• Energy Research

The contamination of agricultural land with heavy metals is a global concern. Agricultural products produced in heavy metal-contaminated soil are prone to metal accumulation, and thus, are less fitted for consumption due to food safety issues. The cultivation of biofuel crops in contaminated soil would provide immediate economic benefit to the landholders while simultaneously reclaiming contaminated sites in the long run. The use of edible soybean for biodiesel production is discouraged due to the negative impact on food security. However, soybean produced in metal-contaminated soil would be suitable for biodiesel production. In this study, the tolerance and metal bioaccumulation potential of Pseudomonas putida KNP9 for Pb and Cd is investigated, and KNP9 is tested for soybean growth enhancement in cadmium and lead-amended soil. The maximum metal tolerance for the Pb and Cd in KNP9 was 1580 µM and 546 µM, respectively. KNP9 was found to be effective in removing both Pb and Cd from the solution. SEM-EDX revealed that KNP9 bioaccumulates both Pb and Cd. In pot trial studies, KNP9 was found to be effective in enhancing soybean growth with respect to untreated control under lead and cadmium stress. Thus, KNP9 inoculation protects soybean plants from the detrimental effects of cadmium and lead stress. Therefore, metal bioaccumulating bacterium P. putida KNP9 inoculation in soybean is a promising strategy for soybean growth enhancement, which could be utilized for enhanced biodiesel production from soybean at metal-contaminated sites.

The contamination of agricultural land with heavy metals is a global concern. Agricultural products produced in heavy metal-contaminated soil are prone to metal accumulation, and thus, are less fitted for consumption due to food safety issues. The cultivation of biofuel crops in contaminated soil would provide immediate economic benefit to the landholders while simultaneously reclaiming contaminated sites in the long run. The use of edible soybean for biodiesel production is discouraged due to the negative impact on food security. However, soybean produced in metal-contaminated soil would be suitable for biodiesel production. In this study, the tolerance and metal bioaccumulation potential of Pseudomonas putida KNP9 for Pb and Cd is investigated, and KNP9 is tested for soybean growth enhancement in cadmium and lead-amended soil. The maximum metal tolerance for the Pb and Cd in KNP9 was 1580 µM and 546 µM, respectively. KNP9 was found to be effective in removing both Pb and Cd from the solution. SEM-EDX revealed that KNP9 bioaccumulates both Pb and Cd. In pot trial studies, KNP9 was found to be effective in enhancing soybean growth with respect to untreated control under lead and cadmium stress. Thus, KNP9 inoculation protects soybean plants from the detrimental effects of cadmium and lead stress. Therefore, metal bioaccumulating bacterium P. putida KNP9 inoculation in soybean is a promising strategy for soybean growth enhancement, which could be utilized for enhanced biodiesel production from soybean at metal-contaminated sites.

Inappropriate agricultural practices consume more input energy and emit higher greenhouse gases (GHGs) which cause global warming and climate change, thereby threatening environmental sustainability. To identify energy and carbon-efficient varieties and nutrient management practices, the present study was undertaken during the kharif season of 2018 and 2019 in a split-plot design with three varieties of fodder maize (African Tall, J-1006 and P-3396) and four nutrient management practices such as N0: Absolute control, N1: 100% recommended dose of fertilizers (RDF), N2: 75% RDF + plant growth promoting rhizobacteria (PGPR) + Panchagavya spray and N3: 50% RDF + 25% farmyard manure (FYM) + PGPR + Panchagavya spray). Results indicated that variety J-1006 and applying 75% RDF + PGPR + Panchagavya spray produced significantly higher dry fodder yield. Among the varieties, J-1006 recorded the highest total energy output (224,123 MJ ha−1), net energy (211,280 MJ ha−1), energy use efficiency (17.64), energy productivity (0.98 kg MJ−1), energy profitability (16.64), and lowest specific energy (1.03 MJ ha−1). Regarding nutrient management, 75% RDF + PGPR + Panchagavya spray fetched the highest total energy output (229,470 MJ ha−1) and net energy (215,482 MJ ha−1). However, energy use efficiency, energy productivity, and energy profitability were significantly higher with integrated nutrient management (N2 and N3) over 100% RDF. Concerning the carbon estimation, J-1006 resulted in a significantly higher carbon output (5479 kg CE ha−1), net carbon gain (5029 kg CE ha−1), carbon efficiency (12.46), carbon sustainability index (11.46), and significantly lower carbon footprint per unit yield (CFy) (131.3 kg CO2-e Mg−1). For nutrient management, the application of 75% RDF + PGPR + Panchagavya spray showed significantly higher carbon output (5609 kg CE ha−1) and net carbon gain (5112 kg CE ha−1). However, significantly higher carbon efficiency, carbon sustainability index, and lower CFy were reported with integrated nutrient management over 100% RDF. Overall, selecting the J-1006 variety and applying 75% RDF + PGPR + Panchagavya spray for fodder maize cultivation could be the most productive in terms of dry fodder production, energy, and carbon efficiency approach.

Inappropriate agricultural practices consume more input energy and emit higher greenhouse gases (GHGs) which cause global warming and climate change, thereby threatening environmental sustainability. To identify energy and carbon-efficient varieties and nutrient management practices, the present study was undertaken during the kharif season of 2018 and 2019 in a split-plot design with three varieties of fodder maize (African Tall, J-1006 and P-3396) and four nutrient management practices such as N0: Absolute control, N1: 100% recommended dose of fertilizers (RDF), N2: 75% RDF + plant growth promoting rhizobacteria (PGPR) + Panchagavya spray and N3: 50% RDF + 25% farmyard manure (FYM) + PGPR + Panchagavya spray). Results indicated that variety J-1006 and applying 75% RDF + PGPR + Panchagavya spray produced significantly higher dry fodder yield. Among the varieties, J-1006 recorded the highest total energy output (224,123 MJ ha−1), net energy (211,280 MJ ha−1), energy use efficiency (17.64), energy productivity (0.98 kg MJ−1), energy profitability (16.64), and lowest specific energy (1.03 MJ ha−1). Regarding nutrient management, 75% RDF + PGPR + Panchagavya spray fetched the highest total energy output (229,470 MJ ha−1) and net energy (215,482 MJ ha−1). However, energy use efficiency, energy productivity, and energy profitability were significantly higher with integrated nutrient management (N2 and N3) over 100% RDF. Concerning the carbon estimation, J-1006 resulted in a significantly higher carbon output (5479 kg CE ha−1), net carbon gain (5029 kg CE ha−1), carbon efficiency (12.46), carbon sustainability index (11.46), and significantly lower carbon footprint per unit yield (CFy) (131.3 kg CO2-e Mg−1). For nutrient management, the application of 75% RDF + PGPR + Panchagavya spray showed significantly higher carbon output (5609 kg CE ha−1) and net carbon gain (5112 kg CE ha−1). However, significantly higher carbon efficiency, carbon sustainability index, and lower CFy were reported with integrated nutrient management over 100% RDF. Overall, selecting the J-1006 variety and applying 75% RDF + PGPR + Panchagavya spray for fodder maize cultivation could be the most productive in terms of dry fodder production, energy, and carbon efficiency approach.