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The Galapagos Islands have been declared a World Heritage site due to their unique biodiversity, which makes them a living museum and a natural laboratory for humankind. However, to fulfill the energy needs of its habitants and foreign visitors, the islands have depended on fossil fuel energies that have produced levels of lead and chemical agents that are affecting the islands’ air quality, flora, and fauna. Therefore, zero-carbon initiatives have been created to protect the islands, wherein solar and wind power plants have been studied as reliable alternatives. In this way, Geographical Information Systems based on Multicriteria Decision Methods constitute a methodology that minimizes the destruction and disturbance of nature in order to assess the best location for the implementation of these alternative energy sources. Therefore, by exploring the geographical information along with the Analytical Hierarchical Processes and the Ordered Weighted Average methods, it was possible to identify the potential for solar power plants of 10 MW on each island; likewise, for wind power plants, it was found that the islands possess implementation potential that has been analyzed in the field, showing that the best location is on Baltra Island, but is not limited to it.

The search for energy efficient construction solutions is still pending in the agro-food industry, in which a large amount of energy is often consumed unnecessarily when storing products. The main objective of this research is to promote high energy efficiency built environments, which aim to reduce energy consumption in this sector. We analyze the suitability of using the thermal inertia of the ground to provide an adequate environment for the storage and conservation of agro-food products. This research compares different construction solutions based on the use of ground thermal properties, analyzing their effectiveness to decrease annual outdoor variations and provide adequate indoor conditions. The analysis undertaken is based on over five million pieces of data, obtained from an uninterrupted four year monitoring process of various constructions with different levels of thermal mass, ranging from high volume constructions to others lacking this resource. It has been proven that constructive solutions based on the use of ground thermal inertia are more effective than other solutions when reducing the effects of outdoor conditions, even when these have air conditioning systems. It is possible to reach optimal conditions to preserve agro-food products such as wine, with a good design and an adequate amount of terrain, without having to use air conditioning systems. The results of this investigation could be of great use to the agro-food industry, becoming a reference when it comes to the design of energy efficient constructions.

Lignocellulosic biomass pyrolysis could be an economically feasible option for forest management as it reduces the need to burn litter and helps in fire prevention thus avoiding the release of carbon dioxide and other greenhouse gases into the atmosphere. This study characterises wood vinegar (WV) obtained via a continuous fast pyrolysis process in terms of its composition, ageing and herbicidal properties. The aqueous WV fraction had a moisture content of 84% in weight and contained more than 200 compounds. Acetic acid, hydroxyacetaldehyde and hydroxyacetone were the major components. No significant differences were found in WV composition according to the starting material (poplar, pine, pruning litter, forest waste). No residual aromatic polycyclic compounds that could be harmful to the environment were detected. In a series of climate-controlled glass chamber experiments, the WV proved to be as effective an inhibitor of seed germination and seedling growth as a contact herbicide acting against weeds, especially through aerial contact. Sprayed WV concentrations of 50, 75 and 100 vol. % were effective against all plant species tested. This product could therefore be of commercial interest and help make biomass pyrolysis economically viable, once environmental exposure limits and the safe application for agricultural and urban use of this product have been established.

Cold stress in sheep is usually overlooked, even though the animals’ welfare and productivity are affected by low temperatures. The aim of this research was to find out if and to what extent the temperature inside a sheep barn could be maintained within the range of the thermoneutral zone during winter, primarily to increase feed conversion and to reduce GHG emissions. For this reason, an automation system was installed at a sheep barn in northern Greece, and heat losses from the building were calculated. The biogas potential of the sheep barn waste was examined in the laboratory via the BMP method. The results showed that the installation of an automation system together with a hypothetical biogas heating system could maintain the barn’s temperature in the range of a sheep’s thermoneutral zone during winter for the 94% of the scenarios examined if the total energy of the biogas was utilized, while heating energy that was instantly and continuously used succeeded in 48% of the investigated cases. The surplus of energy produced by biogas could potentially raise the water temperature that animals drink up to 2.9 °C. The absence of cold stress decreases the dry matter intake and the CH4 produced by ruminal fermentation. Moreover, lower GHG emissions are achieved as waste is treated through anaerobic digestion, which would likely be released into the environment if left untreated.

Wheat straw is an abundant source of lignocellulosic biomass that is generally not utilized for biofuel production, nor for other uses. Recent EU renewable energy directive fosters bioethanol production through lignocellulosic sugars fermentation, but the cost of this process is an issue that often depends on biomass characteristics. Lignin is a class of three-dimensional polymers providing structural integrity of plant tissues. Its complex structure, together with hemicelluloses and uronic acids content, could affect the ability of hydrolyzing biomass to fermentable sugars. To get insights into this variation, a set of 10 durum wheat genotypes was analyzed to determine variation of straw digestibility to fermentable sugars. The results showed that the lignin content was the major factor determining the recalcitrance to enzymatic process. The analysis of Spearman’s correlation indicated that the sugar released after enzymatic hydrolysis had a negative connection with the lignin content, while it was positively correlated with the culm length. The possible role of other cell wall components, such as arabinose and uronic acids, was also discussed. This work aimed at analyzing the diversity of lignocellulosic digestibility to fermentable sugars of wheat straw in a small germplasm collection. Some of the selected genotypes were characterized by high sugars digestibility and high grain yield, characteristics that could make biorefining of wheat straw profitable.

The climate change that plagues the world is causing extended periods of water shortage. This situation is forcing farmers in the region of Murcia in Spain to modernize their irrigation systems to optimize use of the scarce water they have and seek a circular water economy using the recovered water. Moreover, an associated problem is the need for energy that these facilities require in order to pressurize the required water. The use of photovoltaic generation contributes to the reduction of greenhouse gas (GHG) emissions. Food produced in this region tends to have guaranteed markets in Europe and, geographically, due to the high quality of phytosanitary controls and traceability during their marketing, their optimal cultivation, and selection and labelling is verified, specifying valuable information such as: collection date, origin, the use of organic fertilizers among others. To maintain market access, it is important to continue implementing other environmental improvements, i.e., reductions in either hydro or carbon footprints. Previous studies have failed to include the prospect of environmental use of isolated facilities to replace existing consumption, seeking the monetarization of the facility as well as prioritizing the reduction of GHG. Previous studies have failed to include the perspective of environmental use of isolated photovoltaic installations, based on existing consumption, thus, going beyond the monetarization of the facility, to prioritize the reduction of GHG applied in practice by environmentally sensitized farmers. This study was conducted in an existing facility with great technical complexity and three different sources of water supply, over 1500 plots and an altitude range in plots and reservoirs of more than 400 m.

Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.