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Estuaries are among the most valuable aquatic systems by their services to human welfare. However, increasing human activities at the watershed along with the pressure of climate change are fostering the co-occurrence of multiple environmental drivers, and warn of potential negative impacts on estuaries resources. At present, no clear understanding of how coastal ecosystems will respond to the non-stationary effect of multiple drivers. Here we analysed the temporal interaction among multiple environmental drivers and their changing priority on shaping phytoplankton response in the Bahía Blanca Estuary, SW Atlantic Ocean. The interaction among environmental drivers and the number of significant direct and indirect effects on chlorophyll concentration increased over time in concurrence with enhanced anthropogenic stress, changing winter climate and wind patterns. Over the period 1978-1993, proximal variables such as nutrients, water temperature and salinity, showed a dominant effect on chlorophyll, whereas in more recent years (1993-2009) climate signals (SAM and ENSO) boosted indirect effects through its influence on precipitation, wind, water temperature and turbidity. Turbidity emerged as the dominant driver of chlorophyll while in recent years acted synergistically with the concentration of dissolved nitrogen. As a result, chlorophyll concentration showed a significant negative trend and a loss of seasonal peaks reflecting a pronounced reorganisation of the phytoplankton community. We stress the need to account for the changing priority of drivers to understand, and eventually forecast, biological responses under projected scenarios of global anthropogenic change.

Available methods for measuring the impact of ocean acidification (OA) and leakage from carbon capture and storage (CCS) on marine sedimentary pH profiles are unsuitable for replicated experimental setups. To overcome this issue, a novel optical sensor application is presented, using off-the-shelf optode technology (MOPP). The application is validated using microprofiling, during a CCS leakage experiment, where the impact and recovery from a high CO2 plume was investigated in two types of natural marine sediment. MOPP offered user-friendliness, speed of data acquisition, robustness to sediment type, and large sediment depth range. This ensemble of characteristics overcomes many of the challenges found with other pH measuring methods, in OA and CCS research. The impact varied greatly between sediment types, depending on baseline pH variability and sediment permeability. Sedimentary pH profile recovery was quick, with profiles close to control conditions 24 h after the cessation of the leak. However, variability of pH within the finer sediment was still apparent 4 days into the recovery phase. Habitat characteristics need therefore to be considered, to truly disentangle high CO2 perturbation impacts on benthic systems. Impacts on natural communities depend not only on the pH gradient caused by perturbation, but also on other processes that outlive the perturbation, adding complexity to recovery.

Elevated environmental carbon dioxide (pCO2) levels have been found to cause organ damage in the early life stages of different commercial fish species, including Atlantic cod (Gadus morhua). To illuminate the underlying mechanisms causing pathologies in the intestines, the kidney, the pancreas and the liver in response to elevated pCO2, we examined related gene expression patterns in Atlantic cod reared for two months under three different pCO2 regimes: 380 μatm (control), 1800 μatm (medium) and 4200 μatm (high). We extracted RNA from whole fish sampled during the larval (32 dph) and early juvenile stage (46 dph) for relative expression analysis of 18 different genes related to essential metabolic pathways. At 32 dph, larvae subjected to the medium treatment displayed an up-regulation of genes mainly associated with fatty acid and glycogen synthesis (GYS2, 6PGL, ACoA, CPTA1, FAS and PPAR1b). Larvae exposed to the high pCO2 treatment upregulated fewer but similar genes (6PGL, ACoA and PPAR1b,). These data suggest stress-induced alterations in the lipid and fatty acid metabolism and a disrupted lipid homeostasis in larvae, providing a mechanistic link to the findings of lipid droplet overload in the liver and organ pathologies. At 46 dph, no significant differences in gene expression were detected, confirming a higher resilience of juveniles in comparison to larvae when exposed to elevated pCO2 up to 4200 μatm.

This article outlines issues to be tackled when considering increases in biofuel usage in the European Union (EU) and examines a potential scheme to increase the use of biofuels in the road transport sector; the development of biofuels corridors. An EU biofuels corridor is defined as a long-distance and cross-border route on the Trans-European Transport (TEN-T) Network roads on which blends with a high biofuel content (referred to as high blends) are offered at regular intervals along the entire route. The article first defines the current framework of EU biofuels development. A case study on the feasibility of one possible EU biofuels corridor, from Rotterdam, Netherlands, to Constanta, Romania, is analyzed along four potential biofuels corridor designs (under different future scenarios). The case study includes interviews with key stakeholders, transport flows analysis, refueling infrastructure, and biofuels policy in the relevant member states. The results are extrapolated to the complete EU level in order to assess the potential effect of the biofuels corridor approach as a measure of stimulating the use of biofuels. It is concluded that EU biofuels corridors can increase the use of biofuels. However, if applied as a stand-alone measure a maximum contribution is limited. The effectiveness of biofuels corridors is not larger mainly due to the fact that the transport flows on the TEN-T Network roads are not representative of actual fuel sales at stations on this network (i.e., motorway stations). In addition, various recommendations are made for further research. © 2012 American Society of Civil Engineers.

Fleets of commercial vehicles for delivery services in urban areas constitute road transportation means which are required to run relatively short distances and to respect anti-pollution laws commonly imposed by many municipalities. For this kind of commercial applications, high efficiency and eco-friendly electric propulsion systems offer an interesting alternative to thermal engines. This paper is focused on the analysis of such solution, by presenting experimental results obtained with a ZEBRA battery based propulsion system, designed to power a specific urban unit within the category of electric commercial vehicles. A novel contribution is added to the relevant literature concerning battery based electric powertrains for road vehicles. The main novelty consists in a wide range of experimental results and performance analysis carried out with reference to the real behavior of both the whole propulsion system and each main component, when powering the commercial vehicle, on the urban part of the NEDC (New European Driving Cycle) standard driving cycle, at different slopes. The experimental results, expressed through electrical and mechanical parameters, are initially evaluated by means of a quasi-static numerical model of the electric powertrain and then experimentally verified with the support of a 1:1 scale laboratory dynamic test bench. The procedure followed and presented in this paper definitely demonstrates the good design and performance, obtained for the evaluated propulsion system, in satisfying the real energy and power requirements, specific of an urban use for delivery commercial vehicles, in terms of daily autonomy and slopes. The collections of experimental results, analyzed in the paper, represent in addition a useful set of data for simulation in order to build, verify and improve models in their outputs.

An integrated software tool environment is presented, and a methodology is proposed for the operational support of the local authority, for analysis of the impact of transport measures in terms of network energy consumption and pollutant emissions. It is based on work done by the European Union within the save program (speci®c actions for vigorous energy eciency)ÐSlam project (supporting local authorities methodology). As background, the Slam project is described, with the principal aspects and needs of environmental and trac network management. The central section de®nes a methodology able to support technicians in recognizing the trac asset and decision makers in evaluating interventions on urban transport infrastructures or technological systems. The role of the di€erent models and their interactions with the transport telematics services currently active on the Florence (Italy) network is discussed. Finally, the procedure for calculating the trac impacts on energy consumption is described with the help of a test case, the evaluation of a dedicated bus corridor in Florence. # 1999 Published by Elsevier Science Ltd. All rights reserved.

Myanmar's fisheries are among the most important globally but remain some of the least documented. The fisheries sector occupies an important place in Myanmar's economy and culture, and is set to change rapidly as the country enters a period of unprecedented political and economic transition. Building on a unique set of information sources, this article presents a broad view of the current state of knowledge on governance, livelihoods, production and supply chains across Myanmar's three main fishery sub-sectors (marine capture, inland capture, and aquaculture). The analysis is contextualized with a review of major changes in the country's policy history affecting fisheries. It is argued that Myanmar's fisheries now sit at a potential cross-road in terms of their governance. Taking advantage of Myanmar's latecomer position in its current transition, this article draws parallels with regional experiences to outline sectoral recommendations for policy reform.

Abstract Monitoring operations at the seafloor above a storage complex area are required to demonstrate that offshore storage of CO2 in the deep subsurface is safe and effective. Within the framework of the STEMM-CCS project, the Goldeneye area was identified as an offshore experimental site for CO2 storage. In this work, the physico-chemical characteristics of the water column at the Goldeneye site were determined by an in-depth analysis of measurements collated from CTD casts, discrete water samples and seafloor landers. The results showed a clear tidal, seasonal and inter-annual variability in the measured parameters within the monitoring period (Oct 2017- May 2019). Variations in pH and pCO2 over a single tidal cycle were in the order of ±0.008 and ±1.5 μatm, respectively. The temporal variability of water column carbonate chemistry parameters was further defined with the aim to provide a solid background for the discrimination from natural changes of potential impacts of CO2 emissions at the potential storage site. Here, we demonstrated how the combination of new and existing methods and technologies could be used for an effective assessment of water column conditions at a potential offshore carbon storage site.