• Energy Research

Short-rotation coppice (SRC) Salix plantations have the potential to provide fast-growing biomass feedstock with significant soil and climate mitigation benefits. Salix varieties exhibit significant variation in their physiological traits, growth patterns and soil ecology—but the effects of these variations have rarely been studied from a systems perspective. This study analyses the influence of variety on soil organic carbon (SOC) dynamics and climate impacts from Salix cultivation for heat production for a Swedish site with specific conditions. Soil carbon modelling was combined with a life cycle assessment (LCA) approach to quantify SOC sequestration and climate impacts over a 50-year period. The analysis used data from a Swedish field trial of six Salix varieties grown under fertilized and unfertilized treatments on Vertic Cambisols during 2001–2018. The Salix systems were compared with a reference case where heat is produced from natural gas and green fallow was the land use alternative. Climate impacts were determined using time-dependent LCA methodology—on a land-use (per hectare) and delivered energy unit (per MJheat) basis. All Salix varieties and treatments increased SOC, but the magnitude depended on the variety. Fertilization led to lower carbon sequestration than the equivalent unfertilized case. There was no clear relationship between biomass yield and SOC increase. In comparison with reference cases, all Salix varieties had significant potential for climate change mitigation. From a land-use perspective, high yield was the most important determining factor, followed by SOC sequestration, therefore high-yielding fertilized varieties such as ‘Tordis’, ‘Tora’ and ‘Björn’ performed best. On an energy-delivered basis, SOC sequestration potential was the determining factor for the climate change mitigation effect, with unfertilized ‘Jorr’ and ‘Loden’ outperforming the other varieties. These results show that Salix variety has a strong influence on SOC sequestration potential, biomass yield, growth pattern, response to fertilization and, ultimately, climate impact.

Short-rotation coppice (SRC) Salix plantations have the potential to provide fast-growing biomass feedstock with significant soil and climate mitigation benefits. Salix varieties exhibit significant variation in their physiological traits, growth patterns and soil ecology—but the effects of these variations have rarely been studied from a systems perspective. This study analyses the influence of variety on soil organic carbon (SOC) dynamics and climate impacts from Salix cultivation for heat production for a Swedish site with specific conditions. Soil carbon modelling was combined with a life cycle assessment (LCA) approach to quantify SOC sequestration and climate impacts over a 50-year period. The analysis used data from a Swedish field trial of six Salix varieties grown under fertilized and unfertilized treatments on Vertic Cambisols during 2001–2018. The Salix systems were compared with a reference case where heat is produced from natural gas and green fallow was the land use alternative. Climate impacts were determined using time-dependent LCA methodology—on a land-use (per hectare) and delivered energy unit (per MJheat) basis. All Salix varieties and treatments increased SOC, but the magnitude depended on the variety. Fertilization led to lower carbon sequestration than the equivalent unfertilized case. There was no clear relationship between biomass yield and SOC increase. In comparison with reference cases, all Salix varieties had significant potential for climate change mitigation. From a land-use perspective, high yield was the most important determining factor, followed by SOC sequestration, therefore high-yielding fertilized varieties such as ‘Tordis’, ‘Tora’ and ‘Björn’ performed best. On an energy-delivered basis, SOC sequestration potential was the determining factor for the climate change mitigation effect, with unfertilized ‘Jorr’ and ‘Loden’ outperforming the other varieties. These results show that Salix variety has a strong influence on SOC sequestration potential, biomass yield, growth pattern, response to fertilization and, ultimately, climate impact.

Short rotation coppice (SRC) systems can play a role as feedstock for bioenergy supply contributing to EU energy and climate policy targets. A scenario depicting intensive arable crop cultivation in a homogeneous landscape (lacking habitat structures) was compared to a scenario including SRC cultivation on 20 % of arable land. A range of indicators was selected to assess the consequences of SRC on soil, water and biodiversity, using data from the Rating-SRC project (Sweden and Germany). The results of the assessment were presented using spider diagrams. Establishment and use of SRC for bioenergy has both positive and negative effects. The former include increased carbon sequestration and reduced GHG emissions as well as reduced soil erosion, groundwater nitrate and surface runoff. SRC can be used in phytoremediation and improves plant and breeding bird biodiversity (exceptions: grassland and arable land species) but should not be applied in dry areas or on soils high in toxic trace elements (exception: cadmium). The scenario-based analysis was found useful for studying the consequences of SRC cultivation at larger scales. Limitations of the approach are related to data requirements and compatibility and its restricted ability to cover spatial diversity and dynamic processes. The findings should not be generalised beyond the representativeness of the data used.

Short rotation coppice (SRC) systems can play a role as feedstock for bioenergy supply contributing to EU energy and climate policy targets. A scenario depicting intensive arable crop cultivation in a homogeneous landscape (lacking habitat structures) was compared to a scenario including SRC cultivation on 20 % of arable land. A range of indicators was selected to assess the consequences of SRC on soil, water and biodiversity, using data from the Rating-SRC project (Sweden and Germany). The results of the assessment were presented using spider diagrams. Establishment and use of SRC for bioenergy has both positive and negative effects. The former include increased carbon sequestration and reduced GHG emissions as well as reduced soil erosion, groundwater nitrate and surface runoff. SRC can be used in phytoremediation and improves plant and breeding bird biodiversity (exceptions: grassland and arable land species) but should not be applied in dry areas or on soils high in toxic trace elements (exception: cadmium). The scenario-based analysis was found useful for studying the consequences of SRC cultivation at larger scales. Limitations of the approach are related to data requirements and compatibility and its restricted ability to cover spatial diversity and dynamic processes. The findings should not be generalised beyond the representativeness of the data used.

Fast-growing willows (Salix spp.) provide alternative sources of renewable energy generation, but need an adequate nutrient availability in the soil for high biomass production. In general, species mixtures can be more nutrient-efficient than pure cultures, but this is scarcely known for Salix spp. Therefore, this study evaluates the nutrient availability and P mobilization under two willow species, Salixdasyclados var. ‘Loden’ and S. schwerinii × viminalis var. ‘Tora’, grown as pure and mixed cultures at non-fertilized former arable sites in Germany (Stagnic Cambisol) and Sweden (Vertic Cambisol). The plant availability of potassium (K), magnesium (Mg) and phosphorus (P) and soil phosphatase activities in the topsoil were measured in spring of the year of planting (initial) and under 4 years-old stocks (one year after the first 3-year cutting cycle). The initial plant availability of the nutrients significantly differed between the sites and the two sampling dates at both sites. The plant availability of K and Mg was optimal to high at both sites and sampling dates, but rather low for P (after 4 years ≤5 mg P 100 g−1 soil). The plant-available P and K content in soil significantly decreased within the 4 years of willow growth at both sites. The acid and alkaline phosphatase activity in the soil of the German site (Rostock) was significantly lower after 4 years of willow growth, but differed not significantly between the two sampling dates at the Swedish site (Uppsala). Higher activity of acid phosphatase compared to alkaline phosphatase was recorded in the soils at both test sites based on the site-specific soil pH (<7). The slight decrease of plant availability of P after 4 years of Salix growth in pure culture differed not significantly between the different species. Mixed growth did not decrease the plant availability of P within this period, although no significant difference in the biomass production of pure and mixed growth was observed. This was valid at both sites, and therefore, seems independent of the site-specific differences in soil and climate conditions. The general validity of the assumptions should be tested also for other species mixtures and soil conditions in the future before site-adapted growth designs can be recommended in biomass production of Salix.

Fast-growing willows (Salix spp.) provide alternative sources of renewable energy generation, but need an adequate nutrient availability in the soil for high biomass production. In general, species mixtures can be more nutrient-efficient than pure cultures, but this is scarcely known for Salix spp. Therefore, this study evaluates the nutrient availability and P mobilization under two willow species, Salixdasyclados var. ‘Loden’ and S. schwerinii × viminalis var. ‘Tora’, grown as pure and mixed cultures at non-fertilized former arable sites in Germany (Stagnic Cambisol) and Sweden (Vertic Cambisol). The plant availability of potassium (K), magnesium (Mg) and phosphorus (P) and soil phosphatase activities in the topsoil were measured in spring of the year of planting (initial) and under 4 years-old stocks (one year after the first 3-year cutting cycle). The initial plant availability of the nutrients significantly differed between the sites and the two sampling dates at both sites. The plant availability of K and Mg was optimal to high at both sites and sampling dates, but rather low for P (after 4 years ≤5 mg P 100 g−1 soil). The plant-available P and K content in soil significantly decreased within the 4 years of willow growth at both sites. The acid and alkaline phosphatase activity in the soil of the German site (Rostock) was significantly lower after 4 years of willow growth, but differed not significantly between the two sampling dates at the Swedish site (Uppsala). Higher activity of acid phosphatase compared to alkaline phosphatase was recorded in the soils at both test sites based on the site-specific soil pH (<7). The slight decrease of plant availability of P after 4 years of Salix growth in pure culture differed not significantly between the different species. Mixed growth did not decrease the plant availability of P within this period, although no significant difference in the biomass production of pure and mixed growth was observed. This was valid at both sites, and therefore, seems independent of the site-specific differences in soil and climate conditions. The general validity of the assumptions should be tested also for other species mixtures and soil conditions in the future before site-adapted growth designs can be recommended in biomass production of Salix.

We introduce an easy, fast and effective method to analyze the influence of genetically modified (GM) plants on soil and model organisms in the laboratory to substitute laborious and time consuming field trials. For the studies described here we focused on two GM plants of the so-called 3rd generation: GM plants producing pharmaceuticals (PMP) and plant made industrials (PMI). Cyanophycin synthetase (cphA) was chosen as model for PMI and Choleratoxin B (CTB) as model for PMP. The model genes are expressed in transgenic roots of composite Vicia hirsuta plants grown in petri dishes for semi-sterile growth or small containers filled with non-sterile soil. No significant influence of the model gene expression on root induction, growth, biomass, interaction with symbionts such as rhizobia (number, size and functionality of nodules, selection of nodulating strains) or arbuscular mycorrhizal fungi could be detected. In vitro, but not in situ under field conditions, structural diversity of the bulk soil microbial community between transgenic and non-transgenic cultivars was determined by PLFA pattern-derived ratios of bacteria: fungi and of gram+: gram- bacteria. Significant differences in PLFA ratios were associated with dissimilarities in the quantity and molecular composition of rhizodeposits as revealed by Py-FIMS analyses. Contrary to field trials, where small effects based on the transgene expression might be hidden by the immense influence of various environmental factors, our in vitro system can detect even minor effects and correlates them to transgene expression with less space, time and labour.