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Land use and climate change always induce significant changes in various parameters of the hydrologic cycle (e.g., surface runoff, infiltration, evapotranspiration). The Wadi El-Assiuti downstream area in the Eastern Desert of Egypt is one of the most promising areas for development that is suffering from insufficient water availability and inadequate water quality for different purposes. The main goal of this research is to evaluate the changes in groundwater quality, land use, and climate in association with geology and flooding during three periods within the years 1997–2019 in the downstream portion of Wadi El-Assiuti in the Eastern Desert of Egypt, using spatiotemporal variation associated with groundwater hydrochemical analysis and GIS techniques. About 133 groundwater samples were collected to examine groundwater quality changes over time. Different groundwater quality indices were calculated, and the results show that TDS levels of groundwater in the study area ranged between 1080–2780 mg/L, 672–4564 mg/L, and 811–6084 mg/L, while SAR levels varied within 6.15–15.34, 1.83–28.87, and 1.43–30.57 for the years 1997, 2007, and 2019, respectively. Both RSBC and SSP values exhibited significantly increasing trends over time. KR values were within 1.36–4.06 in 1997, 0.58–14.09 in 2007, and 0.35–14.92 in 2019; MAR values were within 6.9–45.2 in 1997, 20.79–71.5 in 2007, and 17.71–75.81 in 2019; and PI values were within 60.16–83 in 1997, 45.56–101.03 in 2007, and 42.51–148.88 in 2019. Across the entire study area, ongoing land use changes increased from 1.1% in 1997 to 4.1% in 2019. Findings pointed to the significant contribution of the deep Nubian Sandstone Aquifer to the groundwater aquifer at Wadi El-Assiuti through fractures and deep faults. Given the climatic conditions from 1997–2019, these changes may have affected water quality in shallow aquifers, especially with increasing evaporation. Realizing the spatiotemporal variation of the aquifer recharge system, land use development, and climate change clearly would help in water resource management. This study revealed that flooding events, deep-seated geologic structures, and land use development associated with human activities have the highest impact on groundwater quality.

The food and agricultural industries have numerous practical advantages to be gained from the use of cold plasma technology. This paper attempts to showcase the possible uses of cold plasma in the food sector, while also highlighting the most recent developments and market trends. The efficiency of cold plasma in enhancing food products’ quality and shelf life has been demonstrated in several investigations. This review has concentrated on current research into how this technology affects various food chain production stages. Cold plasma has become a cutting-edge non-thermal technique that can be used to ensure food safety. The precise mechanism underlying the effectiveness of cold plasma is still unclear. Understanding these mechanisms and potential elements that can restrict or increase their effectiveness and results is crucial to further enhancing and implementing cold plasma treatment in food processing. The main objective of this review is to investigate the use of plasma, its exceptional characteristics, and its advantages in safe, sustainable food production. In particular, this review summarizes recent studies on the use of cold plasma for microorganisms and pesticides treatment, compiling them and discussing their content. As reported in the literature, a critical point has also been reviewed about some diverse plasma configurations. A comparative study of the efficacy of cold plasma in environmental applications (microorganisms/pesticides) has also been reviewed from the literature.

Institutional linkages and information flow between agricultural organizations play a critical role in addressing sustainability issues and promoting agrarian innovation. The aim of this study was to evaluate institutional relations and information between the various actors within the agricultural knowledge and information system (AKIS). The study focused on eight actors within the AKIS in Dakahlia governorate of Egypt, namely policy, extension, research, agricultural cooperatives, higher education, secondary education, credit, and the private sector. Thus, the survey sample included 11 representatives of each actor with 88 respondents. Data were collected by a standardized questionnaire distributed online. The graph theoretical technique was used for the quantitative assessment of information flow and institutional linkages established among actors. The findings indicated that agricultural extension ranked first about their real cause and effect on the rest of the system, having a value of 7.95. Two critical information pathways within the AKIS sustained innovation outcomes: (1) higher education–extension–agricultural cooperatives, (2) research–extension–agricultural cooperatives. The results also revealed that agricultural cooperatives ranked second after the extension component on the extent of supplying information to other members in the AKIS, with a value of 4.8. In contrast, the highest component received information from other components (7.6). By analyzing institutional linkages and information flow, this article gives insights to policymakers on the mechanisms that still need to be strengthened and the information gaps between actors to address the challenges of sustainable rural development.

Although plant-based culture media enhances in vitro cultivation of rhizobacteria, studies assessing their biomass potential for large-scale applications are lacking. Here, we advance plant pellets (PPs) as a novel technology to unlock the potential of such vegan culture media for biomass production of Rhizobium leguminosarum. PP formulations were based on mixtures of Egyptian clover powder and the agro-byproducts glycerol and molasses. These mixtures were either contained or not contained in teabags during culture media preparation. Metrics of biomass included colony forming units, optical density (OD600nm), and cell dry weight (DW). Biomass comparisons between culture media based on PPs and standard yeast extract mannitol (YEM) revealed that the following PPs composition, contained in teabags, cultivated rhizobia at levels comparable to YEM: 16 g clover powder, 5% molasses, and 0.8% glycerol. This PPs composition enabled shorter generation times of rhizobia (PP: 3.83 h, YEM: 4.28 h). Strikingly, PPs mixtures supplemented with 10% molasses and not contained in teabags promoted rhizobia without apparent lag phases and produced 25% greater DW than YEM. PPs potentiate the use of dehydrated vegan feedstocks for both plant microbiota cultivation and biomass production and appear as cost- and labor-effective tools, easy to handle and store for plant-based culture media preparation.

Endophytes and rhizospheric microorganisms support invasive species’ adaptation to environmental stresses. Here, we review the impacts of endophytes, rhizospheric microbes (particularly symbiotic nitrogen-fixers), mycorrhiza and pathogens on plant invasion in arid and semi-arid areas. Endophytes and soil microorganisms either enhance nutrient acquisition for enhancing the invasive plant immune system and/or negatively affect native plants. In addition, the positive feedback between mycorrhizal fungi and invasive plants enhances the competitive ability of the aliens, providing them more opportunities for success, establishment, and dominance. The microbes and their secondary metabolites promote invasive plant species by changing soil microbial community structure and carbon biomass as well as enzyme activity, which improves soil properties and processes. The negative impact of invasive exotic plants on the associated biota and the role of allelochemicals are also discussed. It could be concluded that endophytes interact with rhizosphere microbes to promote invasive plant species in arid and semi-arid areas in a way similar to what happens in other ecosystems; the differences are in the pathways and reactions, which depend upon the prevailing abiotic factors. More interdisciplinary field experiments integrating microbial, biotechnological, and molecular approaches are needed to understand the role of symbiotic microbes in invasion biology.

This study tested the anti-hyperlipidemic, hypoglycemic, hepatoprotective, and anti-inflammatory effects of whole pearl millet grain powder (MPG) and its ethanol extract (MPGethaolE) in obese rats fed a high-fat diet. The rats were divided into eight groups based on the treatments they received: control, high fat diet (HFD), HFD + MGE (25 mg/Kg), HFD + MPGethaolE (50 mg/Kg), HFD + MPGethaolE (100 mg/Kg), HFD + MPG (10%), HFD + MPG (20%), and HFD + MPG (30%). The final body weight, visceral, epididymal fat pads, and the liver weight were significantly decreased, in a dose-dependent manner, in HFD fed rats that were co-administered either the MPG powder or MPGethaolE. In the same line, serum levels of triglycerides (TGs), cholesterol (CHOL), and low-density lipoprotein-cholesterol (LDL-c), as well as fasting glucose, insulin, HOMA-IR, and serum levels of lipopolysaccharides (LPS), interleukine-6 (IL-6), interleukine-10 (IL-10), C-reactive protein (CRP), tumor necrosis factor (TNF-α), and adiponectin were progressively decreased while serum levels of high-density lipoproteins (HDL-c) were significantly increased when increasing the doses of both treatments. In conclusion, both the raw powder and ethanolic extract of MP have a comparative dose-dependent anti-obesity, hypoglycemic, hypolipidemic, anti-inflammatory, and anti-steatotic in HFD-fed rats.

Abstract Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-Carotene is generated from lycopene by lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco (Nicotiana tabacum cv. Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA1 content was reduced. RNA interference (RNAi) NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and light-saturated capacity of linear electron transport) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue these phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial or detrimental for plant growth.