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Abstract The storage of heat in aquifers, also referred to as Aquifer Thermal Energy Storage (ATES), bears a high potential to bridge the seasonal gap between periods of highest thermal energy demand and supply. With storage temperatures higher than 50 °C, High-Temperature (HT) ATES is capable to facilitate the integration of (non-)renewable heat sources into complex energy systems. While the complexity of ATES technology is positively correlated to the required storage temperature, HT-ATES faces multidisciplinary challenges and risks impeding a rapid market uptake worldwide. Therefore, the aim of this study is to provide an overview and analysis of these risks of HT-ATES to facilitate global technology adoption. Risk are identified considering experiences of past HT-ATES projects and analyzed by ATES and geothermal energy experts. An online survey among 38 international experts revealed that technical risks are expected to be less critical than legal, social and organizational risks. This is confirmed by the lessons learned from past HT-ATES projects, where high heat recovery values were achieved, and technical feasibility was demonstrated. Although HT-ATES is less flexible than competing technologies such as pits or buffer tanks, the main problems encountered are attributed to a loss of the heat source and fluctuating or decreasing heating demands. Considering that a HT-ATES system has a lifetime of more than 30 years, it is crucial to develop energy concepts which take into account the conditions both for heat sources and heat sinks. Finally, a site-specific risk analysis for HT-ATES in the city of Hamburg revealed that some risks strongly depend on local boundary conditions. A project-specific risk management is therefore indispensable and should be addressed in future research and project developments.

This paper evaluates the impact of reduction of doublet well spacing, below the current West Netherlands Basin standard of 1000 to 1500 m, on the Net Present Value (NPV) and the life time of fluvial Hot Sedimentary Aquifer (HSA) doublets. First, a sensitivity analysis is used to show the possible advantage of such reduction on the NPV. The parameter value ranges are derived from West Netherlands Basin HSA doublet examples. The results indicate that a reduction of well spacing from 1400 to 1000 m could already influence NPV by up to 15%. This effect would be larger in more marginally economic HSA doublets compared to the West Netherlands Basin base case scenario. The possibility to reduce well spacing is supported by finite element production simulations, utilizing detailed facies architecture models. Furthermore, our results underline the necessity of detailed facies architecture models to assess the potential and risks of HSA doublets. This factor significantly affects doublet life time and net energy production of the doublet.

AbstractBiomass yields for several null mutants in Saccharomyces cerevisiae were successfully predicted with a metabolic network model. Energetic parameters of the model were obtained from growth data in C‐limited aerobic chemostat cultures of the corresponding wild‐type strain, which exhibited a P/O ratio of 1.46, a non‐growth‐related maintenance of 56 mmol ATP/C‐mol biomass/h, and a growth‐related requirement of 655 mmol ATP/C‐mol biomass. Biomass yields and carbon uptake rates were modeled for different mutants incapacitated in their glyoxylate cycle and their gluconeogenesis. Biomass yields were calculated for different feed ratios of glucose to ethanol, and decreases for higher ethanol fractions were correctly predicted for mutants with deletions of the malate synthase, the isocitrate lyase, or the phosphoenolpyruvate carboxykinase. The growth of the fructose‐ 1,6‐bisphosphatase deletion mutant was anticipated less accurate, but the tendency was modeled correctly. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 61–72, 2002.

The objective of this study was to assess the accuracy, spatial variation and potential value of remote sensing (RS) estimates of evapotranspiration (ET) and biomass production for irrigated sugarcane in Mpumalanga, South Africa. Weekly ET and biomass production were estimated from RS data from 2011 to 2013 using the Surface Energy Balance Algorithm for Land (SEBAL). Ground estimates of canopy interception of photosynthetically active radiation (FPAR) and aerial biomass were compared to RS estimates. ET was estimated with a surface renewal (SR) system in one field. Evaporation coefficient (Kc) values were calculated from ET and reference grass evaporation. Remote sensing FPAR and biomass estimates compared well with field measurements (R2 = 0.89 and 0.78). SEBAL ET estimates exceeded SR estimates by 5 mm/week, while full canopy Kc values for SEBAL compared better with literature values than with SR Kc values. SEBAL estimates of ET and biomass were regarded as reliable. Considerable spatial variation was observed in seasonal RS ET (1 034 ± 223 mm), biomass (45 ± 17 t/ha) and biomass water use efficiency (WUEBIO, defined as dry biomass produced per unit of ET) (4.1 ± 1.0 kg/m3). About 32% of sugarcane fields had values below economic thresholds, indicating an opportunity to increase productivity. Actual yields correlated well with WUEBIO values, suggesting that this may be used for monitoring crop performance and identifying areas that require remedial treatment.

Microbial production of fuels and chemicals from renewable carbohydrate feedstocks offers sustainable and economically attractive alternatives to their petroleum-based production. The yeast Saccharomyces cerevisiae offers many advantages as a platform cell factory for such applications. Already applied on a huge scale for bioethanol production, this yeast is easy to genetically engineer, its physiology, metabolism and genetics have been intensively studied and its robustness enables it to handle harsh industrial conditions. Introduction of novel pathways and optimization of its native cellular processes by metabolic engineering are rapidly expanding its range of cell-factory applications. Here we review recent scientific progress in metabolic engineering of S. cerevisiae for the production of bioethanol, advanced biofuels, and chemicals.

AbstractChemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B‐group vitamins, which are either enzyme cofactors or precursors for their synthesis. Despite the widespread use of CDMY in fundamental and applied yeast research, the relation of their design and composition to the actual vitamin requirements of yeasts has not been subjected to critical review since their first development in the 1940s. Vitamins are formally defined as essential organic molecules that cannot be synthesized by an organism. In yeast physiology, use of the term “vitamin” is primarily based on essentiality for humans, but the genome of the Saccharomyces cerevisiae reference strain S288C harbours most of the structural genes required for synthesis of the vitamins included in popular CDMY. Here, we review the biochemistry and genetics of the biosynthesis of these compounds by S. cerevisiae and, based on a comparative genomics analysis, assess the diversity within the Saccharomyces genus with respect to vitamin prototrophy.

In this paper we present results of a global resource assessment for geothermal energy within deep aquifers for direct heat utilization. Greenhouse heating, spatial heating, and spatial cooling are considered in this assessment. We derive subsurface temperatures from geophysical data and apply a volumetric heat-in-place method to improve current global geothermal resource base estimates for direct heat applications. The amount of thermal energy stored within aquifers depends on the Earth's heat flow, aquifer volume, and thermal properties. We assess the thermal energy available by estimating subsurface temperatures up to a depth of three kilometer depending on aquifer thickness. The distribution of geothermal resources is displayed in a series of maps and the depth of the minimum production temperature is used as an indicator of performance and technical feasibility. Suitable aquifers underlay 16% of the Earth's land surface and store an estimated 4·105 to 5·106 EJ that could theoretically be used for direct heat applications. Even with a conservative recovery factor of 1% and an assumed lifetime of 30 years, the annual recoverable geothermal energy is in the same order as the world final energy consumption of 363.5 EJ yr−1. Although the amount of geothermal energy stored in aquifers is vast, geothermal direct heat applications are currently underdeveloped with less than one thousandth of their technical potential used.