• Energy Research

In response to the growing threat to the quality of the soil environment, new technologies are being developed to protect and remediate contaminated sites. A new approach, namely, assisted phytostabilization, has been used in areas contaminated with high levels of potentially toxic elements (PTEs), using various soil additives. This paper determined the effectiveness of biochar-assisted phytostabilization using Dactylis glomerata L. of soil contaminated with high concentrations of the selected PTEs (in mg/kg soil): Cu (780 ± 144), Cd (25.9 ± 2.5), Pb (13,540 ± 669) and Zn (8433 ± 1376). The content of the selected PTEs in the roots and above-ground parts of the tested grass, and in the soil, was determined by atomic absorption spectrometry (AAS). The addition of biochar to the contaminated soil led to an increase in plant biomass and caused an increase in soil pH values. Concentrations of Cu, Cd, Pb and Zn were higher in the roots than in the above-ground parts of Dactylis glomerata L. The application of biochar significantly reduced the total content of PTEs in the soil after finishing the phytostabilization experiment, as well as reducing the content of bioavailable forms extracted from the soil using CaCl2 solution, which was clearly visible with respect to Cd and Pb. It is concluded that the use of biochar in supporting the processes of assisted phytostabilization of soils contaminated with PTEs is justified.

Poly-3-hydroxybutyrate (P3HB), a biopolymer synthesized by soil bacteria, has emerged as a promising tool for sustainable agriculture, offering dual benefits as a carbon reservoir and an eco-friendly biotechnological product. However, its impact on soil nutrient dynamics and plant nutrient uptake remains underexplored. This study evaluated the effects of P3HB biodegradation on soil properties and maize (Zea mays) growth in a pot experiment with five P3HB application rates (0-10 % w/w), in both planted and unplanted soils. Key analyses included soil pH, enzyme activity, microbial biomass carbon (MBC), nutrient contents in soil and plant biomass, and residual P3HB (a rarely addressed aspect in previous research). The addition of P3HB influenced soil biota in both planted and unplanted soils, showing consistent trends across application rates. P3HB reduced soil pH (from 7.4 to 7.1 at 1 % and 6.4 at 10 % P3HB in unplanted soil) and increased total carbon (by approximately 100 % in unplanted and 65 % in planted soils at 10 % P3HB). In unplanted soils, P3HB degraded more quickly, but enzyme activities of β-glucosidase and phosphatase decreased by 20 % and 15 %, respectively. Conversely, arylsulphatase and urease activities increased by 80 % and 200 %, respectively, in both soil variants in both variants. Microbial biomass carbon increased by 500 % in unplanted soils compared to the unamended control, while planted soils showed a 10 % increase. Available nutrients (K and P) were higher in unplanted soils compared to planted soils. In planted soils, competition for nutrients (N, P, K) among maize plants, the rhizobiome, and P3HB-degrading microbes led to reduced above-ground biomass at higher P3HB application rates (from 5.6 g to 0.5 g per plant at 1 % P3HB). Statistical analysis (Eta-squared values and ANOVA) revealed that P3HB dose primarily influenced soil physico-chemical properties and plant parameters, whereas maize planting had a smaller impact, affecting only pH and MBC. P3HB biodegradation improved soil properties, particularly by increasing MBC and total carbon. However, application rates of 1 % and above caused slight acidification, increased nutrient competition, and reduced nutrient availability, ultimately hindering maize growth. These results underscore the trade-offs between improving soil quality and maintaining crop productivity, highlighting the importance of optimizing P3HB application rates in agricultural systems. This study provides critical insights into the dual effects of biodegradable plastics like P3HB, emphasizing their potential as microbial carbon storage polymers while cautioning against excessive use in crop production.

AbstractThe overuse of synthetic fertilizers has been associated with negative environmental consequences. The use of biochar in this regard has been recommended as a win–win strategy. However, our understanding on the comparative influences of biochar prepared from various feedstocks mixed with other bulking agents on soil health and crop performance remained limited. Therefore, in the present study, three types of biochar produced from sewage sludge, food, and agricultural waste were analyzed and compared for their effects on soil enzymes (dehydrogenase, DHA; β-glucosidase, GLU; phosphatase, PHOS; urease, URE; N-acetyl-β-D-glucosaminidase, NAG; and arylsulphatase, ARS), soil basal, as well as substrate-induced respirations and plant growth and physiology characters. The results revealed that food waste-derived biochar co-pyrolyzed with zeolite and/or sawdust was more effective in improving soil physicochemical properties and carbon and phosphorous cycling enzyme (DHA, GLU, and PHOS) activities in addition to soil basal respiration. While the influence of wastewater sewage sludge-derived biochar was more pronounced on urease, N-acetyl-β-D-glucosaminidase, and arylsulphatase enzymes as well as plant biomass accumulation and physiological attributes. Moreover, agricultural waste-derived biochar was found to be effective in enhancing substrate-induced respirations. This study thus concluded that biochar derived from various feedstocks has the tendency to improve soil health and plant growth attributes which further depend on the type of modification prior to pyrolysis.

The presence of potentially toxic elements (PTEs) in soils can upset the natural balance and increase the risk of PTE incorporation into the food chain. The use of composite biochar with municipal sewage sludge compost (MSSC/C) can be an effective way of both managing waste, such as sewage sludge, and providing an effective additive-supporting phytostabilization processes. The effectiveness of D. glomerata and MSSC/C in the technique of assisted phytostabilization of industrially contaminated soils was determined under the pot experiment conditions. The PTE contents in D. glomerata and the soil were determined using the spectrophotometric method. The addition of MSSC/C to PTE-contaminated soil contributed to an 18% increase in plant biomass and increased the soil pH by 1.67 units, with the PTE concentration being higher in the roots than in the above-ground parts of D. glomerata. The MSSC/C addition had the strongest effect on the reduction in Cd, Cr, and Ni contents in the soil following the completion of the experiment. The current study confirmed the effectiveness of MSSC/C in aiding the phytostabilization processes in PTE-contaminated soils.

Biochar application to the soil has been recommended as a carbon (C) management approach to sequester C and improve soil quality. Three-year experiments were conducted to investigate the interactive effects of three types of amendments on microbial biomass carbon, soil dehydrogenase activity and soil microbial community abundance in luvisols of arable land in the Czech Republic. Four different treatments were studied, which were, only NPK as a control, NPK + cattle manure, NPK + biochar and NPK + combination of manure with biochar. The results demonstrate that all amendments were effective in increasing the fungal and bacterial biomass, as is evident from the increased values of bacterial and fungal phospholipid fatty acid analysis. The ammonia-oxidizing bacteria population increases with the application of biochar, and it reaches its maximum value when biochar is applied in combination with manure. The overall results suggest that co-application of biochar with manure changes soil properties in favor of increased microbial biomass. It was confirmed that the application of biochar might increase or decrease soil activity, but its addition, along with manure, always promotes microbial abundance and their activity. The obtained results can be used in the planning and execution of the biochar-based soil amendments.

AbstractFarmyard manure is the most common type of organic fertilizer, and its properties depend mainly on the type of livestock, bedding material and the conditions of fermentation. Co-maturing of manure with other amendments to modify its final properties has been seen as a win–win strategy recently. This study aimed to evaluate the differences in the effect of unenriched manure and manures co-matured with biochar, elemental sulfur or both amendments on the soil physico-chemical and biological properties, and plant (barley, maize) biomass production. For this purpose a pot experiment was carried out in a time-dependent way. Samples were taken from 12 week-lasting (test crop barley) and 24 week-lasting (test crop maize) pot cultivation carried out in a growth chamber. Co-matured manure with biochar showed the highest rate of maturation expressed as humic to fulvic acid ratio, its amendment to soil significantly increased the dry aboveground biomass weight in the half-time (12 weeks) of experiment. However, the effect vanished after 24 weeks. We received for this variant highest long-term (24 weeks) contents of total carbon and nitrogen in soil. Contrarily, co-matured manure with biochar and elemental sulfur led to short-term carbon sequestration (the highest total carbon in 12 weeks) due to presumed retardation of microbial-mediated transformation of nutrients. We conclude that the prolonged pot experiment with biochar or elemental sulfur enriched manure led to the increased recalcitrancy of soil organic matter and retardation of soil nutrient transformation to the plant-available form.

Objectives As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. Methods Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids—were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. Results Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. Conclusions The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

(1) Background: sewage sludge is a by-product of wastewater treatment, which needs to be managed appropriately, e.g., in composting processes. The application of municipal sewage sludge composts (MSSCs) as a soil amendment is a potential way to effectively manage sewage sludge. (2) Methods: this paper presents the results of a vegetation pot experiment undertaken to assess the suitability of Dactylis glomerata L. and MSSC in the aided phytostabilization technique when applied on soils from an area effected by industrial pressure; this is characterized by high levels of heavy metal (HM). The contents of HMs in the test plant (the roots and above-ground parts), as well as in the soil and MSSC, were determined via an atomic spectrometry method. (3) Results: the application of MSSC positively contributed to an increased production of plant biomass and an increase in the pH in the soil. Concentrations of Cu, Cd, Pb, Zn, and Cr were higher in the roots than in the above-ground parts of Dactylis glomerata L. The addition of MSSC contributed most significantly to the considerable reduction in Ni, Pb, and Zn contents in the soil after the experiment. (4) Conclusions: MSSC can support the phytostabilization of soils contaminated with high levels of HMs.