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Apigenin (AP) has many pharmacological activities. AP has poor solubility in some solvents. AP is insoluble in water and slightly soluble in ethanol (1.93 mg/ml). It has limited application and exploitation. Therefore, the liquid antisolvent precipitation (LAP) method was applied to improve the solubility of AP in ethanol by changing its crystal form or producing ultra-fine particles. Then, the inclusion complex of AP with 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) is prepared using the solvent removal method. The effects of various experimental parameters on the solubility of AP in ethanol were investigated through the single factor design. Under the optimum conditions, the AP-ethanol solution of 6.19 mg/ml was obtained. The inclusion complex of AP with HP-β-CD was obtained by the solvent removal method. The load efficiency (LE) and drug encapsulation efficiency (EE) of the inclusion complex of AP with HP-β-CD were 13.98%±0.14% and 97.86%±1.07%, respectively. SEM, FTIR, 1HNMR, XRD, DSC and TG were used to analyze the characteristics of the inclusion complex of AP with HP-β-CD. These results showed that the inclusion complex has significantly different characteristics with AP. In addition, the dissolution rate and solubility of the inclusion complex were approximately 15.24 and 68.7 times higher than AP in artificial gastric juice, and was separately 10.4 times and 40.05 times higher than AP in artificial intestinal juice. The bioavailability of inclusion complex increased 3.97 times compared with AP.

We evaluate the potential of using a process-based ecosystem model (BEPS) for crop biomass mapping at 20 m resolution over the research site in Manitoba, western Canada driven by spatially explicit leaf area index (LAI) retrieved from Sentinel-2 spectral reflectance throughout the entire growing season. We find that overall, the BEPS-simulated crop gross primary production (GPP), net primary production (NPP), and LAI time-series can explain 82%, 83%, and 85%, respectively, of the variation in the above-ground biomass (AGB) for six selected annual crops, while an application of individual crop LAI explains only 50% of the variation in AGB. The linear relationships between the AGB and these three indicators (GPP, NPP and LAI time-series) are rather high for the six crops, while the slopes of the regression models vary for individual crop type, indicating the need for calibration of key photosynthetic parameters and carbon allocation coefficients. This study demonstrates that accumulated GPP and NPP derived from an ecosystem model, driven by Sentinel-2 LAI data and abiotic data, can be effectively used for crop AGB mapping; the temporal information from LAI is also effective in AGB mapping for some crop types.

AbstractThis study examined the influence of pectinase‐producing non‐Saccharomyces yeasts on the chemical and sensory attributes of red and white wines with added pectin. Merlot and Chardonnay wines were produced with or without a mixture of pectinase‐producing non‐Saccharomyces yeasts (Cryptococcus adeliensis, Issatchenkia orientalis, and Pichia kluyveri) added to the must prior to alcoholic fermentation conducted by a commercial strain of Saccharomyces cerevisiae. To ensure sufficient substrate was present, varying concentrations of apple pectin (up to 1.25 g/L for red wines and 1.00 g/L for white wine) were added at the start of fermentation. After bottling, trained panelists (n = 10) analyzed these wines for aroma, flavor, taste, and mouthfeel attributes. For both wines, significant interactions were noted between the presence of non‐Saccharomyces yeasts and pectin addition which affected pH, titratable acidity, and concentrations of D‐galacturonic acid. While no significant sensory differences were observed among the red wines, limited changes were noted for white wines. However, a strong positive correlation was found between the D‐galacturonic acid and buttery aroma for Chardonnay and with flavor for Merlot. Increasing D‐galacturonic acid concentrations, through utilization of non‐Saccharomyces yeasts, may improve the wine quality as a buttery aroma is often associated with high‐quality Chardonnay. For both red and white wines, the utilization of these particular non‐Saccharomyces yeasts significantly influenced chemical properties but yielded minor sensory changes without any faults.Practical ApplicationWith the recent trend to reduce alcohol content in commercial wines, the interest in non‐Saccharomyces yeasts has grown. This study showed that the addition of non‐Saccharomyces yeasts, perhaps due to their pectinase activity, influenced the chemical characteristics of red and white wines with limited sensory differences, making these yeasts a useful tool for winemakers to modify wine properties.

Abstract In this research, we evaluated the intent of engaged private forest landowners to supply woody biomass for bioenergy production. The study was conducted in a U.S. state (Kentucky) where private individuals own a majority (78%) of the state's forest resources. Intent of family forest owners was measured using a mail-based survey. We used the Theory of Planned Behavior to model factors that affect landowner intention, and we tested the effect of educational materials on participates' reported intent. Two-thirds of respondents indicated that they intend to include energy wood in future harvests, but the educational material treatment did not affect intentions. Respondents' attitudes, perceived subjective norms, and perceived control each had a significant effect on intent to harvest. No demographic or land ownership characteristics had an effect on behavioral intent. The only prior harvest activity that significantly increased intent was whether the subject had harvested pulpwood from their forest in the past. Respondents identified barriers that may prevent them from harvesting energy wood, providing forestry professionals with a list of challenges to overcome if supply is to be maximized. Lack of bioenergy markets and woodland access issues were the most frequently reported barriers.

Abstract Eucalyptus plantations in the Southern United States offer a viable feedstock for renewable bioenergy. Delivered cost of eucalypt biomass to a bioenergy facility was simulated in order to understand how key variables affect biomass delivered cost. Three production rates (16.8, 22.4 and 28.0 Mg ha −1 y −1 , dry weight basis) in two investment scenarios were compared in terms of financial analysis, to evaluate the effect of productivity and land investment on the financial indicators of the project. Delivered cost of biomass was simulated to range from $55.1 to $66.1 per delivered Mg (with freight distance of 48.3 km from plantation to biorefinery) depending on site productivity (without considering land investment) at 6% IRR. When land investment was included in the analysis, delivered biomass cost increased to range from $65.0 to $79.4 per delivered Mg depending on site productivity at 6% IRR. Conversion into cellulosic ethanol might be promising with biomass delivered cost lower than $66 Mg −1 . These delivered costs and investment analysis show that Eucalyptus plantations are a potential biomass source for bioenergy production for Southern U.S.

Fungal and bacterial carbon dioxide (CO2) production/emission was determined under a range of redox conditions in sediment from a Louisiana swamp forest used for wastewater treatment. Sediment was incubated in microcosms at 6 Eh levels (-200, -100, 0, +100, +250 and +400 mV) covering the anaerobic range found in wetland soil and sediment. Carbon dioxide production was determined by the substrate-induced respiration (SIR) inhibition method. Cycloheximide (C15H23NO4) was used as the fungal inhibitor and streptomycin (C21H39N7O12) as the bacterial inhibitor. Under moderately reducing conditions (Eh > +250 mV), fungi contributed more than bacteria to the CO2 production. Under highly reducing conditions (Eh or = +100 mV), the F/B ratios for microbial biomass C and N were higher than that for highly reducing conditions (Eh or = +100 mV), the C/N microbial biomass ratio for fungi (C/N: 13.54-14.26) was slightly higher than for bacteria (C/N: 9.61-12.07). Under highly reducing redox conditions (Eh < or = 0 mV), the C/N microbial biomass ratio for fungi (C/N: 10.79-12.41) was higher than for bacteria (C/N: 8.21-9.14). For bacteria and fungi, the C/N microbial biomass ratios under moderately reducing conditions were higher than that in highly reducing conditions. Fungal CO2 production from swamp forest could be of greater ecological significance under moderately reducing sediment conditions contributing to the greenhouse effect (GHE) and the global warming potential (GWP). However, increases in coastal submergence associated with global sea level rise and resultant decrease in sediment redox potential from increased flooding would likely shift CO2 production to bacteria rather than fungi.

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, cassava chips and pellets, were analysed for nutrient composition prior to feed formulation. The cassava chips and pellets contained 2.2% and 2.1% crude protein; 1.2% and 1.5% crude fat; and 75.1% and 67.8% starch, respectively. Lysine and methionine were 0.077%, 0.075%, and 0.017%, 0.020% protein material, respectively, while calculated ME was 12.6 and 11.7 MJ/kg, respectively. Feed intake to day 21 was lower (p<0.01) on the diet containing cassava chips compared to diets with cassava pellets. Enzyme supplementation increased (p<0.01) feed intake on all diets. Live weight at day 21 was significantly (p<0.01) reduced on the diet based on cassava chips compared to pellets, but an improvement (p<0.01) was noticed with the enzyme supplementation. Metabolizable energy intake was reduced (p<0.01) by both cassava chips and pellets, but was increased (p<0.01) on all diets by enzyme supplementation. The NEp was higher (p<0.01) in the maize-based diets than the diets containing cassava. Enzyme supplementation improved (p<0.01) NEp in all the diets. Heat production was highest (p<0.01) on diets containing cassava pellets than on cassava chips. It is possible to use cassava pellets in diets for broiler chickens at a level close to 50% of the diet to reduce cost of production, and the nutritive value of such diets can be improved through supplementation of enzyme products containing carbohydrases, protease, and phytase.

ABSTRACTConcern over increasing water scarcity has led to the introduction of the concept of agricultural water productivity and an emphasis on interventions to achieve ‘more crop per drop’. Yet, a...