• Energy Research

AbstractWetlands are an important carbon sink for greenhouse gases (GHGs), and embedding microbial fuel cell (MFC) into constructed wetland (CW) has become a new technology to control methane (CH4) emission. Rhizosphere anode CW–MFC was constructed by selecting rhizome-type wetland plants with strong hypoxia tolerance, which could provide photosynthetic organics as alternative fuel. Compared with non-planted system, CH4 emission flux and power output from the planted CW–MFC increased by approximately 0.48 ± 0.02 mg/(m2·h) and 1.07 W/m3, respectively. The CH4 emission flux of the CW–MFC operated under open-circuit condition was approximately 0.46 ± 0.02 mg/(m2·h) higher than that under closed-circuit condition. The results indicated that plants contributed to the CH4 emission from the CW–MFC, especially under open-circuit mode conditions. The CH4 emission from the CW–MFC was proportional to external resistance, and it increased by 0.67 ± 0.01 mg/(m2·h) when the external resistance was adjusted from 100 to 1000 Ω. High throughput sequencing further showed that there was a competitive relationship between electrogenic bacteria and methanogens. The flora abundance of electrogenic bacteria was high, while methanogens mainly consisted of Methanothrix, Methanobacterium and Methanolinea. The form and content of element C were analysed from solid phase, liquid phase and gas phase. It was found that a large amount of carbon source (TC = 254.70 mg/L) was consumed mostly through microbial migration and conversion, and carbon storage and GHGs emission accounted for 60.38% and 35.80%, respectively. In conclusion, carbon transformation in the CW–MFC can be properly regulated via competition of microorganisms driven by environmental factors, which provides a new direction and idea for the control of CH4 emission from wetlands. Graphical Abstract

A novel ecological-microbial fuel cell (E-MFC) was constructed based on the mutualistic symbiosis relationship among wetland plants Ipomoea aquatic, benthic fauna Tubifex tubifex (T. tubifex) and microorganisms. The maximum power densities of sediment MFC (S-MFC), wetland plant MFC (WP-MFC) and E-MFC were 6.80 mW/m2, 10.60 mW/m2 and 15.59 mW/m2, respectively. Ipomoea aquatic roots secreted organic matter as electricigens' fuel for electricity generation, while T. tubifex decomposed decaying leaves and roots into soluble organic matter and plant nutrients, forming a co-dependent and mutually beneficial system, which was conducive to bioelectricity production. The E-MFC obtained the highest nitrogen removal, and the removal efficiencies of NH4+-N and NO3--N were 90.4% and 96.5%, respectively. Hydraulic retention time (HRT), cathodic aeration and T. tubifex abundance had significant effects on E-MFC power generation. The performeance boost of E-MFC was closely related to anodic microbial community change caused by the introduction of T. tubifex.

To harvest electricity and mitigate methane emissions from wetlands, a novel microbial fuel cell coupled constructed wetland (MFC-CW) was assembled with an anode placing in the rhizosphere and a cathode on the water surface. Plant-mediated methane accounted for 71-82% of the total methane fluxes. The bioanode served as an inexhaustible source of electron acceptors and resulted in reduced substantial methane emissions owing to electricigens outcompeting methanogens for carbon and electrons when substrate was deficient. However, when supplying sufficient organic carbon, both electricity and methane increased, indicating that electrogenesis and methanogenesis could co-exist in harmony. Direct methane emission (diffusion/ebullition) and plant-mediated methane emission were affected by operating conditions. Methanogenesis was significantly suppressed (∼98%) at HRT of 96h and with external resistance of 200Ω, accompanied with improved coulombic efficiency of 14.9% and current density of 187mA/m2. Contrarily, change of electrode polarity in the rhizosphere led to more methane efflux.

An innovative design of microbial electrolytic reactor (MER) coupled with Ipomoea aquaticaForsk. plant microbial fuel cell (IAF-PMFC) was developed for azo dye wastewater treatment and electricity generation. This study aims to assess the sequential degradation of azo dye and the feasibility of energy self-sufficiency in the MER/IAF-PMFC system. The decomposition of azo dye into aromatic amines and dye decolorization occurred in the MER at high hydraulic loading of 0.28 m3/(m2·d), while dye intermediates were mainly mineralized in the IAF-PMFC at low hydraulic loading of 0.06 m3/(m2·d). The final decolorization efficiency and COD removal of the combined system reached 99.64% and 92.06% respectively, even at influent dye concentration of 1000 mg/L. The effects of open/closed circuit conditions, presence/absence of aquatic plant and different cathode areas on the performance of the IAF-PMFC for treating the effluent of the MER were systematically tested, and the results showed that closed-circuit condition, plant involvement and larger cathode area were more beneficial to decolorization, detoxification and mineralization of dye wastewater, bioelectricity output, plant growth and photosynthetic rate. The power consumption by the MER was 0.0163 kWh/m3 of dye wastewater, while the highest power generation of the IAF-PMFC reached 0.0183 kWh/m3. The current efficiency of the MER for dye decolorization was as high as 942.83%, while the maximum coulombic efficiency of the IAF-PMFC for intermediates metabolism was only 6.30%, which still had much space of bioelectricity generation promotion. The MER/IAF-PMFC technology can simultaneously realize refractory wastewater treatment and balance of electricity production and consumption.