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Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment
descriptionPublicationkeyboard_double_arrow_right Article , Journal 2014 Denmark Publisher:Springer Science and Business Media LLC
Authors: Ramachandran, Priyadharshini; Zhao, Zongpei; Singh, Raushan; Dhiman, Saurabh Sudha; +4 Authors
Ramachandran, Priyadharshini; Zhao, Zongpei; Singh, Raushan; Dhiman, Saurabh Sudha; Choi, Joon Ho; Kim, Dongwook; Haw, Jung Rim; Lee, Jung Kul;
doi: 10.1007/s00449-014-1156-y
pmid: 24590240
doi: 10.1007/s00449-014-1156-y
pmid: 24590240
A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chromatography on a Sephacryl gel filtration column. P. adiposa β-1,4-mannanase showed the highest activity toward locust bean gum (V max = 1,990 U/mg protein, K m = 0.12 mg/mL) ever reported. Its internal amino acid sequence showed homology with hydrolases from the glycoside hydrolase family 5 (GH5), indicating that the enzyme is a member of the GH5 family. The saccharification of commercial mannanase and P. adiposa β-1,4-mannanase-pretreated rice straw by Celluclast 1.5L (Novozymes) was compared. In comparison with the commercial Novo Mannaway(®) (113 mg/g-substrate), P. adiposa β-1,4-mannanase-pretreated rice straw released more reducing sugars (141 mg/g-substrate). These properties make P. adiposa β-1,4-mannanase a good candidate as a new commercial β-1,4-mannanase to improve biomass pretreatment.
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Article . 2014 . Peer-reviewed
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Article . 2014
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Bioprocess and Biosystems Engineering
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Bioprocess and Biosy...arrow_drop_down
Bioprocess and Biosystems Engineering
Article . 2014 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
University of Copenhagen: Research
Article . 2014
Data sources: Bielefeld Academic Search Engine (BASE)
Bioprocess and Biosystems Engineering
Article . 2015
Data sources: Europe PubMed Central
Bioprocess and Biosystems Engineering
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Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment
descriptionPublicationkeyboard_double_arrow_right Article , Journal 2014 Denmark Publisher:Springer Science and Business Media LLC
Authors: Ramachandran, Priyadharshini; Zhao, Zongpei; Singh, Raushan; Dhiman, Saurabh Sudha; +4 Authors
Ramachandran, Priyadharshini; Zhao, Zongpei; Singh, Raushan; Dhiman, Saurabh Sudha; Choi, Joon Ho; Kim, Dongwook; Haw, Jung Rim; Lee, Jung Kul;
doi: 10.1007/s00449-014-1156-y
pmid: 24590240
doi: 10.1007/s00449-014-1156-y
pmid: 24590240
A highly efficient β-1,4-mannanase-secreting strain, Pholiota adiposa SKU0714, was isolated and identified on the basis of its morphological features and sequence analysis of internal transcribed spacer rDNA. P. adiposa β-1,4-mannanase was purified to homogeneity from P. adiposa culture supernatants by one-step chromatography on a Sephacryl gel filtration column. P. adiposa β-1,4-mannanase showed the highest activity toward locust bean gum (V max = 1,990 U/mg protein, K m = 0.12 mg/mL) ever reported. Its internal amino acid sequence showed homology with hydrolases from the glycoside hydrolase family 5 (GH5), indicating that the enzyme is a member of the GH5 family. The saccharification of commercial mannanase and P. adiposa β-1,4-mannanase-pretreated rice straw by Celluclast 1.5L (Novozymes) was compared. In comparison with the commercial Novo Mannaway(®) (113 mg/g-substrate), P. adiposa β-1,4-mannanase-pretreated rice straw released more reducing sugars (141 mg/g-substrate). These properties make P. adiposa β-1,4-mannanase a good candidate as a new commercial β-1,4-mannanase to improve biomass pretreatment.
Bioprocess and Biosy...arrow_drop_down
Bioprocess and Biosystems Engineering
Article . 2014 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
University of Copenhagen: Research
Article . 2014
Data sources: Bielefeld Academic Search Engine (BASE)
Bioprocess and Biosystems Engineering
Article . 2015
Data sources: Europe PubMed Central
Bioprocess and Biosystems Engineering
Journal
Data sources: Microsoft Academic Graph
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

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This Research product is the result of merged Research products in OpenAIRE.

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10
citations 10
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Powered by BIP!
more_vert
Bioprocess and Biosy...arrow_drop_down
Bioprocess and Biosystems Engineering
Article . 2014 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
University of Copenhagen: Research
Article . 2014
Data sources: Bielefeld Academic Search Engine (BASE)
Bioprocess and Biosystems Engineering
Article . 2015
Data sources: Europe PubMed Central
Bioprocess and Biosystems Engineering
Journal
Data sources: Microsoft Academic Graph
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

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This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
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Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment

Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment

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Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment

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Characterization of a β-1,4-mannanase from a newly isolated strain of Pholiota adiposa and its application for biomass pretreatment

Energy Research
biological sciences