• Energy Research

These data include development (i.e. maturation) time measurements for A. tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data were calculated for naupliar development time (i.e. naupliar stage 1 to naupliar stage 6) and copepodite development time (i.e. copepodite stage 1 to adulthood).

These data include body size measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data was collected on C1 juveniles (C1), adult males (C6M), and adult females (C6F). Individual copepods were stained with non-acid lugol’s solution, isolated in a drop of filtered seawater, and photographed using a Lumenera Infinity5-5 camera (Teledyne Lumenera, Ottawa, ON, CAN) attached to an inverted microscope (Olympus IX70, Olympus, Waltham, MA, USA) after the water droplet had been removed. Body size was measured as prosome length at C1 and C6 stages using Image-J (https://imagej.nih.gov/ij/).

Excel file containing literature review citations, as well as criteria used to evaluate each study included in the review.

These data include egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data was collected as the number of eggs produced and hatched offspring per female per treatment at each generation.

These data include body size measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every second generation between F0 and F4 for all treatments and at F11 for AM and OWA. Data were collected on C1 juveniles (C1), adult males (C6M), and adult females (C6F). Individual copepods were stained with non-acid lugol's solution, isolated in a drop of filtered seawater, and photographed using a Lumenera Infinity5-5 camera (Teledyne Lumenera, Ottawa, ON, CAN) attached to an inverted microscope (Olympus IX70, Olympus, Waltham, MA, USA) after the water droplet had been removed. Body size was measured as prosome length at C1 and C6 stages using Image-J (https://imagej.nih.gov/). The experimental environmental conditions were: 1) Ambient control (AM): 13˚C, 400 µatm CO2, pH = 8.2; 2) Ocean Acidification (OA): 13˚C, 1000 µatm CO2, pH = 7.85; 3) Ocean Warming (OW): 15°C, 400 μatm CO2, pH = 8.2; 4) Combined warming and acidification (OWA): 15°C, 1000 μatm CO2, pH = 7.85.

Experimental Design. Blue paths indicate AM conditions, while red paths indicate OWA conditions. Vertical dashed lines indicate time points when experimental procedures took place.

These data include survivorship measurements collected for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data was collected as the proportion of surviving individuals on any day (x) relative to the starting number of individuals for an single experiment.

These data include survivorship measurements collected for Acartia hudsonica during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every second generation between F0 and F4 for all treatments and F11 for AM and OWA. Data were collected as the proportion of surviving individuals on any day (x) relative to the starting number of individuals for a single experiment.

Code for producing Box 2 Fig. 1.