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The question of the sustainability of 1+MG infrastructure goes well beyond funding, comprising several dimensions such as scientific excellence, socio-economic impact or innovation. All these questions should be addressed over the entire life cycle - from initial planning up to decommission. In this deliverable, we compile different aspects that need to be taken into account for the future Genome Data Infrastructure (GDI) sustainability, resulting from the 1+MG initiative. Infrastructure needs Training needs Alignments with other initiatives and the EHDS Governance Investment needs Funding models Potential funding routes Socio-economic impact

"Be SURE - Be SUstainable REcommendations" The main goals and challenges for the Life Science (LS) communities in the Open Science framework are to increase reuse and sustainability of data resources, software tools, and workflows, especially in large-scale data-driven research and computational analyses. Here, we present key findings, procedures, effective measures and recommendations for generating and establishing sustainable LS resources based on the collaborative, cross-disciplinary work done within the EOSC-Life (European Open Science Cloud for Life Sciences) consortium. Bringing together 13 European LS Research Infrastructures (RIs), it has laid the foundation for an open, digital space to support biological and medical research. Using lessons learned from 27 selected projects, we describe the organisational, technical, financial and legal/ethical challenges that represent the main barriers to sustainability in the life sciences. We show how EOSC-Life provides a model for sustainable FAIR data management, including solutions for sensitive- and industry-related resources, by means of cross-disciplinary training and best practices sharing. Finally, we illustrate how data harmonisation and collaborative work facilitate interoperability of tools, data, solutions and lead to a better understanding of concepts, semantics and functionalities in the life sciences. IN PRESS EMBO Journal: https://www.embopress.org/journal/14602075 AVAILABLE SOON at : https://doi.org/10.15252/embj.2023115008  This research is mainly a product of the EOSC-Life European programme funding from the European Union's Horizon Europe research and innovation programme under grant agreement Nº824087. Complementary support was provided through EU funded project AgroServ (grant agreement Nº101058020), EU funded project BY-COVID (grant agreement Nº101046203), EU funded project DANUBIUS-IP (grant agreement Nº101079778), EU funded project EMPHASIS-GO (grant agreement Nº101079772), EU funded project FAIRplus (IMI grant agreement Nº802750), EU funded project FAIRsharing (Wellcome grant agreement Nº212930/Z/18/Z), EU funded project ISIDORe (grant agreement Nº101046133), EU funded project Precision Toxicology (grant agreement Nº965406), UKRI DASH (grant agreement NºMR/V038966/1). Special thanks to T. Biro and her radical collaboration team from Research Data Alliance who gave us great inspiration on how to lead this radical collaboration work.

The aim of this work was to determine the effect of nitrogen nutrition on biomass production of different ecotypes of T. domingensis grown hydroponically for two months. The experiment was a two-factor randomized design with five nitrogen treatments and three ecotypes, where treatments were increasing N levels (0, 20, 50, 100 and 200 mg·l-1) in the nutrient solution. Biomass production, biomass partitioning and total nitrogen content (TN) in the biomass produced by each ecotype were determined, and results were analysed by using two-way analysis of variance (ANOVA). Increasing N level resulted in a significantly increase of biomass production, where the best response was found for the ecotype from Cuenca. The N level also influenced TN; interaction between ecotype and treatment was recorded. TN was higher in the submerged biomass than in the aerial biomass. Results from this work showed that the response of hydroponically grown T. domingensis to N concentrations depends on the ecotype. Proceedings of the 27th European Biomass Conference and Exhibition, 27-30 May 2019, Lisbon, Portugal, pp. 178-181