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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Pil Joo Kim; Gil Won Kim; Ho Gyeong Chae; Song Rae Cho; +1 Authors

    Plastic film mulching (FM) became a general practice to enhance crop productivity and its net primary production (NPP), but it can increase greenhouse gas (GHG) emissions. The proper addition of organic amendments might effectively decrease the impact of FM on global warming. To evaluate the feasibility of biomass addition on decreasing this negative influence, cover crop biomass as a green manure was incorporated with different recycling levels (0-100% of aboveground biomass) under FM and no-mulching. The net global warming potential (GWP) which integrated with soil C stock change and GHG (N2O and CH4) fluxes with CO2-equivalent was evaluated during maize cultivation. Under the same biomass incorporation, FM significantly enhanced the grain productivity and NPP of maize by 22-61 and 18-58% over no-mulching, respectively. In contrast, FM also highly increased the respired C loss, which was 11-95% higher than NPP increase, over no-mulching. Irrespective with biomass recycling ratio and mulching system, negative NECB which indicates the decrease of soil C stock was observed, mainly due to big harvest removal. FM decreased more soil C stock by 57-158% over no-mulching, but its C stock was clearly increased with increasing biomass addition. FM significantly increased total N2O and CH4 fluxes by 4-61 and 140-600% over no-mulching, respectively. Soil C stock changes mainly decided net GWP scale, but N2O and CH4 fluxes negligibly influenced. As a result, FM highly increased net GWP over no-mulching, while this net GWP was clearly decreased with increasing biomass application. However, cover cropping, and its biomass recycling was not enough to compensate the negative impact of FM on global warming. Therefore, more biomass incorporation might be essential to compensate this negative effect of FM.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao The Science of The T...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    The Science of The Total Environment
    Article . 2022 . Peer-reviewed
    License: Elsevier TDM
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao The Science of The T...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      The Science of The Total Environment
      Article . 2022 . Peer-reviewed
      License: Elsevier TDM
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Kanwalpreet Kour; Deepali Gupta; Kamali Gupta; Sapna Juneja; +3 Authors

    Saffron, also known as “the golden spice”, is one of the most expensive crops in the world. The expensiveness of saffron comes from its rarity, the tedious harvesting process, and its nutritional and medicinal value. Different countries of the world are making great economic growth due to saffron export. In India, it is cultivated mostly in regions of Kashmir owing to its climate and soil composition. The economic value generated by saffron export can be increased manyfold by studying the agronomical factors of saffron and developing a model for artificial cultivation of saffron in any season and anywhere by monitoring and controlling the conditions of its growth. This paper presents a detailed study of all the agronomical variables of saffron that have a direct or indirect impact on its growth. It was found that, out of all the agronomical variables, the important ones having an impact on growth include corm size, temperature, water availability, and minerals. It was also observed that the use of IoT for the sustainable cultivation of saffron in smart cities has been discussed only by very few research papers. An IoT-based framework has also been proposed, which can be used for controlling and monitoring all the important growth parameters of saffron for its cultivation.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Sustainability
    Article . 2022 . Peer-reviewed
    License: CC BY
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Sustainability
    Article . 2022
    Data sources: DOAJ
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Sustainability
      Article . 2022 . Peer-reviewed
      License: CC BY
      Data sources: Crossref
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Sustainability
      Article . 2022
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: null Neha; Gajender Yadav; Rajender Kumar Yadav; Ashwani Kumar; +4 Authors

    Soil salinity and the use of saline groundwater are two major constraints in crop production, which covers a ~1.0 billion ha area of arid and semi-arid regions. The improved drainage function of soil can modify the salty growing environment for higher agricultural production. The present study evaluated the effectiveness of cut-soiler-constructed rice residue-filled preferential shallow subsurface drainage (PSSD) to improve the drainage function and its effect on the yield, quality and plant–water relations of mustard over 2019–2021. Cut-soiler-simulated drains were made in a semi-controlled lysimeter (2 × 2 × 3; L*W*H m) as the main plot treatment in a double replicated split–split experiment with two soil types (subplot) and three irrigation water salinities (4, 8 and 12 dS m−1) as the sub-sub-plot treatment. The drainage volume of variable salinity (EC), dependent on the total water input, was substantially higher in the rainy season (April to October), i.e., 16.6, 7.76 and 12.0% during 2018, 2019 and 2020, with 1.7, 0.32 and 0.77 kg salt removal per lysimeter, compared to the post-rainy season. The mustard seed, straw and biological yields were improved by 31.4, 14.41 and 18.08%, respectively, due to a positive effect on plant–water relations. The mustard seeds produced in the cut-soiler-treated plots recorded higher oil, crude fiber and protein contents and a lower erucic acid content. The increase in salt load, by higher-salinity irrigation water, was also efficiently managed by using cut-soiler PSSD. It was found that the saline irrigation water up to 12.0 dS m−1 can be used under such PSSD without any extra salt loading. The present study showed the potential of cut-soiler PSSD in root zone salinity management by improving drainage in salt-affected arid regions.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Sustainability
    Article . 2022 . Peer-reviewed
    License: CC BY
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Sustainability
    Article . 2022
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Sustainability
      Article . 2022 . Peer-reviewed
      License: CC BY
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Sustainability
      Article . 2022
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Abdul Rehman; Leila Romdhane; Aman Ullah; Muhammad Farooq; +2 Authors

    Two chickpea genotypes viz. Bhakar-2011 (desi) and Noor-2013 (kabuli) were sown in soil filled pots supplied with low (0.3 mg kg-1) and high (3 mg kg-1 soil) zinc (Zn) under control (70% water holding capacity and 25/20 °C day/night temperature), drought (35% water holding capacity) and heat (35/30 °C day/night temperature) stresses. Drought and heat stresses reduced rate of photosynthesis, photosystem II efficiency, plant growth and Zn uptake in chickpea. Low Zn supply exacerbated adverse effects of drought and heat stresses in chickpea, and caused reduction in plant biomass, carbon assimilation, antioxidant activity, impeded Zn uptake and enhanced oxidative damage. However, adequate Zn supply ameliorated adverse effect of drought and heat stresses in both chickpea types. The improvements were more in desi than kabuli type. Adequate Zn nutrition is crucial to augment growth of chickpea plants under high temperature and arid climatic conditions.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Plant Physiology and...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Plant Physiology and Biochemistry
    Article . 2019 . Peer-reviewed
    License: Elsevier TDM
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Plant Physiology and...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Plant Physiology and Biochemistry
      Article . 2019 . Peer-reviewed
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: M. Venkateswar Reddy; M. Venkateswar Reddy; Young-Cheol Chang;

    Medium-chain fatty acids (MCFA) such as caproic, heptanoic, and caprylic acids are monocarboxylic acids, which can be used as precursor molecules to synthesize biodiesel, bioplastics, antimicrobials, and corrosion inhibitors.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Sustainable Energy &...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Sustainable Energy & Fuels
    Article . 2021 . Peer-reviewed
    License: Royal Society of Chemistry Licence to Publish
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Sustainable Energy &...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Sustainable Energy & Fuels
      Article . 2021 . Peer-reviewed
      License: Royal Society of Chemistry Licence to Publish
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Toshihiro Hasegawa; Mayumi Yoshimoto; Kazuhiro Kobayasi; Tsutomu Matsui;

    Flower opening in the early morning helps to avoid sterility of rice (Oryza sativa L.) caused by heat stress at anthesis. Although flower opening time (FOT) is under genetic control, it is also affected by weather, particularly by air temperature (Ta). However, the effects of Ta, solar radiation (Rs), and vapor-pressure deficit (VPD) on rice FOT are unclear, making it difficult to predict FOT. Therefore, we investigated the correlation of FOT with Ta, Rs, and VPD during various periods before anthesis under field conditions. By photographing spikelets at 10-min intervals, we determined the FOT of five cultivars. To evaluate the individual effects of cultivar, Ta, Rs, and VPD on FOT, we constructed general linear models (GLMs) and calculated mean Ta, R s, and VPD every 3 hr from 0000 to 1200. The GLMs revealed that the average Ta, Rs, and VPD between 0600 and 0900 significantly affected FOT (adjusted R 2 =0.399; P <0.001). The standardized partial regression coefficients of Ta and Rs were negative and those of VPD were positive, indicating that higher Ta, higher Rs, and lower VPD in the early morning result in earlier FOT. Moreover, multiple-regression analysis showed that the period affecting FOT the most, and the relative contributions of Ta, Rs, and VPD to FOT differ with the cultivar.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Plant Production Sci...arrow_drop_down
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    Plant Production Science
    Article . 2010 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Plant Production Science
    Article
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Plant Production Science
    Article . 2010
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Plant Production Sci...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Plant Production Science
      Article . 2010 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Plant Production Science
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Plant Production Science
      Article . 2010
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Tomohisa Hasunuma; Shih-Hsin Ho; Ting Ting Chang; Zhuo Liu; +7 Authors

    AbstractBiofuels from microalgae is now a hot issue of great potential. However, achieving high starch productivity with photoautotrophic microalgae is still challenging. A feasible approach to enhance the growth and target product of microalgae is to conduct mixotrophic cultivation. The appropriate acetate addition combined with CO2 supply as dual carbon sources (i.e., mixotrophic cultivation) could enhance the cell growth of some microalgae species, but the effect of acetate‐mediated mixotrophic culture mode on carbohydrate accumulation in microalgae remains unclear. Moreover, there is still lack of the information concerning how to increase the productivity of carbohydrates from microalgae under acetate‐amended mixotrophic cultivation and how to optimize the engineering strategies to achieve the goal. This study was undertaken to develop an optimal acetate‐contained mixotrophic cultivation system coupled with effective operation strategies to markedly improve the carbohydrate productivity of Chlorella sorokiniana NIES‐2168. The optimal carbohydrate productivity of 695 mg/L/d was obtained, which is the highest value ever reported. The monosaccharide in the accumulated carbohydrates is mainly glucose (i.e., 85–90%), which is very suitable for bio‐alcohols fermentation. Hence, by applying the optimal process developed in this study, C. sorokiniana NIES‐2168 has a high potential to serve as a feedstock for subsequent biofuels conversion.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Biotechnology Journa...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Biotechnology Journal
    Article . 2016 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Biotechnology Journa...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Biotechnology Journal
      Article . 2016 . Peer-reviewed
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Chul Ho Kim; Jang Min Park; Seonghun Kim;

    Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Applied Biochemistry...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Applied Biochemistry and Biotechnology
    Article . 2013 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Applied Biochemistry...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Applied Biochemistry and Biotechnology
      Article . 2013 . Peer-reviewed
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Pankaj Kumar; Ebrahem M. Eid; Mostafa A. Taher; Mohamed H. E. El-Morsy; +11 Authors

    Agro-wastes, such as crop residues, leaf litter, and sawdust, are major contributors to global greenhouse gas emissions, and consequently a major concern for climate change. Nowadays, mushroom cultivation has appeared as an emerging agribusiness that helps in the sustainable management of agro-wastes. However, partial utilization of agro-wastes by mushrooms results in the generation of a significant quantity of spent mushroom substrates (SMS) that have continued to become an environmental problem. In particular, Shiitake (Lentinula edodes Berk.) mushrooms can be grown on different types of agro-wastes and also generate a considerable amount of SMS. Therefore, this study investigates the biotransformation of SMS obtained after Shiitake mushroom cultivation into biogas and attendant utilization of slurry digestate (SD) in tomato (Solanum lycopersicum L.) crop fertilization. Biogas production experiments were conducted anaerobically using four treatments of SMS, i.e., 0% (control), 25, 50, and 75% inoculated with a proportional amount of cow dung (CD) as inoculum. The results on biogas production revealed that SMS 50% treatment yielded the highest biogas volume (8834 mL or 11.93 mL/g of organic carbon) and methane contents (61%) along with maximum reduction of physicochemical and proximate parameters of slurry. Furthermore, the biogas digestate from 50% treatment further helped to increase the seed germination (93.25%), seedling length (9.2 cm), seedling root length (4.19 cm), plant height (53.10 cm), chlorophyll content (3.38 mg/g), total yield (1.86 kg/plant), flavonoids (5.06 mg/g), phenolics (2.78 mg/g), and tannin (3.40 mg/g) contents of tomato significantly (p < 0.05) in the 10% loading rate. The findings of this study suggest sustainable upcycling of SMS inspired by a circular economy approach through synergistic production of bioenergy and secondary fruit crops, which could potentially contribute to minimize the carbon footprints of the mushroom production sector.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Horticulturaearrow_drop_down
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    Horticulturae
    Article . 2022 . Peer-reviewed
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    Horticulturae
    Article . 2022
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Horticulturaearrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Horticulturae
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Kim-Cuong Le; Thanh-Tam Ho; Jong-Du Lee; Kee-Yoeup Paek; +1 Authors

    Panax ginseng Mayer is a perennial herb that has been used as a medicinal plant in Eastern Asia for thousands of years. The aim of this study was to enhance root biomass and ginsenoside content in cultured adventitious roots by colchicine mutagenesis. Adventitious P. ginseng roots were treated with colchicine at different concentrations (100, 200, and 300 mg·L−1) and for different durations (1, 2, and 3 days). Genetic variability of mutant lines was assessed using random amplification of polymorphic DNA (RAPD) analysis. Ginsenoside biosynthesis gene expression, ginsenoside content, enzyme activities, and performance in bioreactor culture were assessed in four mutant lines (100–1-2, 100–1-18, 300–1-16, and 300–2-8). The results showed that ginsenoside productivity was enhanced in all mutant lines, with mutant 100–1-18 exhibiting the most pronounced increase (4.8-fold higher than the control). Expression of some ginsenoside biosynthetic enzymes was elevated in mutant lines. Enzyme activities varied among lines, and lipid peroxidation activity correlated with root biomass. All four lines were suitable for bioreactor cultivation, with mutant 100–1-18 exhibiting the highest biomass after culture scale-up. The results indicated that colchicine mutagenesis of P. ginseng roots increased biomass and ginsenosides production. This technique, and the root lines produced in this study, may be used to increase industrial yields of P. ginseng biomass and ginsenosides.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Agronomyarrow_drop_down
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    Agronomy
    Article . 2020 . Peer-reviewed
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Article . 2020
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  • image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Authors: Pil Joo Kim; Gil Won Kim; Ho Gyeong Chae; Song Rae Cho; +1 Authors

    Plastic film mulching (FM) became a general practice to enhance crop productivity and its net primary production (NPP), but it can increase greenhouse gas (GHG) emissions. The proper addition of organic amendments might effectively decrease the impact of FM on global warming. To evaluate the feasibility of biomass addition on decreasing this negative influence, cover crop biomass as a green manure was incorporated with different recycling levels (0-100% of aboveground biomass) under FM and no-mulching. The net global warming potential (GWP) which integrated with soil C stock change and GHG (N2O and CH4) fluxes with CO2-equivalent was evaluated during maize cultivation. Under the same biomass incorporation, FM significantly enhanced the grain productivity and NPP of maize by 22-61 and 18-58% over no-mulching, respectively. In contrast, FM also highly increased the respired C loss, which was 11-95% higher than NPP increase, over no-mulching. Irrespective with biomass recycling ratio and mulching system, negative NECB which indicates the decrease of soil C stock was observed, mainly due to big harvest removal. FM decreased more soil C stock by 57-158% over no-mulching, but its C stock was clearly increased with increasing biomass addition. FM significantly increased total N2O and CH4 fluxes by 4-61 and 140-600% over no-mulching, respectively. Soil C stock changes mainly decided net GWP scale, but N2O and CH4 fluxes negligibly influenced. As a result, FM highly increased net GWP over no-mulching, while this net GWP was clearly decreased with increasing biomass application. However, cover cropping, and its biomass recycling was not enough to compensate the negative impact of FM on global warming. Therefore, more biomass incorporation might be essential to compensate this negative effect of FM.

    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao The Science of The T...arrow_drop_down
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    The Science of The Total Environment
    Article . 2022 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao The Science of The T...arrow_drop_down
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      The Science of The Total Environment
      Article . 2022 . Peer-reviewed
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Kanwalpreet Kour; Deepali Gupta; Kamali Gupta; Sapna Juneja; +3 Authors

    Saffron, also known as “the golden spice”, is one of the most expensive crops in the world. The expensiveness of saffron comes from its rarity, the tedious harvesting process, and its nutritional and medicinal value. Different countries of the world are making great economic growth due to saffron export. In India, it is cultivated mostly in regions of Kashmir owing to its climate and soil composition. The economic value generated by saffron export can be increased manyfold by studying the agronomical factors of saffron and developing a model for artificial cultivation of saffron in any season and anywhere by monitoring and controlling the conditions of its growth. This paper presents a detailed study of all the agronomical variables of saffron that have a direct or indirect impact on its growth. It was found that, out of all the agronomical variables, the important ones having an impact on growth include corm size, temperature, water availability, and minerals. It was also observed that the use of IoT for the sustainable cultivation of saffron in smart cities has been discussed only by very few research papers. An IoT-based framework has also been proposed, which can be used for controlling and monitoring all the important growth parameters of saffron for its cultivation.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Sustainabilityarrow_drop_down
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    Sustainability
    Article . 2022 . Peer-reviewed
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    Article . 2022
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      Sustainability
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    Authors: null Neha; Gajender Yadav; Rajender Kumar Yadav; Ashwani Kumar; +4 Authors

    Soil salinity and the use of saline groundwater are two major constraints in crop production, which covers a ~1.0 billion ha area of arid and semi-arid regions. The improved drainage function of soil can modify the salty growing environment for higher agricultural production. The present study evaluated the effectiveness of cut-soiler-constructed rice residue-filled preferential shallow subsurface drainage (PSSD) to improve the drainage function and its effect on the yield, quality and plant–water relations of mustard over 2019–2021. Cut-soiler-simulated drains were made in a semi-controlled lysimeter (2 × 2 × 3; L*W*H m) as the main plot treatment in a double replicated split–split experiment with two soil types (subplot) and three irrigation water salinities (4, 8 and 12 dS m−1) as the sub-sub-plot treatment. The drainage volume of variable salinity (EC), dependent on the total water input, was substantially higher in the rainy season (April to October), i.e., 16.6, 7.76 and 12.0% during 2018, 2019 and 2020, with 1.7, 0.32 and 0.77 kg salt removal per lysimeter, compared to the post-rainy season. The mustard seed, straw and biological yields were improved by 31.4, 14.41 and 18.08%, respectively, due to a positive effect on plant–water relations. The mustard seeds produced in the cut-soiler-treated plots recorded higher oil, crude fiber and protein contents and a lower erucic acid content. The increase in salt load, by higher-salinity irrigation water, was also efficiently managed by using cut-soiler PSSD. It was found that the saline irrigation water up to 12.0 dS m−1 can be used under such PSSD without any extra salt loading. The present study showed the potential of cut-soiler PSSD in root zone salinity management by improving drainage in salt-affected arid regions.

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    Sustainability
    Article . 2022 . Peer-reviewed
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    Sustainability
    Article . 2022
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      Sustainability
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    Authors: Abdul Rehman; Leila Romdhane; Aman Ullah; Muhammad Farooq; +2 Authors

    Two chickpea genotypes viz. Bhakar-2011 (desi) and Noor-2013 (kabuli) were sown in soil filled pots supplied with low (0.3 mg kg-1) and high (3 mg kg-1 soil) zinc (Zn) under control (70% water holding capacity and 25/20 °C day/night temperature), drought (35% water holding capacity) and heat (35/30 °C day/night temperature) stresses. Drought and heat stresses reduced rate of photosynthesis, photosystem II efficiency, plant growth and Zn uptake in chickpea. Low Zn supply exacerbated adverse effects of drought and heat stresses in chickpea, and caused reduction in plant biomass, carbon assimilation, antioxidant activity, impeded Zn uptake and enhanced oxidative damage. However, adequate Zn supply ameliorated adverse effect of drought and heat stresses in both chickpea types. The improvements were more in desi than kabuli type. Adequate Zn nutrition is crucial to augment growth of chickpea plants under high temperature and arid climatic conditions.

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    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Plant Physiology and Biochemistry
    Article . 2019 . Peer-reviewed
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      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Plant Physiology and Biochemistry
      Article . 2019 . Peer-reviewed
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    Authors: M. Venkateswar Reddy; M. Venkateswar Reddy; Young-Cheol Chang;

    Medium-chain fatty acids (MCFA) such as caproic, heptanoic, and caprylic acids are monocarboxylic acids, which can be used as precursor molecules to synthesize biodiesel, bioplastics, antimicrobials, and corrosion inhibitors.

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    Sustainable Energy & Fuels
    Article . 2021 . Peer-reviewed
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      Sustainable Energy & Fuels
      Article . 2021 . Peer-reviewed
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    Authors: Toshihiro Hasegawa; Mayumi Yoshimoto; Kazuhiro Kobayasi; Tsutomu Matsui;

    Flower opening in the early morning helps to avoid sterility of rice (Oryza sativa L.) caused by heat stress at anthesis. Although flower opening time (FOT) is under genetic control, it is also affected by weather, particularly by air temperature (Ta). However, the effects of Ta, solar radiation (Rs), and vapor-pressure deficit (VPD) on rice FOT are unclear, making it difficult to predict FOT. Therefore, we investigated the correlation of FOT with Ta, Rs, and VPD during various periods before anthesis under field conditions. By photographing spikelets at 10-min intervals, we determined the FOT of five cultivars. To evaluate the individual effects of cultivar, Ta, Rs, and VPD on FOT, we constructed general linear models (GLMs) and calculated mean Ta, R s, and VPD every 3 hr from 0000 to 1200. The GLMs revealed that the average Ta, Rs, and VPD between 0600 and 0900 significantly affected FOT (adjusted R 2 =0.399; P <0.001). The standardized partial regression coefficients of Ta and Rs were negative and those of VPD were positive, indicating that higher Ta, higher Rs, and lower VPD in the early morning result in earlier FOT. Moreover, multiple-regression analysis showed that the period affecting FOT the most, and the relative contributions of Ta, Rs, and VPD to FOT differ with the cultivar.

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    Plant Production Science
    Article . 2010 . Peer-reviewed
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    Plant Production Science
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    Plant Production Science
    Article . 2010
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      Plant Production Science
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      Plant Production Science
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      Plant Production Science
      Article . 2010
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    Authors: Tomohisa Hasunuma; Shih-Hsin Ho; Ting Ting Chang; Zhuo Liu; +7 Authors

    AbstractBiofuels from microalgae is now a hot issue of great potential. However, achieving high starch productivity with photoautotrophic microalgae is still challenging. A feasible approach to enhance the growth and target product of microalgae is to conduct mixotrophic cultivation. The appropriate acetate addition combined with CO2 supply as dual carbon sources (i.e., mixotrophic cultivation) could enhance the cell growth of some microalgae species, but the effect of acetate‐mediated mixotrophic culture mode on carbohydrate accumulation in microalgae remains unclear. Moreover, there is still lack of the information concerning how to increase the productivity of carbohydrates from microalgae under acetate‐amended mixotrophic cultivation and how to optimize the engineering strategies to achieve the goal. This study was undertaken to develop an optimal acetate‐contained mixotrophic cultivation system coupled with effective operation strategies to markedly improve the carbohydrate productivity of Chlorella sorokiniana NIES‐2168. The optimal carbohydrate productivity of 695 mg/L/d was obtained, which is the highest value ever reported. The monosaccharide in the accumulated carbohydrates is mainly glucose (i.e., 85–90%), which is very suitable for bio‐alcohols fermentation. Hence, by applying the optimal process developed in this study, C. sorokiniana NIES‐2168 has a high potential to serve as a feedstock for subsequent biofuels conversion.

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    Biotechnology Journal
    Article . 2016 . Peer-reviewed
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      Biotechnology Journal
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    Authors: Chul Ho Kim; Jang Min Park; Seonghun Kim;

    Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.

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    Applied Biochemistry and Biotechnology
    Article . 2013 . Peer-reviewed
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      Applied Biochemistry and Biotechnology
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    Authors: Pankaj Kumar; Ebrahem M. Eid; Mostafa A. Taher; Mohamed H. E. El-Morsy; +11 Authors

    Agro-wastes, such as crop residues, leaf litter, and sawdust, are major contributors to global greenhouse gas emissions, and consequently a major concern for climate change. Nowadays, mushroom cultivation has appeared as an emerging agribusiness that helps in the sustainable management of agro-wastes. However, partial utilization of agro-wastes by mushrooms results in the generation of a significant quantity of spent mushroom substrates (SMS) that have continued to become an environmental problem. In particular, Shiitake (Lentinula edodes Berk.) mushrooms can be grown on different types of agro-wastes and also generate a considerable amount of SMS. Therefore, this study investigates the biotransformation of SMS obtained after Shiitake mushroom cultivation into biogas and attendant utilization of slurry digestate (SD) in tomato (Solanum lycopersicum L.) crop fertilization. Biogas production experiments were conducted anaerobically using four treatments of SMS, i.e., 0% (control), 25, 50, and 75% inoculated with a proportional amount of cow dung (CD) as inoculum. The results on biogas production revealed that SMS 50% treatment yielded the highest biogas volume (8834 mL or 11.93 mL/g of organic carbon) and methane contents (61%) along with maximum reduction of physicochemical and proximate parameters of slurry. Furthermore, the biogas digestate from 50% treatment further helped to increase the seed germination (93.25%), seedling length (9.2 cm), seedling root length (4.19 cm), plant height (53.10 cm), chlorophyll content (3.38 mg/g), total yield (1.86 kg/plant), flavonoids (5.06 mg/g), phenolics (2.78 mg/g), and tannin (3.40 mg/g) contents of tomato significantly (p < 0.05) in the 10% loading rate. The findings of this study suggest sustainable upcycling of SMS inspired by a circular economy approach through synergistic production of bioenergy and secondary fruit crops, which could potentially contribute to minimize the carbon footprints of the mushroom production sector.

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    Horticulturae
    Article . 2022 . Peer-reviewed
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    Article . 2022
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    Authors: Kim-Cuong Le; Thanh-Tam Ho; Jong-Du Lee; Kee-Yoeup Paek; +1 Authors

    Panax ginseng Mayer is a perennial herb that has been used as a medicinal plant in Eastern Asia for thousands of years. The aim of this study was to enhance root biomass and ginsenoside content in cultured adventitious roots by colchicine mutagenesis. Adventitious P. ginseng roots were treated with colchicine at different concentrations (100, 200, and 300 mg·L−1) and for different durations (1, 2, and 3 days). Genetic variability of mutant lines was assessed using random amplification of polymorphic DNA (RAPD) analysis. Ginsenoside biosynthesis gene expression, ginsenoside content, enzyme activities, and performance in bioreactor culture were assessed in four mutant lines (100–1-2, 100–1-18, 300–1-16, and 300–2-8). The results showed that ginsenoside productivity was enhanced in all mutant lines, with mutant 100–1-18 exhibiting the most pronounced increase (4.8-fold higher than the control). Expression of some ginsenoside biosynthetic enzymes was elevated in mutant lines. Enzyme activities varied among lines, and lipid peroxidation activity correlated with root biomass. All four lines were suitable for bioreactor cultivation, with mutant 100–1-18 exhibiting the highest biomass after culture scale-up. The results indicated that colchicine mutagenesis of P. ginseng roots increased biomass and ginsenosides production. This technique, and the root lines produced in this study, may be used to increase industrial yields of P. ginseng biomass and ginsenosides.

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