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Digital Annex for the following thesis: Vila-Viçosa, C. (2023). Natural History, Biogeography and Evolution of the Iberian white oak syngameon (Quercus L. Sect. Quercus). Ph.D. Thesis, Faculdade de Ciências da Universidade do Porto, Portugal Abstract: The genus Quercus L. is one of the most diverse and important group of woody plants, particularly when considering that they are the trees that rule the Northern Hemisphere forests. Oaks have an intricate Biogeography that criss-crosses diverse climatic and edaphic gradients, encompassing a huge ambiguity in terms of species delimitation. Frequently, the taxonomic proposals brought by traditional Linnaean Botany are either insufficient or rather inflate the number of species and nomenclatural assignments, which are further diluted into inconsistent taxonomic ranks, varying from species to subspecies and varieties. The supremacy given to morphological characters that are inherently fragile and plastic, spread across the distribution areas of distinct lineages, may carry ambiguity on the identification and proper species delimitation. From the oaks that are distributed across the Western Palearctic region, the ones that are deciduous or brevi-deciduous present higher levels of ambiguity in terms of species number and their delimitation. This ambiguity is particularly strong in the circummediterranean region and in the transitional areas between the two major biogeographic Regions of the western Palearctic region, the Euro-Siberian and Mediterranean. This degree of uncertainty, which increases towards the Southern European Peninsulas, is amplified by the ease that the different species of oaks tend to hybridize among them. The present work provides a holistic framework that covers multiple areas, from the taxonomic and evolutive study of this genus, to biogeography and molecular characterization. Its major objective was to resolve the species delimitation of the Iberian deciduous and marcescent oaks and putative introgression among them, enhancing the available knowledge about species diversity, which can foster suitable species and forest conservation. A specific objective was to cross-reference the natural history revision and the different taxonomic treatments brought by distinct authors, with personal observations. These data were then incorporated into ecological modelling and molecular characterization, which in the end fed a newly updated taxonomic proposal. In Section A we obtained results from extensive field, herbaria, and literature review, updating the nomenclature of the Portuguese and western Mediterranean oaks. Section B was supported by Section A’s in-depth review and enabled finer species distribution models, nurturing both hindcast (since ca. 20 Kyr) and forecast (2070-2100) exercises of the range dynamics of Mediterranean oaks species. The study of past and future range shifts solved important pending biogeographic questions, especially related to past range-shifts. Such past-range shifts improved our knowledge on species responses to climate dynamics and allowed a better anticipation of future responses of range shifts driven by climate change. Section C encompassed the molecular characterization of Iberian white oak species and their hybrids, whose delimitation is often faltering when one intends to infer about species rank, or hypothesize about the participation of parent taxon in natural hybrid swarms. This work allowed us to solve the phylogenetic backbone of western Palearctic white oaks, suggesting a significant segregation of the Iberian pedunculate oaks and unveiling two subsections inside Section Quercus. These subsections are biogeographically well-segregated and present diverse levels of introgression among species. Results demonstrated the efficiency of RADSeq for rebuilding the reticulate phylogeny of the Eurasian white oaks, showcasing the significance of the Iberian Peninsula as a major hotspot for oak diversity. We implemented a circular approach to these methods, which retro-fed themselves in terms of insight generation, enabling a powerful strategy to solve the evolutionary history of this difficult groups of plants. We estimate that the reticulate historical biogeography of the western Palearctic white oaks deserves further scrutiny by adding vicariant oak populations from northern Africa, the Near East and southern European Peninsulas. Methods should again follow this similar additive and sequential process of adjoining deep Natural History examination, with extensive fieldwork in type populations and genome-wide molecular surveys, in order to solve this group of plants. With the present work, we were able to significantly improve on the depiction of the basic unit of Biodiversity (the Species), in the complex Quercus genus. We provided tools to enable further efforts for the conservation of the Mediterranean oak forests, which overwhelm one of the most important (and one of the most threatened) Biomes for plant conservation at the global scale.

Research Data Table 1 is a table of the georreferenced historical (literature-based) and species surveys occurrence points for Ctenomys magellanicus (Rodentia, Caviomorpha). These points are the raw data for the analyses performed in this work. Research Data Table 2 is the result of fitting 120 models (EDM) with different features combination.

<h3>Project Overview</h3> <p>Jurisdictional boundaries of governmental agencies often do not align with the geographic or social boundaries of the policy issues they are tasked with addressing. This spatial mismatch is especially common in relation to natural resources and the environment. Where it occurs, achievement of policy goals may require coordination across jurisdictions, which can lead to mutual benefits. Yet, governmental agencies may view coordination as costly or as leading to a loss of autonomy. This project examined coordination decisions made by local level governmental agencies in California, as they formed Groundwater Sustainability Agencies (GSAs) and subsequently coordinated development of their first groundwater sustainability plans (GSPs) under California's Sustainable Groundwater Management Act (SGMA). The project addresses the question of how agencies make decisions and manage interactions when under a coordination mandate that allots agencies the discretion to decide how to coordinate. More specifically, it investigates:<ol><li>What factors influence decisions regarding the geographic extent of and parties involved in development of new formal agencies for groundwater management,</li><li>How do concerns about the potential risks of coordination affect the choice of coordination mechanisms,</li><li>How does the structure of agency interactions affect their achievement of the objectives of the coordination mandate, and</li><li>How do agencies make sense of a coordination mandate and how does that sense-making process influence the decisions agencies make when deciding how to respond to the mandate?</li></ol></p> <h3>Data Collection Overview</h3> <p>Data were collected between January 2018 and May 2020. The methods for data collection varied by data type.<ul><li>Secondary data on the physical, social, and institutional characteristics of groundwater basins were collected from California Department of Water Resources datasets, the American Community Survey, and the National Land Use Database.</li><li>Data on GSA formation and copies of GSPs and Coordination Agreements were obtained from the California’s SGMA Portal Website (https://sgma.water.ca.gov/portal/)</li><li>Meeting minutes and other documentation were obtained from the respective websites of local-level agencies that formed GSAs.</li><li>Interviews were conducted with representatives from 67 groundwater sustainability agencies. Interviewees spanned 17 of the 19 basins and 38 of the 44 groundwater sustainability plans produced. Interviewees were identified based on formal GSA contact information and selected based on formal notices to produce a GSP. Recruitment sought to interview representatives from least one GSA from each GSP group.</li><li>Participant observation was undertaken of more than 58 public meetings (in person, virtually, or reviewing recordings).</li></ul></p> <h3>Shared Data Organization</h3> <p>The shared data is organized into three folders. A GIS folder contains 16 relevant data files. An interview transcripts folder contains 52 de-identified transcripts from the interviews that were recorded and transcribed. Some interviewees did not agree to recording and transcription of the interviews, thus data from those interviews are not available. A tabular data folder contains 3 spreadsheet workbooks. These include a spreadsheet documenting coordination concerns at the basin-level; a spreadsheet documenting organizational forms and institutions adopted at the basin-level; and a spreadsheet documenting coordination outcomes at the basin-level. Each spreadsheet includes a copy of the codebook used in analyzing the data. This data project also includes 6 documentation files: a GIS metadata workbook, an interview catalog, an interview consent form, a redaction protocol, this data narrative, and an administrative README file.</p> <h3>Data Overview</h3> <p>The research involved a mixed-methods approach that combines information on agencies; the physical, social, and institutional characteristics of groundwater basins and the agencies located within them; formal filings; agreements; and plans developed by agencies; meeting minutes; interview data; and data from participant observation.</p>

Ethylene oxide production process is one of the highest energy consumers in chemical industry, and therefore even a slight improvement in its overall efficiency can have a significant impact on the sustainability of the process. Efficiency improvement can be carried out using the exergy-aided pinch analysis outlined in this paper. The overall exergy loss distribution in different unit operations of an ethylene oxide process was first evaluated and mapped out in the form of “visualized exergetic process flowsheet”. An initial analysis of the four main functional blocks of the process showed that the exothermic reaction block contained the largest exergy loss (6043 and 428 kJ/kg of internal and external losses, respectively) which can be reduced by isothermal mixing, as well as increasing reaction temperature and reduction in pressure drop. The absorption block was also estimated to have the second highest contribution with total exergy losses of 3640 kJ/kg which were mainly due to the cooling column. These losses were then recommended to be reduced by improvements in the concentration and temperature gradients along the tower. Following the block-wise analysis, exergy analysis was then carried out for individual unit operations in each block to pinpoint the main sources of thermal exergetic inefficiency. Thermal solutions to reduce losses were also proposed in accordance with the identified sources of inefficiency, leading to a comprehensive list of cold and hot process streams that could be introduced to reduce losses. Finally, pinch analysis was brought into action to estimate the minimum energy requirements, to select utilities, and to design heat exchanger network. Thus, the methodology used in this work took advantage of both exergy and pinch analyses. The combined thermal-exergy-based pinch approach helped to set energy targets so that all the thermal possible solutions supported by exergy analysis were considered, preventing exclusion of any hot or cold process stream with high potential for heat integration during pinch analysis. Results indicated that the minimum cold utility requirement could be reduced from 601.64 MW (obtained via conventional pinch analysis) to 577.82 MW through screening of streams by the combined methodology.

In this study, air concentrations of BaP in two different seasons (winter 2015 and summer 2016) and BaP levels in ground vegetation from Tarragona County were used as control simulations performed with the WRF-CHIMERE air quality modelling system, in order to reproduce the incidence of that hazardous chemical in air and soils. The CTM was validated for the present climatology, showing a good ability to represent air and soil concentrations of BaP over the target domain (petrochemical, chemical, urban and background sites), particularly in the winter. Then, the variation of the BaP concentrations in air and soils were simulated for the time series 1996-2015 and for the climate change scenario RCP8.5 (2031-2050). While an increase is projected for the levels in air, particularly in chemical and remote sites where the variation can go up to 10%, in terms of soil deposition the findings are the opposite, with an evident decrease in soil BaP concentrations, particularly for background sites. Finally, a potential health effect of BaP for the local population (lung cancer) was assessed. Although according to the projections the EU threshold for BaP atmospheric incidence (1 ng m-3) will not be reached by 2050, there will be an increase in the life-time risk of lung cancer, particularly in the most populated areas within the simulation domain.

The drought prone North-West Bangladesh is vulnerable to the impacts of climate change, particularly because of less water availability in the dry period and high water requirement for crop production. Improved understanding of recent changes in crop water demand in the dry season is important for the water resources management in the region. A study was carried out to determine the potential impacts of recent climate change during last three decades on trends of water requirements of Boro rice. The reference crop evapotranspiration (ETo), potential crop water requirement (∑ETC), effective rainfall during the crop growing period (ER), potential irrigation requirement for crop evapotranspiration (∑ETC − ER) and net irrigation requirement of Boro rice were estimated using observed daily climate data in the CropWat model for the period of 1980 to 2013 for four North-West districts. Significant decreasing trends of ETo were observed in most of the dry months due to increasing relative humidity and decreasing wind-speed and sun-shine hours. The results showed decreasing trends of potential crop water requirement, i.e. the total crop evapotranspiration (∑ETC), of Boro rice due to decreasing reference crop evapotranspiration and shorter crop growing periods. The variations in trends of potential irrigation requirement for crop evapotranspiration (∑ETC − ER) found among different districts, are mainly linked to variations in trends of changes in effective rainfall. The net irrigation requirement of Boro rice has decreased, by 11% during the last three decades at an average rate of −4.4 mm year−1, instead of decreasing effective rainfall, mainly because of high rate of decrease of crop evapotranspiration (−5.9 mm year−1). Results indicate that a warming climate does not always result in higher agricultural water use and that climate change can also result in reduced water demands because of changes in humidity, wind-speed and sun-shine hours.

The transition to a low carbon future is starting to affect landscapes around the world. In order for this landscape transformation to be sustainable, renewable energy technologies should not cause critical trade-offs between the provision of energy and that of other ecosystem services such as food production. This literature review advances the body of knowledge on sustainable energy transition with special focus on ecosystem services-based approaches and methods. Two key issues emerge from this review: only one sixth of the published applications on the relation between renewable energy and landscape make use of the ecosystem service framework. Secondly, the applications that do address ecosystem services for landscape planning and design lack efficient methods and spatial reference systems that accommodate both cultural and regulating ecosystem services. Future research efforts should be directed to further advancing the spatial reference systems, the use of participatory mapping and landscape visualizations tools for cultural ecosystem services and the elaboration of landscape design principles.

Since 2007, a range of new modeling approaches and tools have been developed for sustainability impact assessment (SIA), but a lack of universal acceptance of SIA tools in applied policy making is observed. The current article gives an overview of experiences from several European and international projects, critically reviews the selected SIA tools and then discusses a number of reasons for the observed disconnect of the tools with the potential users. Largely based on the experiences of the presented SIA tools focusing land use policy advice, a decision tree is designed, which may facilitate an adequate, region and developmental phase specific selection of tool-box components for the development of SIA tools in future. Elements to ensure end-user participation are also integrated in the decision tree in order to increase the likelihood of the tool in applied land use policy advice. In addition, the Challenges in SIA tool development and use are further discussed.