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To understand the effects of physicochemical factors on nitrite transformation by microalgae, a lipid-rich Chlorella with high nitrite tolerance was cultured with 8 mmol/l sodium nitrite as sole nitrogen source under different conditions. The results showed that nitrite transformation was mainly dependent on the metabolic activities of algal cells rather than oxidation of nitrite by dissolved oxygen. Light intensity, temperature, pH, NaHCO3 concentrations, and initial cell densities had significant effects on the rate of nitrite transformation. Single-factor experiments revealed that the optimum conditions for nitrite transformation were light intensity: 300 μmol/m(2); temperature: 30°C; pH: 7-8; NaHCO3 concentration: 2.0 g/l; and initial cell density: 0.15 g/l; and the highest nitrite transformation rate of 1.36 mmol/l/d was achieved. There was a positive correlation between nitrite transformation rate and the growth of Chlorella. The relationship between nitrite transformation rate (mg/l/d) and biomass productivity (g/l/d) could be described by the regression equation y = 61.3x (R(2) = 0.9665), meaning that 61.3 mg N element was assimilated by 1.0 g dry biomass on average, which indicated that the nitrite transformation is a process of consuming nitrite as nitrogen source by Chlorella. The results demonstrated that the Chlorella suspension was able to assimilate nitrite efficiently, which implied the feasibility of using flue gas for mass production of Chlorella without preliminary removal of NOX.

Different species within the same community may exhibit distinct phenological responses to climate change, so it is necessary to study species differences in the green-up date among abundant species within a wide area, and a suitable phenology model should be introduced to explain the associated climate-driven mechanism. Although various models have been developed, very few studies have aimed to compare their efficiency and robustness, and the relative contributions of climate driving factors have not been sufficiently examined. We analyzed phenology data for 12 species across 17 stations in Inner Mongolia and found that essential spatiotemporal and interspecies differences existed in the green-up date. Five process-based models were established for each species and their performance was comprehensively evaluated. The two-phase models (sequential model, parallel model, unified model and unified model combined with precipitation driving) generally performed better than the one-phase model (thermal time model), and the model considering precipitation performed the best, which indicates that it is necessary to introduce the chilling effect and precipitation driving effect to improve the model accuracy in arid environments. We proposed a method to estimate the contribution rates of various climate driving factors, and significant differences in the relative demand for the various climate driving factors among different species were clearly revealed. The results indicated that for natural vegetation in Inner Mongolia, the need for the chilling and temperature driving is relatively high, and the precipitation driving is very important for herbaceous vegetation, which leads to considerable spatial and interspecies differences in green-up date. We demonstrated the feasibility of quantitatively evaluating the contributions of different climate driving factors with a process-based model, and the contradiction in phenological changes among different studies may eventually be clarified.

AbstractA bioreactor system composed of a stirred tank and three tubular bioreactors in series was established, and continuous ethanol fermentation was carried out using a general Saccharomyces cerevisiae strain and a very high gravity medium containing 280 g L−1 glucose, supplemented with 5 g L−1 yeast extract and 3 g L−1 peptone. Sustainable oscillations of glucose, ethanol, and biomass were observed when the tank was operated at the dilution rate of 0.027 h−1, which significantly affected ethanol fermentation performance of the system. After the tubular bioreactors were packed with 1/2″ Intalox ceramic saddles, the oscillations were attenuated and quasi‐steady states were achieved. Residence time distributions were studied for the packed bioreactors by the step input response technique using xylose as a tracer, which was added into the medium at a concentration of 20 g L−1, indicating that the backmixing alleviation assumed for the packed tubular bioreactors could not be established, and its contribution to the oscillation attenuation could not be verified. Furthermore, the role of the packing's yeast cell immobilization in the oscillation attenuation was investigated by packing the tubular bioreactors with packings with significant difference in yeast cell immobilization effects, and the experimental results revealed that only the Intalox ceramic saddles and wood chips with moderate yeast cell immobilization effects could attenuate the oscillations, and correspondingly, improved the ethanol fermentation performance of the system, while the porous polyurethane particles with good yeast cell immobilization effect could not. And the viability analysis for the immobilized yeast cells illustrated that the extremely lower yeast cell viability within the tubular bioreactors packed with the porous polyurethane particles could be the reason for their inefficiency, while the yeast cells loosely immobilized onto the surfaces of the Intalox ceramic saddles and wood chips could be renewed during the fermentation, guaranteeing their viability and making them more efficient in attenuating the oscillations. The packing Raschig rings without yeast cell immobilization effect did not affect the oscillatory behavior of the tubular bioreactors, further supporting the role of the yeast cell immobilization in the oscillation attenuation. Biotechnol. Bioeng. 2009;102: 113–121. © 2008 Wiley Periodicals, Inc.

Abstract In this study, intergeneric grafting was employed between Populus cathayana and Salix rehderiana to investigate the grafting compatibility of the two Salicaceae plants and to reveal whether grafting can improve their drought resistance. Under different grafting combinations (P. cathayana scion with P. cathayana rootstock, P/P; P. cathayana scion with S. rehderiana rootstock, P/S; S. rehderiana scion with S. rehderiana rootstock, S/S; and S. rehderiana scion with P. cathayana rootstock, S/P), the survival and growth rate, biomass accumulation and allocation, photosynthetic traits, carbon isotope composition (δ13C), relative water content (RWC) and non-structural carbohydrates (NSCs) were measured. The results showed that the grafting compatibility between P. cathayana and S. rehderiana was very high, as the survival rates ranged from 76% to 100% under different grafting combinations. Drought significantly decreased growth, biomass accumulation, photosynthetic pigment contents, net photosynthesis rates (Pn) and RWC, and increased δ13C in all grafting combinations. Under drought stress, biomass accumulation, total chlorophyll, transpiration rate (E) and Pn were higher in P/P and P/S than in S/S and S/P. Compared with P/P, the growth rate, biomass accumulation, root/aboveground ratio (R/A ratio), carotenoid, RWC, starch and total soluble sugar (TSS) of P/S were less affected by drought. The height growth rate (GRH), R/A ratio, carotenoid, chlorophyll a, total chlorophyll, WUEi and TSS of S/P were lower than those of S/S under water-limited conditions. Moreover, a principal component analysis indicated that P/S and S/S had higher drought resistance than P/P and S/P under water deficits. The used method allows combining specific advantageous traits from P. cathayana and S. rehderiana, which may be a highly useful tool to enhance drought resistance in the cultivation of Salicaceae plants.

Abstract Phosphorus (P) is an important nutrient that can restrict plant growth. However, the influence of P deficiency on elemental homeostasis and application of the growth rate hypothesis in higher plants remain to be assessed. Two shrubs, Zygophyllum xanthoxylum and Nitraria tangutorum, were used as experiment material and subjected to five P addition treatments: 0, 17.5, 35.0, 52.5 and 70.0 mg P·kg−1 soil. The biomass and relative growth rate of Z. xanthoxylum did not change with altered P supply. There was no significant difference in P concentration among the treatments for Z. xanthoxylum, but N. tangutorum showed an upward trend. The P stoichiometric homeostasis of Z. xanthoxylum was higher than that of N. tangutorum. For Z. xanthoxylum, available P in the rhizosphere improved significantly under extreme P deficiency conditions, and P concentrations in all treatments were lower than in N. tangutorum, showing that Z. xanthoxylum had stronger P absorption and P utilization capacity. No relationships between growth rate and C:N:P ratios were found in Z. xanthoxylum. The strong P efficiency, and high and stable dry matter accumulation, are likely contributors in maintaining stoichiometric homeostasis. In addition, the relatively high biomass accumulation and high P utilization efficiency for Z. xanthoxylum does not support the growth rate hypothesis for this species.

This study investigated the influence of broadleaf and conifer vegetation on soil microbial communities in a distinct vertical distribution belt in Northeast China. Soil samples were taken at 0-5, 5-10 and 10-20 cm depths from four vegetation types at different altitudes, which were characterized by poplar (Populus davidiana) (1250-1300 m), poplar (P. davidiana) mixed with birch (Betula platyphylla) (1370-1550 m), birch (B. platyphylla) (1550-1720 m), and larch (Larix principis-rupprechtii) (1840-1890 m). Microbial biomass and community structure were determined using the fumigation-extraction method and phospholipid fatty acid (PLFA) analysis, and soil fungal community level physiological profiles (CLPP) were characterized using Biolog FF Microplates. It was found that soil properties, especially soil organic carbon and water content, contributed significantly to the variations in soil microbes. With increasing soil depth, the soil microbial biomass, fungal biomass, and fungal catabolic ability diminished; however, the ratio of fungi to bacteria increased. The fungal ratio was higher under larch forests compared to that under poplar, birch, and their mixed forests, although the soil microbial biomass was lower. The direct contribution of vegetation types to the soil microbial community variation was 12%. If the indirect contribution through soil organic carbon was included, variations in the vegetation type had substantial influences on soil microbial composition and diversity.

The response of plants to climate change has become a topical issue. However, there is no consensus on the synergistic processes of the canopy and trunk growth within different vegetation types, or on the consistency of the response of the canopy and trunk to climate change. This paper is based on Normalized Difference Vegetation Index (NDVI), tree-ring width index (TRW) and climate data from the Irtysh River basin, a sensitive area for climate change in Central Asia. Spatial statistical methods and correlation analysis were used to analyze the spatial and temporal trends of plants and climate, and to reveal the differences in the canopy and trunk response mechanisms to climate within different vegetation types. The results show a warming and humidifying trend between 1982 and 2015 in the study area, and NDVI and TRW increases in different vegetation type zones. On an interannual scale, temperature is the main driver of the canopy growth in alpine areas and precipitation is the main limiting factor for the canopy growth in lower altitude valley and desert areas. The degree of response of the trunk to climatic factors decreases with increasing altitude, and TRW is significantly correlated with mean annual temperature, precipitation and SPEI in desert areas. On a monthly scale, the earlier and longer growing season due to the accumulation of temperature and precipitation in the early spring and late autumn periods contributes to two highly significant trends of increase in the canopy from March to May and August to October. Climatic conditions during the growing season are the main limiting factor for the growth of the trunk, but there is considerable variation in the driving of the trunk in different vegetation type zones. The canopy growth is mainly influenced by climatic factors in the current month, while there is a 1–2-month lag effect in the response of the trunk to climatic factors. In addition, the synergy between the canopy and the trunk is gradually weakened with increasing altitude (correlation coefficient is 0.371 in alpine areas, 0.413 in valley areas and 0.583 in desert areas). These findings help to enrich the understanding of the response mechanisms to climate change in different vegetation type zones and provide a scientific basis for the development of climate change response measures in Central Asia.

Global patterns of biodiversity and comparisons between tropical and temperate ecosystems have pervaded ecology from its inception. However, the urgency in understanding these global patterns has been accentuated by the threat of rapid climate change. We apply an adaptive model of environmental tolerance evolution to global climate data and climate change model projections to examine the relative impacts of climate change on different regions of the globe. Our results project more adverse impacts of warming on tropical populations due to environmental tolerance adaptation to conditions of low interannual variability in temperature. When applied to present variability and future forecasts of precipitation data, the tolerance adaptation model found large reductions in fitness predicted for populations in high-latitude northern hemisphere regions, although some tropical regions had comparable reductions in fitness. We formulated an evolutionary regional climate change index (ERCCI) to additionally incorporate the predicted changes in the interannual variability of temperature and precipitation. Based on this index, we suggest that the magnitude of climate change impacts could be much more heterogeneous across latitude than previously thought. Specifically, tropical regions are likely to be just as affected as temperate regions and, in some regions under some circumstances, possibly more so.