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Dried, milled perennial ryegrass samples were processed using chemical and physical treatments and the extracted cellulose products were analysed for yield, crystallinity by X-ray Diffraction (XRD) and for purity using Thermogravimetric Analysis (TGA), Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS) and Fourier Transform Infrared (FTIR) spectroscopy. Extraction protocols examined the use of chemical chelation, acid and alkaline hydrolysis, along with physical degradation methods. Highest product yields were obtained using single step chemical protocols followed by physical processing, however, these products had low crystallinity and higher amorphous fraction content. Multistep chemical processing to completely remove hemicellulose and lignin with an alkali refluxing step, delivered lower yielding cellulose products of greater crystallinity and purity. In combination, the four instrumental techniques highlighted removal of amorphous fractions, providing rapid, accurate compositional data on the extracted cellulose products.

In this study, levulinic acid (LA) was produced from rice straw biomass in co-solvent biphasic reactor system consisting of hydrochloric acid and dichloromethane organic solvent. The modified protocol achieved a 15% wt LA yield through the synergistic effect of acid and acidic products (auto-catalysis) and the designed system allowed facile recovery of LA to the organic phase. Further purification of the resulting extractant was achieved through traditional column chromatography, which yielded a high purity LA product while recovering ∼85% wt. Upon charcoal treatment of the resultant fraction generated an industrial grade target molecule of ∼99% purity with ∼95% wt recovery. The system allows the solvent to be easily recovered, in excess of 90%, which was shown to be able to be recycled up to 5 runs without significant loss of final product concentrations. Overall, this system points to a method to significantly reduce manufacturing cost during large-scale LA preparation.

Algae production process is a key cost center in production of biofuels/bioproducts from microalgae. Decline in the growth of algae in outdoor ponds during non-optimal conditions is one of the hurdles for achieving consistently high algal production rates. An optimal controller can be used to overcome this limitation and provide reliable growth in outdoor conditions. A model predictive controller (MPC) was developed to optimize the algal growth, predicted by flux balance analysis, under natural disturbances, embedding within the cost function, the economic and environmental constraints associated with the process. The model, developed in MATLAB, was validated on a 30-L continuous algal culture under light, temperature and a combination of light and temperature disturbances. The MPC proved effective in minimization of a decrease in growth under these natural disturbances. The growth rates with MPC were observed to be 79-116% higher as compared to the non-MPC growth.

The possibility of using anaerobic digestate effluent (ADE) to replace freshwater and nutrients for bioethanol production was explored. The ethanol concentration yielded from ADE and post-centrifuged ADE supernatant was 79.60±1.75 g/L and 78.33±1.66 g/L, respectively, with a 24% dry mass (DM) of soft wheat. Ethanol production was enhanced in ADE by as much as 18% in comparison to the production in freshwater (66.61±0.28 g/L, p<0.01). Without yeast nutrients, ADE fermentation yielded an ethanol concentration of 81.10±2.87 g/L, which was significantly higher than that in freshwater fermentation (59.67±1.79 g/L). Analysis showed that ADE contained rich nitrogen, proteins and minerals. After one-step distillation, the ethanol concentration attained was 700.05±46.20 g/L in ADE as compared to 622.79±32.22 g/L in freshwater (p<0.05).

To find an appropriate method for sulfate-rich wastewater containing ethanol and acetate with COD/sulfate ratio of 1, a UASB reactor was operated for more than 180 days. The influences of HRT (hydraulic retention time) and OLR (organic loading rate) on organics and sulfate removal, gas production, and electrons utilization were investigated. The sludge activity and microorganism composition were also determined. The results indicated that this system removed more than 80% of COD and 30% of sulfate with HRT above 6h and OLR below 12.3 gCOD/L d. Further HRT decrease caused volatile fatty acids accumulation and performance deterioration. Except at HRT of 2h, COD and electron flow were mostly utilized by methane-producing archaea (MPA), and methane yield remained in the range of 0.18-0.24 LCH4/gCOD. Methane was mainly generated by Methanosaeta concilii GP6 with acetate as substrate, whereas sulfate was mainly reduced by incomplete-oxidizing Desulfovibrio species with ethanol as substrate.

To fix CO2 emissions efficiently from flue gas of coal-fired power plants, the culture medium, light intensity and bioreactors were comprehensively optimized in the process of CO2 fixation by Chlorella PY-ZU1. To make up for relative insufficiency of nutrients (except for the carbon source) resulting from continuous bubbling of 15% CO2, three chemicals were added into the culture to optimize the molar ratios of nitrogen to carbon, phosphorus to carbon, and magnesium to carbon in culture from 0.17 to 0.69, from 0.093 to 0.096, and from 0.018 to 0.030, respectively. Such adjustments led to a 1.25-fold increase in biomass (from 2.41 to 5.42 g L(-1)). By enhancing light intensity from 4500 to 6000 lux, the peak growth rate of Chlorella PY-ZU1 increased by 99% and reached to 0.95 g L(-1) day(-1). Use of a multi-stage sequential bioreactor notably improved the peak CO2 fixation efficiency to 85.6%.

The objective of this work was to study the kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. The model terms glycerol concentration inoculum and C/N ratio with inoculum were found to be significant for lipid production. Lipid production was found to be higher in glycerol 82.5g/L, C/N ratio 75 and inoculum volume 6.25%. Optimized culture conditions were tested at 15L bench scale reactor. The biomass concentration and lipid content obtained was 29.5g/L and 50% (w/w), respectively. The yield coefficients were calculated and found to be 0.332g/g (g biomass/g of glycerol) of biomass and 0.179g/g (g lipid/g glycerol consumed) for lipid. Observed rates of lipid production show lipid production from 30h of fermentation. Out of the total glycerol consumed, 41.1% glycerol was converted into biomass, lipid, and citric acid.

This work studied outdoor pilot scale production of Nannochloropsis gaditana in tubular photobioreactors. The growth and biomass composition of the strain were studied under different culture strategies: continuous-mode (varying nutrient supply and dilution rate) and two-stage cultures aiming lipid enhancement. Besides, parameters such as irradiance, specific nitrate input and dilution rate were used to obtain models predicting growth, lipid and fatty acids production rates. The range of optimum dilution rate was 0.31-0.351/day with maximum biomass, lipid and fatty acids productivities of 590, 110 and 66.8 mg/l day, respectively. Nitrate limitation led to an increase in lipid and fatty acids contents (from 20.5% to 38.0% and from 16.9% to 23.5%, respectively). Two-stage culture strategy provided similar fatty acids productivities (56.4 mg/l day) but the neutral lipids content was doubled.

Rapid pyrolysis of two types of aquatic biomass (blue-green algae and water hyacinth), and their blends with two coals (bituminous and anthracite) was carried out in a high-frequency furnace. Nitrogen conversions during rapid pyrolysis of the two biomass and the interactions between the biomass and coals on nitrogen conversions were investigated. Results show that little nitrogen retained in char after the biomass pyrolysis, and NH(3) yields were higher than HCN. During co-pyrolysis of biomass and coal, interactions between biomass and coal decreased char-N yields and increased volatile-N yields, but the total yields of NH(3)+HCN in volatile-N were decreased in which HCN formations were decreased consistently, while NH(3) formations were only decreased in the high-temperature range but promoted in the low-temperature range. Interactions between blue-green algae and coals are stronger than those between water hyacinth and coal, and interactions between biomass and bituminous are stronger than those between biomass and anthracite.