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Ambitious international targets are being developed to protect and restore biodiversity under the Convention on Biological Diversity's post-2020 Global Biodiversity Framework and the European Union's Green Deal. Yet, the land system consequences of meeting such targets are unclear, as multiple pathways may be able to deliver on the set targets. This paper introduces a novel scenario approach assessing the plural implementations of these targets. The Nature Futures Framework (NFF) developed by the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services aims to illustrate the different, positive ways in which society can value nature. It therefore offers a lens through which the spatial implementation of sustainability targets may be envisioned. We used CLUMondo, a spatially explicit model, to simulate plural land system scenarios for Europe for 2050. The model builds on current land system representations of Europe and explores how and where sustainability targets can be implemented under projected population trends and commodity demands. We created three different scenarios in which the sustainability targets are met, each representing an alternative, normative view on nature as represented by the NFF, favoring land systems providing strong climate regulation (Nature for Society), species conservation (Nature for Nature), or agricultural heritage features (Nature as Culture). Our results show that, irrespective of the NFF view, meeting sustainability targets will require European land systems to drastically change, as natural grasslands and forests are forecast to expand while productive areas are projected to undergo a dual intensification and diversification trajectory. Despite each NFF perspective showcasing a similar direction of change, 20% of Europe's land area will differ based on the adopted NFF perspective, with hotspots of disagreement identified in eastern and western Europe. These simulations go beyond existing scenario approaches by not only depicting broad societal developments for Europe, but also by quantifying the land system synergies and trade-offs associated with alternative, archetypal, interpretations and values of how nature may be managed for sustainability. This quantification exemplifies a means towards constructive dialogue, on the one hand by acknowledging areas of contention, and bringing such issues to the fore, and on the other by highlighting points of convergence in a vision for a sustainable Europe.

Lipase‐catalyzed transesterification of triglycerides and alcohols to obtain biodiesel is an environmentally friendly and sustainable route for fuels production since, besides proceeding in mild reaction conditions, it allows for the use of low‐cost feedstocks that contain water and free fatty acids, for example non‐edible oils and waste oils. This review article reports recent advances in the field and focus in particular on a major issue in the enzymatic process, the inactivation of most lipases caused by methanol, the preferred acyl acceptor used for alcoholysis. The recent results about immobilization of enzymes on nano‐materials and the use of whole‐cell biocatalysts, as well as the use of cell‐surface display technologies and metabolic engineering strategies for microbial production of biodiesel are described. It is discussed also insight into the effects of methanol on lipases obtained by modeling approaches and report on studies aimed at mining novel alcohol stable enzymes or at improving robustness in existing ones by protein engineering.

The stark observation of the co-existence of undernourishment, nutrient deficiencies and overweight and obesity, the triple burden of malnutrition, is inviting us to reconsider health and nutrition as the primary goal and final endpoint of food systems. Agriculture and the food industry have made remarkable advances in the past decades. However, their development has not entirely fulfilled health and nutritional needs, and moreover, they have generated substantial collateral losses in agricultural biodiversity. Simultaneously, several regions are experiencing unprecedented weather events caused by climate change and habitat depletion, in turn putting at risk global food and nutrition security. This coincidence of food crises with increasing environmental degradation suggests an urgent need for novel analyses and new paradigms. The sustainable diets concept proposes a research and policy agenda that strives towards a sustainable use of human and natural resources for food and nutrition security, highlighting the preeminent role of consumers in defining sustainable options and the importance of biodiversity in nutrition. Food systems act as complex social–ecological systems, involving multiple interactions between human and natural components. Nutritional patterns and environment structure are interconnected in a mutual dynamic of changes. The systemic nature of these interactions calls for multidimensional approaches and integrated assessment and simulation tools to guide change. This paper proposes a review and conceptual modelling framework that articulate the synergies and tradeoffs between dietary diversity, widely recognised as key for healthy diets, and agricultural biodiversity and associated ecosystem functions, crucial resilience factors to climate and global changes.

To contribute to the debate on climate-proof urban regeneration, the illustrated study seeks to understand how the provision of new multiscalar, multidimensional, and integrated planning tools based on sustainable and resilient strategies can guarantee high levels of urban, environmental, and energy efficiency and quality, as well as circularity of resources, counteracting the effects deriving from climate change. Starting from some regulatory and design references that integrate a new ecologically oriented city model into the planning of urban projects, the contribution identifies in the construction of green and blue infrastructures (GI) new design metaphors capable of improving biodiversity; favoring ecological and energy transition; restoring the quality of the air, water, and soil environmental matrices with natural solutions; and making cities truly inclusive, sustainable, and resilient. The methodology adopted for the design of the “Acilia–Madonnetta” Urban and Metropolitan Centrality in the Municipality of Rome simulates a planning process for part of the X Municipality by applying an iterative and interscalar logic, an articulation of levels and phases with the aim of prefiguring the construction of a GI characterized by new ecological-environmental and functional endowments strictly related to the promotion of an efficient, smart, and green city. The contribution highlights the potential and limits of the proposed experimentation, relating both to the quality and innovation of design solutions and possible evolutionary lines and to the lack of clear institutional governance that is limiting the implementation of projects.

Biogas is a renewable energy resource produced during the anaerobic digestion of various organic substrates. A wide community of microorganisms is involved, including methanogens. These Archaea are the biologic key to the process because they accomplish the methane-forming reaction. Despite its crucial role, the microbiome inside the digester is poorly understood. The aim of this work is to develop bioindicators of efficiency for the anaerobic process through the quantification and characterisation of the methanogens and sulphate-reducing bacteria. From a full-scale digester fed with organic wastes, 31 samples were collected. Temperature, pH, acidity, alkalinity and biogas quantity and quality were monitored over time. The methanogens were detected from the samples both in total and as belonging to different taxa units. These evaluations, by real-time quantitative PCR (RT-qPCR) methods, produced valuable results for Methanosarcina, Methanosaeta, Methanocorpusculaceae and sulphate-reducing bacteria. Methanosarcina was the most abundant family, followed by Methanocorpusculaceae and then Methanosaeta. The methanogen taxa are significantly and directly correlated with each other (p < 0.05). Methanosaeta and Methanocorpusculaceae are present in significantly different amounts at different temperatures. While Methanosaeta levels also change when the organic load increases (t test, p < 0.05), Methanosarcina is more tolerant, and its levels are quite constant. Methanosarcina and Methanosaeta are proposed to be bioindicators of the stability of the process (the first) and of susceptibility (the second) to detect early sufferance conditions in the digester. These methods will be useful in the control and optimisation of an eco-friendly waste-to-energy system.

Meat replacers could play a role in achieving more plant-based diets, but their current consumption is limited. The present modelling study aimed to explore the nutritional and greenhouse gas emissions impacts of meat replacers. Using dietary surveys from Denmark, Czech Republic, Italy and France (~6500 adults), we composed alternative diets in which all the meat in the observed diet (in grams) was substituted by similar use meat replacers (with and without fortification). Starting from the observed diets and meat-replacement diets, diets with improved adherence to food-based dietary guidelines (FBDGs) were modelled using Data Envelopment Analysis. These improved diets were then further optimised for dietary preferences (MaxP, diet similarity index), nutrient quality (MaxH, Nutrient Rich Diet score, NRD15.3) or diet-related greenhouse gas emissions (GHGE) (MaxS, CO2 equivalents). In all optimised modelled diets, the total amount of meat was lower than in the observed diets, i.e., 30% lower in the MaxP, 50% lower in the MaxH, and 75% lower in the MaxS diets. In the MaxP diet, NRD15.3 was ~6% higher, GHGE was ~9% lower, and ~83% of food intake remained similar. In the MaxH diet, NRD15.3 was ~17% higher, GHGE was ~15% lower, and ~66% of food intake remained similar. In the MaxS diet, NRD15.3 was ~9% higher, GHGE was ~33% lower, and ~65% of food intake remained similar. When using fortified meat replacers, for all modelled diets, the diet similarity was on average 2% lower and the GHGE reduction was on average 3% higher as compared with the same scenarios without fortification. This analysis showed that meat replacers, provided their preference is similar to meat, can provide benefits for GHGE, without necessarily compromising nutrient quality.

The sustainability of fishery is a global challenge due to overfishing and reduced stocks all over the world; one of the leading factors of this threat is fish loss/waste. As a contribution to the global efforts towards a sustainable world, this review addresses the topic from different sides and proposes an overview of biorefinery approaches by discussing bioactive compounds that could be produced from fish loss (nitrogen compounds, lipids, minerals and pigments, and fish-based compounds such as chitosan). The second part of this review reports on the possibility of using loss or unwanted fish to design products for human consumption or for animal feeding, with a focus on economic criteria, consumers’ segmentation, and some examples of products. The final focus is on Food and Agriculture Organization FAO guidelines as a roadmap for the future with respect to solving this threat by addressing the problem from different sides (technology, skills, market, policy, social and gender equity, and infrastructures).