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Land use and climate change always induce significant changes in various parameters of the hydrologic cycle (e.g., surface runoff, infiltration, evapotranspiration). The Wadi El-Assiuti downstream area in the Eastern Desert of Egypt is one of the most promising areas for development that is suffering from insufficient water availability and inadequate water quality for different purposes. The main goal of this research is to evaluate the changes in groundwater quality, land use, and climate in association with geology and flooding during three periods within the years 1997–2019 in the downstream portion of Wadi El-Assiuti in the Eastern Desert of Egypt, using spatiotemporal variation associated with groundwater hydrochemical analysis and GIS techniques. About 133 groundwater samples were collected to examine groundwater quality changes over time. Different groundwater quality indices were calculated, and the results show that TDS levels of groundwater in the study area ranged between 1080–2780 mg/L, 672–4564 mg/L, and 811–6084 mg/L, while SAR levels varied within 6.15–15.34, 1.83–28.87, and 1.43–30.57 for the years 1997, 2007, and 2019, respectively. Both RSBC and SSP values exhibited significantly increasing trends over time. KR values were within 1.36–4.06 in 1997, 0.58–14.09 in 2007, and 0.35–14.92 in 2019; MAR values were within 6.9–45.2 in 1997, 20.79–71.5 in 2007, and 17.71–75.81 in 2019; and PI values were within 60.16–83 in 1997, 45.56–101.03 in 2007, and 42.51–148.88 in 2019. Across the entire study area, ongoing land use changes increased from 1.1% in 1997 to 4.1% in 2019. Findings pointed to the significant contribution of the deep Nubian Sandstone Aquifer to the groundwater aquifer at Wadi El-Assiuti through fractures and deep faults. Given the climatic conditions from 1997–2019, these changes may have affected water quality in shallow aquifers, especially with increasing evaporation. Realizing the spatiotemporal variation of the aquifer recharge system, land use development, and climate change clearly would help in water resource management. This study revealed that flooding events, deep-seated geologic structures, and land use development associated with human activities have the highest impact on groundwater quality.

Institutional linkages and information flow between agricultural organizations play a critical role in addressing sustainability issues and promoting agrarian innovation. The aim of this study was to evaluate institutional relations and information between the various actors within the agricultural knowledge and information system (AKIS). The study focused on eight actors within the AKIS in Dakahlia governorate of Egypt, namely policy, extension, research, agricultural cooperatives, higher education, secondary education, credit, and the private sector. Thus, the survey sample included 11 representatives of each actor with 88 respondents. Data were collected by a standardized questionnaire distributed online. The graph theoretical technique was used for the quantitative assessment of information flow and institutional linkages established among actors. The findings indicated that agricultural extension ranked first about their real cause and effect on the rest of the system, having a value of 7.95. Two critical information pathways within the AKIS sustained innovation outcomes: (1) higher education–extension–agricultural cooperatives, (2) research–extension–agricultural cooperatives. The results also revealed that agricultural cooperatives ranked second after the extension component on the extent of supplying information to other members in the AKIS, with a value of 4.8. In contrast, the highest component received information from other components (7.6). By analyzing institutional linkages and information flow, this article gives insights to policymakers on the mechanisms that still need to be strengthened and the information gaps between actors to address the challenges of sustainable rural development.

Abstract Rice straw was subjected to fungal pretreatment using Pleurotus ostreatus and Trichoderma reesei to improve its biodegradability and methane production via solid-state anaerobic digestion (SS-AD). Effects of moisture content (65%, 75% and 85%), and incubation time (10, 20 and 30 d) on lignin, cellulose, and hemicellulose degradation during fungal pretreatment and methane yield during anaerobic digestion were assessed via comparison to untreated rice straw. Pretreatment with P. ostreatus was most effective at 75% moisture content and 20 d incubation resulting in 33.4% lignin removal and a lignin/cellulose removal ratio (selectivity) of 4.2. In comparison Trichoderma reesei was most effective at 75% moisture content and 20 d incubation resulting in 23.6% lignin removal and a lignin/cellulose removal ratio (selectivity) of 2.88. The corresponding methane yields were 263 and 214 L/kg volatile solids (VS), which were 120% and 78.3% higher than for the untreated rice straw, respectively.

Although plant-based culture media enhances in vitro cultivation of rhizobacteria, studies assessing their biomass potential for large-scale applications are lacking. Here, we advance plant pellets (PPs) as a novel technology to unlock the potential of such vegan culture media for biomass production of Rhizobium leguminosarum. PP formulations were based on mixtures of Egyptian clover powder and the agro-byproducts glycerol and molasses. These mixtures were either contained or not contained in teabags during culture media preparation. Metrics of biomass included colony forming units, optical density (OD600nm), and cell dry weight (DW). Biomass comparisons between culture media based on PPs and standard yeast extract mannitol (YEM) revealed that the following PPs composition, contained in teabags, cultivated rhizobia at levels comparable to YEM: 16 g clover powder, 5% molasses, and 0.8% glycerol. This PPs composition enabled shorter generation times of rhizobia (PP: 3.83 h, YEM: 4.28 h). Strikingly, PPs mixtures supplemented with 10% molasses and not contained in teabags promoted rhizobia without apparent lag phases and produced 25% greater DW than YEM. PPs potentiate the use of dehydrated vegan feedstocks for both plant microbiota cultivation and biomass production and appear as cost- and labor-effective tools, easy to handle and store for plant-based culture media preparation.

Endophytes and rhizospheric microorganisms support invasive species’ adaptation to environmental stresses. Here, we review the impacts of endophytes, rhizospheric microbes (particularly symbiotic nitrogen-fixers), mycorrhiza and pathogens on plant invasion in arid and semi-arid areas. Endophytes and soil microorganisms either enhance nutrient acquisition for enhancing the invasive plant immune system and/or negatively affect native plants. In addition, the positive feedback between mycorrhizal fungi and invasive plants enhances the competitive ability of the aliens, providing them more opportunities for success, establishment, and dominance. The microbes and their secondary metabolites promote invasive plant species by changing soil microbial community structure and carbon biomass as well as enzyme activity, which improves soil properties and processes. The negative impact of invasive exotic plants on the associated biota and the role of allelochemicals are also discussed. It could be concluded that endophytes interact with rhizosphere microbes to promote invasive plant species in arid and semi-arid areas in a way similar to what happens in other ecosystems; the differences are in the pathways and reactions, which depend upon the prevailing abiotic factors. More interdisciplinary field experiments integrating microbial, biotechnological, and molecular approaches are needed to understand the role of symbiotic microbes in invasion biology.

Plant growth regulators are naturally biosynthesized chemicals in plants that influence physiological processes. Their synthetic analogous trigger numerous biochemical and physiological processes involved in the growth and development of plants. Nowadays, due to changing climatic scenario, numerous biotic and abiotic stresses hamper seed germination, seedling growth, and plant development leading to a decline in biological and economic yields. However, plant growth regulators (PGRs) can potentially play a fundamental role in regulating plant responses to various abiotic stresses and hence, contribute to plant adaptation under adverse environments. The major effects of abiotic stresses are growth and yield disturbance, and both these effects are directly overseen by the PGRs. Different types of PGRs such as abscisic acid (ABA), salicylic acid (SA), ethylene (ET), and jasmonates (JAs) are connected to boosting the response of plants to multiple stresses. In contrast, PGRs including cytokinins (CKs), gibberellins (GAs), auxin, and relatively novel PGRs such as strigolactones (SLs), and brassinosteroids (BRs) are involved in plant growth and development under normal and stressful environmental conditions. Besides, polyamines and nitric oxide (NO), although not considered as phytohormones, have been included in the current review due to their involvement in the regulation of several plant processes and stress responses. These PGRs are crucial for regulating stress adaptation through the modulates physiological, biochemical, and molecular processes and activation of the defense system, upregulating of transcript levels, transcription factors, metabolism genes, and stress proteins at cellular levels. The current review presents an acumen of the recent progress made on different PGRs to improve plant tolerance to abiotic stress such as heat, drought, salinity, and flood. Moreover, it highlights the research gaps on underlying mechanisms of PGRs biosynthesis under stressed conditions and their potential roles in imparting tolerance against adverse effects of suboptimal growth conditions.

The impact of climate change on biodiversity has been the subject of numerous research in recent years. The multiple elements of climate change are expected to affect all levels of biodiversity, including microorganisms. The common worldwide fungus Fusarium oxysporum colonizes plant roots as well as soil and several other substrates. It causes predominant vascular wilt disease in different strategic crops such as banana, tomato, palm, and even cotton, thereby leading to severe losses. So, a robust maximum entropy algorithm was implemented in the well-known modeling program Maxent to forecast the current and future global distribution of F. oxysporum under two representative concentration pathways (RCPs 2.6 and 8.5) for 2050 and 2070. The Maxent model was calibrated using 1885 occurrence points. The resulting models were fit with AUC and TSS values equal to 0.9 (±0.001) and 0.7, respectively. Increasing temperatures due to global warming caused differences in habitat suitability between the current and future distributions of F. oxysporum, especially in Europe. The most effective parameter of this fungus distribution was the annual mean temperature (Bio 1); the two-dimensional niche analysis indicated that the fungus has a wide precipitation range because it can live in both dry and rainy habitats as well as a range of temperatures in which it can live to certain limits. The predicted shifts should act as an alarm sign for decision makers, particularly in countries that depend on such staple crops harmed by the fungus.