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We have characterized the general properties of the heat shock response of the Gram-positive hardy bacterium Enterococcus faecalis. The heat resistance (60 degrees C or 62.5 degrees C, 30 min) of log phase cells of E. faecalis grown at 37 degrees C was enhanced by exposing cells to a prior heat shock at 45 degrees C or 50 degrees C for 30 min. These conditioning temperatures also induced ethanol (22%, v/v) tolerance. The onset of thermotolerance was accompanied by the synthesis of a number of heat shock proteins. The most prominent bands had molecular weights in the range of 48 to 94kDa. By Western blot analysis two of them were found to be immunologically related to the well known DnaK (72kDa) and GroEL (63kDa) heat shock proteins of Escherichia coli. Four other proteins showing little or no variations after exposure to heat are related to DnaJ, GrpE and Lon (La) E. coli proteins and to the Bacillus subtilis sigma 43 factor. Ethanol (2% or 4%, v/v) treatments elicited a similar response although there was a weaker induction of heat shock proteins than with heat shock.

Anaerobic co-digestion of food waste (FW) and pig manure (PM) was undertaken in batch mode at 37°C in order to identify and quantify the synergistic effects of co-digestion on the specific methane yield (SMY) and reaction kinetics. The effects of the high initial volatile fatty acid (VFA) concentrations in PM on synergy observed during co-digestion, and on kinetic modelling were investigated. PM to FW mixing ratios of 1/0, 4/1, 3/2, 2/3, 1/4 and 0/1 (VS basis) were examined. No VFA or ammonia inhibition was observed. The highest SMY of 521±29ml CH4/gVS was achieved at a PM/FW mixing ratio of 1/4. Synergy in terms of both reaction kinetics and SMY occurred at PM/FW mixing ratios of 3/2, 2/3 and 1/4. Initial VFA concentrations did not explain the synergy observed. Throughout the study the conversion of butyric acid was inhibited. Due to the high initial VFA content of PM, conventional first order and Gompertz models were inappropriate for determining reaction kinetics. A dual pooled first order model was found to provide the best fit for the data generated in this study. The optimal mixing ratio in terms of both reaction kinetics and SMY was found at a PM/FW mixing ratio of 1/4.

With the spotlight on smart grid development around the world, it is critical to recognize the key factors contributing to changing power system characteristics. This is more apparent in distribution systems with the integration of renewable energy sources, energy storage, and microgrid development. Utilities are also focusing on the reliability and resiliency of the grid. These activities require distribution automation (DA) strategies that take advantage of available technologies while promoting newer solutions. It is necessary to create a roadmap for holistic DA strategies in a smarter grid. Sustainable and resilient grid development is a paradigm shift requiring a new line of thinking in the engineering, operation, and maintenance of the power system. International perspectives on DA are also addressed, with the understanding that one solution will not fit all. Integrating technical, business, and policy decisions into the challenges will generate the development of technologies, standards, and implementation of the overall solution. The challenges in the development of industry standards are also discussed. This paper explores the challenges and opportunities in the changing landscape of the distribution systems. Evolution of technologies and the business case for infrastructure investment in distribution systems are covered in another paper by the same authors.

Abstract Mediterranean Hydrogen Solar (MedHySol) is a federator project for development of a massive hydrogen production starting from solar energy and its exportation within a framework of a Euro–Maghrebian Cooperation project for industrial and energetic needs in the Mediterranean basin. The proposal of this project is included in the Algiers Declaration's on Hydrogen from Renewable Origin following the organization of the first international workshop on hydrogen which was held in 2005. Algeria is the privileged site to receive the MedHySol platform. The objective of the first step of the project is to realize a technological platform allowing the evaluation of emergent technologies of hydrogen production from solar energy with a significant size (10–100 kW) and to maintain the development of energetic rupture technologies. The second step of the project is to implement the most effective and less expensive technologies to pilot great projects (1–1000 MW). In this article we present the potentialities and the feasibility of MedHySol, as well as the fundamental elements for a scientific and technical supervision of this great project.

Abstract Novel proton-conducting electrolytes were prepared from the sol–gel precursor 1-[3-(trimethoxy-λ4-silyl)propyl]imidazole with the addition of either trifluoroacetic or acetic acid. The presence of trimethoxysilyl groups enabled the solvolysis and condensation reactions of silsesquioxane species. IR spectroscopy revealed that more cube-like species formed in the electrolyte prepared from trifluoroacetic acid, while cube- and ladder-like silsesquioxanes were present in the electrolyte with acetic acid. This assignation was independently confirmed by 29Si NMR analyses revealing the T3 signals of trisiloxane bonding. IR spectroscopy also pointed to the formation of hydrogen bonding in the latter electrolyte, since the frequencies of the observed bands at 1710, 1409, and 1272 cm−1 approached those of acetic acid. In contrast, the IR bands at 1662, 1204, and 1130 cm−1 confirmed the existence of trifluoroacetate anions in the case when the electrolyte was prepared from trifluoroacetic acid. The presence of free trifluoroacetate anions contributed to the moderately higher specific conductivity of this electrolyte (4.6×10−5 S/cm) compared to that of acetic acid (1.6×10−5 S/cm). The specific conductivity of the electrolytes could be further increased by the addition of a lithium salt. All electrolytes were employed in electrochromic devices with optically active WO3 and various inorganic counter-electrodes (CeVO4, V2O5, Ti/V-oxide). Photopic transmittance changes from 30% to 40% were achieved.

Abstract The importance of integrating resources criticality assessment into Life Cycle Assessment (LCA) under the Life Cycle Sustainability Assessment (LCSA) framework has been discussed for some time. However, the question how to proceed towards integration remain unclear. Only very few work is published on this issue and to our knowledge only Schneider et al. (2014) have developed one operational model. In this paper we review existing literature critically and explain why integration is important and how it could be done, using knowledge from outside the LCA field. The criticality assessment method proposed by Graedel et al. (2012) is identified as the best starting point. This paper shows based on a critical review why bridging towards such a method could help addressing criticality in LCSA, what are possible options and which research needs exists. It reveals that currently LCA does not adequately cover resource criticality and that methods like Graedel et al. (2012) are inspiring further development of LCA; both approaches have a complementary nature and hence could be developed towards integration within the LCSA framework. Currently resource indicators are not meaningful in LCA, wherefore there is no consensus in LCA community on what to recommend; hence the Area of Protection Natural Resources needs to be rethought. Broadening the scope of LCA towards LCSA provides a conceptual framework to keep LCA relevant for assessing sustainability challenges like access to resources.

The study empirically examines the environmental Kuznets curve (EKC) hypotheses by investigating the relationship between ecological footprint, economic growth, energy consumption, and population growth. The study uses ecological footprint as a measurement of environmental degradation which is a more comprehensive indicator and considers all factors responsible for environmental degradation. Keeping in view the problem of cross-sectional dependence, a more efficient estimation tools like pooled mean group and augmented mean group have been used to estimate the long-run parameters for 22 European countries from 1995 through 2015. Results of the study found a quadratic relationship between income growth and ecological footprint and support validity of EKC. Energy consumption positively contributes to ecological footprint, while population growth plays no significant role in determining environmental quality. The long-run estimates of the study are validated through robustness analysis by employing dynamic ordinary least square (DOLS) and fully modified ordinary least square (FMOLS) techniques. Dumitrescu and Hurlin (2012) panel non-causality test indicated that there is a unidirectional causality running from GDP to ecological footprint while bidirectional causality running between energy consumption and ecological footprint. The study identified that population growth in European region is not a severe issue as compared to intensive energy consumption. Policies which restrict emission, deforestation, and water pollution should be adopted for sustainability of environment.

AbstractRenewable energy sources and electric automotive transportation are popular topics in our belated energy‐conscious society, placing electrochemical energy management as one of the major technological developments for this new century. Besides efficiency, any new storage technologies will have to provide advantages in terms of cost and environmental footprint and thus rely on sustainable materials that can be processed at low temperature. To meet such challenges future devices will require inspiration from living organisms and rely on either bio‐inspired or biomimetic approaches.

Chronic alcohol consumption in adults can induce cardiomyopathy, arrhythmias, and heart failure. In newborns, prenatal alcohol exposure can increase the risk of congenital heart diseases. Understanding biological mechanisms involved in the long-term alcohol exposure-induced cardiotoxicity is pivotal to the discovery of therapeutic strategies. In this study, cardiomyocytes derived from human pluripotent stem cells (hiPSC-CMs) were treated with clinically relevant doses of ethanol for various durations up to 5 weeks. The treated cells were characterized for their cellular properties and functions, and global proteomic profiling was conducted to understand the molecular changes associated with long-term ethanol exposure. Increased cell death, oxidative stress, deranged Ca2+ handling, abnormal action potential, altered contractility, and suppressed structure development were observed in ethanol-treated cells. Many dysregulated proteins identified by global proteomic profiling were involved in apoptosis, heart contraction, and extracellular collagen matrix. In addition, several signaling pathways including the Wnt and TGFβ signaling pathways were affected due to long-term ethanol treatment. Therefore, chronic ethanol treatment of hiPSC-CMs induces cardiotoxicity, impairs cardiac functions, and alters protein expression and signaling pathways. This study demonstrates the utility of hiPSC-CMs as a novel model for chronic alcohol exposure study and provides the molecular mechanisms associated with long-term alcohol exposure in human cardiomyocytes.

AbstractThe unhairing step in leather manufacture generates a highly hazardous and alkaline wastewater. This article reports the evaluation of an activated sludge system for the treatment of unhairing wastewater and effluent detoxification, assessed by seed germination tests.The activated sludge system reactor was fed for 112 days with diluted unhairing effluent; the operation strategy included increasing the organic loading rate (OLR) from 0.7 to 1.6 g chemical oxygen demand (COD) L−1 d−1. COD and suspended solids (SS) removal efficiencies were up to 85 and 80%, respectively, for an OLR lower than 1.4 g COD L−1 d−1.Sulphide removal efficiency was ∼90%, as sulphide was oxidized to other species such as sulphate. The biological oxidation of thiosulphates into tetrathionates was also investigated.The effect of untreated and treated unhairing wastewater on seed germination of maize, sorghum, and wheat was examined. Treatment decreased the phytotoxicity of the wastewater. Indeed, germination was inhibited when effluent dilution was lower than 90% of untreated wastewater, whereas a positive effect of treated wastewater was noticed.Phytotoxicity assays showed that biological treatment of unhairing wastewater contributed to a decrease in toxicity of the effluent. © 2010 American Institute of Chemical Engineers Environ Prog, 2011