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description Publicationkeyboard_double_arrow_right Article , Other literature type 2023 DenmarkPublisher:MDPI AG Authors:Ana Paula Maria da Silva;
Ana Paula Maria da Silva
Ana Paula Maria da Silva in OpenAIREPietro Sica;
Pietro Sica
Pietro Sica in OpenAIRELucas de Almeida Nobre Pires;
Liandra Spironello; +11 AuthorsLucas de Almeida Nobre Pires
Lucas de Almeida Nobre Pires in OpenAIREAna Paula Maria da Silva;
Ana Paula Maria da Silva
Ana Paula Maria da Silva in OpenAIREPietro Sica;
Pietro Sica
Pietro Sica in OpenAIRELucas de Almeida Nobre Pires;
Liandra Spironello;Lucas de Almeida Nobre Pires
Lucas de Almeida Nobre Pires in OpenAIRELayna Amorim Mota;
Gustavo Theodoro Peixoto;Layna Amorim Mota
Layna Amorim Mota in OpenAIRERubens Perez Calegari;
Rubens Perez Calegari
Rubens Perez Calegari in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREAldo Tonso;
Marcelo Pego Gomes; Samir Luiz Somessari; Heitor Gameiro Duarte; Elizabeth S. Ribeiro Somessari; Renan de Souza Carvalho; Antonio Sampaio Baptista;Aldo Tonso
Aldo Tonso in OpenAIRERecently, in Brazil, corn ethanol industries are being installed and the integration with sugar/energy-cane has been proposed, using bagasse for cogeneration and the juice to dilute the corn. However, this integration may have some limitations, such as the quality of the cane juice and potential contamination by microorganisms brought with the cane from the field. In this article, we first tested the effects of mixing energy cane juice with corn on fermentative parameters. We also assessed the effects of Lactobacilli. contamination on organic acids produced during the fermentation and fermentation parameters and proposed the use of ionizing radiation to replace antibiotics as a disinfection control method. Our results showed that mixing energy cane juice with corn does not have any negative effect on fermentation parameters, including ethanol production. The contamination with Lactobacilli. considerably increased the production of acetic, lactic, and succinic acid, reducing the pH and ethanol content from 89.2 g L−1 in the sterilized treatment to 72.9 g L−1 in the contaminated treatment. Therefore, for the integration between corn and cane to be applied on an industrial scale, it is essential to have effective disinfection before fermentation. Ionizing radiation (20 kGy) virtually disinfected the wort, showing itself to be a promising technology; however, an economic viability study for adopting it in the industry should be carried out.
Fermentation arrow_drop_down FermentationOther literature type . 2023License: CC BYFull-Text: http://www.mdpi.com/2311-5637/9/2/89/pdfData sources: Multidisciplinary Digital Publishing InstituteCopenhagen University Research Information SystemArticle . 2023Data sources: Copenhagen University Research Information SystemUniversity of Copenhagen: ResearchArticle . 2023Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/fermentation9020089&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 6 citations 6 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Fermentation arrow_drop_down FermentationOther literature type . 2023License: CC BYFull-Text: http://www.mdpi.com/2311-5637/9/2/89/pdfData sources: Multidisciplinary Digital Publishing InstituteCopenhagen University Research Information SystemArticle . 2023Data sources: Copenhagen University Research Information SystemUniversity of Copenhagen: ResearchArticle . 2023Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/fermentation9020089&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2023 DenmarkPublisher:MDPI AG Authors:Ana Paula Maria da Silva;
Ana Paula Maria da Silva
Ana Paula Maria da Silva in OpenAIREPietro Sica;
Pietro Sica
Pietro Sica in OpenAIRELucas de Almeida Nobre Pires;
Liandra Spironello; +11 AuthorsLucas de Almeida Nobre Pires
Lucas de Almeida Nobre Pires in OpenAIREAna Paula Maria da Silva;
Ana Paula Maria da Silva
Ana Paula Maria da Silva in OpenAIREPietro Sica;
Pietro Sica
Pietro Sica in OpenAIRELucas de Almeida Nobre Pires;
Liandra Spironello;Lucas de Almeida Nobre Pires
Lucas de Almeida Nobre Pires in OpenAIRELayna Amorim Mota;
Gustavo Theodoro Peixoto;Layna Amorim Mota
Layna Amorim Mota in OpenAIRERubens Perez Calegari;
Rubens Perez Calegari
Rubens Perez Calegari in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREAldo Tonso;
Marcelo Pego Gomes; Samir Luiz Somessari; Heitor Gameiro Duarte; Elizabeth S. Ribeiro Somessari; Renan de Souza Carvalho; Antonio Sampaio Baptista;Aldo Tonso
Aldo Tonso in OpenAIRERecently, in Brazil, corn ethanol industries are being installed and the integration with sugar/energy-cane has been proposed, using bagasse for cogeneration and the juice to dilute the corn. However, this integration may have some limitations, such as the quality of the cane juice and potential contamination by microorganisms brought with the cane from the field. In this article, we first tested the effects of mixing energy cane juice with corn on fermentative parameters. We also assessed the effects of Lactobacilli. contamination on organic acids produced during the fermentation and fermentation parameters and proposed the use of ionizing radiation to replace antibiotics as a disinfection control method. Our results showed that mixing energy cane juice with corn does not have any negative effect on fermentation parameters, including ethanol production. The contamination with Lactobacilli. considerably increased the production of acetic, lactic, and succinic acid, reducing the pH and ethanol content from 89.2 g L−1 in the sterilized treatment to 72.9 g L−1 in the contaminated treatment. Therefore, for the integration between corn and cane to be applied on an industrial scale, it is essential to have effective disinfection before fermentation. Ionizing radiation (20 kGy) virtually disinfected the wort, showing itself to be a promising technology; however, an economic viability study for adopting it in the industry should be carried out.
Fermentation arrow_drop_down FermentationOther literature type . 2023License: CC BYFull-Text: http://www.mdpi.com/2311-5637/9/2/89/pdfData sources: Multidisciplinary Digital Publishing InstituteCopenhagen University Research Information SystemArticle . 2023Data sources: Copenhagen University Research Information SystemUniversity of Copenhagen: ResearchArticle . 2023Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/fermentation9020089&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 6 citations 6 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert Fermentation arrow_drop_down FermentationOther literature type . 2023License: CC BYFull-Text: http://www.mdpi.com/2311-5637/9/2/89/pdfData sources: Multidisciplinary Digital Publishing InstituteCopenhagen University Research Information SystemArticle . 2023Data sources: Copenhagen University Research Information SystemUniversity of Copenhagen: ResearchArticle . 2023Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/fermentation9020089&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Elsevier BV Authors: Antonius J. A. van Maris;Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIRECarlos P. Silva;
Marcelo Goulart Dário; +12 AuthorsCarlos P. Silva
Carlos P. Silva in OpenAIREAntonius J. A. van Maris;Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIRECarlos P. Silva;
Marcelo Goulart Dário; Marcelo Goulart Dário;Carlos P. Silva
Carlos P. Silva in OpenAIREJean-Marc Daran;
Paulo S. Schlölg;Jean-Marc Daran
Jean-Marc Daran in OpenAIREAndreas Karoly Gombert;
Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREJúlio Cézar A. do Espirito-Santo;
Júlio Cézar A. do Espirito-Santo
Júlio Cézar A. do Espirito-Santo in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso; Stefan de Kok; Gabriela Muller;Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREBoris U. Stambuk;
Boris U. Stambuk;Boris U. Stambuk
Boris U. Stambuk in OpenAIREJack T. Pronk;
Jack T. Pronk
Jack T. Pronk in OpenAIREpmid: 21963484
Sucrose is a major carbon source for industrial bioethanol production by Saccharomyces cerevisiae. In yeasts, two modes of sucrose metabolism occur: (i) extracellular hydrolysis by invertase, followed by uptake and metabolism of glucose and fructose, and (ii) uptake via sucrose-proton symport followed by intracellular hydrolysis and metabolism. Although alternative start codons in the SUC2 gene enable synthesis of extracellular and intracellular invertase isoforms, sucrose hydrolysis in S. cerevisiae predominantly occurs extracellularly. In anaerobic cultures, intracellular hydrolysis theoretically enables a 9% higher ethanol yield than extracellular hydrolysis, due to energy costs of sucrose-proton symport. This prediction was tested by engineering the promoter and 5' coding sequences of SUC2, resulting in predominant (94%) cytosolic localization of invertase. In anaerobic sucrose-limited chemostats, this iSUC2-strain showed an only 4% increased ethanol yield and high residual sucrose concentrations indicated suboptimal sucrose-transport kinetics. To improve sucrose-uptake affinity, it was subjected to 90 generations of laboratory evolution in anaerobic, sucrose-limited chemostat cultivation, resulting in a 20-fold decrease of residual sucrose concentrations and a 10-fold increase of the sucrose-transport capacity. A single-cell isolate showed an 11% higher ethanol yield on sucrose in chemostat cultures than an isogenic SUC2 reference strain, while transcriptome analysis revealed elevated expression of AGT1, encoding a disaccharide-proton symporter, and other maltose-related genes. After deletion of both copies of the duplicated AGT1, growth characteristics reverted to that of the unevolved SUC2 and iSUC2 strains. This study demonstrates that engineering the topology of sucrose metabolism is an attractive strategy to improve ethanol yields in industrial processes.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.ymben.2011.09.005&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu93 citations 93 popularity Top 1% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.ymben.2011.09.005&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Elsevier BV Authors: Antonius J. A. van Maris;Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIRECarlos P. Silva;
Marcelo Goulart Dário; +12 AuthorsCarlos P. Silva
Carlos P. Silva in OpenAIREAntonius J. A. van Maris;Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIRECarlos P. Silva;
Marcelo Goulart Dário; Marcelo Goulart Dário;Carlos P. Silva
Carlos P. Silva in OpenAIREJean-Marc Daran;
Paulo S. Schlölg;Jean-Marc Daran
Jean-Marc Daran in OpenAIREAndreas Karoly Gombert;
Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREJúlio Cézar A. do Espirito-Santo;
Júlio Cézar A. do Espirito-Santo
Júlio Cézar A. do Espirito-Santo in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso; Stefan de Kok; Gabriela Muller;Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREBoris U. Stambuk;
Boris U. Stambuk;Boris U. Stambuk
Boris U. Stambuk in OpenAIREJack T. Pronk;
Jack T. Pronk
Jack T. Pronk in OpenAIREpmid: 21963484
Sucrose is a major carbon source for industrial bioethanol production by Saccharomyces cerevisiae. In yeasts, two modes of sucrose metabolism occur: (i) extracellular hydrolysis by invertase, followed by uptake and metabolism of glucose and fructose, and (ii) uptake via sucrose-proton symport followed by intracellular hydrolysis and metabolism. Although alternative start codons in the SUC2 gene enable synthesis of extracellular and intracellular invertase isoforms, sucrose hydrolysis in S. cerevisiae predominantly occurs extracellularly. In anaerobic cultures, intracellular hydrolysis theoretically enables a 9% higher ethanol yield than extracellular hydrolysis, due to energy costs of sucrose-proton symport. This prediction was tested by engineering the promoter and 5' coding sequences of SUC2, resulting in predominant (94%) cytosolic localization of invertase. In anaerobic sucrose-limited chemostats, this iSUC2-strain showed an only 4% increased ethanol yield and high residual sucrose concentrations indicated suboptimal sucrose-transport kinetics. To improve sucrose-uptake affinity, it was subjected to 90 generations of laboratory evolution in anaerobic, sucrose-limited chemostat cultivation, resulting in a 20-fold decrease of residual sucrose concentrations and a 10-fold increase of the sucrose-transport capacity. A single-cell isolate showed an 11% higher ethanol yield on sucrose in chemostat cultures than an isogenic SUC2 reference strain, while transcriptome analysis revealed elevated expression of AGT1, encoding a disaccharide-proton symporter, and other maltose-related genes. After deletion of both copies of the duplicated AGT1, growth characteristics reverted to that of the unevolved SUC2 and iSUC2 strains. This study demonstrates that engineering the topology of sucrose metabolism is an attractive strategy to improve ethanol yields in industrial processes.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.ymben.2011.09.005&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu93 citations 93 popularity Top 1% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.ymben.2011.09.005&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010Publisher:Springer Science and Business Media LLC Authors:Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIREAndreas Karoly Gombert;
Marcelo Goulart Dário;Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREThiago Olitta Basso;
+1 AuthorsThiago Olitta Basso
Thiago Olitta Basso in OpenAIREAldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIREAndreas Karoly Gombert;
Marcelo Goulart Dário;Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREBoris U. Stambuk;
Boris U. Stambuk
Boris U. Stambuk in OpenAIREpmid: 20349336
A combination of chemostat cultivation and a defined medium was used to demonstrate that uracil limitation leads to a drastic alteration in the physiology of auxotrophic cells of Saccharomyces cerevisiae. Under this condition, the carbon source is dissimilated mainly to ethanol and acetate, even in fully aerobic cultures grown at 0.1 h(-1), which is far below the critical dilution rate. Differently from nitrogen-, sulphur-, or phosphate-limited cultures, uracil limitation leads to residual sugar (either glucose or sucrose) concentrations below 2 mM, which characterizes a situation of double-limitation: by the carbon source and by uracil. Furthermore, the specific rates of CO2 production and O2 consumption are increased when compared to the corresponding prototrophic strain. We conclude that when auxotrophic strains are to be used for quantitative physiological studies, special attention must be paid to the cultivation conditions, mainly regarding medium formulation, in order to avoid limitation of growth by the auxotrophic nutrient.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s10529-010-0248-2&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu17 citations 17 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s10529-010-0248-2&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010Publisher:Springer Science and Business Media LLC Authors:Aldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIREAndreas Karoly Gombert;
Marcelo Goulart Dário;Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREThiago Olitta Basso;
+1 AuthorsThiago Olitta Basso
Thiago Olitta Basso in OpenAIREAldo Tonso;
Aldo Tonso
Aldo Tonso in OpenAIREAndreas Karoly Gombert;
Marcelo Goulart Dário;Andreas Karoly Gombert
Andreas Karoly Gombert in OpenAIREThiago Olitta Basso;
Thiago Olitta Basso
Thiago Olitta Basso in OpenAIREBoris U. Stambuk;
Boris U. Stambuk
Boris U. Stambuk in OpenAIREpmid: 20349336
A combination of chemostat cultivation and a defined medium was used to demonstrate that uracil limitation leads to a drastic alteration in the physiology of auxotrophic cells of Saccharomyces cerevisiae. Under this condition, the carbon source is dissimilated mainly to ethanol and acetate, even in fully aerobic cultures grown at 0.1 h(-1), which is far below the critical dilution rate. Differently from nitrogen-, sulphur-, or phosphate-limited cultures, uracil limitation leads to residual sugar (either glucose or sucrose) concentrations below 2 mM, which characterizes a situation of double-limitation: by the carbon source and by uracil. Furthermore, the specific rates of CO2 production and O2 consumption are increased when compared to the corresponding prototrophic strain. We conclude that when auxotrophic strains are to be used for quantitative physiological studies, special attention must be paid to the cultivation conditions, mainly regarding medium formulation, in order to avoid limitation of growth by the auxotrophic nutrient.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s10529-010-0248-2&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu17 citations 17 popularity Average influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s10529-010-0248-2&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2020 BrazilPublisher:Informa UK Limited Authors: Rominne Karla Barros Freire; Carlos Miguel Nóbrega Mendonça; Rafael Bertelli Ferraro;Ignacio Sánchez Moguel;
+5 AuthorsIgnacio Sánchez Moguel
Ignacio Sánchez Moguel in OpenAIRERominne Karla Barros Freire; Carlos Miguel Nóbrega Mendonça; Rafael Bertelli Ferraro;Ignacio Sánchez Moguel;
Ignacio Sánchez Moguel
Ignacio Sánchez Moguel in OpenAIREAldo Tonso;
Felipe Rebello Lourenço; João Henrique Picado Madalena Santos; Lara Durães Sette;Aldo Tonso
Aldo Tonso in OpenAIREAdalberto Pessoa Junior;
Adalberto Pessoa Junior
Adalberto Pessoa Junior in OpenAIREL-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L-1) and volumetric productivity (5.12 U.L-1 h-1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L-1 sucrose, 15 g L-1 yeast extract and 20 g L-1 proline, which resulted in 4582.5 U L-1 and 63.64 U L-1 h-1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12-15 °C, pH 5.5-6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1080/10826068.2020.1815053&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen 19 citations 19 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1080/10826068.2020.1815053&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2020 BrazilPublisher:Informa UK Limited Authors: Rominne Karla Barros Freire; Carlos Miguel Nóbrega Mendonça; Rafael Bertelli Ferraro;Ignacio Sánchez Moguel;
+5 AuthorsIgnacio Sánchez Moguel
Ignacio Sánchez Moguel in OpenAIRERominne Karla Barros Freire; Carlos Miguel Nóbrega Mendonça; Rafael Bertelli Ferraro;Ignacio Sánchez Moguel;
Ignacio Sánchez Moguel
Ignacio Sánchez Moguel in OpenAIREAldo Tonso;
Felipe Rebello Lourenço; João Henrique Picado Madalena Santos; Lara Durães Sette;Aldo Tonso
Aldo Tonso in OpenAIREAdalberto Pessoa Junior;
Adalberto Pessoa Junior
Adalberto Pessoa Junior in OpenAIREL-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L-1) and volumetric productivity (5.12 U.L-1 h-1). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L-1 sucrose, 15 g L-1 yeast extract and 20 g L-1 proline, which resulted in 4582.5 U L-1 and 63.64 U L-1 h-1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12-15 °C, pH 5.5-6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes.
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