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An aerobic granular bioreactor was operated for over 4months, treating a synthetic wastewater with a high ammonium content (100mgNL-1). The inoculum was collected from a bioreactor performing simultaneous partial nitrification and aromatic compounds biodegradation. From day-56 onwards, 2-fluorophenol (2-FP) (12.4mgL-1) was added to the feeding wastewater and the system was bioaugmented with a 2-FP degrading bacteria (Rhodococcus sp. FP1). By the end of operation, complete 2-FP biodegradation and partial nitrification were simultaneously achieved. Aerobic granules remained stable over time. During the 2-FP loading, a shift in the community structure occurred, coinciding with the improvement of 2-FP degradation. DGGE analysis did not allow to infer on the bioaugmented strain presence but pyrosequencing analysis detected Rhodococcus genus by the end of operation. Together with other potential phenolic-degraders within granules, these microorganisms were probably responsible for 2-FP degradation.

The aim of this study is to find potential utilization practice of rice straw in India from an environmental perspective. Life cycle assessment (LCA) is conducted for four most realistic utilization practices of straw including: (1) incorporation into the field as fertilizer (2) animal fodder (3) electricity (4) biogas. The results show that processing of 1 ton straw to electricity and biogas resulted in net reduction of 1471 and 1023kg CO2 eq., 15.0 and 3.4kg SO2 eq. and 6.7 and 7.1kg C2H6 eq. emissions in global warming, acidification and photochemical oxidation creation potential respectively. Electricity production from straw replaces the coal based electricity and resulted in benefits in most of the environmental impacts whereas use as an animal fodder resulted in eutrophication benefits. The burning of straw is a harmful practice of managing straw in India which can be avoided by utilizing straw for bioenergy.

The aim of this paper is to present a novel, universal, methodology for the design of low cost tubular digesters. This method improves on the established methodology by avoiding assumptions that tend to reduce the final hydraulic retention time (HRT) of digesters once installed. This work recommends designing the digester using trench cross-sectional area and proposes an optimization of the trench dimensions with respect to the angle of the walls and the relationship between the length of the biogas bell and the top width of the trench. The influence of the biogas pressure is considered. A simple geometrical analysis is presented that, by parameterization, can be applied in a wide range of situations.

This study aimed at analyzing the anaerobic co-digestion of microalgal biomass grown in wastewater and wheat straw. To this end, Biochemical Methane Potential (BMP) tests were carried out testing different substrate proportions (20-80, 50-50 and 80-20%, on a volatile solid basis). In order to improve their biodegradability, the co-digestion of both substrates was also evaluated after applying a thermo-alkaline pretreatment (10% CaO at 75°C for 24h). The highest synergies in degradation rates were observed by adding at least 50% of wheat straw. Therefore, the co-digestion of 50% microalgae - 50% wheat straw was investigated in mesophilic lab-scale reactors. The results showed that the methane yield was increased by 77% with the co-digestion as compared to microalgae mono-digestion, while the pretreatment only increased the methane yield by 15% compared to the untreated mixture. Thus, the anaerobic co-digestion of microalgae and wheat straw was successful even without applying a thermo-alkaline pretreatment.

An overall treatment process for the removal of nitrogen, methane production and obtention of valuable fertilizers from dairy manure has been investigated in laboratory scale. Solid and liquid fractions were separated by flocculation and screening. The solid fraction contained 81.6%, 84.4%, 58.6% and 85.2% of TS, VS, TKN-N and P(T) originally present in manure. Batch anaerobic digestion of this solid fraction at 50°C resulted in methane production of 29.0 L CH(4)/kg. The liquid fraction, free of suspended solids, was satisfactorily treated at 35°C in an upflow anaerobic sludge blanket reactor operating stably at an organic loading rate of 40.8 g COD/(L·d) reaching a methane production of 10.3 L CH(4)/(L·d). Accumulation of volatile fatty acids did not occur. Ammonia nitrogen concentration in the anaerobic effluent fluctuated between 850-1170 mg NH(4)(+)-N/L and was reduced to values less than 100mg NH(4)(+)-N/L by struvite precipitation.

The simultaneous removal of 4-chlorophenol (4-CP) and phenol in lab-scale sequencing batch reactors at different temperatures has been studied. Phenol feed concentration was fixed at 525 mg/L and 4-CP concentration was increased from 105 to 2100 mg/L at a constant hydraulic residence time (HRT) of 10.5 d. Complete phenol and 4-CP biodegradation was achieved during the aerobic stage working with 4-CP concentrations up to 1470 mg/L in the feed. Both 4-CP and phenol specific initial removal rates were strongly affected by 4-CP feed concentration and temperature. Only at the highest temperature tested (35 degrees C) it was possible to increase the maximum assimilative 4-CP concentration by the biological sludge up to 2100 mg/L, and a significant reduction of the ecotoxicity of the effluents was observed. 4-chlorocatechol (4-CC) was identified as the major intermediate in the aerobic cometabolic 4-CP degradation, being the ecotoxicity of that species substantially lower than that of 4-CP.

The potential of purple phototrophic bacteria (PPB) for the simultaneous treatment of piggery wastewater (PWW) and biogas upgrading was evaluated batchwise in gas-tight photobioreactors. PWW dilution was identified as a key parameter determining the efficiency of wastewater treatment and biomethane quality in PPB photobioreactors. Four times diluted PWW supported the most efficient total organic carbon (TOC) and total nitrogen removals (78% and 13%, respectively), with CH4 concentrations of 90.8%. The influence of phosphorous concentration (supplementation of 50 mg L-1 of P-PO43-) on PPB-based PWW treatment coupled to biogas upgrading was investigated. TOC removals of ≈60% and CH4 concentrations of ≈90.0% were obtained regardless of phosphorus supplementation. Finally, the use of PPB and algal-bacterial consortia supported CH4 concentrations in the upgraded biogas of 93.3% and 73.6%, respectively, which confirmed the potential PPB for biogas upgrading coupled to PWW treatment.