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Microbe-assisted phytoremediation is emerging as one of the most effective means by which plants and their associated rhizosphere microbes degrade organic contaminants in soils. A pot study was conducted to examine the effects of inoculation with Rhizobium meliloti on phytoremediation by alfalfa grown for 90 days in an agricultural soil contaminated with weathered polycyclic aromatic hydrocarbons (PAHs). Planting with uninoculated alfalfa (P) and alfalfa inoculated with R. meliloti (PR) significantly lowered the initial soil PAH concentrations by 37.2 and 51.4% respectively compared with unplanted control soil. Inoculation with R. meliloti significantly increased the counts of culturable PAH-degrading bacteria, soil microbial activity and the carbon utilization ability of the soil microbial community. The results suggest that the symbiotic association between alfalfa and Rhizobium can stimulate the rhizosphere microflora to degrade PAHs and its application may be a promising bioremediation strategy for aged PAH-contaminated soils.

Results-based financing (RBF) programmes in the clean cooking sector have gained increasing donor interest over the last decade. Although the risks and advantages of RBF have been discussed quite extensively for other sectors, especially health services, there is limited research-documented experience of its application to clean cooking. Due to the sheer scale of the important transition from ‘dirty’ to clean cooking for the 4 billion people who lack access, especially in the Global South, efficient and performance-proven solutions are urgently required. This paper, undertaken as part of the work of the UKAid-funded Modern Energy Cooking Services (MECS) programme, aims to close an important research gap by reviewing evidence-based support mechanisms and documenting essential experiences from previous and ongoing RBF programmes in the clean cooking and other sectors. On this basis, the paper derives key strategic implications and learning lessons for the global scaling of RBF programmes and finds that qualitative key performance indicators such as consumer acceptance as well as longer-term monitoring are critical long-term success factors for RBF to ensure the continued uptake and use of clean cooking solutions (CCS), however securing the inclusion of these indicators within programmes remains challenging. Finally, by discussing the opportunities for the evolution of RBF into broader impact funding programmes and the integration of energy access and clean cooking strategies through multi-sector approaches, the paper illustrates potential steps to enhance the impact of RBF in this sector in the future.

This work has been developed under the AGROinLOG Project, “Demonstration of innovative integrated biomass logistics centres for the Agro-industry sector in Europe”. An Integrated Biomass Logistics Center (IBLC), is based on the introduction of new production chains into existing agro-industries by using new biomass feedstock. The AGROinLOG Project has dedicated great attention to investigate the potential of cereal chaff as a valuable resource.Chaff is the fine fraction of the thrashing residues, not usually collected. Chaff is made up of glumes, seed husks, rachis and the tinner part of the cereal stems, whole and cracked kernels, as well as weed seeds.Currently there are several mechanical solutions available on the market for chaff recovery, and others are still at prototype stage, but theyare not so common and very often unknown to the farmers.So far, the literature reportsfew cases of chaff collection with the specific purpose of weed seeds removal, but it still lacks specificexperiments on these machinesintentionally used for biomass collection.For this reason, during the Project AGROinLOG a series of large field tests were performed using an independent scientific approach with different kind of chaff harvesting technologiesin France, Sweden and Italy from 2017 to 2019.The present study collects the results of these activities with the aim to fill that gap and provide deeper understanding in the possibility to enhance the current cereal harvesting method, in order to improve the quantity of biomass collected by including the chaff. Proceedings of the 29th European Biomass Conference and Exhibition, 26-29 April 2021, Online, pp. 62-68

AbstractAimAgriculture is one of the greatest pressures on biodiversity. Regional studies have shown that the presence of natural habitat and landscape heterogeneity are beneficial for biodiversity in agriculture, but it remains unclear whether their importance varies geographically. Here, we use local biodiversity data to determine which local and landscape variables are most associated with biodiversity patterns and whether their association varies between tropical and non‐tropical regions.LocationGlobal terrestrial area in forest biomes.Major taxa studiedMore than 21,000 species of vertebrates, invertebrates, plants and other taxa.MethodsWe used generalized linear mixed‐effects models to analyse the relationships between either community total abundance or species richness (derived from the PREDICTS database) and a number of site‐level (predominant land use and land‐use intensity) and landscape‐level variables (distance to forest, the percentage of natural habitat in the surrounding landscape, landscape homogeneity, the number of land‐cover types in the landscape, and total fertilizer application). We compared the associations of these variables with biodiversity in tropical and non‐tropical regions.ResultsIn most cases, changes in biodiversity associated with landscape‐level variables were greater than those associated with local land use and land‐use intensity. Increased natural habitat availability was associated with the most consistent increases in biodiversity. Landscape homogeneity was also important but showed different directions of biodiversity change between regions. Associations with fertilizer application or the number of land‐cover types were generally weaker, although still of greater magnitude than for the local land‐use measures.Main conclusionsOur results highlight similarities and differences in the association of local‐ and landscape‐scale variables with local biodiversity in tropical and non‐tropical regions. Landscape natural habitat availability had a consistent positive association with biodiversity, highlighting the key role of landscape management in the maintenance of biodiversity in croplands. Landscape‐scale variables were almost always associated with greater changes in biodiversity than the local‐scale measures.

This article reports upon research on vegan transition, which I bring into dialogue with Sara Ahmed’s figure of the killjoy. Ahmed’s work on affect and the feminist killjoy is found to be apt for considering contemporary vegans and their transgression of normative scripts of happiness and commensality in a dominant meat and dairy consuming culture. The decentring of joy and happiness is also found to be integral to the critical deconstructive work of the vegan killjoy. Ahmed’s ideas further complement the frame of practice theory that I draw upon to understand the process of transition especially in the sense of opposing the meanings of dominant practices. Although food and veganism are not commented upon by Ahmed, the vegan subject constitutes, I argue, a potent further example of what she terms an “affect alien” who must willfully struggle against a dominant affective order and community. Drawing upon interviews with 40 vegans based in the UK, I illustrate examples of contestation and negotiation by vegans and those close to them. The article finds in the figure of the killjoy not only a frame by which to partly understand the negotiation of relationships between vegans and non-vegans but also an opportunity for further intersectional labour between veganism and feminism.

Cassava utilisation in Malawi is negatively affected by rapid deterioration of fresh roots, primarily caused by postharvest physiological deterioration (PPD). A study was conducted to assess farmers’ knowledge and approaches used to minimize losses from PPD. Multi-stage sampling was used to identify districts, Extension Planning Areas (EPA’s) and farmers. Data were collected from 519 farmers using a structured questionnaire. Results revealed that PPD (74.0%) was the major post-harvest constraint followed by pests and diseases (62.1%). Farmers had varying knowledge levels on signs and causes of PPD. They were knowledgeable on PPD signs with 91.5% ably identifying PPD through change of pulp colour. The farmers also had moderate knowledge on causes of PPD, citing high temperature (57.6%) and over-staying of roots (56.2%) as main causes of PPD. Key methods for preventing PPD are: storage (43.0%) and piece-meal harvesting (40.4%). Only 2.6% of the farmers exploited varietal difference in dealing with PPD as some varieties (Sauti, Mpuma, Ching’amba, and Kalasa) take three to five days before showing PPD signs. Farmers’ knowledge levels and PPD preventive methods could be strengthened through: provision of training on post-harvest handling, improvement in storage and processing technologies; and application of advanced breeding techniques to exploit genetic variation in cassava germplasm.

Differences in bacterial community composition (BCC) between bulk and rhizosphere soil and between rhizospheres of different plant species are assumed to be strongly governed by quantitative and qualitative rhizodeposit differences. However, data on the relationship between rhizodeposit amounts and BCC are lacking. Other soil microorganisms, e.g. arbuscular mycorrhizal fungi (AMF), may also influence BCC. We simulated foliar herbivory (cutting) to reduce belowground carbon allocation and rhizodeposition of pea plants grown either with or without AMF. This reduced soil respiration, rhizosphere microbial biomass and bacteriovorous protozoan abundance, whereas none of these were affected by AMF. After labelling plants with (13)CO(2), root and rhizosphere soil (13)C enrichment of cut plants were reduced to a higher extent (24-46%) than shoot (13)C enrichment (10-24%). AMF did not affect (13)C enrichment. Despite these clear indications of reduced rhizosphere carbon-input, denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes PCR-amplified targeting DNA and RNA from rhizosphere soil did not reveal any effects of cutting on banding patterns. In contrast, AMF induced consistent differences in both DNA- and RNA-based DGGE profiles. These results show that a reduction in rhizosphere microbial activity is not necessarily accompanied by changes in BCC, whereas AMF presence inhibits proliferation of some bacterial taxa while stimulating others.