• Energy Research

Fire represents a major threat to Mediterranean terrestrial ecosystems because of the high temperatures reached during summer. While massive loads of organic, inorganic compounds and particulate matter are known to be emitted into the atmosphere from forest wildfires, less is known about the emission from vegetation surrounding fires where air temperatures higher than 100 °C can be reached. Little information exists on the emission from dead vegetation accumulated as litter over forest soils, from which fires often starts. In this study, the response of litter to heatwaves generated by nearby fires was investigated under controlled conditions. Litter samples collected in a Mediterranean maquis and a Holm oak stand during summer were placed in an enclosure flushed with a continuous flow of air, the temperature of the enclosure was progressively risen to 125 °C, until some smog developed but no flaming occurred. The gas from the enclosure was analysed for the content of CO2, H2O, and volatile organic compounds (VOC) to assess the dependence of emission from the air temperature. VOC emission was continuously determined by Proton-Transfer-Reaction mass spectrometry with time of flight (PTR-TOF-MS). Data obtained were complemented with those obtained by collecting VOC on traps that were later analysed by Gas chromatography-mass spectrometry (GC-MS). Results provided useful information to understand the emission mechanism of VOC and other gases from dead vegetation present in the litter of two Mediterranean ecosystems, both dominated by evergreen vegetation species. The study demonstrated that low molecular weight VOC and aromatic hydrocarbons (arenes) produced mostly by thermal oxidation of the wood biopolymers are emitted in addition to isoprenoids typically associated to storage organs and photosynthetic pathway. Moreover, our results support parameterization of litter VOC emission processes in air quality models.

To assess the effect a new animal-derived biostimulant on the growth, root morphology, nitrogen content, leaf gas exchange of greenhouse potted snapdragon, three treatments were compared: (a) three doses of biostimulant (D): 0 (D0 or control), 0.1 (D0.1), and 0.2 g L-1 (D0.2); (b) two biostimulant application methods (M): foliar spray and root drenching; (c) two F1Antirrhinum majus L. hybrids (CV): "Yellow floral showers" and "Red sonnet." The treatments were arranged in a randomized complete-block design with four replicates, with a total of 48 experimental units. Plant height (+11%), number of shoots (+20%), total shoot length (+10%), number of leaves (+33%), total leaf area (+29%), and number of flowers (+59%) and total aboveground dry weight (+13%) were significantly increased by the biostimulant application compared to the control, regardless of the dose. The lowest dose resulted in the best effect on the ground plant dry weight (+38%) and, in order to the root system, on total length (+55%), average diameter (+36%), volume (+66%), tips (+49%), crossings (+88%), forks (+68%), projected (+62%), and total surface area (+28%). Compared to the control, plants treated with the biostimulant significantly enhanced leaf (+16%) and root (+8%) nitrogen content, photosynthetic rate (+52%), transpiration rate (+55%), and stomatal conductance (+81%), although there were no changes in dark-adapted chlorophyll fluorescence. Differences in the application method were not evident in the aboveground morphological traits, except in the plant shoot number (root drenching: +10%). The foliar spray compared to root drenching had a significant effect only on flower dry weight (3.8 vs. 3.0 g plant-1). On the other hand, root drenching had a positive effect on ground dry weight (2.7 vs. 2.3 g plant-1), root morphology, leaf-N and root-N content (+3%), transpiration rate (+21%), stomatal conductance (+40%), concentration of CO2 in intracellular spaces (+11%), as well as on the efficiency of Photosystem II (+11%). A higher pot quality was obtained in "Red sonnet" compared to "Yellow floral shower." Based on our findings, applying the biostimulant to potted snapdragon at the lowest dose, as part of a fertilizing regime, improves the crop quality in an agro-environmental sustainable way.

In the Mediterranean ecosystem, wildfires are very frequent and the predicted future with a probable increase of fires could drastically modify the vegetation scenarios. Vegetation fires are an important source of gases and primary emissions of fine carbonaceous particles in the atmosphere. In this paper, we present gaseous and particulate emissions data from the combustion of different plant tissues (needles/leaves, branches and needle/leaf litter), obtained from one conifer (Pinus halepensis) and one deciduous broadleaf tree (Quercus pubescens). Both species are commonly found throughout the Mediterranean area, often subject to wildfires. Experiments were carried out in a combustion chamber continuously sampling emissions throughout the different phases of a fire (pre-ignition, flaming and smoldering). We identified and quantified 83 volatile organic compounds including important carcinogens that can affect human health. CO and CO2 were the main gaseous species emitted, benzene and toluene were the dominant aromatic hydrocarbons, methyl-vinyl-ketone and methyl-ethyl-ketone were the most abundant measured oxygenated volatile organic compounds. CO2 and methane emissions peaked during the flaming phase, while the peak of CO emissions occurred during the smoldering phase. Overall, needle/leaf combustion released a greater amount of volatile organic compounds into the atmosphere than the combustion of branches and litter. There were few differences between emissions from the combustion of the two tree species, except for some compounds. The combustion of P. halepensis released a great amount of monoterpenes as α-pinene, β-pinene, p-cymene, sabinene, 3-carene, terpinolene and camphene that are not emitted from the combustion of Q. pubescens. The combustion of branches showed the longest duration of flaming and peak of temperature. Data presented appear crucial for modeling with the intent of understanding the loss of C during different phases of fire and how different typologies of biomass can affect wildfires and their speciation emissions profile.

Norway spruce (Picea abies) is a strong emitter of biogenic volatile organic compounds (BVOCs). In the present study we investigated how shoot canopy position and high levels of stressors such as high temperature and ozone concentration, affect BVOC emission rates by means of in-situ and ex-situ experimental measurements. Therefore, BVOC emission from current-year spruce shoots was investigated under field and manipulated (temperature, ozone) laboratory conditions. Emitted BVOCs were sampled on desorption tubes, coupled with gas-exchange measurements of CO2 assimilation rate and stomatal conductance, and detected by gas chromatography coupled with mass spectrometry. Total BVOC emission rates from sun shoots under standard conditions were higher than those from shade shoots, although this was significant only in July, on the contrary, only α-pinene and γ-terpinene emission rates showed significant differences between sun and shade acclimated shoots in August. Limonene, α-pinene, β-pinene, and myrcene were identified as the most abundant BVOCs in both campaigns with emission rates above 0.2 nmol m-2 s-1. Ex-situ measurements revealed a significantly higher total BVOC emissions under high temperature level (40 °C) by ca. 175% as compared with standard temperature (30 °C), while a short-term fumigation of acute O3 concentration (200 ppb) had no effect on BVOC emissions and its spectrum. These findings might have a relevance considering the role of these compounds in protecting against oxidative stress and their possible stimulation in particular stressful conditions. Implication of such results into emission models may contribute to a more accurate estimation of BVOC emissions for Central European mountain regions dominated by Norway spruce forests and their rate under predicted climate change.