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description Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:MDPI AG Aisha Sajjad; Shaheen Begum; Muhammad Adnan; Gul-e-Saba Chaudhry; Muhammad Ibrahim; Mehwish Jamil Noor; Asma Jabeen; Sofia Khalid; Shazia Iram; Yeong Yik Sung; Tengku Sifzizul Tengku Muhammad;doi: 10.3390/su141811219
This research estimates the carbon stock of the subtropical broad-leaved evergreen scrub forest of Lehtrar, a revenue estate of Kotli Sattian, Rawalpindi, Punjab, Pakistan. A total of six nested co-centric plots of 17.84 m2 each were laid out in the forest, having two sub-plots of 5.64 m2 and 1 m2 each, for shrubs and litter, respectively. Stem density, tree height, diameter at breast height (DBH), total tree biomass, and total carbon stock were calculated. In each plot, parameters like latitude, longitude, aspect, slope, elevation, tree count, etc., were catalogued. The carbon value was calculated in pools such as aboveground biomass (AGB), belowground biomass (BGB), litter, shrubs, etc. The tree height was measured using Abney’s level and the diameter at breast height (DBH) with diameter tape, while factors such as volume, shrub mass, litter mass, total tree biomass, and total carbon stock were calculated by using standard formulas. Results showed Olea ferrugineae to be the most abundant tree species in the study area, followed by Acacia modesta. The total average DBH and height were calculated as 17.03 and 16.79, respectively, with the species Dalbergia sissoo having the greatest DBH value. The mean carbon stock came out to be 47.75 tons/ha, with plot number 3 having the highest value of carbon stock, owing to the greatest stem count. The results of the study were significant and reflected a rich stem density, rich biomass, and an adequate carbon stocking capacity. The scrub forests of the study area, being important carbon sinks, are prone to deforestation and forest degradation activities that need to be controlled by using proper forest management practices to keep their carbon sequestration ability intact, as suggested under various reducing emissions from deforestation and forest degradation (REDD initiatives of UNFCCC.
Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 3 citations 3 popularity Average influence Average impulse Average Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:MDPI AG Aisha Sajjad; Shaheen Begum; Muhammad Adnan; Gul-e-Saba Chaudhry; Muhammad Ibrahim; Mehwish Jamil Noor; Asma Jabeen; Sofia Khalid; Shazia Iram; Yeong Yik Sung; Tengku Sifzizul Tengku Muhammad;doi: 10.3390/su141811219
This research estimates the carbon stock of the subtropical broad-leaved evergreen scrub forest of Lehtrar, a revenue estate of Kotli Sattian, Rawalpindi, Punjab, Pakistan. A total of six nested co-centric plots of 17.84 m2 each were laid out in the forest, having two sub-plots of 5.64 m2 and 1 m2 each, for shrubs and litter, respectively. Stem density, tree height, diameter at breast height (DBH), total tree biomass, and total carbon stock were calculated. In each plot, parameters like latitude, longitude, aspect, slope, elevation, tree count, etc., were catalogued. The carbon value was calculated in pools such as aboveground biomass (AGB), belowground biomass (BGB), litter, shrubs, etc. The tree height was measured using Abney’s level and the diameter at breast height (DBH) with diameter tape, while factors such as volume, shrub mass, litter mass, total tree biomass, and total carbon stock were calculated by using standard formulas. Results showed Olea ferrugineae to be the most abundant tree species in the study area, followed by Acacia modesta. The total average DBH and height were calculated as 17.03 and 16.79, respectively, with the species Dalbergia sissoo having the greatest DBH value. The mean carbon stock came out to be 47.75 tons/ha, with plot number 3 having the highest value of carbon stock, owing to the greatest stem count. The results of the study were significant and reflected a rich stem density, rich biomass, and an adequate carbon stocking capacity. The scrub forests of the study area, being important carbon sinks, are prone to deforestation and forest degradation activities that need to be controlled by using proper forest management practices to keep their carbon sequestration ability intact, as suggested under various reducing emissions from deforestation and forest degradation (REDD initiatives of UNFCCC.
Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 3 citations 3 popularity Average influence Average impulse Average Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:Frontiers Media SA Zulaikha Mat Deris; Shumpei Iehata; Han Ming Gan; Mhd Ikhwanuddin; Musa Najiah; Md Asaduzzaman; Min Wang; Min Wang; Yantao Liang; Yantao Liang; Muhd Danish-Daniel; Yeong Yik Sung; Yeong Yik Sung; Li Lian Wong; Li Lian Wong;Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 9 citations 9 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:Frontiers Media SA Zulaikha Mat Deris; Shumpei Iehata; Han Ming Gan; Mhd Ikhwanuddin; Musa Najiah; Md Asaduzzaman; Min Wang; Min Wang; Yantao Liang; Yantao Liang; Muhd Danish-Daniel; Yeong Yik Sung; Yeong Yik Sung; Li Lian Wong; Li Lian Wong;Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 9 citations 9 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu
description Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:MDPI AG Aisha Sajjad; Shaheen Begum; Muhammad Adnan; Gul-e-Saba Chaudhry; Muhammad Ibrahim; Mehwish Jamil Noor; Asma Jabeen; Sofia Khalid; Shazia Iram; Yeong Yik Sung; Tengku Sifzizul Tengku Muhammad;doi: 10.3390/su141811219
This research estimates the carbon stock of the subtropical broad-leaved evergreen scrub forest of Lehtrar, a revenue estate of Kotli Sattian, Rawalpindi, Punjab, Pakistan. A total of six nested co-centric plots of 17.84 m2 each were laid out in the forest, having two sub-plots of 5.64 m2 and 1 m2 each, for shrubs and litter, respectively. Stem density, tree height, diameter at breast height (DBH), total tree biomass, and total carbon stock were calculated. In each plot, parameters like latitude, longitude, aspect, slope, elevation, tree count, etc., were catalogued. The carbon value was calculated in pools such as aboveground biomass (AGB), belowground biomass (BGB), litter, shrubs, etc. The tree height was measured using Abney’s level and the diameter at breast height (DBH) with diameter tape, while factors such as volume, shrub mass, litter mass, total tree biomass, and total carbon stock were calculated by using standard formulas. Results showed Olea ferrugineae to be the most abundant tree species in the study area, followed by Acacia modesta. The total average DBH and height were calculated as 17.03 and 16.79, respectively, with the species Dalbergia sissoo having the greatest DBH value. The mean carbon stock came out to be 47.75 tons/ha, with plot number 3 having the highest value of carbon stock, owing to the greatest stem count. The results of the study were significant and reflected a rich stem density, rich biomass, and an adequate carbon stocking capacity. The scrub forests of the study area, being important carbon sinks, are prone to deforestation and forest degradation activities that need to be controlled by using proper forest management practices to keep their carbon sequestration ability intact, as suggested under various reducing emissions from deforestation and forest degradation (REDD initiatives of UNFCCC.
Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 3 citations 3 popularity Average influence Average impulse Average Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:MDPI AG Aisha Sajjad; Shaheen Begum; Muhammad Adnan; Gul-e-Saba Chaudhry; Muhammad Ibrahim; Mehwish Jamil Noor; Asma Jabeen; Sofia Khalid; Shazia Iram; Yeong Yik Sung; Tengku Sifzizul Tengku Muhammad;doi: 10.3390/su141811219
This research estimates the carbon stock of the subtropical broad-leaved evergreen scrub forest of Lehtrar, a revenue estate of Kotli Sattian, Rawalpindi, Punjab, Pakistan. A total of six nested co-centric plots of 17.84 m2 each were laid out in the forest, having two sub-plots of 5.64 m2 and 1 m2 each, for shrubs and litter, respectively. Stem density, tree height, diameter at breast height (DBH), total tree biomass, and total carbon stock were calculated. In each plot, parameters like latitude, longitude, aspect, slope, elevation, tree count, etc., were catalogued. The carbon value was calculated in pools such as aboveground biomass (AGB), belowground biomass (BGB), litter, shrubs, etc. The tree height was measured using Abney’s level and the diameter at breast height (DBH) with diameter tape, while factors such as volume, shrub mass, litter mass, total tree biomass, and total carbon stock were calculated by using standard formulas. Results showed Olea ferrugineae to be the most abundant tree species in the study area, followed by Acacia modesta. The total average DBH and height were calculated as 17.03 and 16.79, respectively, with the species Dalbergia sissoo having the greatest DBH value. The mean carbon stock came out to be 47.75 tons/ha, with plot number 3 having the highest value of carbon stock, owing to the greatest stem count. The results of the study were significant and reflected a rich stem density, rich biomass, and an adequate carbon stocking capacity. The scrub forests of the study area, being important carbon sinks, are prone to deforestation and forest degradation activities that need to be controlled by using proper forest management practices to keep their carbon sequestration ability intact, as suggested under various reducing emissions from deforestation and forest degradation (REDD initiatives of UNFCCC.
Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 3 citations 3 popularity Average influence Average impulse Average Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2022License: CC BYData sources: Multidisciplinary Digital Publishing Instituteadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su141811219&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:Frontiers Media SA Zulaikha Mat Deris; Shumpei Iehata; Han Ming Gan; Mhd Ikhwanuddin; Musa Najiah; Md Asaduzzaman; Min Wang; Min Wang; Yantao Liang; Yantao Liang; Muhd Danish-Daniel; Yeong Yik Sung; Yeong Yik Sung; Li Lian Wong; Li Lian Wong;Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 9 citations 9 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2022Publisher:Frontiers Media SA Zulaikha Mat Deris; Shumpei Iehata; Han Ming Gan; Mhd Ikhwanuddin; Musa Najiah; Md Asaduzzaman; Min Wang; Min Wang; Yantao Liang; Yantao Liang; Muhd Danish-Daniel; Yeong Yik Sung; Yeong Yik Sung; Li Lian Wong; Li Lian Wong;Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 9 citations 9 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3389/fmars.2022.974217&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu