The dataset CO2_flux_measurements_Acoculco contains data on CO2 fluxes, coordinates (UTM), air temperature, atmospheric pressure measured in selected sites belonging to the Acoculco Geothermal Field: in particular, the areas named Lagunilla, Alcaparrosa, Los Azufres and also the area between them were investigated. CO2 flux measurements were performed using the accumulation chamber method. The dataset Field_meas_Acoculco_waters reports the ID, coordinates (UTM), Altitude (m.a.s.l.), temperature, flow rate, pH, Electrical Conductivity and Dissolved Oxygen for water samples collected in the central sector of the Acoculco geothermal field, but also in other sectors located inside and outside the Acoculco caldera. Total depth is also included for samples collected from water wells. The dataset Chemical_isotopic_data_Acoculco_waters reports major and minor chemical components and stable isotopic composition for hydrogen and oxygen determined in collected water samples in Acoculco geothermal field. Calculated partial pressures (in bars and log10-value) and CO2 concentrations of dissolved CO2 were also included. The dataset Chemical_isotopic_data_Acoculco_gas reports chemical and isotopic data for collected samples from Los Azufres and Alcaparrosa natural gas manifestations.