• Energy Research

The aim of the study was to determine the biogas productivity from selected cultivars of sorghum (Sorghum bicolor Moench) and maize (Zea mays L.) depending on the dose of mineral fertilization with nitrogen. The topic is novelty in the northern Poland area due to the fact that this crop is not very widespread here. The silage samples were derived from two experiments: (1) two factors experiment with sorghum varieties (Arbatax, KWS Maja, Herkules) and two doses of mineral nitrogen fertilization (0 and 150 kg ha-1 N) in split-plot design. (2) one-factor experiment with fodder maize, variety NK Magitop, and two doses of mineral nitrogen fertilization (0 and 150 kg ha-1 N) in a randomized complete block design. The experiment was performed in four replications in the split-plot design. Methane fermentation was carried out under mesophilic conditions. The temperature of the process was 37°C ± 1°C, while pH 7 ± 0.1. The content of total solids in the bioreactor was 7.0%. The composition of the gas produced was measured once a day with the use of an automatic biogas analyser (GFM 416, GasData). The trial was run in triplicate until the daily yield was less than 1% of the cumulative biogas yield [DIN 38 414-S8. Sediments and sediments. Determination of fermentation characteristics; 1985]. Sorghum was characterized by higher average biogas productivity (about 12%), higher methane content in biogas (about 10%), and higher methane productivity (about 43%). It can, therefore, be stated that sorghum represents as an alternative plant to maize for the purpose of biogas production.

The aim of this work was to analyze the impact of three different moisture contents (MC), at 45% MC, 65% MC, 75% MC, on the degradation of cellulose, hemicellulose, and lignin during fungi treatment by Flammulina velutipes of Agropyron elongatum 'BAMAR' and on biogas production. The analysis of chemical composition shown that F. velutipes had greater selectivity for lignin biodegradation with the highest hemicellulose and lignin removal at 29.1% and 35.4%, respectively, and lowest cellulose removal (20.48%) at 65% MC. F. velutipes cultivated at 65% MC increased biogas production of 398.07Ndm(3)kg(-1)VS(-1), which was 120% higher than the untreated sample. These treatment conditions resulted in 134% more methane yield compared with untreated sample. The results of this study suggested that A. elongatum is a potential biomass for biogas production in agriculture biogas plant and white-rot fungus F. velutipes provides an effective methods for improve biodegradation of A. elongatum.

In this study fungal pretreatment of non-sterile tall wheat grass via the white rot fungi Flammulina velutipes was studied and the effect on biodegradability of lignocellulosic biomass and methane production, was evaluated. Degradation of lignin, cellulose, hemicellulose, and dry matter in non-sterile tall wheat grass during 28 days of fungal pretreatment using different inoculum ratio (0%-50%) and moisture content (MC) (45% MC, 65% MC, and 75% MC) were assessed via comparison to untreated biomass. Pretreatment with F. velutipes was most effective at 65% MC and 40% inoculum ratio, resulting in 22% lignin removal. The corresponding methane yields were 181.3 Ndm3·kg VS-1, which were 280% higher than for the untreated tall wheat grass.

The aim of this study was to develop a methodology to investigate the biofermentation process in small-volume fermenters. Dark serum bottles with a volume of 100–120 mL, tightly sealed with a rubber septum, were used as bioreactors. The optimum measurement conditions in this type of bioreactor comprise: (i) filling two-thirds of the maximum volume with a suspension; (ii) a 2% bioreactor loading (on a dry basis) and; (iii) the daily equalization of pressure by removing the biogas through the septum pierced with a syringe needle and the intensive mixing of the remaining suspension. The methane yield (quantity and dynamics) obtained in this type of bioreactor is analogous to that of industrial bioreactors or large-scale laboratory bioreactors. The use of small-volume bioreactors that can be incubated will facilitate the preliminary selection of analysed systems and provide an indication of those that should be investigated in large-scale bioreactors.