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This dataset contains terrestrial fluxes of nitrous oxide (N2O), methane (CH4) and ecosystem respiration (carbon dioxide (CO2)) calculated from static chamber measurements in riparian buffers of oil palm plantations on mineral soil, in Riau, Sumatra, Indonesia. Measurements were made monthly, from January 2019 until September 2021, with a break from April 2019 to October 2019 to allow for felling and replanting, and another break from January 2021 to June 2021 due to Covid-19 restrictions. To help to reduce the environmental impact of oil palm plantations, riparian buffers are now required by regulations in many Southeast Asian countries. The experiments were conducted to investigate the impact of greenhouse gas emissions from the riparian buffers. Research was funded through NERC grant NE/R000131/1 Sustainable Use of Natural Resources to Improve Human Health and Support Economic Development (SUNRISE) Greenhouse gas concentrations were measured using static chambers, enclosed for 45 minutes. Multiple regressions (including linear and hierarchical multiple regression) were fitted to calculate the best fit flux, using the RCflux R package, written by Dr Peter Levy (UKCEH).

Dynamics of societal material stocks such as buildings and infrastructures and their spatial patterns drive surging resource use and emissions. Building up and maintaining stocks requires large amounts of resources; currently stock-building materials amount to almost 60% of all materials used by humanity. Buildings, infrastructures and machinery shape social practices of production and consumption, thereby creating path dependencies for future resource use. They constitute the physical basis of the spatial organization of most socio-economic activities, for example as mobility networks, urbanization and settlement patterns and various other infrastructures. This dataset features a detailed map of material stocks for the whole of Germany on a 10m grid based on high resolution Earth Observation data (Sentinel-1 + Sentinel-2), crowd-sourced geodata (OSM) and material intensity factors. Temporal extent The map is representative for ca. 2018. Data format Per federal state, the data come in tiles of 30x30km (see shapefile). The projection is EPSG:3035. The images are compressed GeoTiff files (*.tif). There is a mosaic in GDAL Virtual format (*.vrt), which can readily be opened in most Geographic Information Systems. The dataset features area and mass for different street types area and mass for different rail types area and mass for other infrastructure area, volume and mass for different building types Masses are reported as total values, and per material category. Units area in m² height in m volume in m³ mass in t for infrastructure and buildings Further information For further information, please see the publication or contact Helmut Haberl (helmut.haberl@boku.ac.at). A web-visualization of this dataset is available here. Visit our website to learn more about our project MAT_STOCKS - Understanding the Role of Material Stock Patterns for the Transformation to a Sustainable Society. Publication Haberl, H., Wiedenhofer, D., Schug, F., Frantz, D., Virág, D., Plutzar, C., Gruhler, K., Lederer, J., Schiller, G. , Fishman, T., Lanau, M., Gattringer, A., Kemper, T., Liu, G., Tanikawa, H., van der Linden, S., Hostert, P. (accepted): High-resolution maps of material stocks in buildings and infrastructures in Austria and Germany. Environmental Science & Technology Funding This research was primarly funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (MAT_STOCKS, grant agreement No 741950). ML and GL acknowledge funding by the Independent Research Fund Denmark (CityWeight, 6111-00555B), ML thanks the Engineering and Physical Sciences Research Council (EPSRC; project Multi-Scale, Circular Economic Potential of Non-Residential Building Scale, EP/S029273/1), JL acknowledges funding by the Vienna Science and Technology Fund (WWTF), project ESR17-067, TF acknowledges the Israel Science Foundation grant no. 2706/19.

Implementing a circular economy aimed at reusing resources is becoming increasingly important for industry. Microalgae fit within a circular economy by being able to bioremediate nutrient waste and as a source of biomass for several commercial applications. Here, we report a novel validation of a circular economy concept using microalgae at a relevant industrial scale with a new two-phase process. During the first phase biomass was grown autotrophically, biomass was then concentrated using membrane technology for the second phase where mixotrophic conditions were applied to boost growth further. Microalgae cultures were able to grow (13.8 g/L), uptake and bioremediate nutrients (Nitrogen > 134 mg/L/day) from an anaerobic digestion side-stream (digestate), obtaining high quality microalgae biomass (>45% protein content) suitable for use as animal feed, closing the circular economy loop for industrial applications.

La production animale est une source essentielle de revenus et d'émissions de gaz à effet de serre (GES) agricoles en Colombie, au Brésil, en Argentine, au Costa Rica, en Uruguay, au Mexique et au Pérou. Plusieurs options de gestion et technologiques, avec un potentiel d'atténuation du méthane entérique, ont été évaluées et leur mise à l'échelle devrait contribuer à la réalisation des objectifs de réduction des émissions de GES. Pourtant, l'adoption généralisée d'options d'atténuation prometteuses reste limitée, ce qui soulève des questions quant à savoir si les objectifs de réduction des émissions envisagés sont réalisables. À l'aide de données générées localement, nous explorons les potentiels d'atténuation des technologies et des pratiques de gestion actuellement proposées pour atténuer les émissions de méthane entérique, pour les systèmes de production bovine dans les pays d'Amérique latine les plus émetteurs. Nous discutons ensuite des obstacles à l'adoption d'innovations qui réduisent considérablement les émissions de méthane entérique d'origine bovine et des changements majeurs dans les politiques et les pratiques qui sont nécessaires pour relever les ambitions nationales dans les pays à forte émission. En utilisant la science la plus récente et la pensée actuelle, nous fournissons notre point de vue sur une approche inclusive et ré-imaginons comment les secteurs universitaire, de la recherche, des affaires et des politiques publiques peuvent soutenir et encourager les changements nécessaires pour élever le niveau d'ambition et atteindre les objectifs de développement durable en envisageant des actions allant de la ferme à l'échelle nationale. La producción ganadera es una fuente fundamental de ingresos y emisiones de gases de efecto invernadero (GEI) agrícolas en Colombia, Brasil, Argentina, Costa Rica, Uruguay, México y Perú. Se han evaluado varias opciones de gestión y tecnológicas, con potencial de mitigación de metano entérico, y se prevé que su escalado contribuya al logro de los objetivos de reducción de emisiones de GEI. Sin embargo, la adopción generalizada de opciones de mitigación prometedoras sigue siendo limitada, lo que plantea dudas sobre si los objetivos de reducción de emisiones previstos son alcanzables. Utilizando datos generados localmente, exploramos los potenciales de mitigación de las tecnologías y prácticas de manejo actualmente propuestas para mitigar las emisiones de metano entérico, para los sistemas de producción ganadera en los países de mayor emisión de América Latina. Luego discutimos las barreras para adoptar innovaciones que reduzcan significativamente las emisiones de metano entérico en el ganado y los cambios importantes en las políticas y prácticas que se necesitan para aumentar las ambiciones nacionales en los países con altas emisiones. Utilizando la ciencia más reciente y el pensamiento actual, brindamos nuestra perspectiva sobre un enfoque inclusivo y reimaginamos cómo los sectores académico, de investigación, empresarial y de políticas públicas pueden apoyar e incentivar los cambios necesarios para elevar el nivel de ambición y alcanzar los objetivos de desarrollo sostenible considerando acciones desde la granja hasta la escala nacional. Livestock production is a pivotal source of income and agricultural greenhouse gas (GHG) emissions in Colombia, Brazil, Argentina, Costa Rica, Uruguay, Mexico and Peru. Several management and technological options, with enteric methane mitigation potential, have been evaluated and their scaling is anticipated to contribute towards achieving GHG emission reduction targets. Yet, widespread adoption of promising mitigation options remains limited, raising questions as to whether envisaged emission reduction targets are achievable. Using locally generated data, we explore the mitigation potentials of technologies and management practices currently proposed to mitigate enteric methane emissions, for cattle production systems in the higher emitting countries of Latin America. We then discuss barriers for adopting innovations that significantly reduce cattle-based enteric methane emissions and the major shifts in policy and practice that are needed to raise national ambitions in the high emitting countries. Using the latest science and current thinking, we provide our perspective on an inclusive approach and re-imagine how the academic, research, business and public policy sectors can support and incentivize the changes needed to raise the level of ambition and achieve sustainable development goals considering actions all the way from the farm to the national scale. الإنتاج الحيواني هو مصدر محوري للدخل وانبعاثات غازات الدفيئة الزراعية في كولومبيا والبرازيل والأرجنتين وكوستاريكا وأوروغواي والمكسيك وبيرو. تم تقييم العديد من خيارات الإدارة والخيارات التكنولوجية، مع إمكانية تخفيف الميثان المعوي، ومن المتوقع أن يساهم قياسها في تحقيق أهداف خفض انبعاثات غازات الدفيئة. ومع ذلك، لا يزال الاعتماد الواسع النطاق لخيارات التخفيف الواعدة محدودًا، مما يثير تساؤلات حول ما إذا كانت أهداف خفض الانبعاثات المتوخاة قابلة للتحقيق. باستخدام البيانات التي تم إنشاؤها محليًا، نستكشف إمكانات التخفيف من التقنيات وممارسات الإدارة المقترحة حاليًا للتخفيف من انبعاثات الميثان المعوية، لأنظمة إنتاج الماشية في البلدان ذات الانبعاثات الأعلى في أمريكا اللاتينية. ثم نناقش العوائق التي تحول دون اعتماد الابتكارات التي تقلل بشكل كبير من انبعاثات الميثان المعوي القائم على الماشية والتحولات الرئيسية في السياسات والممارسات اللازمة لرفع الطموحات الوطنية في البلدان ذات الانبعاثات العالية. باستخدام أحدث العلوم والتفكير الحالي، نقدم وجهة نظرنا حول نهج شامل ونعيد تصور كيف يمكن للقطاعات الأكاديمية والبحثية وقطاع الأعمال والسياسة العامة دعم وتحفيز التغييرات اللازمة لرفع مستوى الطموح وتحقيق أهداف التنمية المستدامة مع الأخذ في الاعتبار الإجراءات على طول الطريق من المزرعة إلى النطاق الوطني.

Significance Bioethanol produced from waste biomass from crops has the potential to provide a sustainable alternative to petroleum-based transportation fuel that does not compete with human food supply. The main obstacle to this approach is the resistance of this biomass to digestion. Thus, expensive energetic pretreatment and high enzyme inputs are needed to increase digestion. In this study, we screened a population of randomly mutated plants for digestibility with the aim of identifying novel factors that impact on this trait. We found a number of mutants with high digestibility and no impairments in growth or fitness. These mutants show a range of alterations in cell-wall composition, and we have mapped and characterized the mutant with the highest increase in digestibility.

Abstract Background Polylactic acid (PLA), a biodegradable polymer, has the potential to replace (at least partially) traditional petroleum-based plastics, minimizing “white pollution”. However, cost-effective production of optically pure L-lactic acid is needed to achieve the full potential of PLA. Currently, starch-based glucose is used for L-lactic acid fermentation by lactic acid bacteria. Due to its competition with food resources, an alternative non-food substrate such as cellulosic biomass is needed for L-lactic acid fermentation. Nevertheless, the substrate (sugar stream) derived from cellulosic biomass contains significant amounts of xylose, which is unfermentable by most lactic acid bacteria. However, the microorganisms that do ferment xylose usually carry out heterolactic acid fermentation. As a result, an alternative strain should be developed for homofermentative production of optically pure L-lactic acid using cellulosic biomass. Results In this study, an ethanologenic Escherichia coli strain, SZ470 (ΔfrdBC ΔldhA ΔackA ΔpflB ΔpdhR ::pflBp6-acEF-lpd ΔmgsA), was reengineered for homofermentative production of L-lactic acid from xylose (1.2 mole xylose = > 2 mole L-lactic acid), by deleting the alcohol dehydrogenase gene (adhE) and integrating the L-lactate dehydrogenase gene (ldhL) of Pediococcus acidilactici. The resulting strain, WL203, was metabolically evolved further through serial transfers in screw-cap tubes containing xylose, resulting in the strain WL204 with improved anaerobic cell growth. When tested in 70 g L-1 xylose fermentation (complex medium), WL204 produced 62 g L-1 L-lactic acid, with a maximum production rate of 1.631 g L-1 h-1 and a yield of 97% based on xylose metabolized. HPLC analysis using a chiral column showed that an L-lactic acid optical purity of 99.5% was achieved by WL204. Conclusions These results demonstrated that WL204 has the potential for homofermentative production of L-lactic acid using cellulosic biomass derived substrates, which contain a significant amount of xylose.

Although the importance of addressing ecosystem service benefits in regional land use planning and decision-making is evident, substantial practical challenges remain. In particular, methods to identify priority areas for the provision of key ecosystem services and other environmental services (benefits from the environment not directly linked to the function of ecosystems) need to be developed. Priority areas are locations which provide disproportionally high benefits from one or more service. Here we map a set of ecosystem and environmental services and delineate priority areas according to different scenarios. Each scenario is produced by a set of weightings allocated to different services and corresponds to different landscape management strategies which decision makers could undertake. Using the county of Cornwall, U.K., as a case study, we processed gridded maps of key ecosystem services and environmental services, including renewable energy production and urban development. We explored their spatial distribution patterns and their spatial covariance and spatial stationarity within the region. Finally we applied a complementarity-based priority ranking algorithm (zonation) using different weighting schemes. Our conclusions are that (i) there are two main patterns of service distribution in this region, clustered services (including agriculture, carbon stocks, urban development and plant production) and dispersed services (including cultural services, energy production and floods mitigation); (ii) more than half of the services are spatially correlated and there is high non-stationarity in the spatial covariance between services; and (iii) it is important to consider both ecosystem services and other environmental services in identifying priority areas. Different weighting schemes provoke drastic changes in the delineation of priority areas and therefore decision making processes need to carefully consider the relative values attributed to different services.