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description Publicationkeyboard_double_arrow_right Article , Journal 2010 Australia, FrancePublisher:Elsevier BV Funded by:ARC | Advanced Stability Sensor..., EC | ANAMIXARC| Advanced Stability Sensor for Anaerobic Digestion Processes ,EC| ANAMIXAuthors:Bastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
+1 AuthorsSteyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
Steyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBatstone, Damien J.;
Batstone, Damien J.
Batstone, Damien J. in OpenAIREpmid: 21111163
Membrane inlet mass spectrometry (MIMS) is useful for on-line monitoring of fermentation processes. However, readings are affected by the complex and dynamic matrix in which biological processes occur, making MIMS calibration a challenge. In this work, two calibration strategies were evaluated for measurement of typical products of acidogenic fermentation, i.e., ethanol, H(2), and CO(2) in the liquid phase, and H(2) and CO(2) in the gas phase: (1) "standard calibration", which was performed independent of fermentation experiments with sterile standards in water with a N(2) headspace, and (2) "in-process calibration" whereby fermentation was monitored concurrent with off-line analysis. Fermentation was operated in batch and continuous modes. In-process calibration was shown to be most effective for measurements of H(2) and CO(2) in both gas and liquid phases; standard calibration gave erroneous results. In the gas phase, this was due to a lower sensitivity during experiments compared to the independent standard calibration, believed to be caused by formation of a liquid film on the surface of the probe. In the liquid phase, moving from the standard calibration environment to the fermentation caused the linear relationship between the H(2) concentration and MIMS signal to change in intercept, and the relationship for CO(2) to change in slope, possibly due to dissolved ions, and related non-ideality. For ethanol, standard calibration results were fairly consistent with in-process calibration results. The main limitation with in-process calibration is the potential for a lack of variability in target concentration. This could be addressed by spiking the targeted compound at the end of the experiment. Regardless, MIMS is an ideal instrument for analysing fermentation experiments, due to its ability to measure targeted compounds semi-continuously, and due to a lack of drift over long periods.
Hyper Article en Lig... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)Institut National de la Recherche Agronomique: ProdINRAArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.talanta.2010.09.034&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Hyper Article en Lig... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)Institut National de la Recherche Agronomique: ProdINRAArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.talanta.2010.09.034&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010 Australia, FrancePublisher:Elsevier BV Funded by:ARC | Advanced Stability Sensor..., EC | ANAMIXARC| Advanced Stability Sensor for Anaerobic Digestion Processes ,EC| ANAMIXAuthors:Bastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
+1 AuthorsSteyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
Steyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBatstone, Damien J.;
Batstone, Damien J.
Batstone, Damien J. in OpenAIREpmid: 21111163
Membrane inlet mass spectrometry (MIMS) is useful for on-line monitoring of fermentation processes. However, readings are affected by the complex and dynamic matrix in which biological processes occur, making MIMS calibration a challenge. In this work, two calibration strategies were evaluated for measurement of typical products of acidogenic fermentation, i.e., ethanol, H(2), and CO(2) in the liquid phase, and H(2) and CO(2) in the gas phase: (1) "standard calibration", which was performed independent of fermentation experiments with sterile standards in water with a N(2) headspace, and (2) "in-process calibration" whereby fermentation was monitored concurrent with off-line analysis. Fermentation was operated in batch and continuous modes. In-process calibration was shown to be most effective for measurements of H(2) and CO(2) in both gas and liquid phases; standard calibration gave erroneous results. In the gas phase, this was due to a lower sensitivity during experiments compared to the independent standard calibration, believed to be caused by formation of a liquid film on the surface of the probe. In the liquid phase, moving from the standard calibration environment to the fermentation caused the linear relationship between the H(2) concentration and MIMS signal to change in intercept, and the relationship for CO(2) to change in slope, possibly due to dissolved ions, and related non-ideality. For ethanol, standard calibration results were fairly consistent with in-process calibration results. The main limitation with in-process calibration is the potential for a lack of variability in target concentration. This could be addressed by spiking the targeted compound at the end of the experiment. Regardless, MIMS is an ideal instrument for analysing fermentation experiments, due to its ability to measure targeted compounds semi-continuously, and due to a lack of drift over long periods.
Hyper Article en Lig... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)Institut National de la Recherche Agronomique: ProdINRAArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.talanta.2010.09.034&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Hyper Article en Lig... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)Institut National de la Recherche Agronomique: ProdINRAArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.talanta.2010.09.034&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 Australia, FrancePublisher:Elsevier BV Authors:Bastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida; Zeng, Raymond J.;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREBernet, Nicolas;
+3 AuthorsBernet, Nicolas
Bernet, Nicolas in OpenAIREBastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida; Zeng, Raymond J.;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREBernet, Nicolas;
Bernet, Nicolas
Bernet, Nicolas in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
Steyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBatstone, Damien John;
Batstone, Damien John
Batstone, Damien John in OpenAIREpmid: 22342590
Acidogenic fermentation is an anaerobic process of double purpose, while treating organic residues it produces chemical compounds, such as hydrogen, ethanol and organic acids. Therefore, acidogenic fermentation arises as an attractive biotechnology process towards the biorefinery concept. Moreover, this process does not need sterile operating conditions and works under a wide range of pH. Changes of operating conditions produce metabolic shifts, inducing variability on acidogenic product yield. To induce those changes, experiments, based on reactor headspace N(2)-flushing (gas phase), were designed. A major result was the hydrogen yield increase from 1 to 3.25±0.4 ( [Formula: see text] ) at pH 4.5 and N(2)-flushing of 58.4 (L·d(-1)). This yield is close to the theoretical acidogenic value (4 [Formula: see text] ). The mechanisms that explain this increase on hydrogen yield shifts are related to the thermodynamics of three metabolic reactions: lactate hydrogenase, NADH hydrogenase and homoacetogenesis, which are affected by the low hydrogen partial pressures.
Hyper Article en Lig... arrow_drop_down Institut National de la Recherche Agronomique: ProdINRAArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2012.01.122&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu53 citations 53 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Hyper Article en Lig... arrow_drop_down Institut National de la Recherche Agronomique: ProdINRAArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2012.01.122&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 Australia, FrancePublisher:Elsevier BV Authors:Bastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida; Zeng, Raymond J.;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREBernet, Nicolas;
+3 AuthorsBernet, Nicolas
Bernet, Nicolas in OpenAIREBastidas Oyanedel, Juan Rodrigo;
Mohd-Zaki, Zuhaida; Zeng, Raymond J.;Bastidas Oyanedel, Juan Rodrigo
Bastidas Oyanedel, Juan Rodrigo in OpenAIREBernet, Nicolas;
Bernet, Nicolas
Bernet, Nicolas in OpenAIREPratt, Steven;
Pratt, Steven
Pratt, Steven in OpenAIRESteyer, Jean-Philippe;
Steyer, Jean-Philippe
Steyer, Jean-Philippe in OpenAIREBatstone, Damien John;
Batstone, Damien John
Batstone, Damien John in OpenAIREpmid: 22342590
Acidogenic fermentation is an anaerobic process of double purpose, while treating organic residues it produces chemical compounds, such as hydrogen, ethanol and organic acids. Therefore, acidogenic fermentation arises as an attractive biotechnology process towards the biorefinery concept. Moreover, this process does not need sterile operating conditions and works under a wide range of pH. Changes of operating conditions produce metabolic shifts, inducing variability on acidogenic product yield. To induce those changes, experiments, based on reactor headspace N(2)-flushing (gas phase), were designed. A major result was the hydrogen yield increase from 1 to 3.25±0.4 ( [Formula: see text] ) at pH 4.5 and N(2)-flushing of 58.4 (L·d(-1)). This yield is close to the theoretical acidogenic value (4 [Formula: see text] ). The mechanisms that explain this increase on hydrogen yield shifts are related to the thermodynamics of three metabolic reactions: lactate hydrogenase, NADH hydrogenase and homoacetogenesis, which are affected by the low hydrogen partial pressures.
Hyper Article en Lig... arrow_drop_down Institut National de la Recherche Agronomique: ProdINRAArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2012.01.122&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu53 citations 53 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Hyper Article en Lig... arrow_drop_down Institut National de la Recherche Agronomique: ProdINRAArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2012Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.biortech.2012.01.122&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2016 AustraliaPublisher:Elsevier BV Authors:Yang Lu;
Alan Werker; Alan Werker;Steven Pratt;
+4 AuthorsSteven Pratt
Steven Pratt in OpenAIREYang Lu;
Alan Werker; Alan Werker;Steven Pratt;
Steven Pratt
Steven Pratt in OpenAIRELiliana Montano-Herrera;
Liliana Montano-Herrera
Liliana Montano-Herrera in OpenAIREBronwyn Laycock;
Om Murugan Janarthanan; Monica V. Arcos-Hernandez;Bronwyn Laycock
Bronwyn Laycock in OpenAIREpmid: 26257140
The use of mixed microbial cultures for the production of polyhydroxyalkanoates (PHAs) is emerging as a viable technology. In this study, 16S rRNA gene amplicon pyrosequencing was used to analyse fluctuations in populations over a 63-day period within a PHA-storing mixed microbial community enriched on fermented whey permeate. This community was dominated by the genera Flavisolibacter and Zoogloea as well as an unidentified organism belonging to the phylum Bacteroidetes. The population was observed to cycle through an increase in Zoogloea followed by a return to a community composition similar to the initial one (highly enriched in Flavisolibacter). It was found that the PHA accumulation capacity of the community was robust to population flux during enrichment and even PHA accumulation, with final polymer composition dependent on the overall proportion of acetic to propionic acids in the feed. This community adaptation suggests that mixed culture PHA production is a robust process.
New Biotechnology arrow_drop_down Queensland University of Technology: QUT ePrintsArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2015.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 41 citations 41 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert New Biotechnology arrow_drop_down Queensland University of Technology: QUT ePrintsArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2015.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2016 AustraliaPublisher:Elsevier BV Authors:Yang Lu;
Alan Werker; Alan Werker;Steven Pratt;
+4 AuthorsSteven Pratt
Steven Pratt in OpenAIREYang Lu;
Alan Werker; Alan Werker;Steven Pratt;
Steven Pratt
Steven Pratt in OpenAIRELiliana Montano-Herrera;
Liliana Montano-Herrera
Liliana Montano-Herrera in OpenAIREBronwyn Laycock;
Om Murugan Janarthanan; Monica V. Arcos-Hernandez;Bronwyn Laycock
Bronwyn Laycock in OpenAIREpmid: 26257140
The use of mixed microbial cultures for the production of polyhydroxyalkanoates (PHAs) is emerging as a viable technology. In this study, 16S rRNA gene amplicon pyrosequencing was used to analyse fluctuations in populations over a 63-day period within a PHA-storing mixed microbial community enriched on fermented whey permeate. This community was dominated by the genera Flavisolibacter and Zoogloea as well as an unidentified organism belonging to the phylum Bacteroidetes. The population was observed to cycle through an increase in Zoogloea followed by a return to a community composition similar to the initial one (highly enriched in Flavisolibacter). It was found that the PHA accumulation capacity of the community was robust to population flux during enrichment and even PHA accumulation, with final polymer composition dependent on the overall proportion of acetic to propionic acids in the feed. This community adaptation suggests that mixed culture PHA production is a robust process.
New Biotechnology arrow_drop_down Queensland University of Technology: QUT ePrintsArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2015.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 41 citations 41 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert New Biotechnology arrow_drop_down Queensland University of Technology: QUT ePrintsArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2016Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.nbt.2015.07.007&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu