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This paper addresses the current knowledge on climate change impacts on mass movement activity in mountain environments by illustrating characteristic cases of debris flows, rock slope failures and landslides from the French, Italian, and Swiss Alps. It is expected that events are likely to occur less frequently during summer, whereas the anticipated increase of rainfall in spring and fall could likely alter debris-flow activity during the shoulder seasons (March, April, November, and December). The magnitude of debris flows could become larger due to larger amounts of sediment delivered to the channels and as a result of the predicted increase in heavy precipitation events. At the same time, however, debris-flow volumes in high-mountain areas will depend chiefly on the stability and/or movement rates of permafrost bodies, and destabilized rock glaciers could lead to debris flows without historic precedents in the future. The frequency of rock slope failures is likely to increase, as excessively warm air temperatures, glacier shrinkage, as well as permafrost warming and thawing will affect and reduce rock slope stability in the direction that adversely affects rock slope stability. Changes in landslide activity in the French and Western Italian Alps will likely depend on differences in elevation. Above 1500 m asl, the projected decrease in snow season duration in future winters and springs will likely affect the frequency, number and seasonality of landslide reactivations. In Piemonte, for instance, 21st century landslides have been demonstrated to occur more frequently in early spring and to be triggered by moderate rainfalls, but also to occur in smaller numbers. On the contrary, and in line with recent observations, events in autumn, characterized by a large spatial density of landslide occurrences might become more scarce in the Piemonte region.

Forest trees dominate many Alpine landscapes that are currently exposed to changing climate. Norway spruce is one of the most important conifer species of the Italian Alps, and natural populations are found across steep environmental gradients with large differences in temperature and moisture availability. This study seeks to determine and quantify patterns of genetic diversity in natural populations toward understanding adaptive responses to changing climate. Across the Italian species range, 24 natural stands were sampled with a major focus on the Eastern Italian Alps. Sampled trees were genotyped for 384 selected single nucleotide polymorphisms (SNPs) from 285 genes. A wide array of potential candidate genes was tested for correlation with climatic parameters. To minimize false-positive association between genotype and climate, population structure was investigated. Pairwise F ST estimates between sampled populations ranged between 0.000 and 0.075, with the highest values involving the two disjoint populations, Valdieri, on the western Italian Alps, and Campolino, the most southern population on the Apennines. Despite considerable genetic admixture among populations, both Bayesian and multivariate approach identified four genetic clusters. Selection scans revealed five F ST outliers, and the environmental association analysis detected ten SNPs associated to one or more climatic variables. Overall, 13 potentially adaptive loci were identified, three of which have been reported in a previous study on the same species conducted on a broader geographical scale. In our study, precipitation, more than temperature, was often associated with genotype; therefore, it appears as the most important environmental variable associated with the high sensitivity of Norway spruce to soil water supply. These findings provide relevant information for understanding and quantifying climate change effects on this species and its ability to genetically adapt.

AbstractThe synthesis and determination of the structure of a Förster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of oligonucleotides conjugated to quantum dots was determined by capillary electrophoresis separation. The results indicate that one to four molecules of oligonucleotide are conjugated to the surface of a single nanoparticle. This conclusion is confirmed by the course of the dependence of Förster resonance energy transfer efficiency on the concentration of fluorescently labeled complementary single‐stranded nucleic acid, showing saturation. While the energy transfer efficiency of the probe hybridized with complementary nucleic acid strands was 30%, negligible efficiency was observed with a noncomplementary strand.

Abstract Vernacular architecture is still very popular and constructed widely in North-East India. In this paper, the result of long-term monitoring of two vernacular houses selected one in Tezpur (warm and humid climate) and other one in Cherrapunjee (cold and cloudy climate) are presented. Long-term monitoring work includes the measurements of temperature (inside and outside house), relative humidity (inside and outside house) and illumination level (inside and outside house) for 25 days in all the seasons (January: winter, April: spring/pre-summer, July: summer/monsoon and October: autumn/pre-winter) of the year 2008. Temperatures profile across all the seasons represents strong daily and seasonal fluctuations. Formulae have been developed based on part of the monitoring data to predict the indoor maximum, average and minimum temperatures inside the same house occupied by the same family. The predicted formulae were developed based on the measured data for the month of January and July and were validated with the measured data of April and October months. It is found that the correlation coefficient ( R 2 value) is above 0.96 for all the six formulae for the entire monitoring period.

Saccharomyces and non-Saccharomyces yeasts release enzymes that are able to transform neutral compounds of grape berries into active aromatic compounds, a process that enhances the sensory attributes of wines. So far, there exists only little information about enzymatic activity in mixed cultures of Saccharomyces and non-Saccharomyces during grape must fermentations. The aim of the present work was to determine the ability of yeasts to produce extracellular enzymes of enological relevance (β-glucosidases, pectinases, proteases, amylases or xylanases) in pure and mixed Saccharomyces/non-Saccharomyces cultures during fermentation. Pure and mixed cultures of Saccharomyces cerevisiae BSc562, Hanseniaspora vinae BHv438 and Torulaspora delbrueckii BTd259 were assayed: 1% S. cerevisiae/99% H. vinae, 10% S. cerevisiae/90% H. vinae, 1% S. cerevisiae/99% T. delbrueckii and 10% S. cerevisiae/90% T. delbrueckii. Microvinifications were carried out with fresh must without pressing from Vitis vinifera L. c.v. Pedro Jiménez, an autochthonous variety from Argentina. Non-Saccharomyces species survived during 15-18days (BTd259) or until the end of the fermentation (BHv438) and influenced enzymatic profiles of mixed cultures. The results suggest that high concentrations of sugars did not affect enzymatic activity. β-Glucosidase and pectinase activities seemed to be adversely affected by an increase in ethanol: activity diminished with increasing fermentation time. Throughout the fermentation, Saccharomyces and non-Saccharomyces isolates assayed produced a broad range of enzymes of enological interest that catalyze hydrolysis of polymers present in grape juice. Vinifications carried out by a pure or mixed culture of BTd259 (99% of T. delbrueckii) showed the highest production of all enzymes assayed except for β-glucosidase. In mixed cultures, S. cerevisiae outgrew H. vinae, and T. delbrueckii was only detected until halfway the fermentation process. Nevertheless, their secreted enzymes could be detected throughout the fermentation process. Our results may contribute to a better understanding of the microbial interactions and the influence of some enzymes on vinification environments.

This paper investigates the energy system of the Basilicata region (southern Italy) to highlight, through a scenario analysis, its possible future development in compliance with the European Commission's long-term vision. The IEA-ETSAP methodology was utilized to model and analyse the Basilicata energy system in a business-as-usual scenario, based on the assumptions of the 2010 Regional Energy and Environmental Policy Plan, and in two low-carbon scenarios, decarbonisation and high energy efficiency, which, in line with the long-term European strategy, foresee an 85% carbon dioxide emission reduction and a 20% energy efficiency increase in 2050. The results highlight that electricity production from renewable energy sources (in particular wind energy), strongly supported by the regional policy, goes beyond the Basilicata Regional Energy-Environmental Plan forecast, with a further increase in the decarbonisation scenarios. In addition, solar thermal and highly efficient technologies are adopted in commercial and residential end-use sectors. An additional 9% reduction in energy consumption is achieved in the high energy efficiency scenario by passive houses. The proposed approach shows the usefulness of a modelling framework typically used for national and supranational analyses to support regional authorities in their decision-making process to obtain results in line with the European and national strategies. Application to the Basilicata region shows the usefulness of a consolidated framework for policy assessment at a local scale to assess regional contribution to the achievement of national climate mitigation targets that tend towards climate neutrality.

Conservation agriculture through no-till based on cropping systems with high biomass-C input, is a strategy to restoring the carbon (C) lost from natural capital by conversion to agricultural land. We hypothesize that cropping systems based on quantity, diversity and frequency of biomass-C input above soil C dynamic equilibrium level can recover the natural capital. The objectives of this study were to: i) assess the C-budget of land use change for two contrasting climatic environments, ii) estimate the C turnover time of the natural capital through no-till cropping systems, and iii) determine the C pathway since soil under native vegetation to no-till cropping systems. In a subtropical and tropical environment, three types of land use were used: a) undisturbed soil under native vegetation as the reference of pristine level; b) degraded soil through continuous tillage; and c) soil under continuous no-till cropping system with high biomass-C input. At the subtropical environment, the soil under continuous tillage caused loss of 25.4 Mg C ha-1 in the 0-40 cm layer over 29 years. Of this, 17 Mg C ha-1 was transferred into the 40-100 cm layers, resulting in the net negative C balance for 0-100 cm layer of 8.4 Mg C ha-1 with an environmental cost of USD 1968 ha-1. The 0.59 Mg C ha-1 yr-1 sequestration rate by no-till cropping system promote the C turnover time (soil and vegetation) of 77 years. For tropical environment, the soil C losses reached 27.0 Mg C ha-1 in the 0-100 cm layer over 8 years, with the environmental cost of USD 6155 ha-1, and the natural capital turnover time through C sequestration rate of 2.15 Mg C ha-1 yr-1 was 49 years. The results indicated that the particulate organic C and mineral associate organic C fractions are the indicators of losses and restoration of C and leading C pathway to recover natural capital through no-till cropping systems.