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We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures. Empty cells = NAFurther Project information: Core Facility grant; Award: GE 1775/2-1

DataForAssessingSeasonalEffectsOnElectricityAndWaterForRepositoryThis Excel file contains data used to conduct a seasonal analysis to assess whether seasonal patterns exist in electricity use in dorms and whether these patterns differ by latitude. The first worksheet contains metadata.Fall 2010 Campus Conservation Nationals surveyThis online survey was administered to students attending colleges who participated in the Fall 2010 Campus Conservation Nationals competition. Not all schools who participated in the competition administered the survey.CCN_F10_survey.pdfSpring 2012 Campus Conservation Nationals surveyThis online survey was administered to students attending colleges who participated in the Spring 2012 Campus Conservation Nationals competition. Not all schools who participated in the competition administered the survey.CCN_Spring12_survey.pdfFall 10 Campus Conservation Nationals electricity, water, webhit, and commitment dataThis data file contains data at the dorm level collected by Lucid before, during, and after the Fall 2010 CCN competition. The first sheet contains metadata defining all variable names.Fall10_CCN_elec_water_webhits_commitments.xlsxSpring 2012 Campus Conservation Nationals electricity, water, and commitment dataThis data file contains data at the dorm level collected by Lucid before, during, and after the Spring 2012 CCN competition. The first sheet contains metadata defining all variable names.Spring12_CCN_elec_water_commitments_no.xlsxFall 10 CCN data aggregated at dorm level with psychological variablesThis data file contains data at the dorm level collected from our online survey and merged with the resource use data. The first sheet contains metadata defining all variable names.Fall10_CCN_dormagg_with_psych_variables.xlsxSpring 2012 CCN data with psychological variablesThis data file contains data at the dorm level collected from our online survey and merged with the resource use data. The first sheet contains metadata defining all variable names.Spring12__CCN_dormagg_with_psych_variables.xlsx “Campus Conservation Nationals” (CCN) is a recurring, nation-wide electricity and water-use reduction competition among dormitories on college campuses. We conducted a two year empirical study of the competition’s effects on resource consumption and the relationship between conservation, use of web technology and various psychological measures. Significant reductions in electricity and water use occurred during the two CCN competitions examined (n = 105,000 and 197,000 participating dorm residents respectively). In 2010, overall reductions during the competition were 4% for electricity and 6% for water. The top 10% of dorms achieved 28% and 36% reductions in electricity and water respectively. Participation was larger in 2012 and reductions were slightly smaller (i.e. 3% electricity). The fact that no seasonal pattern in electricity use was evident during non-competition periods suggests that results are attributable to the competition. Post competition resource use data collected in 2012 indicates that conservation behavior was sustained beyond the competition. Surveys were used to assess psychological and behavioral responses (n = 2,900 and 2,600 in 2010 and 2012 respectively). Electricity reductions were significantly correlated with: web visitation, specific conservation behaviors, awareness of the competition, motivation and sense of empowerment. However, participants were significantly more motivated than empowered. Perceived benefits of conservation were skewed towards global and future concerns while perceived barriers tended to be local. Results also suggest that competitions may be useful for “preaching beyond the choir” – engaging those who might lack prior intrinsic or political motivation. Although college life is distinct, certain conclusions related to competitions, self-efficacy, and motivation and social norms likely extend to other residential settings.

We simulated an experimental summer storm in large-volume (~1200 m3, ~16m depth) enclosures in Lake Stechlin by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Copepod and Cladocera biomass was monitored for 42 days after the experimental disturbance event (Utermöhl counting at 60x magnification and biomass calculation from length-dry mass relationships). Sampling was performed using a 90 µm mesh size Apstein-cone. Method: 90 µm mesh size Apstein-cone, Utermöhl counting at 60x, TSO image analysis program (KIO VIDMESS-2011, Version 2.60.50, including a TSO-KST1003832 camera, TSO - Thalheim-Spezial-Optik-Gerätebau, Pulsnitz, Germany), biomass calculations from length-dry mass relationships (Bottrell et al. 1976; Kasprzak 1983).Further Project information:Core Facility grant; Award: GE 1775/2-1

At each station, seawater was collected for trace metals sampling at 20 m depth using a Teflon pump (Furon®) with a 40 m Teflon tube connected with a AcroPakTM 1500 capsule w/Supor Memb 0.8/0.2 μm filter. The pump was left to pump to rinse the inner hose and 1 L sample was collected in LDPE bottles (NALGENE) and stored at -20oC until the land-based laboratory analysis. The material was previously cleaned following the trace metals GEOTRACES protocol and the experiments were done in Class 100 clean laboratory or trace metal analysis (QUIMA- IOCAG TM lab).

A set of environmental variables have been collected for a period of 7 consecutive years (2016-2022, from April/May to September/October, during the glacial melting season) along a longitudinal transect of the Saldur stream, a near pristine glacier-fed stream located in South Tyrol, Italy (46°N, 10° E), part of the International Long Term Ecological Research (ILTER) network (site code: LTER_EU_IT_100). From year 2016 on, a small run-of-river (ROR) hydropower plant (max. 3,200 kW/year) started to operate on the stream, located in close proximity to site Saldur_2A. The potential impact on the riverine ecosystem was largely investigated, and resulted to be negligible. Extensive details on this topic can be found reading the scientific publications: Scotti et al. (2022; doi:10.3389/fenvs.2022.902603) and Scotti et al. (2022; doi:10.3389/fenvs.2022.904547).The environmental variables measured (as spot-measurement) were: suspended solids (mL/L); specific conductance at 25° C (mS/cm), water temperature (°C), calculation of the bottom component of the Pfankuch index. Whilst only in 2018 turbidity (NFU) was measured instead of suspended solids, and all the measurements were conducted using a multiparameter meter (HI9829, Hanna Instruments), for all the remaining years suspended solids were measured after 30 minutes of water sedimentation in a Imhoff cone, and specific conductance through a portable conductimeter (Cond 7, XS Instruments). More methodological details can be found in Scotti & Bottarin (2021; doi:10.1038/s41597-021-00887-x).

The data describes plant gas exchange dynamics (CO2, H2O) together with online proton transfer reaction mass spectrometry measurements of biogenic volatile organic compound emissions of Pinus halepensis seedlings exposed to two similar heatwaves together with drought and a recovery period. Measured in a scientific glasshouse facility at KIT IMK-IFU Garmisch-Partenkirchen, Germany, via an automated chamber setup. #0 means "Inf"

Figure 1data_Exp 2Figure 1 data: Condition of experimental seedlings in hummocks with contrasting shrub density and tree canopy in Experiment 2: No Trees - Low Shrub biomass (NTLS), No Trees - High Shrub biomass (NTHS), Present Trees - Low Shrub biomass (PTLS) and Present Trees - High shrub biomass (PTHS) during the warmest growing season (2011) and at the end of the experiment (2013). Seedling condition was defined as: healthy (< 50% of the needles turned yellow or brown) or unhealthy (> 50% of the needles turned yellow or brown). Seedlings were 1 month old at plantation time in the July 2010.Table 1_environmental conditions_Exp 1Table 1 data: Environmental conditions and vegetation characteristics in hummocks (circular and bands) and lawns for Experiment 1. Water table depth below surface is an average for the four growing seasons (2010-2013)Table 2_ photosynthesis data_Exp 1Table 2 photosynthesis data: Photosynthesis rates for experimental pine seedlings in hummocks (circular and bands) versus adjacent lawns for Experiment 1.Table 2_seedling responses_Exp 1Table 2 data: Responses of experimental pine seedlings in hummocks (circular and bands) versus adjacent lawns for Experiment 1 after 4 growing seasons. ST: Seeds inserted on top of moss; SB: Seeds inserted below moss; Small seedling (1 month old at plantation time); Large seedling (2 months old at plantation time). Emergence = % of planted seeds emerged after 1 year. Condition = % healthy seedlings. Stem growth corresponds to vertical stem growth for germinating (ST and SB) seedlings and new stem growth for older (small and large) seedlings.Table 3_regression seedling-environment_Exp 1Table 3 data for generalized linear models assessing the responses of experimental pine seedlings in hummocks (circular and bands) and adjacent lawns for Experiment 1 during the whole experimental period (2010-2013). ST: Seedlings from seeds inserted on top of moss; SB: Seedlings from seeds inserted below moss; Small seedling (1 month old at plantation time); Large seedling (2 months old at plantation time). Condition = % healthy seedlings. Growth = stem growth.Table 4_Environmental data_Exp 2Table 4: Environmental conditions in hummocks with contrasting shrub density and tree canopy in Experiment 2: No Trees - Low Shrub biomass (NTLS), No Trees - High Shrub biomass (NTHS), Present Trees - Low Shrub biomass (PTLS) and Present Trees - High shrub biomass (PTHS).Table 4 and Table S5a_seedling performance_Exp 2Table 4: Seedling performance in hummocks with contrasting shrub density and tree canopy in Experiment 2: No Trees - Low Shrub biomass (NTLS), No Trees - High Shrub biomass (NTHS), Present Trees - Low Shrub biomass (PTLS) and Present Trees - High shrub biomass (PTHS). Seedling emergence, condition and survival from seeds inserted below the moss (SB), and from small planted seedlings.Table S3_cox regression (survival analysis)_Exp 1Table S3: Data for Cox survival analysis for experimental pine seedlings in hummocks (circular and bands) versus adjacent lawns during 2010-2013. ST: Seedlings from seeds inserted on top of moss; SB: Seedlings from seeds inserted below moss; Small seedling (1 month old, 10 cm tall at plantation time); Large seedling (2 months old, 30 cm tall at plantation time).Table S4_ regression seedling-environment 2011_Exp 1Table S4: Data for generalized linear models assessing the responses of experimental pine seedlings in hummocks (circular and bands) and adjacent lawns for Experiment 1 in 2011. Small seedling (1 month old, 10 cm tall at plantation time); Large seedling (2 months old, 30 cm tall at plantation time). Condition = % healthy seedlings. Growth = stem growth. Boreal ecosystems are warming roughly twice as fast as the global average, resulting in woody expansion that could further speed up the climate warming. Boreal peatbogs are waterlogged systems that store more than 30% of the global soil carbon. Facilitative effects of shrubs and trees on the establishment of new individuals could increase tree cover with profound consequences for the structure and functioning of boreal peatbogs, carbon sequestration and climate. We conducted two field experiments in boreal peatbogs to assess the mechanisms that explain tree seedling recruitment and to estimate the strength of positive feedbacks between shrubs and trees. We planted seeds and seedlings of Pinus sylvestris in microsites with contrasting water-tables and woody cover and manipulated both shrub canopy and root competition. We monitored seedling emergence, growth and survival for up to four growing seasons and assessed how seedling responses related to abiotic and biotic conditions. We found that tree recruitment is more successful in drier topographical microsites with deeper water-tables. On these hummocks, shrubs have both positive and negative effects on tree seedling establishment. Shrub cover improved tree seedling condition, growth and survival during the warmest growing season. In turn, higher tree basal area correlates positively with soil nutrient availability, shrub biomass and abundance of tree juveniles. Synthesis. Our results suggest that shrubs facilitate tree colonization of peatbogs which further increases shrub growth. These facilitative effects seem to be stronger under warmer conditions suggesting that a higher frequency of warmer and dry summers may lead to stronger positive interactions between shrubs and trees that could eventually facilitate a shift from moss to tree-dominated systems.