• Energy Research

Pentose-rich hydrolysate obtained from dilute acid pretreatment of oil palm empty fruit bunches was successfully consumed by pentose-consuming yeasts: Cyberlindnera jadinii (Cj) and Pichia jadinii (Pj). Nitrogen supplementation and no additional detoxification step were required. Pj produced 5.87 g/L of biomass using a C/N ratio of 14 after 120 h of fermentation, with xylose consumption of 71%. Cj produced 10.50 g/L of biomass after 96 h of fermentation with C/N ratio of 11.5, with maximum xylose consumption of 85%. β-glucans, high value-added macromolecules, were further extracted from the yeast biomass, achieving yields of 3.1 and 3.0% from Pj and Cj, respectively. The isolated polysaccharides showed a chemical structure of β-(1,3)-glucan with residues of other molecules. Additionally, β-(1,6) branches seems to have been broken during isolation process. Further studies assessing β-glucans production at industrial scale should be carried out looking for nitrogen sources and optimizing the β-glucan isolation method.

Pentose-rich hydrolysate obtained from dilute acid pretreatment of oil palm empty fruit bunches was successfully consumed by pentose-consuming yeasts: Cyberlindnera jadinii (Cj) and Pichia jadinii (Pj). Nitrogen supplementation and no additional detoxification step were required. Pj produced 5.87 g/L of biomass using a C/N ratio of 14 after 120 h of fermentation, with xylose consumption of 71%. Cj produced 10.50 g/L of biomass after 96 h of fermentation with C/N ratio of 11.5, with maximum xylose consumption of 85%. β-glucans, high value-added macromolecules, were further extracted from the yeast biomass, achieving yields of 3.1 and 3.0% from Pj and Cj, respectively. The isolated polysaccharides showed a chemical structure of β-(1,3)-glucan with residues of other molecules. Additionally, β-(1,6) branches seems to have been broken during isolation process. Further studies assessing β-glucans production at industrial scale should be carried out looking for nitrogen sources and optimizing the β-glucan isolation method.

Lignin is an important raw material for the sustainable biorefineries and also the forerunner of high-value added products, such as biocomposite for chemical, pharmaceutical and cement industries. Oil palm empty fruit bunches (OPEFB) were used for lignin preparation by successive treatment with 1% (w/w) H2SO4 at 121°C for 60 min and 2.5% NaOH at 121°C for 80 min resulting in the high lignin yield of 28.89%, corresponding to 68.82% of the original lignin. The lignin obtained was characterized by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). The results indicated a lignin with molecular masses ramping from 4500 kDa to 12,580 kDa. FTIR and NMR of these lignins showed more syringyl and p-hydroxyphenyl than guaiacyl units. Moderate acid/alkaline treatment provided lignin with high industrial potential and acid hydrolyzates rich in fermentable sugars and highly porous cellulosic fibers.

Lignin is an important raw material for the sustainable biorefineries and also the forerunner of high-value added products, such as biocomposite for chemical, pharmaceutical and cement industries. Oil palm empty fruit bunches (OPEFB) were used for lignin preparation by successive treatment with 1% (w/w) H2SO4 at 121°C for 60 min and 2.5% NaOH at 121°C for 80 min resulting in the high lignin yield of 28.89%, corresponding to 68.82% of the original lignin. The lignin obtained was characterized by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). The results indicated a lignin with molecular masses ramping from 4500 kDa to 12,580 kDa. FTIR and NMR of these lignins showed more syringyl and p-hydroxyphenyl than guaiacyl units. Moderate acid/alkaline treatment provided lignin with high industrial potential and acid hydrolyzates rich in fermentable sugars and highly porous cellulosic fibers.

The aim of the work was to study the production of the exopolysaccharides by Agaricus brasiliensis and the isolation of exopolysaccharides (EPSs) with biological effects. A brasiliensis LPB03 was cultured in submerged fermentation in a medium containing glucose, yeast extract, hydrolyzed soybean protein, and salts (pH 6.1) at 29 degrees C and 120 rpm for 144 h. The maximum biomass and EPS yield was 7.80 +/- 0.01 and 1,430.70 +/- 26.75 mg/L, respectively. To isolate the produced EPSs, two methods were compared: (1) with alcohol precipitation and (2) treatment with tricloroacetic acid (TCA), followed by alcohol precipitation. The use of TCA facilitated the purification of the EPS, reducing the amount of the contaminant soy proteins. For monosaccharide identification, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry, which showed the presence (in molar percentage) of mannose (58.7), galactose (21.4), and glucose (13.1) as major sugars, with lower amounts of rhamnose (3.9) and xylose (2.8). Scanning electron microscopy was used to observe the morphological structure of the EPS. The experiments in vivo including EPS in the mice diet during 8 weeks indicated the hipocholesteremic and hypoglycemic effects.

The aim of the work was to study the production of the exopolysaccharides by Agaricus brasiliensis and the isolation of exopolysaccharides (EPSs) with biological effects. A brasiliensis LPB03 was cultured in submerged fermentation in a medium containing glucose, yeast extract, hydrolyzed soybean protein, and salts (pH 6.1) at 29 degrees C and 120 rpm for 144 h. The maximum biomass and EPS yield was 7.80 +/- 0.01 and 1,430.70 +/- 26.75 mg/L, respectively. To isolate the produced EPSs, two methods were compared: (1) with alcohol precipitation and (2) treatment with tricloroacetic acid (TCA), followed by alcohol precipitation. The use of TCA facilitated the purification of the EPS, reducing the amount of the contaminant soy proteins. For monosaccharide identification, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry, which showed the presence (in molar percentage) of mannose (58.7), galactose (21.4), and glucose (13.1) as major sugars, with lower amounts of rhamnose (3.9) and xylose (2.8). Scanning electron microscopy was used to observe the morphological structure of the EPS. The experiments in vivo including EPS in the mice diet during 8 weeks indicated the hipocholesteremic and hypoglycemic effects.

The development of new strategies in microalgal studies represents an outstanding opportunity to mitigate environmental problems coupled with biomass production at a reduced cost. Here we present a combined bioprocess for the treatment of rice vinasse using immobilized cyanobacteria Synechococcus pevalekii in alginate beads followed by the use of the treated vinasse as a culture medium for Dunaliella salina biomass production. Cyanobacterial-alginate beads showed a chlorophyll a production of 0.68 × 10-3 mg bead-1 and a total carotenoid production of 0.64 × 10-3 mg bead-1. The first step showed a decrease in nitrate (91%), total solids (29%), and ions. Addition of treated vinasse into D. salina cultivation resulted in a significant increase in cell replication of about 175% (optimized cultivation). The use of natural seawater drastically reduced the medium cost to US$4.75 per m3 and the addition of treated vinasse has the potential to reduce it even more (up to 69%). This study not only provides an insight on the use of cyanobacteria for rice vinasse treatment but also demonstrates a promising lower-cost medium for marine microalgal biomass production with biotechnological purposes.

The development of new strategies in microalgal studies represents an outstanding opportunity to mitigate environmental problems coupled with biomass production at a reduced cost. Here we present a combined bioprocess for the treatment of rice vinasse using immobilized cyanobacteria Synechococcus pevalekii in alginate beads followed by the use of the treated vinasse as a culture medium for Dunaliella salina biomass production. Cyanobacterial-alginate beads showed a chlorophyll a production of 0.68 × 10-3 mg bead-1 and a total carotenoid production of 0.64 × 10-3 mg bead-1. The first step showed a decrease in nitrate (91%), total solids (29%), and ions. Addition of treated vinasse into D. salina cultivation resulted in a significant increase in cell replication of about 175% (optimized cultivation). The use of natural seawater drastically reduced the medium cost to US$4.75 per m3 and the addition of treated vinasse has the potential to reduce it even more (up to 69%). This study not only provides an insight on the use of cyanobacteria for rice vinasse treatment but also demonstrates a promising lower-cost medium for marine microalgal biomass production with biotechnological purposes.

Microalgae have been shown to be useful in several biotechnological fields due to their feasible cultivation and high-value biomolecules production. Several substances of interest produced by microalgae, such as: proteins, lipids, and natural colorants, have already been explored. Based on the continuing demand for new natural molecules, microalgae could also be a valuable source of polysaccharides. Polysaccharides are extremely important in aquaculture, cosmetics, pharmaceutical, and food industries, and have great economic impact worldwide. Despite this, reviews on microalgal polysaccharide production, biological activity, and chemical structure are not abundant. Moreover, techniques of microalgal cultivation, coupled with carbohydrate production, need to be clarified in order to develop forward-looking technologies. The present review provides an overview of the main advances in microalgal cell wall polysaccharide production, as well as their associated potential biological applications and chemical structure. Several studies on future prospects, related to microalgae are presented, highlighting the key challenges in microalgal polysaccharide production.