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Digital Annex for the following thesis: Vila-Viçosa, C. (2023). Natural History, Biogeography and Evolution of the Iberian white oak syngameon (Quercus L. Sect. Quercus). Ph.D. Thesis, Faculdade de Ciências da Universidade do Porto, Portugal Abstract: The genus Quercus L. is one of the most diverse and important group of woody plants, particularly when considering that they are the trees that rule the Northern Hemisphere forests. Oaks have an intricate Biogeography that criss-crosses diverse climatic and edaphic gradients, encompassing a huge ambiguity in terms of species delimitation. Frequently, the taxonomic proposals brought by traditional Linnaean Botany are either insufficient or rather inflate the number of species and nomenclatural assignments, which are further diluted into inconsistent taxonomic ranks, varying from species to subspecies and varieties. The supremacy given to morphological characters that are inherently fragile and plastic, spread across the distribution areas of distinct lineages, may carry ambiguity on the identification and proper species delimitation. From the oaks that are distributed across the Western Palearctic region, the ones that are deciduous or brevi-deciduous present higher levels of ambiguity in terms of species number and their delimitation. This ambiguity is particularly strong in the circummediterranean region and in the transitional areas between the two major biogeographic Regions of the western Palearctic region, the Euro-Siberian and Mediterranean. This degree of uncertainty, which increases towards the Southern European Peninsulas, is amplified by the ease that the different species of oaks tend to hybridize among them. The present work provides a holistic framework that covers multiple areas, from the taxonomic and evolutive study of this genus, to biogeography and molecular characterization. Its major objective was to resolve the species delimitation of the Iberian deciduous and marcescent oaks and putative introgression among them, enhancing the available knowledge about species diversity, which can foster suitable species and forest conservation. A specific objective was to cross-reference the natural history revision and the different taxonomic treatments brought by distinct authors, with personal observations. These data were then incorporated into ecological modelling and molecular characterization, which in the end fed a newly updated taxonomic proposal. In Section A we obtained results from extensive field, herbaria, and literature review, updating the nomenclature of the Portuguese and western Mediterranean oaks. Section B was supported by Section A’s in-depth review and enabled finer species distribution models, nurturing both hindcast (since ca. 20 Kyr) and forecast (2070-2100) exercises of the range dynamics of Mediterranean oaks species. The study of past and future range shifts solved important pending biogeographic questions, especially related to past range-shifts. Such past-range shifts improved our knowledge on species responses to climate dynamics and allowed a better anticipation of future responses of range shifts driven by climate change. Section C encompassed the molecular characterization of Iberian white oak species and their hybrids, whose delimitation is often faltering when one intends to infer about species rank, or hypothesize about the participation of parent taxon in natural hybrid swarms. This work allowed us to solve the phylogenetic backbone of western Palearctic white oaks, suggesting a significant segregation of the Iberian pedunculate oaks and unveiling two subsections inside Section Quercus. These subsections are biogeographically well-segregated and present diverse levels of introgression among species. Results demonstrated the efficiency of RADSeq for rebuilding the reticulate phylogeny of the Eurasian white oaks, showcasing the significance of the Iberian Peninsula as a major hotspot for oak diversity. We implemented a circular approach to these methods, which retro-fed themselves in terms of insight generation, enabling a powerful strategy to solve the evolutionary history of this difficult groups of plants. We estimate that the reticulate historical biogeography of the western Palearctic white oaks deserves further scrutiny by adding vicariant oak populations from northern Africa, the Near East and southern European Peninsulas. Methods should again follow this similar additive and sequential process of adjoining deep Natural History examination, with extensive fieldwork in type populations and genome-wide molecular surveys, in order to solve this group of plants. With the present work, we were able to significantly improve on the depiction of the basic unit of Biodiversity (the Species), in the complex Quercus genus. We provided tools to enable further efforts for the conservation of the Mediterranean oak forests, which overwhelm one of the most important (and one of the most threatened) Biomes for plant conservation at the global scale.

The Coast to Karoo Transect investigates the abundance and diversity of ants and beetles along an altitudinal gradient in the Cederberg mountains of the Western Cape, South Africa. It is a long term project, initiated in 2002 by Prof. S.L. Chown, Stellenbosch University. Data collection is carried out on a biannual (spring and autumn) basis. To monitor changes in invertebrate assemblages, focusing on ants and beetles.

The aim of this report is to demonstrate and evaluate the potential of tall wheatgrass (Elytrigia elongata) to avoid GHG emissions and obtain lower economic costs in marginal areas of Spain. Our research built scenarios based on experimental plots (2 and 3 years growth) in 3 locations of Spain with completely different climate conditions (provinces of Girona, Soria and Palencia). In our experiences, we achieved an adequate establishment and biomass production, and assumed a rank of biomass yields until the end of the life cycle that is usually accepted to be about 15 years in many other studies in United States, Argentina and Eastern Europe where tall wheatgrass is extensively cultivated in marginal areas for sheep livestock production. Using our experimental plots and statistical information for economic inputs costs, we built 5 different scenarios per region considering a large range of biomass yields of tall wheatgrass. The analysis included a comparison with annual grasses economic costs calculated for a wide range of biomass yields of a previous study. We estimated GHG emissions savings for tall wheatgrasses and used our previous study (which had GHG emissions savings as well). Savings were calculated replacing natural gas electricity with electricity from biomass combustion in real power plants in Spain. In a wide range of yields, the results suggest that marginal areas might present a better performance with tall wheatgrass compared to annual winter grasses (cereals whole plant cuttings), thus producing biomass yields with higher GHG savings and lower economic costs at the farm level. Proceedings of the 20th European Biomass Conference and Exhibition, 18-22 June 2012, Milan, Italy, pp. 217-229

It is recognised that several constraints such as the lack of knowledge and expertise of farmers, land users and policy makers concerning agroforestry systems establishment and management hamper the adoption of agroforestry systems (Camilli et al. 2017). AFINET project acts at EU level in order to direct research results into practice and promote innovative ideas to face challenges and solve practitioners' problems. AFINET proposes an innovative methodology based on the creation of a European Interregional Network, linking different Regional Agroforestry Innovation Networks (RAINs). RAINs represent different climatic, geographical, social and cultural conditions and enclose a balanced representation of the key actors with complementary types of expertise (farmers, policy makers, advisory services, extension services, etc.). The Italian RAIN is focused on the Extra-Virgin Olive Oil (EVOO) value chain, with the main aim to promote agroforestry management of local olive orchards. Olive trees are still managed traditionally, often in marginal sites, with minimal mechanization and relatively low external inputs such as chemical treatments in comparison to other crops. The presence of permanent crops (olive trees) guarantees a partially tree cover reducing hydrogeological risk. Soil management usually keeps natural grassing reducing soil carbon emission and increasing soil fertility (Bateni et al. 2017). Intercropping with cereals and/or fodder legumes and livestock can also be practiced in olive orchards, increasing the complexity of the olive tree multifunctional system. Moreover, olive orchards can be managed as agroforestry systems since they can be intercropped with arable crops (cereals, legumes) and/or combined with livestock (sheep, poultry). The RAIN process, involving local stakeholders, highlighted the main bottlenecks of the EVOO value chain related to communication and dissemination of knowledge, technical and management aspects, market and policy. In order to contrast bottlenecks and exploit opportunities of the olive oil supply chain, the identified innovations are: i) adoption of best practices: testing and experimenting innovative agroforestry systems introducing different crop/animals species and varieties; ii) improve the management of the olive orchards: encouraging and increasing the organic production; iii) valorisation of olive processing residues: identifying and testing innovative products (bio-materials, olive paste as example); iv) arise the awareness among consumers: educating people about the benefits of olive oil consumption, creating networks among stakeholders, improving marketing and commercialization. Creating a Bio-district, defined as a geographical area where farmers, citizens, tourist operators, associations and public authorities enter into an agreement for the sustainable management of local resources, emerged a powerful tool to implement the innovation in the local EVOO value chain.

Treball Final de Grau en Finances i Comptabilitat. Codi: FC1049. Curs acadèmic: 2019/2020 In recent years there has been a rise in businesses investing in social and ethical issues. The sustainable development goals published in 2015 by the UN provide a globally agreed framework to make the world more sustainable. Companies have been given the opportunity to help the environment and society through their actions whilst increasing their value and return and improving their reputation. A method for doing so is by following the principles of responsible investment and incorporating environmental, social and governance criteria into long-term businesses strategies. Spanish banks are actively working to achieve the sustainable development goals by investing in sustainable finance, social projects, housing programmes, etc. to meet societal needs whilst extending their market scope and achieving corporate growth and financial success. The main focus of their actions lays with two specific goals: ending poverty and providing decent work and economic growth for all.

Glacial extent and mass balance are sensitive climate proxies providing solid information on past climatic conditions. However, series of annual mass-balance measurements of more than 60 years are scarce. To our knowledge, this is the first time the latewood density data (MXD) of the Swiss stone pine (Pinus cembra L.) have been used to reconstruct the summer mass balance (B s ) of an Alpine glacier. The MXD-based B s well correlates with a B s reconstruction based on the May to September temperature. Winter precipitation has been used as an independent proxy to infer the winter mass balance and to obtain an annual mass-balance (B n ) estimate dating back to the gla- ciological year 1811/12. The reconstructed MXD/precipitation-based B n well correlates with the data both of the Careser and of other Alpine glaciers measured by the glaciological method. A number of critical issues should be considered in both proxies, including non-linear response of glacial mass balance to temperature, bedrock topography, ice thinning and fragmentation, MXD acquisition and standardization methods, and finally the 'divergence problem' responsible for the recently reduced sensitivity of the dendrochronological data. Nevertheless, our results highlight the possibility of performing MXD-based dendroglaciological reconstructions using this stable and reliable proxy.

Introduction The European Union aims at raising the share of energy consumption produced from renewable resources to 20% in 2020 as compared to 1990. Moreover, the European Commission adopted a strategy called "Innovating for Sustainable Growth: a Bioeconomy for Europe" to shift the European economy towards greater and more sustainable use of renewable resources. The S2Biom project (www.s2biom.eu) - Delivery of sustainable supply of non-food biomass to support a "resource-efficient" Bioeconomy in Europe - supports the sustainable delivery of non-food biomass feedstock at local, regional and pan-European levels through developing strategies and roadmaps that will be render available to the user by a "computerized and easy to use" toolset (and respective databases) with updated harmonized datasets at local, regional, national and pan-European level for EU-28, Western Balkans, Moldova, Turkey and Ukraine. Methodology Taking in consideration the results and experiences of current and past EU projects, the S2Biom project activities are implemented in three individual but strongly interrelated Themes: 1) To focus on methodological approaches, data collection and estimation of sustainable biomass potentials resources, efficient pathways and optimal logistical supply routes as well as the development of a computerised toolset. 2) To make use of the findings of Theme 1 and develop a Vision, strategies and a R&D roadmap for sustainable delivery of non-food biomass feedstock at local, regional and pan- European levels. 3) To validate the results from themes 1 and 2 and ensure the project outreach; this will be performed through selected case studies which will efficiently capture the different scales of applications for biomass supply chains in a sufficient number of regions across Europe. Conclusion The first draft version of this toolset have been developed, allowing to analyse different levels of bioenergy production, its costs and feedstock biomass crops available across EU at a resolution level of Nuts3.

The energy crop sweet sorghum (Sorghum bicolor L. Moench) is raising considerable interest as a source of either fermentable free sugars or lignocellulosic feedstock with the potential to produce fuel, food, feed and a variety of other products. Sweet sorghum is a C4 plant with many potential advantages, including high water, nitrogen and radiation use efficiency, broad agro-ecological adaptation as well as a rich genetic diversity for useful traits. For developing countries sweet sorghum provides opportunities for the simultaneous production of food and bioenergy (e.g. bio-ethanol), thereby contributing to improved food security as well as increased access to affordable and renewable energy sources. In temperate regions (e.g. in Europe) sweet sorghum is seen as promising crop for the production of raw material for 2nd generation bio-ethanol. The project SWEETFUEL (Sweet Sorghum: An alternative energy crop) is supported by the European Commission in the 7th Framework Programme to exploit the advantages of sweet sorghum as potential energy crop for bio-ethanol production. Thereby, the main objective of SWEETFUEL is to optimize yields in temperate and semi-arid regions by genetic enhancement and the improvement of cultural and harvest practices. Proceedings of the 18th European Biomass Conference and Exhibition, 3-7 May 2010, Lyon, France, pp. 200-206

The exploitation of forest biomass may offer important benefits in terms of environmental protection and local employment, but the development of an effective forest biomass chain is hindered by the lack of techniques for tapping the resource at reasonable costs.