• Energy Research

Abstract A process engineering strategy was developed for cultivation of high density biomass of Chlorella sp. FC2 with improved productivity under photoautotrophic condition. The process engineering strategy involved a combinatorial approach of: (i) optimization of CO2 concentration in the inlet gas stream & aeration rate; (ii) growth kinetic driven feeding recipe for limiting nutrients; and (iii) dynamic increase in light intensity. The strategy was tested by growing the cells on laboratory grade BG11 medium. With an attempt to reduce the cultivation cost, the growth performance of the organism was then evaluated on commercial grade BG11 medium. Finally, hydrothermal liquefaction was carried out for direct conversion of microalgal slurry into bio-crude oil. Cultivation on laboratory grade BG11 medium resulted in biomass titer and overall productivity of 8.41 g L−1 and 575.9 mg L−1 day−1 respectively. Significant improvement in biomass titer (13.23 g L−1) and overall productivity (731.6 mg L−1 day−1) was observed when grown on commercial grade BG11 medium. Higher fraction of hydrocarbon in the bio-crude oil depicted better oil quality. Thermal gravimetric analysis revealed that maximum distillate fraction lies within the boiling point range of 200–300 °C which is suitable for conversion into diesel oil, jet fuel, and fuel for stoves.

A novel CO2 tolerant microalga Tetradesmus obliquus CT02, was previously evaluated to be a suitable bio refinery platform for synthesis of bioactive molecules, biodiesel, and biofertilizer. In the present study, a process engineering strategy was developed targeting improved growth performance of the strain at large scale under fluctuating outdoor environmental conditions. The strategy relies on maintaining pH of the culture at its optimal value via cascade control with CO2 feeding. The strategy was developed at laboratory scale bubble column photobioreactor under diurnal variation of simulated sunlight intensity and was further validated through growth performance of the strain under outdoor conditions in a 100 L airlift bioreactor. Under laboratory condition, 53.3% and 85.16% improvement in biomass concentration (1.87 g L-1) and productivity (114.8 mg L-1 day-1) was achieved as compared to the uncontrolled pH, respectively. The strategy demonstrated a significant improvement in biomass concentration and productivity by 225.7% and 121.6% respectively, compared to the pH uncontrolled batch, even under outdoor fluctuating environmental condition.