, because of the harsh, remote environments in which gyrfalcons live, research related to the contemporary and pre-modern periods has been limited to the north-west, central west and central east coasts, with no specific investigations being conducted for the north-east. Here, we report the first pre-modern record of a gyrfalcon in north-east Greenland, located at 80.4°N in Kronprins Christian Land. Skin tissue from a decaying gyrfalcon’s body was radiocarbon dated to 769–944 CE (common era) using a terrestrial-only calibration curve, and 1182–1456 CE using a marine-only calibration curve. Since the gyrfalcon has a mixed terrestrial/marine diet, the actual age can be said to belong between these two groups. This limited data, therefore places the presence of the gyrfalcon in north-east Greenland during a period of prolonged elevated temperatures and climate stress associated with the Medieval Climate Anomaly. Whether the gyrfalcon was part of a larger population or a straggler, and whether the species survived the whole of the Medieval Climate Anomaly in north-east Greenland, is unknown. Polar Research, 38 ISSN:0800-0395 ISSN:1751-8369

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Polar Researcharrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Polar Research
    Article . 2019 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Polar Research
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    Polar Research
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    Research Collection
    Article . 2019
    License: CC BY NC
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    Polar Research
    Article . 2019
    Data sources: DOAJ
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Research Collection
    Article . 2019
    License: CC BY NC
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Polar Researcharrow_drop_down
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      Polar Research
      Article . 2019 . Peer-reviewed
      Data sources: Crossref
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Polar Research
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      Polar Research
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      Research Collection
      Article . 2019
      License: CC BY NC
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      Polar Research
      Article . 2019
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Research Collection
      Article . 2019
      License: CC BY NC
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: orcid Bentsen, Niclas Scott;
    Bentsen, Niclas Scott
    ORCID
    Harvested from ORCID Public Data File

    Bentsen, Niclas Scott in OpenAIRE

    Abstract In later years the potential contribution of forest bioenergy to mitigate climate change has been increasingly questioned due to temporal displacement between CO 2 emissions when forest biomass is used for energy and subsequent sequestration of carbon in new biomass. Also disturbance of natural decay of dead biomass when used for energy affect the carbon dynamics of forest ecosystems. These perturbations of forest ecosystems are summarized under the concept of carbon debt and its payback time. Narrative reviews demonstrate that the payback time of apparently comparable forest bioenergy supply scenarios vary by up to 200 years allowing amble room for confusion and dispute about the climate benefits of forest bioenergy. This meta-analysis confirm that the outcome of carbon debt studies lie in the assumptions and find that methodological rather than ecosystem and management related assumptions determine the findings. The study implies that at the current development of carbon debt methodologies and their lack of consensus the concept in it-self is inadequate for informing and guiding policy development. At the management level the carbon debt concept may provide valuable information directing management principles in a more climate benign directions.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Renewable and Sustai...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Renewable and Sustainable Energy Reviews
    Article . 2017 . Peer-reviewed
    License: Elsevier TDM
    Data sources: Crossref
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Renewable and Sustai...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Renewable and Sustainable Energy Reviews
      Article . 2017 . Peer-reviewed
      License: Elsevier TDM
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • Authors: orcid bw Mette Fog Olwig;
    Mette Fog Olwig
    ORCID
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    Mette Fog Olwig in OpenAIRE
    orcid bw Aske Skovmand Bosselmann;
    Aske Skovmand Bosselmann
    ORCID
    Derived by OpenAIRE algorithms or harvested from 3rd party repositories

    Aske Skovmand Bosselmann in OpenAIRE
    orcid bw Kwadwo Owusu;
    Kwadwo Owusu
    ORCID
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    Kwadwo Owusu in OpenAIRE

    Este libro de acceso abierto proporciona una perspectiva multidisciplinaria sobre el potencial de los árboles para mitigar los impactos negativos del cambio climático en la producción de cacao Ce livre en libre accès offre une perspective multidisciplinaire sur le potentiel des arbres pour atténuer les impacts négatifs du changement climatique sur la production de cacao This open access book provides multidisciplinary perspective on the potential of trees to mitigate negative impacts of climate change on cocoa production يوفر هذا الكتاب المفتوح منظورًا متعدد التخصصات حول إمكانات الأشجار للتخفيف من الآثار السلبية لتغير المناخ على إنتاج الكاكاو

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: orcid Morales, Juan M.;
    Morales, Juan M.
    ORCID
    Harvested from ORCID Public Data File

    Morales, Juan M. in OpenAIRE
    Zugno, Marco; orcid Pineda Morente, Salvador;
    Pineda Morente, Salvador
    ORCID
    Harvested from ORCID Public Data File

    Pineda Morente, Salvador in OpenAIRE
    orcid Pinson, Pierre;
    Pinson, Pierre
    ORCID
    Harvested from ORCID Public Data File

    Pinson, Pierre in OpenAIRE

    This letter proposes a new merit order for the dispatch of stochastic production in forward markets (e.g., day-ahead markets). The proposed merit order considers not only the marginal cost of the stochastic generating unit, which is often very low or zero, but also the projected cost of balancing its energy deviations during the real-time operation of the power system. We show, through an illustrative example, that the proposed merit order leads to increased market efficiency as the penetration of stochastic generation in the electricity market grows.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ IEEE Transactions on...arrow_drop_down
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    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    IEEE Transactions on Power Systems
    Article . 2014 . Peer-reviewed
    License: IEEE Copyright
    Data sources: Crossref
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ IEEE Transactions on...arrow_drop_down
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      IEEE Transactions on Power Systems
      Article . 2014 . Peer-reviewed
      License: IEEE Copyright
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: orcid Zhao, Qi;
    Zhao, Qi
    ORCID
    Harvested from ORCID Public Data File

    Zhao, Qi in OpenAIRE
    Pedersen, Christian Marcus; Wang, Jiamin; Liu, Rui; +5 Authors

    The component analysis and structure characterization of complex mixtures of biomass conversion remain a challenging work. Hence, developing effective and easy to use techniques is necessary. Diffusion-ordered NMR spectroscopy (DOSY) is a non-selective and non-invasive method capable of achieving pseudo-separation and structure assignments of individual compounds from biomass mixtures by providing diffusion coefficients (D) of the components. However, the conventional 1H DOSY NMR is limited by crowded resonances when analyzing complex mixtures containing similar chemical structure resulting in similar coefficient. Herein we describe the application of an advanced diffusion NMR method, Pure Shift Yielded by CHirp Excitation DOSY (PSYCHE-iDOSY), which can record high-resolution signal diffusion spectra efficiently separating compounds in model and genuine mixture samples from cellulose, hemicellulose and lignin. Complicated sets of isomers (d-glucose/d-fructose/d-mannose and 1,2-/1,5-pentadiol), homologous compounds (ethylene glycol and 1,2-propylene glycol), model compounds of lignin, and a genuine reaction system (furfuryl alcohol hydrogenolysis with ring opening) were successfully separated in the diffusion dimension. The results show that the ultrahigh-resolution DOSY technique is capable of detecting and pseudo-separating the mixture components of C5/C6 sugar conversion products and its derivative hydrogenation/hydrogenolysis from lignocellulose biomass.

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    Green Energy & Environment
    Article . 2023 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Green Energy & E...arrow_drop_down
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      Green Energy & Environment
      Article . 2023 . Peer-reviewed
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: orcid Singh, Raushan Kumar;
    Singh, Raushan Kumar
    ORCID
    Harvested from ORCID Public Data File

    Singh, Raushan Kumar in OpenAIRE
    orcid Tiwari, Manish Kumar;
    Tiwari, Manish Kumar
    ORCID
    Harvested from ORCID Public Data File

    Tiwari, Manish Kumar in OpenAIRE
    Kim, Dongwook; orcid Kang, Yun Chan;
    Kang, Yun Chan
    ORCID
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    Kang, Yun Chan in OpenAIRE
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    An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temperature of 40 °C and pH of 5.5. Using oat-spelt xylan, the determined K m, V max, and k cat/K m values were 0.243 mg ml⁻¹, 4,530 U mg⁻¹ protein, and 7,640 ml s⁻¹ mg⁻¹, respectively. A homology model and sequence analysis of XylCg, along with the biochemical properties, confirmed that XylCg belongs to the GH11 family. Rice straw pretreated with XylCg showed 30 % higher conversion yield than the rice straw pretreated with a commercial xylanase. Although xylanases have been characterized from fungal and bacterial sources, C. globosum XylCg is distinguished from other xylanases by its high catalytic efficiency and its effectiveness in the pretreatment of lignocellulosic biomass.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Applied Microbiology...arrow_drop_down
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    Applied Microbiology and Biotechnology
    Article . 2012 . Peer-reviewed
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      Applied Microbiology and Biotechnology
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    Authors: orcid Morgane Thomsen;
    Morgane Thomsen
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    orcid Jens Juul Holst;
    Jens Juul Holst
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    Anna Molander; orcid Kristian Linnet;
    Kristian Linnet
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    Kristian Linnet in OpenAIRE
    +2 Authors

    Preclinical studies in rodents have demonstrated inhibitory effects of glucagon-like peptide-1 (GLP-1) receptor stimulation on alcohol consumption. The effects of GLP-1 receptor stimulation on alcohol intake in primates have not been investigated.We performed placebo-controlled studies on the effects of the GLP-1 receptor agonists exenatide and liraglutide on alcohol consumption in alcohol-preferring male African vervet monkeys. Monkeys selected for voluntary alcohol drinking were observed for at least 10 days of baseline drinking and allocated to drug or vehicle (n = 11-12 per group) balanced with respect to alcohol intake. Monkeys had access to alcohol 4 h/day. In a first study, monkeys were treated with exenatide 0.04 mg/kg or vehicle once weekly for 5 weeks to obtain steady-state plasma levels. In a second study, monkeys were treated daily with liraglutide (increasing dosing, 10 to 50 μg/kg/day) or vehicle over 2 weeks. In both studies, access to alcohol was suspended during drug up-titration. Then, alcohol was again made available 4 h/day and treatment was continued for 2 weeks, during which alcohol intake was recorded. Observation of alcohol intake was continued for a week of drug washout.Liraglutide and to a lesser extent exenatide significantly reduced alcohol consumption without causing any signs of emesis and with no effect on water intake as compared to vehicle.The present study demonstrates for the first time that GLP-1 receptor agonists can reduce voluntary alcohol drinking in non-human primates. The data substantiate the potential usefulness of GLP-1 receptor agonists in the treatment of alcohol use disorder.

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    Psychopharmacology
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    Psychopharmacology
    Article . 2018 . Peer-reviewed
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      Psychopharmacology
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      Psychopharmacology
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    Authors: Even Tjørve; Rüdiger Otto; orcid Lea de Nascimento;
    Lea de Nascimento
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    Lea de Nascimento; +7 Authors

    AbstractAlthough the role that Pleistocene glacial cycles have played in shaping the present biota of oceanic islands world‐wide has long been recognized, their geographical, biogeographical and ecological implications have not yet been fully incorporated within existing biogeographical models. Here we summarize the different types of impacts that glacial cycles may have had on oceanic islands, including cyclic changes in climate, shifts in marine currents and wind regimes and, especially, cycles of sea level change. The latter have affected geographical parameters such as island area, isolation and elevation. They have also influenced the configurations of archipelagos via island fusion and fission, and cycles of seamount emergence and submergence. We hypothesize that these sea level cycles have had significant impacts on the biogeographical processes shaping oceanic island biotas, influencing the rates and patterns of immigration and extinction and hence species richness. Here we provide a first step toward the development of a glacial‐sensitive model of island biogeography, representing the tentative temporal evolution of those biogeographical parameters during the last glacial cycle. From this reasoning we attempt to derive predictions regarding the imprint of sea level cycles on genetic, demographic or biogeographical patterns within remote island biotas.

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    Global Ecology and Biogeography
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    Global Ecology and Biogeography
    Article . 2015 . Peer-reviewed
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      Global Ecology and Biogeography
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      Global Ecology and Biogeography
      Article . 2015 . Peer-reviewed
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    Authors: Lassen, Rune Nørbæk; orcid Plampin, M.;
    Plampin, M.
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    Plampin, M. in OpenAIRE
    Sakaki, T.; orcid Illangasekare, T. H.;
    Illangasekare, T. H.
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    Illangasekare, T. H. in OpenAIRE
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    Abstract There is a concern that underground stored CO 2 may potentially escape and intrude shallow aquifers that may lead to degradation of the water quality and possible mobilization of metals. Moreover migrating CO 2 gas may invade buildings causing lethal conditions for humans. A better understanding of gaseous CO 2 migration in heterogeneous aquifer systems at low pressure will help in risk assessment of Carbon Capture and Storage (CCS). We performed complementary 1D column and 2D tank experiments by injecting gaseous CO 2 at three different rates into constructed heterogeneous porous media in laboratory setups. Soil moisture sensors were installed in the test systems to monitor the movement of the gaseous phase. The generated experimental data was analyzed using the numerical multiphase modeling code T2VOC. The results confirmed that large-scale heterogeneity controls overall gaseous CO 2 migration in porous media while processes occurring at smaller scale are of significance for gas saturations. Monitoring the exact gaseous movement and concentrations is difficult even in a constructed heterogeneous medium and point measurements are not always capturing the full dynamics of the system. The numerical model provided good estimates of the general flow of the gaseous phase around larger heterogeneous features and estimated the total amount of gaseous CO 2 in the test system to a good approximation. However, the numerical model was not able to adequately describe the processes occurring at smaller scale including unstable gaseous movement at low flow rates and dissolution of CO 2 .

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    International Journal of Greenhouse Gas Control
    Article . 2015 . Peer-reviewed
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      International Journal of Greenhouse Gas Control
      Article . 2015 . Peer-reviewed
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    Authors: orcid BARER, M;
    BARER, M
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    BARER, M in OpenAIRE
    LYON, H;

    The effects of formaldehyde, glutaraldehyde, methanol, Clarke's fixative and microwave irradiation on the quantitative staining of proteins (Naphthol Yellow S) and nucleic acids (Ethyl Green-Pyronin) in a cell culture system have been investigated. Overall, glutaraldehyde rapidly yielded the highest and most consistent levels of staining when compared to all other chemical fixatives. Although microwave irradiation was found to be uneven, 4 min exposure to 700W was found to give higher levels of protein staining than those achieveable with glutaraldehyde. Time-dependent processes were observed with all procedures. In addition, dissociations in the trends of protein and nucleic acid staining were observed. It is suggested that these results demonstrate fixation events that have not previously been resolved from the effects of reagent penetration into tissue blocks.

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    The Histochemical Journal
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      The Histochemical Journal
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