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Optimizing liquid fermentation for Wolfiporia cocos: gene expression and biosynthesis of pachymic acid and mycelial biomass
descriptionPublicationkeyboard_double_arrow_right Article 2024Publisher:Oxford University Press (OUP)
Authors: Hamza Armghan, Noushahi; Aamir Hamid, Khan; Hamza Ali, Khan; Marcin, Kiedrzyński; +5 Authors
Hamza Armghan, Noushahi; Aamir Hamid, Khan; Hamza Ali, Khan; Marcin, Kiedrzyński; Adnan, Akbar; Raheel, Shahzad; Sri, Koerniati; Abdulwahed Fahad, Alrefaei; Shaohua, Shu;
doi: 10.1093/lambio/ovae054
pmid: 38866707
doi: 10.1093/lambio/ovae054
pmid: 38866707
Abstract Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine, holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic acid (PA) concentration, and overall PA production. Additionally, we investigated the molecular basis of our findings by quantifying the expression levels of genes associated with PA and MB biosynthesis using quantitative real-time polymerase chain reaction. Under the optimized fermentation conditions, significant results were achieved, with maximum MB reaching 6.68 g l−1, PA content peaking at 1.25 mg g−1, and a total PA yield of 4.76 g l−1. Notably, among the four examined genes, squalene monooxygenase, exhibited enhanced expression at 0.06 ratio under the optimized conditions. Furthermore, within the realm of carbohydrate-active enzymes, the glycoside hydrolases 16 family displayed elevated expression levels at 21 ratios, particularly during MB production. This study enhances understanding of genetic mechanism governing MB and PA production in W. cocos, highlighting the roles of squalene monooxygenase and glycoside hydrolases 16 carbohydrate-active enzymes.
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Letters in Applied Microbiology
Article . 2024 . Peer-reviewed
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Letters in Applied Microbiology
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Letters in Applied Microbiology
Article . 2024 . Peer-reviewed
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Data sources: Crossref
Letters in Applied Microbiology
Article . 2024
Data sources: Europe PubMed Central
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Optimizing liquid fermentation for Wolfiporia cocos: gene expression and biosynthesis of pachymic acid and mycelial biomass
descriptionPublicationkeyboard_double_arrow_right Article 2024Publisher:Oxford University Press (OUP)
Authors: Hamza Armghan, Noushahi; Aamir Hamid, Khan; Hamza Ali, Khan; Marcin, Kiedrzyński; +5 Authors
Hamza Armghan, Noushahi; Aamir Hamid, Khan; Hamza Ali, Khan; Marcin, Kiedrzyński; Adnan, Akbar; Raheel, Shahzad; Sri, Koerniati; Abdulwahed Fahad, Alrefaei; Shaohua, Shu;
doi: 10.1093/lambio/ovae054
pmid: 38866707
doi: 10.1093/lambio/ovae054
pmid: 38866707
Abstract Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine, holds immense potential for pharmaceutical and industrial applications. In this study, we aimed to optimize liquid fermentation techniques and culture medium composition to maximize mycelial biomass (MB) yield, pachymic acid (PA) concentration, and overall PA production. Additionally, we investigated the molecular basis of our findings by quantifying the expression levels of genes associated with PA and MB biosynthesis using quantitative real-time polymerase chain reaction. Under the optimized fermentation conditions, significant results were achieved, with maximum MB reaching 6.68 g l−1, PA content peaking at 1.25 mg g−1, and a total PA yield of 4.76 g l−1. Notably, among the four examined genes, squalene monooxygenase, exhibited enhanced expression at 0.06 ratio under the optimized conditions. Furthermore, within the realm of carbohydrate-active enzymes, the glycoside hydrolases 16 family displayed elevated expression levels at 21 ratios, particularly during MB production. This study enhances understanding of genetic mechanism governing MB and PA production in W. cocos, highlighting the roles of squalene monooxygenase and glycoside hydrolases 16 carbohydrate-active enzymes.
Letters in Applied M...arrow_drop_down
Letters in Applied Microbiology
Article . 2024 . Peer-reviewed
License: OUP Standard Publication Reuse
Data sources: Crossref
Letters in Applied Microbiology
Article . 2024
Data sources: Europe PubMed Central
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shareshareShare
citeCite
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Letters in Applied M...arrow_drop_down
Letters in Applied Microbiology
Article . 2024 . Peer-reviewed
License: OUP Standard Publication Reuse
Data sources: Crossref
Letters in Applied Microbiology
Article . 2024
Data sources: Europe PubMed Central
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
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