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description Publicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Elsevier BV Steven Koenigsberg; Mark E. Conrad; Carlos Rios-Velazquez; Ramon E. Martinez; Boris Faybishenko; Josue Malave; Eoin L. Brodie; Terry C. Hazen; Anna Willett; Donald J. Herman; Benjamin V. Mork; Mary K. Firestone; Dominique C. Joyner;pmid: 21872904
To evaluate the efficacy of bioimmobilization of Cr(VI) in groundwater at the Department of Energy Hanford site, we conducted a series of microcosm experiments using a range of commercial electron donors with varying degrees of lactate polymerization (polylactate). These experiments were conducted using Hanford Formation sediments (coarse sand and gravel) immersed in Hanford groundwater, which were amended with Cr(VI) and several types of lactate-based electron donors (Hydrogen Release Compound, HRC; primer-HRC, pHRC; extended release HRC) and the polylactate-cysteine form (Metal Remediation Compound, MRC). The results showed that polylactate compounds stimulated an increase in bacterial biomass and activity to a greater extent than sodium lactate when applied at equivalent carbon concentrations. At the same time, concentrations of headspace hydrogen and methane increased and correlated with changes in the microbial community structure. Enrichment of Pseudomonas spp. occurred with all lactate additions, and enrichment of sulfate-reducing Desulfosporosinus spp. occurred with almost complete sulfate reduction. The results of these experiments demonstrate that amendment with the pHRC and MRC forms result in effective removal of Cr(VI) from solution most likely by both direct (enzymatic) and indirect (microbially generated reductant) mechanisms.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2011.07.021&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu51 citations 51 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2011.07.021&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2017 United StatesPublisher:Wiley Alex J. Lewis; Alex J. Lewis; Maria Fernanda Campa; Terry C. Hazen; Abhijeet P. Borole;SummaryLeveraging nature's biocomplexity for solving human problems requires better understanding of the syntrophic relationships in engineered microbiomes developed in bioreactor systems. Understanding the interactions between microbial players within the community will be key to enhancing conversion and production rates from biomass streams. Here we investigate a bioelectrochemical system employing an enriched microbial consortium for conversion of a switchgrass‐derived bio‐oil aqueous phase (BOAP) into hydrogen via microbial electrolysis (MEC). MECs offer the potential to produce hydrogen in an integrated fashion in biorefinery platforms and as a means of energy storage through decentralized production to supply hydrogen to fuelling stations, as the world strives to move towards cleaner fuels and electricity‐mediated transportation. A unique approach combining differential substrate and redox conditions revealed efficient but rate‐limiting fermentation of the compounds within BOAP by the anode microbial community through a division of labour strategy combined with multiple levels of syntrophy. Despite the fermentation limitation, the adapted abilities of the microbial community resulted in a high hydrogen productivity of 9.35 L per L‐day. Using pure acetic acid as the substrate instead of the biomass‐derived stream resulted in a three‐fold improvement in productivity. This high rate of exoelectrogenesis signifies the potential commercial feasibility of MEC technology for integration in biorefineries.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2018Full-Text: https://escholarship.org/uc/item/9fg7h8z9Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1111/1751-7915.12756&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 26 citations 26 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2018Full-Text: https://escholarship.org/uc/item/9fg7h8z9Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1111/1751-7915.12756&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2011 AustraliaPublisher:Royal Society of Chemistry (RSC) April Wong; April Wong; Wolfgang Reindl; Gang Cheng; Gang Cheng; Seema Singh; Seema Singh; Terry C. Hazen; C.-H. Yao; Trent R. Northen; Kai Deng; Kai Deng; Anup K. Singh; Anup K. Singh; J.-C. Lee; Steven W. Singer; John M. Gladden; John M. Gladden; Blake A. Simmons; Blake A. Simmons; Paul D. Adams;doi: 10.1039/c1ee01112j
The enzymatic hydrolysis of polysaccharides into fermentable sugars is a crucial step in the conversion of biomass to lignocellulosic biofuels. An efficient hydrolysis is highly dependent on the identification and characterization of optimal glycoside hydrolases. However, existing techniques for characterizing activity are limited by the range of reaction conditions that can be used, sample complexity, and throughput. The method we present is a multiplexed approach based on nanostructure-initiator mass spectrometry (NIMS) that allows for the rapid analysis of several glycolytic activities in parallel under diverse assay conditions. By forming colloids, it was possible to perform aqueous reactions in tubes and microwell plates despite the substrate analogs' hydrophobic perfluorinated tags. This method was validated by analyzing standard enzymatic parameters (temperature, pH, and kinetics) of β-glucosidase and β-xylosidase in separate setups. The multiplexity of this assay system was demonstrated by the simultaneous analysis of β-glucosidase and β-xylosidase activities, which was then used to profile environmental samples. Enzymes secreted by microbial communities within these samples were extracted by washing the sample with buffer. Enzymatic activities were directly detected within this crude extract without any further sample pretreatment steps. The multiplexed analysis of β-glucosidase, exo-/endoglucanase, and xylanase activities was applied for a detailed characterization of previously unknown glycoside hydrolase activities. The results show the suitability of the described method for the rapid screening of crude environmental samples under a wide range of conditions to determine enzyme activities from the microbial communities present within these samples.
Energy & Environment... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/c1ee01112j&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Energy & Environment... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/c1ee01112j&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2008 United StatesPublisher:Wiley Samuel A. Myers; Terry C. Hazen; Harvey W. Blanch; Harvey W. Blanch; Harvey W. Blanch; David S. Reichmuth; Dominique C. Joyner; Rajat Sapra; Rajat Sapra; Yinjie J. Tang; Jay D. Keasling;doi: 10.1002/bit.22181
pmid: 19016470
AbstractA recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C‐based flux analysis to provide insights into the physiological properties of this extremophile and explore its metabolism for bio‐ethanol or other bioprocess applications. Our findings show that glucose metabolism in G. thermoglucosidasius M10EXG proceeds via glycolysis, the pentose phosphate pathway, and the TCA cycle; the Entner–Doudoroff pathway and transhydrogenase activity were not detected. Anaplerotic reactions (including the glyoxylate shunt, pyruvate carboxylase, and phosphoenolpyruvate carboxykinase) were active, but fluxes through those pathways could not be accurately determined using amino acid labeling. When growth conditions were switched from aerobic to micro‐aerobic conditions, fluxes (based on a normalized glucose uptake rate of 100 units (g DCW)−1 h−1) through the TCA cycle and oxidative pentose phosphate pathway were reduced from 64 ± 3 to 25 ± 2 and from 30 ± 2 to 19 ± 2, respectively. The carbon flux under micro‐aerobic growth was directed to ethanol, L‐lactate (>99% optical purity), acetate, and formate. Under fully anerobic conditions, G. thermoglucosidasius M10EXG used a mixed acid fermentation process and exhibited a maximum ethanol yield of 0.38 ± 0.07 mol mol−1 glucose. In silico flux balance modeling demonstrates that lactate and acetate production from G. thermoglucosidasius M10EXG reduces the maximum ethanol yield by approximately threefold, thus indicating that both pathways should be modified to maximize ethanol production. Biotechnol. Bioeng. 2009;102: 1377–1386. © 2008 Wiley Periodicals, Inc.
Biotechnology and Bi... arrow_drop_down Biotechnology and BioengineeringArticle . 2008 . Peer-reviewedLicense: Wiley Online Library User AgreementData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/bit.22181&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 58 citations 58 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Biotechnology and Bi... arrow_drop_down Biotechnology and BioengineeringArticle . 2008 . Peer-reviewedLicense: Wiley Online Library User AgreementData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/bit.22181&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010Publisher:American Society for Microbiology Kathryne G. Byrne-Bailey; Ryan A. Melnyk; Terry C. Hazen; John D. Coates; Peter Agbo; Kelly C. Wrighton;ABSTRACT “ Thermincola potens ” strain JR is one of the first Gram-positive dissimilatory metal-reducing bacteria (DMRB) for which there is a complete genome sequence. Consistent with the physiology of this organism, preliminary annotation revealed an abundance of multiheme c -type cytochromes that are putatively associated with the periplasm and cell surface in a Gram-positive bacterium. Here we report the complete genome sequence of strain JR.
Journal of Bacteriol... arrow_drop_down Journal of BacteriologyArticle . 2010 . Peer-reviewedLicense: ASM Journals Non-Commercial TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1128/jb.00044-10&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 39 citations 39 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Journal of Bacteriol... arrow_drop_down Journal of BacteriologyArticle . 2010 . Peer-reviewedLicense: ASM Journals Non-Commercial TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1128/jb.00044-10&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010 Australia, United States, United States, United StatesPublisher:Springer Science and Business Media LLC Whendee L. Silver; Whendee L. Silver; Julian L. Fortney; Julian L. Fortney; Blake A. Simmons; Blake A. Simmons; Jean S. Vander Gheynst; Jean S. Vander Gheynst; Amitha M. Reddy; Amitha M. Reddy; Patrik D'haeseleer; Patrik D'haeseleer; Philip Hugenholtz; Philip Hugenholtz; Kristen M. DeAngelis; Kristen M. DeAngelis; Terry C. Hazen; Terry C. Hazen; Steven W. Singer; Steven W. Singer; John M. Gladden; John M. Gladden; Martin Allgaier; Martin Allgaier;Producing cellulosic biofuels from plant material has recently emerged as a key US Department of Energy goal. For this technology to be commercially viable on a large scale, it is critical to make production cost efficient by streamlining both the deconstruction of lignocellulosic biomass and fuel production. Many natural ecosystems efficiently degrade lignocellulosic biomass and harbor enzymes that, when identified, could be used to increase the efficiency of commercial biomass deconstruction. However, ecosystems most likely to yield relevant enzymes, such as tropical rain forest soil in Puerto Rico, are often too complex for enzyme discovery using current metagenomic sequencing technologies. One potential strategy to overcome this problem is to selectively cultivate the microbial communities from these complex ecosystems on biomass under defined conditions, generating less complex biomass-degrading microbial populations. To test this premise, we cultivated microbes from Puerto Rican soil or green waste compost under precisely defined conditions in the presence dried ground switchgrass (Panicum virgatum L.) or lignin, respectively, as the sole carbon source. Phylogenetic profiling of the two feedstock-adapted communities using SSU rRNA gene amplicon pyrosequencing or phylogenetic microarray analysis revealed that the adapted communities were significantly simplified compared to the natural communities from which they were derived. Several members of the lignin-adapted and switchgrass-adapted consortia are related to organisms previously characterized as biomass degraders, while others were from less well-characterized phyla. The decrease in complexity of these communities make them good candidates for metagenomic sequencing and will likely enable the reconstruction of a greater number of full-length genes, leading to the discovery of novel lignocellulose-degrading enzymes adapted to feedstocks and conditions of interest.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2010Full-Text: https://escholarship.org/uc/item/5bh2q8rdData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaThe University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)University of North Texas: UNT Digital LibraryArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-010-9089-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen bronze 96 citations 96 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2010Full-Text: https://escholarship.org/uc/item/5bh2q8rdData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaThe University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)University of North Texas: UNT Digital LibraryArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-010-9089-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015 United StatesPublisher:Oxford University Press (OUP) Aifen Zhou; Matthew W. Fields; David A. Stahl; Judy D. Wall; Adam P. Arkin; Grant M. Zane; Terry C. Hazen; Yuanyuan Qu; Yuanyuan Qu; Qichao Tu; Wendy Schackwitz; Jizhong Zhou; Jizhong Zhou; Jizhong Zhou; Zhili He; Qiao Ma; Qiao Ma; Kristina L. Hillesland;Abstract To investigate the genetic basis of microbial evolutionary adaptation to salt (NaCl) stress, populations of Desulfovibrio vulgaris Hildenborough (DvH), a sulfate-reducing bacterium important for the biogeochemical cycling of sulfur, carbon and nitrogen, and potentially the bioremediation of toxic heavy metals and radionuclides, were propagated under salt stress or non-stress conditions for 1200 generations. Whole-genome sequencing revealed 11 mutations in salt stress-evolved clone ES9-11 and 14 mutations in non-stress-evolved clone EC3-10. Whole-population sequencing data suggested the rapid selective sweep of the pre-existing polymorphisms under salt stress within the first 100 generations and the slow fixation of new mutations. Population genotyping data demonstrated that the rapid selective sweep of pre-existing polymorphisms was common in salt stress-evolved populations. In contrast, the selection of pre-existing polymorphisms was largely random in EC populations. Consistently, at 100 generations, stress-evolved population ES9 showed improved salt tolerance, namely increased growth rate (2.0-fold), higher biomass yield (1.8-fold) and shorter lag phase (0.7-fold) under higher salinity conditions. The beneficial nature of several mutations was confirmed by site-directed mutagenesis. All four tested mutations contributed to the shortened lag phases under higher salinity condition. In particular, compared with the salt tolerance improvement in ES9-11, a mutation in a histidine kinase protein gene lytS contributed 27% of the growth rate increase and 23% of the biomass yield increase while a mutation in hypothetical gene DVU2472 contributed 24% of the biomass yield increase. Our results suggested that a few beneficial mutations could lead to dramatic improvements in salt tolerance.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2015Full-Text: https://escholarship.org/uc/item/90g9t37jData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of CaliforniaThe ISME JournalArticle . 2015 . Peer-reviewedLicense: OUP Standard Publication ReuseData sources: CrossrefeScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ismej.2015.45&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen bronze 24 citations 24 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2015Full-Text: https://escholarship.org/uc/item/90g9t37jData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of CaliforniaThe ISME JournalArticle . 2015 . Peer-reviewedLicense: OUP Standard Publication ReuseData sources: CrossrefeScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ismej.2015.45&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2016 United StatesPublisher:Elsevier BV Paradis, Charles J; Jagadamma, Sindhu; Watson, David B; McKay, Larry D; Hazen, Terry C; Park, Melora; Istok, Jonathan D;pmid: 26897652
Reoxidation and mobilization of previously reduced and immobilized uranium by dissolved-phase oxidants poses a significant challenge for remediating uranium-contaminated groundwater. Preferential oxidation of reduced sulfur-bearing species, as opposed to reduced uranium-bearing species, has been demonstrated to limit the mobility of uranium at the laboratory scale yet field-scale investigations are lacking. In this study, the mobility of uranium in the presence of nitrate oxidant was investigated in a shallow groundwater system after establishing conditions conducive to uranium reduction and the formation of reduced sulfur-bearing species. A series of three injections of groundwater (200 L) containing U(VI) (5 μM) and amended with ethanol (40 mM) and sulfate (20 mM) were conducted in ten test wells in order to stimulate microbial-mediated reduction of uranium and the formation of reduced sulfur-bearing species. Simultaneous push-pull tests were then conducted in triplicate well clusters to investigate the mobility of U(VI) under three conditions: 1) high nitrate (120 mM), 2) high nitrate (120 mM) with ethanol (30 mM), and 3) low nitrate (2 mM) with ethanol (30 mM). Dilution-adjusted breakthrough curves of ethanol, nitrate, nitrite, sulfate, and U(VI) suggested that nitrate reduction was predominantly coupled to the oxidation of reduced-sulfur bearing species, as opposed to the reoxidation of U(IV), under all three conditions for the duration of the 36-day tests. The amount of sulfate, but not U(VI), recovered during the push-pull tests was substantially more than injected, relative to bromide tracer, under all three conditions and further suggested that reduced sulfur-bearing species were preferentially oxidized under nitrate-reducing conditions. However, some reoxidation of U(IV) was observed under nitrate-reducing conditions and in the absence of detectable nitrate and/or nitrite. This suggested that reduced sulfur-bearing species may not be fully effective at limiting the mobility of uranium in the presence of dissolved and/or solid-phase oxidants. The results of this field study confirmed those of previous laboratory studies which suggested that reoxidation of uranium under nitrate-reducing conditions can be substantially limited by preferential oxidation of reduced sulfur-bearing species.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2016Full-Text: https://escholarship.org/uc/item/4sm935dpData sources: Bielefeld Academic Search Engine (BASE)Journal of Contaminant HydrologyArticle . 2016 . Peer-reviewedLicense: CC BY NC NDData sources: CrossrefJournal of Contaminant HydrologyArticle . 2016License: CC BY NC NDData sources: BASE (Open Access Aggregator)eScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jconhyd.2016.02.002&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2016Full-Text: https://escholarship.org/uc/item/4sm935dpData sources: Bielefeld Academic Search Engine (BASE)Journal of Contaminant HydrologyArticle . 2016 . Peer-reviewedLicense: CC BY NC NDData sources: CrossrefJournal of Contaminant HydrologyArticle . 2016License: CC BY NC NDData sources: BASE (Open Access Aggregator)eScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jconhyd.2016.02.002&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2011 Australia, United States, United States, United StatesPublisher:Public Library of Science (PLoS) Funded by:NSF | Iron Redox Biogeochemistr..., NSF | LTER: Long-Term Ecologica...NSF| Iron Redox Biogeochemistry: Controls on Carbon and Phosphorus Cycling in Humid Tropical Forests ,NSF| LTER: Long-Term Ecological Research in the Luquillo Experimental Forest 3Phillip Hugenholtz; Phillip Hugenholtz; Yaucin Chavarria; Whendee L. Silver; Whendee L. Silver; Terry C. Hazen; Terry C. Hazen; Kristen M. DeAngelis; Martin Allgaier; Martin Allgaier; Julian L. Fortney; Blake A. Simmons; Blake A. Simmons; Kerry L. Sublette;Lignin is often the most difficult portion of plant biomass to degrade, with fungi generally thought to dominate during late stage decomposition. Lignin in feedstock plant material represents a barrier to more efficient plant biomass conversion and can also hinder enzymatic access to cellulose, which is critical for biofuels production. Tropical rain forest soils in Puerto Rico are characterized by frequent anoxic conditions and fluctuating redox, suggesting the presence of lignin-degrading organisms and mechanisms that are different from known fungal decomposers and oxygen-dependent enzyme activities. We explored microbial lignin-degraders by burying bio-traps containing lignin-amended and unamended biosep beads in the soil for 1, 4, 13 and 30 weeks. At each time point, phenol oxidase and peroxidase enzyme activity was found to be elevated in the lignin-amended versus the unamended beads, while cellulolytic enzyme activities were significantly depressed in lignin-amended beads. Quantitative PCR of bacterial communities showed more bacterial colonization in the lignin-amended compared to the unamended beads after one and four weeks, suggesting that the lignin supported increased bacterial abundance. The microbial community was analyzed by small subunit 16S ribosomal RNA genes using microarray (PhyloChip) and by high-throughput amplicon pyrosequencing based on universal primers targeting bacterial, archaeal, and eukaryotic communities. Community trends were significantly affected by time and the presence of lignin on the beads. Lignin-amended beads have higher relative abundances of representatives from the phyla Actinobacteria, Firmicutes, Acidobacteria and Proteobacteria compared to unamended beads. This study suggests that in low and fluctuating redox soils, bacteria could play a role in anaerobic lignin decomposition.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2011Full-Text: https://escholarship.org/uc/item/65n842cxData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaUniversity of North Texas: UNT Digital LibraryArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1371/journal.pone.0019306&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 179 citations 179 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2011Full-Text: https://escholarship.org/uc/item/65n842cxData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaUniversity of North Texas: UNT Digital LibraryArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1371/journal.pone.0019306&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2020 United StatesPublisher:Elsevier BV Moon, Ji-Won; Paradis, Charles J; Joyner, Dominique C; von Netzer, Frederick; Majumder, Erica L; Dixon, Emma R; Podar, Mircea; Ge, Xiaoxuan; Walian, Peter J; Smith, Heidi J; Wu, Xiaoqin; Zane, Grant M; Walker, Kathleen F; Thorgersen, Michael P; Poole Ii, Farris L; Lui, Lauren M; Adams, Benjamin G; De León, Kara B; Brewer, Sheridan S; Williams, Daniel E; Lowe, Kenneth A; Rodriguez, Miguel; Mehlhorn, Tonia L; Pfiffner, Susan M; Chakraborty, Romy; Arkin, Adam P; Wall, Judy D; Fields, Matthew W; Adams, Michael WW; Stahl, David A; Elias, Dwayne A; Hazen, Terry C;pmid: 32417512
The processing of sediment to accurately characterize the spatially-resolved depth profiles of geophysical and geochemical properties along with signatures of microbial density and activity remains a challenge especially in complex contaminated areas. This study processed cores from two sediment boreholes from background and contaminated core sediments and surrounding groundwater. Fresh core sediments were compared by depth to capture the changes in sediment structure, sediment minerals, biomass, and pore water geochemistry in terms of major and trace elements including pollutants, cations, anions, and organic acids. Soil porewater samples were matched to groundwater level, flow rate, and preferential flows and compared to homogenized groundwater-only samples from neighboring monitoring wells. Groundwater analysis of nearby wells only revealed high sulfate and nitrate concentrations while the same analysis using sediment pore water samples with depth was able to suggest areas high in sulfate- and nitrate-reducing bacteria based on their decreased concentration and production of reduced by-products that could not be seen in the groundwater samples. Positive correlations among porewater content, total organic carbon, trace metals and clay minerals revealed a more complicated relationship among contaminant, sediment texture, groundwater table, and biomass. The fluctuating capillary interface had high concentrations of Fe and Mn-oxides combined with trace elements including U, Th, Sr, Ba, Cu, and Co. This suggests the mobility of potentially hazardous elements, sediment structure, and biogeochemical factors are all linked together to impact microbial communities, emphasizing that solid interfaces play an important role in determining the abundance of bacteria in the sediments.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2020Full-Text: https://escholarship.org/uc/item/8w8633c6Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2020Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2020.126951&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 19 citations 19 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2020Full-Text: https://escholarship.org/uc/item/8w8633c6Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2020Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2020.126951&type=result"></script>'); --> </script>
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description Publicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Elsevier BV Steven Koenigsberg; Mark E. Conrad; Carlos Rios-Velazquez; Ramon E. Martinez; Boris Faybishenko; Josue Malave; Eoin L. Brodie; Terry C. Hazen; Anna Willett; Donald J. Herman; Benjamin V. Mork; Mary K. Firestone; Dominique C. Joyner;pmid: 21872904
To evaluate the efficacy of bioimmobilization of Cr(VI) in groundwater at the Department of Energy Hanford site, we conducted a series of microcosm experiments using a range of commercial electron donors with varying degrees of lactate polymerization (polylactate). These experiments were conducted using Hanford Formation sediments (coarse sand and gravel) immersed in Hanford groundwater, which were amended with Cr(VI) and several types of lactate-based electron donors (Hydrogen Release Compound, HRC; primer-HRC, pHRC; extended release HRC) and the polylactate-cysteine form (Metal Remediation Compound, MRC). The results showed that polylactate compounds stimulated an increase in bacterial biomass and activity to a greater extent than sodium lactate when applied at equivalent carbon concentrations. At the same time, concentrations of headspace hydrogen and methane increased and correlated with changes in the microbial community structure. Enrichment of Pseudomonas spp. occurred with all lactate additions, and enrichment of sulfate-reducing Desulfosporosinus spp. occurred with almost complete sulfate reduction. The results of these experiments demonstrate that amendment with the pHRC and MRC forms result in effective removal of Cr(VI) from solution most likely by both direct (enzymatic) and indirect (microbially generated reductant) mechanisms.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2011.07.021&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu51 citations 51 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2011.07.021&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2017 United StatesPublisher:Wiley Alex J. Lewis; Alex J. Lewis; Maria Fernanda Campa; Terry C. Hazen; Abhijeet P. Borole;SummaryLeveraging nature's biocomplexity for solving human problems requires better understanding of the syntrophic relationships in engineered microbiomes developed in bioreactor systems. Understanding the interactions between microbial players within the community will be key to enhancing conversion and production rates from biomass streams. Here we investigate a bioelectrochemical system employing an enriched microbial consortium for conversion of a switchgrass‐derived bio‐oil aqueous phase (BOAP) into hydrogen via microbial electrolysis (MEC). MECs offer the potential to produce hydrogen in an integrated fashion in biorefinery platforms and as a means of energy storage through decentralized production to supply hydrogen to fuelling stations, as the world strives to move towards cleaner fuels and electricity‐mediated transportation. A unique approach combining differential substrate and redox conditions revealed efficient but rate‐limiting fermentation of the compounds within BOAP by the anode microbial community through a division of labour strategy combined with multiple levels of syntrophy. Despite the fermentation limitation, the adapted abilities of the microbial community resulted in a high hydrogen productivity of 9.35 L per L‐day. Using pure acetic acid as the substrate instead of the biomass‐derived stream resulted in a three‐fold improvement in productivity. This high rate of exoelectrogenesis signifies the potential commercial feasibility of MEC technology for integration in biorefineries.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2018Full-Text: https://escholarship.org/uc/item/9fg7h8z9Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1111/1751-7915.12756&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 26 citations 26 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2018Full-Text: https://escholarship.org/uc/item/9fg7h8z9Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2018Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1111/1751-7915.12756&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2011 AustraliaPublisher:Royal Society of Chemistry (RSC) April Wong; April Wong; Wolfgang Reindl; Gang Cheng; Gang Cheng; Seema Singh; Seema Singh; Terry C. Hazen; C.-H. Yao; Trent R. Northen; Kai Deng; Kai Deng; Anup K. Singh; Anup K. Singh; J.-C. Lee; Steven W. Singer; John M. Gladden; John M. Gladden; Blake A. Simmons; Blake A. Simmons; Paul D. Adams;doi: 10.1039/c1ee01112j
The enzymatic hydrolysis of polysaccharides into fermentable sugars is a crucial step in the conversion of biomass to lignocellulosic biofuels. An efficient hydrolysis is highly dependent on the identification and characterization of optimal glycoside hydrolases. However, existing techniques for characterizing activity are limited by the range of reaction conditions that can be used, sample complexity, and throughput. The method we present is a multiplexed approach based on nanostructure-initiator mass spectrometry (NIMS) that allows for the rapid analysis of several glycolytic activities in parallel under diverse assay conditions. By forming colloids, it was possible to perform aqueous reactions in tubes and microwell plates despite the substrate analogs' hydrophobic perfluorinated tags. This method was validated by analyzing standard enzymatic parameters (temperature, pH, and kinetics) of β-glucosidase and β-xylosidase in separate setups. The multiplexity of this assay system was demonstrated by the simultaneous analysis of β-glucosidase and β-xylosidase activities, which was then used to profile environmental samples. Enzymes secreted by microbial communities within these samples were extracted by washing the sample with buffer. Enzymatic activities were directly detected within this crude extract without any further sample pretreatment steps. The multiplexed analysis of β-glucosidase, exo-/endoglucanase, and xylanase activities was applied for a detailed characterization of previously unknown glycoside hydrolase activities. The results show the suitability of the described method for the rapid screening of crude environmental samples under a wide range of conditions to determine enzyme activities from the microbial communities present within these samples.
Energy & Environment... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/c1ee01112j&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Energy & Environment... arrow_drop_down The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/c1ee01112j&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2008 United StatesPublisher:Wiley Samuel A. Myers; Terry C. Hazen; Harvey W. Blanch; Harvey W. Blanch; Harvey W. Blanch; David S. Reichmuth; Dominique C. Joyner; Rajat Sapra; Rajat Sapra; Yinjie J. Tang; Jay D. Keasling;doi: 10.1002/bit.22181
pmid: 19016470
AbstractA recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C‐based flux analysis to provide insights into the physiological properties of this extremophile and explore its metabolism for bio‐ethanol or other bioprocess applications. Our findings show that glucose metabolism in G. thermoglucosidasius M10EXG proceeds via glycolysis, the pentose phosphate pathway, and the TCA cycle; the Entner–Doudoroff pathway and transhydrogenase activity were not detected. Anaplerotic reactions (including the glyoxylate shunt, pyruvate carboxylase, and phosphoenolpyruvate carboxykinase) were active, but fluxes through those pathways could not be accurately determined using amino acid labeling. When growth conditions were switched from aerobic to micro‐aerobic conditions, fluxes (based on a normalized glucose uptake rate of 100 units (g DCW)−1 h−1) through the TCA cycle and oxidative pentose phosphate pathway were reduced from 64 ± 3 to 25 ± 2 and from 30 ± 2 to 19 ± 2, respectively. The carbon flux under micro‐aerobic growth was directed to ethanol, L‐lactate (>99% optical purity), acetate, and formate. Under fully anerobic conditions, G. thermoglucosidasius M10EXG used a mixed acid fermentation process and exhibited a maximum ethanol yield of 0.38 ± 0.07 mol mol−1 glucose. In silico flux balance modeling demonstrates that lactate and acetate production from G. thermoglucosidasius M10EXG reduces the maximum ethanol yield by approximately threefold, thus indicating that both pathways should be modified to maximize ethanol production. Biotechnol. Bioeng. 2009;102: 1377–1386. © 2008 Wiley Periodicals, Inc.
Biotechnology and Bi... arrow_drop_down Biotechnology and BioengineeringArticle . 2008 . Peer-reviewedLicense: Wiley Online Library User AgreementData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/bit.22181&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 58 citations 58 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Biotechnology and Bi... arrow_drop_down Biotechnology and BioengineeringArticle . 2008 . Peer-reviewedLicense: Wiley Online Library User AgreementData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/bit.22181&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010Publisher:American Society for Microbiology Kathryne G. Byrne-Bailey; Ryan A. Melnyk; Terry C. Hazen; John D. Coates; Peter Agbo; Kelly C. Wrighton;ABSTRACT “ Thermincola potens ” strain JR is one of the first Gram-positive dissimilatory metal-reducing bacteria (DMRB) for which there is a complete genome sequence. Consistent with the physiology of this organism, preliminary annotation revealed an abundance of multiheme c -type cytochromes that are putatively associated with the periplasm and cell surface in a Gram-positive bacterium. Here we report the complete genome sequence of strain JR.
Journal of Bacteriol... arrow_drop_down Journal of BacteriologyArticle . 2010 . Peer-reviewedLicense: ASM Journals Non-Commercial TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1128/jb.00044-10&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 39 citations 39 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert Journal of Bacteriol... arrow_drop_down Journal of BacteriologyArticle . 2010 . Peer-reviewedLicense: ASM Journals Non-Commercial TDMData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1128/jb.00044-10&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2010 Australia, United States, United States, United StatesPublisher:Springer Science and Business Media LLC Whendee L. Silver; Whendee L. Silver; Julian L. Fortney; Julian L. Fortney; Blake A. Simmons; Blake A. Simmons; Jean S. Vander Gheynst; Jean S. Vander Gheynst; Amitha M. Reddy; Amitha M. Reddy; Patrik D'haeseleer; Patrik D'haeseleer; Philip Hugenholtz; Philip Hugenholtz; Kristen M. DeAngelis; Kristen M. DeAngelis; Terry C. Hazen; Terry C. Hazen; Steven W. Singer; Steven W. Singer; John M. Gladden; John M. Gladden; Martin Allgaier; Martin Allgaier;Producing cellulosic biofuels from plant material has recently emerged as a key US Department of Energy goal. For this technology to be commercially viable on a large scale, it is critical to make production cost efficient by streamlining both the deconstruction of lignocellulosic biomass and fuel production. Many natural ecosystems efficiently degrade lignocellulosic biomass and harbor enzymes that, when identified, could be used to increase the efficiency of commercial biomass deconstruction. However, ecosystems most likely to yield relevant enzymes, such as tropical rain forest soil in Puerto Rico, are often too complex for enzyme discovery using current metagenomic sequencing technologies. One potential strategy to overcome this problem is to selectively cultivate the microbial communities from these complex ecosystems on biomass under defined conditions, generating less complex biomass-degrading microbial populations. To test this premise, we cultivated microbes from Puerto Rican soil or green waste compost under precisely defined conditions in the presence dried ground switchgrass (Panicum virgatum L.) or lignin, respectively, as the sole carbon source. Phylogenetic profiling of the two feedstock-adapted communities using SSU rRNA gene amplicon pyrosequencing or phylogenetic microarray analysis revealed that the adapted communities were significantly simplified compared to the natural communities from which they were derived. Several members of the lignin-adapted and switchgrass-adapted consortia are related to organisms previously characterized as biomass degraders, while others were from less well-characterized phyla. The decrease in complexity of these communities make them good candidates for metagenomic sequencing and will likely enable the reconstruction of a greater number of full-length genes, leading to the discovery of novel lignocellulose-degrading enzymes adapted to feedstocks and conditions of interest.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2010Full-Text: https://escholarship.org/uc/item/5bh2q8rdData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaThe University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)University of North Texas: UNT Digital LibraryArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-010-9089-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen bronze 96 citations 96 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2010Full-Text: https://escholarship.org/uc/item/5bh2q8rdData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2010Data sources: eScholarship - University of CaliforniaThe University of Queensland: UQ eSpaceArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)University of North Texas: UNT Digital LibraryArticle . 2010Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-010-9089-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015 United StatesPublisher:Oxford University Press (OUP) Aifen Zhou; Matthew W. Fields; David A. Stahl; Judy D. Wall; Adam P. Arkin; Grant M. Zane; Terry C. Hazen; Yuanyuan Qu; Yuanyuan Qu; Qichao Tu; Wendy Schackwitz; Jizhong Zhou; Jizhong Zhou; Jizhong Zhou; Zhili He; Qiao Ma; Qiao Ma; Kristina L. Hillesland;Abstract To investigate the genetic basis of microbial evolutionary adaptation to salt (NaCl) stress, populations of Desulfovibrio vulgaris Hildenborough (DvH), a sulfate-reducing bacterium important for the biogeochemical cycling of sulfur, carbon and nitrogen, and potentially the bioremediation of toxic heavy metals and radionuclides, were propagated under salt stress or non-stress conditions for 1200 generations. Whole-genome sequencing revealed 11 mutations in salt stress-evolved clone ES9-11 and 14 mutations in non-stress-evolved clone EC3-10. Whole-population sequencing data suggested the rapid selective sweep of the pre-existing polymorphisms under salt stress within the first 100 generations and the slow fixation of new mutations. Population genotyping data demonstrated that the rapid selective sweep of pre-existing polymorphisms was common in salt stress-evolved populations. In contrast, the selection of pre-existing polymorphisms was largely random in EC populations. Consistently, at 100 generations, stress-evolved population ES9 showed improved salt tolerance, namely increased growth rate (2.0-fold), higher biomass yield (1.8-fold) and shorter lag phase (0.7-fold) under higher salinity conditions. The beneficial nature of several mutations was confirmed by site-directed mutagenesis. All four tested mutations contributed to the shortened lag phases under higher salinity condition. In particular, compared with the salt tolerance improvement in ES9-11, a mutation in a histidine kinase protein gene lytS contributed 27% of the growth rate increase and 23% of the biomass yield increase while a mutation in hypothetical gene DVU2472 contributed 24% of the biomass yield increase. Our results suggested that a few beneficial mutations could lead to dramatic improvements in salt tolerance.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2015Full-Text: https://escholarship.org/uc/item/90g9t37jData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of CaliforniaThe ISME JournalArticle . 2015 . Peer-reviewedLicense: OUP Standard Publication ReuseData sources: CrossrefeScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ismej.2015.45&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen bronze 24 citations 24 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2015Full-Text: https://escholarship.org/uc/item/90g9t37jData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of CaliforniaThe ISME JournalArticle . 2015 . Peer-reviewedLicense: OUP Standard Publication ReuseData sources: CrossrefeScholarship - University of CaliforniaArticle . 2015Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ismej.2015.45&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2016 United StatesPublisher:Elsevier BV Paradis, Charles J; Jagadamma, Sindhu; Watson, David B; McKay, Larry D; Hazen, Terry C; Park, Melora; Istok, Jonathan D;pmid: 26897652
Reoxidation and mobilization of previously reduced and immobilized uranium by dissolved-phase oxidants poses a significant challenge for remediating uranium-contaminated groundwater. Preferential oxidation of reduced sulfur-bearing species, as opposed to reduced uranium-bearing species, has been demonstrated to limit the mobility of uranium at the laboratory scale yet field-scale investigations are lacking. In this study, the mobility of uranium in the presence of nitrate oxidant was investigated in a shallow groundwater system after establishing conditions conducive to uranium reduction and the formation of reduced sulfur-bearing species. A series of three injections of groundwater (200 L) containing U(VI) (5 μM) and amended with ethanol (40 mM) and sulfate (20 mM) were conducted in ten test wells in order to stimulate microbial-mediated reduction of uranium and the formation of reduced sulfur-bearing species. Simultaneous push-pull tests were then conducted in triplicate well clusters to investigate the mobility of U(VI) under three conditions: 1) high nitrate (120 mM), 2) high nitrate (120 mM) with ethanol (30 mM), and 3) low nitrate (2 mM) with ethanol (30 mM). Dilution-adjusted breakthrough curves of ethanol, nitrate, nitrite, sulfate, and U(VI) suggested that nitrate reduction was predominantly coupled to the oxidation of reduced-sulfur bearing species, as opposed to the reoxidation of U(IV), under all three conditions for the duration of the 36-day tests. The amount of sulfate, but not U(VI), recovered during the push-pull tests was substantially more than injected, relative to bromide tracer, under all three conditions and further suggested that reduced sulfur-bearing species were preferentially oxidized under nitrate-reducing conditions. However, some reoxidation of U(IV) was observed under nitrate-reducing conditions and in the absence of detectable nitrate and/or nitrite. This suggested that reduced sulfur-bearing species may not be fully effective at limiting the mobility of uranium in the presence of dissolved and/or solid-phase oxidants. The results of this field study confirmed those of previous laboratory studies which suggested that reoxidation of uranium under nitrate-reducing conditions can be substantially limited by preferential oxidation of reduced sulfur-bearing species.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2016Full-Text: https://escholarship.org/uc/item/4sm935dpData sources: Bielefeld Academic Search Engine (BASE)Journal of Contaminant HydrologyArticle . 2016 . Peer-reviewedLicense: CC BY NC NDData sources: CrossrefJournal of Contaminant HydrologyArticle . 2016License: CC BY NC NDData sources: BASE (Open Access Aggregator)eScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jconhyd.2016.02.002&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 28 citations 28 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2016Full-Text: https://escholarship.org/uc/item/4sm935dpData sources: Bielefeld Academic Search Engine (BASE)Journal of Contaminant HydrologyArticle . 2016 . Peer-reviewedLicense: CC BY NC NDData sources: CrossrefJournal of Contaminant HydrologyArticle . 2016License: CC BY NC NDData sources: BASE (Open Access Aggregator)eScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2016Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.jconhyd.2016.02.002&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2011 Australia, United States, United States, United StatesPublisher:Public Library of Science (PLoS) Funded by:NSF | Iron Redox Biogeochemistr..., NSF | LTER: Long-Term Ecologica...NSF| Iron Redox Biogeochemistry: Controls on Carbon and Phosphorus Cycling in Humid Tropical Forests ,NSF| LTER: Long-Term Ecological Research in the Luquillo Experimental Forest 3Phillip Hugenholtz; Phillip Hugenholtz; Yaucin Chavarria; Whendee L. Silver; Whendee L. Silver; Terry C. Hazen; Terry C. Hazen; Kristen M. DeAngelis; Martin Allgaier; Martin Allgaier; Julian L. Fortney; Blake A. Simmons; Blake A. Simmons; Kerry L. Sublette;Lignin is often the most difficult portion of plant biomass to degrade, with fungi generally thought to dominate during late stage decomposition. Lignin in feedstock plant material represents a barrier to more efficient plant biomass conversion and can also hinder enzymatic access to cellulose, which is critical for biofuels production. Tropical rain forest soils in Puerto Rico are characterized by frequent anoxic conditions and fluctuating redox, suggesting the presence of lignin-degrading organisms and mechanisms that are different from known fungal decomposers and oxygen-dependent enzyme activities. We explored microbial lignin-degraders by burying bio-traps containing lignin-amended and unamended biosep beads in the soil for 1, 4, 13 and 30 weeks. At each time point, phenol oxidase and peroxidase enzyme activity was found to be elevated in the lignin-amended versus the unamended beads, while cellulolytic enzyme activities were significantly depressed in lignin-amended beads. Quantitative PCR of bacterial communities showed more bacterial colonization in the lignin-amended compared to the unamended beads after one and four weeks, suggesting that the lignin supported increased bacterial abundance. The microbial community was analyzed by small subunit 16S ribosomal RNA genes using microarray (PhyloChip) and by high-throughput amplicon pyrosequencing based on universal primers targeting bacterial, archaeal, and eukaryotic communities. Community trends were significantly affected by time and the presence of lignin on the beads. Lignin-amended beads have higher relative abundances of representatives from the phyla Actinobacteria, Firmicutes, Acidobacteria and Proteobacteria compared to unamended beads. This study suggests that in low and fluctuating redox soils, bacteria could play a role in anaerobic lignin decomposition.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2011Full-Text: https://escholarship.org/uc/item/65n842cxData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaUniversity of North Texas: UNT Digital LibraryArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1371/journal.pone.0019306&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 179 citations 179 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2011Full-Text: https://escholarship.org/uc/item/65n842cxData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaeScholarship - University of CaliforniaArticle . 2011Data sources: eScholarship - University of CaliforniaUniversity of North Texas: UNT Digital LibraryArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)The University of Queensland: UQ eSpaceArticle . 2011Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1371/journal.pone.0019306&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2020 United StatesPublisher:Elsevier BV Moon, Ji-Won; Paradis, Charles J; Joyner, Dominique C; von Netzer, Frederick; Majumder, Erica L; Dixon, Emma R; Podar, Mircea; Ge, Xiaoxuan; Walian, Peter J; Smith, Heidi J; Wu, Xiaoqin; Zane, Grant M; Walker, Kathleen F; Thorgersen, Michael P; Poole Ii, Farris L; Lui, Lauren M; Adams, Benjamin G; De León, Kara B; Brewer, Sheridan S; Williams, Daniel E; Lowe, Kenneth A; Rodriguez, Miguel; Mehlhorn, Tonia L; Pfiffner, Susan M; Chakraborty, Romy; Arkin, Adam P; Wall, Judy D; Fields, Matthew W; Adams, Michael WW; Stahl, David A; Elias, Dwayne A; Hazen, Terry C;pmid: 32417512
The processing of sediment to accurately characterize the spatially-resolved depth profiles of geophysical and geochemical properties along with signatures of microbial density and activity remains a challenge especially in complex contaminated areas. This study processed cores from two sediment boreholes from background and contaminated core sediments and surrounding groundwater. Fresh core sediments were compared by depth to capture the changes in sediment structure, sediment minerals, biomass, and pore water geochemistry in terms of major and trace elements including pollutants, cations, anions, and organic acids. Soil porewater samples were matched to groundwater level, flow rate, and preferential flows and compared to homogenized groundwater-only samples from neighboring monitoring wells. Groundwater analysis of nearby wells only revealed high sulfate and nitrate concentrations while the same analysis using sediment pore water samples with depth was able to suggest areas high in sulfate- and nitrate-reducing bacteria based on their decreased concentration and production of reduced by-products that could not be seen in the groundwater samples. Positive correlations among porewater content, total organic carbon, trace metals and clay minerals revealed a more complicated relationship among contaminant, sediment texture, groundwater table, and biomass. The fluctuating capillary interface had high concentrations of Fe and Mn-oxides combined with trace elements including U, Th, Sr, Ba, Cu, and Co. This suggests the mobility of potentially hazardous elements, sediment structure, and biogeochemical factors are all linked together to impact microbial communities, emphasizing that solid interfaces play an important role in determining the abundance of bacteria in the sediments.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2020Full-Text: https://escholarship.org/uc/item/8w8633c6Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2020Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2020.126951&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 19 citations 19 popularity Top 10% influence Average impulse Top 10% Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2020Full-Text: https://escholarship.org/uc/item/8w8633c6Data sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2020Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1016/j.chemosphere.2020.126951&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu