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Petroleum oil pollution is a worldwide problem that results from the continuous exploration, production, and consumption of oil and its products. Petroleum hydrocarbons are produced as a result of natural or anthropogenic practices, and their common source is anthropogenic activities, which impose adverse effects on the ecosystem’s nonliving and living components including humans. Phytoremediation of petroleum hydrocarbon-polluted soils is an evolving, low-cost, and effective alternative technology to most traditional remediation methods. The objective of this study is to evaluate the phytoremediation potentiality of Vinca rosea for crude oil-contaminated soil by understanding its properties and involvement in the enhanced degradation of crude oil. The remediation potentiality was determined by evaluating the total petroleum hydrocarbon degradation percentage (TPH%) and changes in the molecular type composition of saturated and aromatic hydrocarbon fractions. TPH% was estimated gravimetrically, and changes in the molecular type composition of saturated and aromatic fractions were measured using gas chromatography and high-performance liquid chromatography, respectively. Sulfur concentration was measured using X-ray fluorescence. Cadmium and lead quantification was measured using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). The results revealed that V. rosea enhanced total petroleum hydrocarbon (TPH) degradation and altered the molecular composition of the crude oil. The saturated hydrocarbons increased and the aromatic hydrocarbons decreased. The saturated hydrocarbon fraction in the crude oil showed a wider spectrum of n-paraffin peaks than the oil extracted from unplanted and V. rosea-planted soils. Polyaromatic hydrocarbon degradation was enhanced in the presence of V. rosea, which was reflected in the increase of monoaromatic and diaromatic constituents. This was parallel to the increased sulfur levels in planted soil. The determination of sulfur and heavy metal content in plant organs indicated that V. rosea can extract and accumulate high amounts from polluted soils. The ability of V. rosea to degrade TPH and alter the composition of crude petroleum oil by decreasing the toxicity of polyaromatic hydrocarbons in soil, as well as its capability to absorb and accumulate sulfur and heavy metals, supports the use of plant species for the phytoremediation of crude oil-polluted sites.

Petroleum oil pollution is a worldwide problem that results from the continuous exploration, production, and consumption of oil and its products. Petroleum hydrocarbons are produced as a result of natural or anthropogenic practices, and their common source is anthropogenic activities, which impose adverse effects on the ecosystem’s nonliving and living components including humans. Phytoremediation of petroleum hydrocarbon-polluted soils is an evolving, low-cost, and effective alternative technology to most traditional remediation methods. The objective of this study is to evaluate the phytoremediation potentiality of Vinca rosea for crude oil-contaminated soil by understanding its properties and involvement in the enhanced degradation of crude oil. The remediation potentiality was determined by evaluating the total petroleum hydrocarbon degradation percentage (TPH%) and changes in the molecular type composition of saturated and aromatic hydrocarbon fractions. TPH% was estimated gravimetrically, and changes in the molecular type composition of saturated and aromatic fractions were measured using gas chromatography and high-performance liquid chromatography, respectively. Sulfur concentration was measured using X-ray fluorescence. Cadmium and lead quantification was measured using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). The results revealed that V. rosea enhanced total petroleum hydrocarbon (TPH) degradation and altered the molecular composition of the crude oil. The saturated hydrocarbons increased and the aromatic hydrocarbons decreased. The saturated hydrocarbon fraction in the crude oil showed a wider spectrum of n-paraffin peaks than the oil extracted from unplanted and V. rosea-planted soils. Polyaromatic hydrocarbon degradation was enhanced in the presence of V. rosea, which was reflected in the increase of monoaromatic and diaromatic constituents. This was parallel to the increased sulfur levels in planted soil. The determination of sulfur and heavy metal content in plant organs indicated that V. rosea can extract and accumulate high amounts from polluted soils. The ability of V. rosea to degrade TPH and alter the composition of crude petroleum oil by decreasing the toxicity of polyaromatic hydrocarbons in soil, as well as its capability to absorb and accumulate sulfur and heavy metals, supports the use of plant species for the phytoremediation of crude oil-polluted sites.

Fungi have unique properties and are used in many areas of agriculture and industry because they can produce different enzymes. This study aims to study the fungal diversity in peat soil from Pontian in Johor, Malaysia. The fungal isolates were described on different culture media and on a new culture medium called EVA medium and were identified using the phenotypical characteristics and molecular properties of the D1/D2 domain of the 28S large subunit ribosomal RNA (28S rRNA) and ITS (ITS1-ITS4) rDNA regions. The results revealed that 14 fungal species (15 isolates) were identified, among them, 6 were categorized as newly isolated strains and recorded in Malaysia; these include Aspergillus arenarioides EAN603, A. iizukae EAN605, Paraconiothyrium brasiliense EAN202, Parengyodontium album EAN602, Penicillium pedernalense EAN604, and Purpureocillium lilacinum EAN601. The cultural, morphological, microstructure, and molecular characteristics of these new strains have been described in this study. It was noted that the EVA medium exhibited a moderate support for fungal growth and sporulation compared to other culture media. Furthermore, the efficiency of the new medium as an enrichment medium to isolate fungi from peat soils with high ligninolytic content was discussed.

Fungi have unique properties and are used in many areas of agriculture and industry because they can produce different enzymes. This study aims to study the fungal diversity in peat soil from Pontian in Johor, Malaysia. The fungal isolates were described on different culture media and on a new culture medium called EVA medium and were identified using the phenotypical characteristics and molecular properties of the D1/D2 domain of the 28S large subunit ribosomal RNA (28S rRNA) and ITS (ITS1-ITS4) rDNA regions. The results revealed that 14 fungal species (15 isolates) were identified, among them, 6 were categorized as newly isolated strains and recorded in Malaysia; these include Aspergillus arenarioides EAN603, A. iizukae EAN605, Paraconiothyrium brasiliense EAN202, Parengyodontium album EAN602, Penicillium pedernalense EAN604, and Purpureocillium lilacinum EAN601. The cultural, morphological, microstructure, and molecular characteristics of these new strains have been described in this study. It was noted that the EVA medium exhibited a moderate support for fungal growth and sporulation compared to other culture media. Furthermore, the efficiency of the new medium as an enrichment medium to isolate fungi from peat soils with high ligninolytic content was discussed.

Land use and climate change always induce significant changes in various parameters of the hydrologic cycle (e.g., surface runoff, infiltration, evapotranspiration). The Wadi El-Assiuti downstream area in the Eastern Desert of Egypt is one of the most promising areas for development that is suffering from insufficient water availability and inadequate water quality for different purposes. The main goal of this research is to evaluate the changes in groundwater quality, land use, and climate in association with geology and flooding during three periods within the years 1997–2019 in the downstream portion of Wadi El-Assiuti in the Eastern Desert of Egypt, using spatiotemporal variation associated with groundwater hydrochemical analysis and GIS techniques. About 133 groundwater samples were collected to examine groundwater quality changes over time. Different groundwater quality indices were calculated, and the results show that TDS levels of groundwater in the study area ranged between 1080–2780 mg/L, 672–4564 mg/L, and 811–6084 mg/L, while SAR levels varied within 6.15–15.34, 1.83–28.87, and 1.43–30.57 for the years 1997, 2007, and 2019, respectively. Both RSBC and SSP values exhibited significantly increasing trends over time. KR values were within 1.36–4.06 in 1997, 0.58–14.09 in 2007, and 0.35–14.92 in 2019; MAR values were within 6.9–45.2 in 1997, 20.79–71.5 in 2007, and 17.71–75.81 in 2019; and PI values were within 60.16–83 in 1997, 45.56–101.03 in 2007, and 42.51–148.88 in 2019. Across the entire study area, ongoing land use changes increased from 1.1% in 1997 to 4.1% in 2019. Findings pointed to the significant contribution of the deep Nubian Sandstone Aquifer to the groundwater aquifer at Wadi El-Assiuti through fractures and deep faults. Given the climatic conditions from 1997–2019, these changes may have affected water quality in shallow aquifers, especially with increasing evaporation. Realizing the spatiotemporal variation of the aquifer recharge system, land use development, and climate change clearly would help in water resource management. This study revealed that flooding events, deep-seated geologic structures, and land use development associated with human activities have the highest impact on groundwater quality.

Land use and climate change always induce significant changes in various parameters of the hydrologic cycle (e.g., surface runoff, infiltration, evapotranspiration). The Wadi El-Assiuti downstream area in the Eastern Desert of Egypt is one of the most promising areas for development that is suffering from insufficient water availability and inadequate water quality for different purposes. The main goal of this research is to evaluate the changes in groundwater quality, land use, and climate in association with geology and flooding during three periods within the years 1997–2019 in the downstream portion of Wadi El-Assiuti in the Eastern Desert of Egypt, using spatiotemporal variation associated with groundwater hydrochemical analysis and GIS techniques. About 133 groundwater samples were collected to examine groundwater quality changes over time. Different groundwater quality indices were calculated, and the results show that TDS levels of groundwater in the study area ranged between 1080–2780 mg/L, 672–4564 mg/L, and 811–6084 mg/L, while SAR levels varied within 6.15–15.34, 1.83–28.87, and 1.43–30.57 for the years 1997, 2007, and 2019, respectively. Both RSBC and SSP values exhibited significantly increasing trends over time. KR values were within 1.36–4.06 in 1997, 0.58–14.09 in 2007, and 0.35–14.92 in 2019; MAR values were within 6.9–45.2 in 1997, 20.79–71.5 in 2007, and 17.71–75.81 in 2019; and PI values were within 60.16–83 in 1997, 45.56–101.03 in 2007, and 42.51–148.88 in 2019. Across the entire study area, ongoing land use changes increased from 1.1% in 1997 to 4.1% in 2019. Findings pointed to the significant contribution of the deep Nubian Sandstone Aquifer to the groundwater aquifer at Wadi El-Assiuti through fractures and deep faults. Given the climatic conditions from 1997–2019, these changes may have affected water quality in shallow aquifers, especially with increasing evaporation. Realizing the spatiotemporal variation of the aquifer recharge system, land use development, and climate change clearly would help in water resource management. This study revealed that flooding events, deep-seated geologic structures, and land use development associated with human activities have the highest impact on groundwater quality.

Institutional linkages and information flow between agricultural organizations play a critical role in addressing sustainability issues and promoting agrarian innovation. The aim of this study was to evaluate institutional relations and information between the various actors within the agricultural knowledge and information system (AKIS). The study focused on eight actors within the AKIS in Dakahlia governorate of Egypt, namely policy, extension, research, agricultural cooperatives, higher education, secondary education, credit, and the private sector. Thus, the survey sample included 11 representatives of each actor with 88 respondents. Data were collected by a standardized questionnaire distributed online. The graph theoretical technique was used for the quantitative assessment of information flow and institutional linkages established among actors. The findings indicated that agricultural extension ranked first about their real cause and effect on the rest of the system, having a value of 7.95. Two critical information pathways within the AKIS sustained innovation outcomes: (1) higher education–extension–agricultural cooperatives, (2) research–extension–agricultural cooperatives. The results also revealed that agricultural cooperatives ranked second after the extension component on the extent of supplying information to other members in the AKIS, with a value of 4.8. In contrast, the highest component received information from other components (7.6). By analyzing institutional linkages and information flow, this article gives insights to policymakers on the mechanisms that still need to be strengthened and the information gaps between actors to address the challenges of sustainable rural development.

Institutional linkages and information flow between agricultural organizations play a critical role in addressing sustainability issues and promoting agrarian innovation. The aim of this study was to evaluate institutional relations and information between the various actors within the agricultural knowledge and information system (AKIS). The study focused on eight actors within the AKIS in Dakahlia governorate of Egypt, namely policy, extension, research, agricultural cooperatives, higher education, secondary education, credit, and the private sector. Thus, the survey sample included 11 representatives of each actor with 88 respondents. Data were collected by a standardized questionnaire distributed online. The graph theoretical technique was used for the quantitative assessment of information flow and institutional linkages established among actors. The findings indicated that agricultural extension ranked first about their real cause and effect on the rest of the system, having a value of 7.95. Two critical information pathways within the AKIS sustained innovation outcomes: (1) higher education–extension–agricultural cooperatives, (2) research–extension–agricultural cooperatives. The results also revealed that agricultural cooperatives ranked second after the extension component on the extent of supplying information to other members in the AKIS, with a value of 4.8. In contrast, the highest component received information from other components (7.6). By analyzing institutional linkages and information flow, this article gives insights to policymakers on the mechanisms that still need to be strengthened and the information gaps between actors to address the challenges of sustainable rural development.