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The data consists of annual measurements of standing aboveground plant biomass, annual aboveground net primary productivity and annual soil respiration between 1998 and 2012. Data were collected from seven European shrublands that were subject to the climate manipulations drought and warming. Sites were located in the United Kingdom (UK), the Netherlands (NL), Denmark ( two sites, DK-B and DK-M), Hungary (HU), Spain (SP) and Italy (IT). All field sites consisted of untreated control plots, plots where the plant canopy air is artificially warmed during night time hours, and plots where rainfall is excluded from the plots at least during the plants growing season. Standing aboveground plant biomass (grams biomass per square metre) was measured in two undisturbed areas within the plots using the pin-point method (UK, DK-M, DK-B), or along a transect (IT, SP, HU, NL). Aboveground net primary productivity was calculated from measurements of standing aboveground plant biomass estimates and litterfall measurements. Soil respiration was measured in pre-installed opaque soil collars bi-weekly, monthly, or in measurement campaigns (SP only). The datasets provided are the basis for the data analysis presented in Reinsch et al. (2017) Shrubland primary production and soil respiration diverge along European climate gradient. Scientific Reports 7:43952 https://doi.org/10.1038/srep43952 Standing biomass was measured using the non-destructive pin-point method to assess aboveground biomass. Measurements were conducted at the state of peak biomass specific for each site. Litterfall was measured annually using litterfall traps. Litter collected in the traps was dried and the weight was measured. Aboveground biomass productivity was estimated as the difference between the measured standing biomass in year x minus the standing biomass measured the previous year. Soil respiration was measured bi-weekly or monthly, or in campaigns (Spain only). It was measured on permanently installed soil collars in treatment plots. The Gaussen Index of Aridity (an index that combines information on rainfall and temperature) was calculated using mean annual precipitation, mean annual temperature. The reduction in precipitation and increase in temperature for each site was used to calculate the Gaussen Index for the climate treatments for each site. Data of standing biomass and soil respiration was provided by the site responsible. Data from all sites were collated into one data file for data analysis. A summary data set was combined with information on the Gaussen Index of Aridity Data were then exported from these Excel spreadsheet to .csv files for ingestion into the EIDC.

Although GPS coordinates for current populations are not included due to the potential threat of poaching, the climate variables for each species are provided. The records for extant gecko and skinks mainly came from the New Zealand's Department of Conervation Herpetofauna Database. After updating the taxonomy and cleaning the data to reflect the taxonomy as at 2019 of 43 geckos speceis recognised across seven genera and 61 species in genus, we then thinned the occurrence records at a 1 km resolution for all species then predicted distributions for those with > 15 records using species distribution models. The climate variables for each species were selected among annual mean temperature (bio1), maximum temperature of the warmest month (bio5), minimum temperature of the coldest month (bio6), mean temperature of driest quarter (bio9), mean temperature of wettest quarter (bio10), and precipitation of the driest quarter (bio17). To reduce multicollinearity in species distribution models for each species, we only retained climate variables with a variable inflation factor < 10. The climate variables were from the CHELSA database (https://chelsa-climate.org/), which can be freely downloaded for current and future scenarios. We also provide MCC tree files for the geckos and skinks. The phylogenetic trees have been constructed for NZ geckos by (Nielsen et al., 2011) and for NZ skinks by (Chapple et al., 2009). For geckos we used a subset of the sequences used by Nielsen et al. (2011) for four genes, two nuclear (RAG 1, PDC) and two mitochondrial (16S, ND2 along with flanking tRNA sequences). For skinks, we used sequences from Chapple et al. (2009) for one nuclear (RAG 1) and five mitochondrial (ND2, ND4, Cyt b, 12S and 16S) genes, and additional ND2 sequences for taxa not included in the original phylogeny (Chapple et al., 2011, p. 201). In total we used sequences for all recognised extant taxa (Hitchmough et al., 2016) as at 2019 except for three species of skink (O. aff. inconspicuum “Okuru”, O. robinsoni, and O. aff. inconspicuum “North Otago”) and two species of gecko (M. “Cupola” and W. “Kaikouras”) for which genetic data were not available. Aim: The primary drivers of species and population extirpations have been habitat loss, overexploitation, and invasive species, but human-mediated climate change is expected to be a major driver in future. To minimise biodiversity loss, conservation managers should identify species vulnerable to climate change and prioritise their protection. Here, we estimate climatic suitability for two speciose taxonomic groups, then use phylogenetic analyses to assess vulnerability to climate change. Location: Aotearoa New Zealand (NZ) Taxa: NZ lizards: diplodactylid geckos and eugongylinae skinks Methods: We built correlative species distribution models (SDMs) for NZ geckos and skinks to estimate climatic suitability under current climate and 2070 future-climate scenarios. We then used Bayesian phylogenetic mixed models (BPMMs) to assess vulnerability for both groups with predictor variables for life history traits (body size and activity phase) and current distribution (elevation and latitude). We explored two scenarios: an unlimited dispersal scenario, where projections track climate, and a no-dispersal scenario, where projections are restricted to areas currently identified as suitable. Results: SDMs projected vulnerability to climate change for most modelled lizards. For species’ ranges projected to decline in climatically suitable areas, average decreases were between 42–45% for geckos and 33–91% for skinks, although area did increase or remain stable for a minority of species. For the no-dispersal scenario, the average decrease for geckos was 37–52% and for skinks was 33–52%. Our BPMMs showed phylogenetic signal in climate change vulnerability for both groups, with elevation increasing vulnerability for geckos, and body size reducing vulnerability for skinks. Main conclusions: NZ lizards showed variable vulnerability to climate change, with most species’ ranges predicted to decrease. For species whose suitable climatic space is projected to disappear from within their current range, managed relocation could be considered to establish populations in regions that will be suitable under future climates.

Journal of Economic Inequality, accepted

This dataset and codebook correspond to the second round of survey data gathered in Denmark in 2023, within the project FULFILL - Fundamental Decarbonisation Through Sufficiency By Lifestyle Changes. As part of Work Package 3 (WP3) in the FULFILL project, we collected quantitative data from six countries: Denmark, France, Germany, Italy, Latvia, and India. The first round of the survey, consisted of recruiting a representative sample of approximately 2000 households in each country. In this second survey round, we recruit around 500 respondents from the initial survey round, ensuring representativity is maintained. This survey is very similar to the survey in the first round and includes a lot of identical items, including a quantitative assessment of the carbon footprint in the housing, mobility, and diet sectors, socio-economic factors such as age, gender, income, education, household size, life stage, and political orientation. Furthermore, the survey includes measures of quality of life, encompassing aspects such as health and well-being, environmental quality, financial security, and comfort. New for this second round, we have incorporated questions regarding the measures respondents adopted in response to the 2022 energy crisis.

Fashion products made from repurposed materials (e.g., backpacks made from pineapple leaves) have become more prevalent nowadays, and their environmental sustainability is one of the core advantages. Yet, it is currently unclear how consumers respond to products made from repurposed materials. We conducted three experiments to examine the effects of three material features, namely function, sustainability, and distinguishability, on consumer preferences for fashion products made from repurposed materials. The results indicate that, when the function of repurposed materials is as good as that of conventional materials, consumers prefer a product made from repurposed materials over the same product made from conventional materials. Also, consumers in general prefer repurposed materials to be less visually distinguishable. Finally, when the sustainability of the repurposed products is emphasized, consumers appear more likely to choose products made from repurposed materials, even when these products have an inferior function. In conclusion, to promote fashion products made from repurposed materials, marketers may emphasize the function and sustainability of repurposed materials, and producers may manufacture repurposed materials that visually resemble conventional materials.

This database includes more than 100 decarbonisation innovations in Paper, Plastic, Steel and Meat & Dairy sectors, across their value chains, as well as in Finance. For each innovation there is a description, information about its contribution to decarbonisation, actors and collaborators involved, sources of funding, drivers, (co)benefits and disadvantages. More information on the method for selecting innovations for the database is available here. The database was created as part of REINVENT – a Horizon 2020 research project funded by the European Commission (grant agreement 730053). REINVENT involves five research institutions from four countries: Lund University (Sweden), Durham University (United Kingdom), Wuppertal Institute (Germany), PBL Netherlands Environmental Assessment Agency (the Netherlands) and Utrecht University (the Netherlands). More information can be found on our website: www.reinvent-project.eu.

The atmosphere concentration of CO2 is steadily increasing and causing climate change. To achieve the Paris 1.5 or 2 oC target, negative emissions technologies must be deployed in addition to reducing carbon emissions. The ocean is a large carbon sink but the potential of marine primary producers to contribute to carbon neutrality remains unclear. Here we review the alterations to carbon capture and sequestration of marine primary producers (including traditional ‘blue carbon’ plants, microalgae, and macroalgae) in the Anthropocene, and, for the first time, assess and compare the potential of various marine primary producers to carbon neutrality and climate change mitigation via biogeoengineering approaches. The contributions of marine primary producers to carbon sequestration have been decreasing in the Anthropocene due to the decrease in biomass driven by direct anthropogenic activities and climate change. The potential of blue carbon plants (mangroves, saltmarshes, and seagrasses) is limited by the available areas for their revegetation. Microalgae appear to have a large potential due to their ubiquity but how to enhance their carbon sequestration efficiency is very complex and uncertain. On the other hand, macroalgae can play an essential role in mitigating climate change through extensive offshore cultivation due to higher carbon sequestration capacity and substantial available areas. This approach seems both technically and economically feasible due to the development of offshore aquaculture and a well-established market for macroalgal products. Synthesis and applications: This paper provides new insights and suggests promising directions for utilizing marine primary producers to achieve the Paris temperature target. We propose that macroalgae cultivation can play an essential role in attaining carbon neutrality and climate change mitigation, although its ecological impacts need to be assessed further. To calculate the parameters presented in Table 1, the relevant keywords "mangroves, salt marshes, macroalgae, microalgae, global area, net primary productivity, CO2 sequestration" were searched through the ISI Web of Science and Google Scholar in July 2021. Recent data published after 2010 were collected and used since area and productivity of plants change with decade. For data with limited availability, such as net primary productivity (NPP) of seagrasses and global area and NPP of wild macroalgae, data collection was extended back to 1980. Total NPP and CO2 sequestration for mangroves, salt marshes, seagrasses and wild macroalgae were obtained by the multiplication of area and NPP/CO2 sequestration density and subjected to error propagation analysis. Data were expressed as means ± standard error.

# Data from: Eocene Shark Teeth from Peninsular Antarctica: Windows to Habitat Use and Paleoceanography. [https://doi.org/10.5061/dryad.qz612jmq2](https://doi.org/10.5061/dryad.qz612jmq2) The repository folder includes scripts and spreadsheets for phosphate oxygen stable isotope (δ18Op) analysis measured from shark tooth biogenic apatite collected from the Eocene deposits of the La Meseta and Submeseta formations (West Antarctica, Seymour Island). It also contains Fourier-Transform Infrared Spectroscopy (FTIR) analysis, a Bayesian model for temperature estimates, and model output extraction scripts from the iCESM simulation for the Early Eocene (Zhu et al., 2020). Scripts and data are stored in specific folders on the type of analysis. All scripts are in R or Python language. **Usage notes** **1 "iCESM modeling scripts" directory** The folder includes scripts in Jupiter Notebook format for extracting and plotting iCESM seawater outputs for the Eocene. The folder includes two files: 1) “d18Ow Analysis Script.ipynb” - This is a Python script primarily using the XArray library, to import iCESM output from Zhu et al. (2020), calculating δ18Ow, and reorganizing the output into monthly time intervals along 25 m and 115 m depth slices, while also averaging output down to these depths; 2) “NetCDF Plotting.ipynb” - this is a Python script primarily using the XArray, Matplotlib, and Cartopy libraries. The script writes a single callable function that creates Matplotlib contour plots from iCESM history output. Variables include temperature, salinity, ideal age, oxygen isotopes, and neodymium isotopes, and map projections include Plate Carree, Mollweide, and orthographic (centering on the Drake Passage). Options are built to enable scale normalization or to set maximum and minimum values for data and select colormaps from a predefined selection of Matplotlib’s “Spectral”, “Viridis”, “Coolwarm”, “GNUplot2”, “PiYG”, “RdYlBu”, and “RdYlGn”. For further questions on model output scripts, please email Adam Aleksinski at [aaleksin@purdue.edu](https://datadryad.org/stash/dataset/doi:10.5061/aaleksin@purdue.edu). **2 "d18O data and maps" directory** The folder includes δ18Op of shark tooth bioapatite and other datasets to interpret shark paleoecology. These datasets include: · δ18Op of shark tooth bioapatite (“shark FEST d18Op.csv”). Isotope measurements were run at the Stable Isotope Ecosystem Laboratory of (SIELO) University of California, Merced (California, USA). · Reference silver phosphate material δ18Op for analytical accuracy and precision (“TCEA reference materials.csv"). Isotope measurements were run at the Stable Isotope Ecosystem Laboratory of (SIELO) University of California, Merced (California, USA). · Bulk and serially sampled δ18Oc data of co-occurring bivalves (Ivany et al., 2008; Judd et al., 2019) (“Ivany et al. 2008_bulk.csv” and “Judd et al., 2019_serial sampling.csv"). · iCESM model temperature and δ18Ow outputs at 3x and 6x pre-industrial CO2 levels for the Early Eocene (Zhu et al., 2020) (“SpinupX3_25m_Mean_Monthly.nc”, “SpinupX6_25m_Mean_Monthly.nc.”, and “CA_x3CO2.csv”). Simulations are integrated from the surface to 25 m. · δ18O values of invertebrate species published in Longinelli (1965) and Longinelli & Nuti (1973), used to convert bulk δ18Oc (V-SMOW) data of bivalves into δ18Op (V-SMOW) values after δ18Oc (V-PDB) - δ18Oc (V-SMOW) conversion found in Kim et al. (2015) (“d18O carbonate and phosphate references.csv”). · R script for data analysis ("d18O data and maps.Rmd”). The script provides annotation through libraries, instrumental accuracy and precision tests, tables, statistical analysis, figures, and model output extractions. . ("TELM_diversity.csv") displays diversity trends of chondrichthyans across TELMs in one of the main figures of the manuscript. **2.1 Dataset description** **shark FEST d18Op.csv** · *Sample_ID*: Identification number of tooth specimens. · *Other_ID*: Temporary identification number of tooth specimens. · *Taxon*: Species assigned to shark tooth specimens. · *TELM*: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013). · *d18Op*: Mean δ18Op values of silver phosphate crystals precipitated from shark tooth bioapatite. Specimens were run in triplicates, corrected, and standardized on the V-SMOW scale. · *sd*: Standard deviation of silver phosphate triplicate samples per specimen. · *Protocol*: Silver phosphate protocols used to precipitate crystals from shark tooth bioapatite. We adopted the Rapid UC (“UC_Rapid”) and the SPORA (“SPORA”) protocols after Mine et al. and (2017) Larocca Conte et al. (2024) based on the tooth specimen size and sampling strategy. Descriptions of the methods are included in the main manuscript. · *Environment*: Inferred shark habitat based on taxonomy classified as benthic or pelagic environment. · *Collection*: Institutional abbreviations of museum collections from which shark tooth specimens are housed. NRM-PZ is the abbreviation for the Swedish Natural History Museum (Stockholm, Sweden), PRI is the abbreviation for the Paleontological Research Institute (Ithaca, New York, United States), and UCMP is the University of California Museum of Paleontology (Berkeley, California, United States). **TCEA reference materials.csv** · *Identifier_1*: unique identifier number per sample. · *sample*: reference silver phosphate materials (USGS 80 and USGS 81). · *amount*: weight of samples in mg. · *Area 28*: peak area of mass 28 (12C16O). · *Area 30*: peak area of mass 30 (12C18O). · *d18O_corrected*: corrected δ18Op value of reference materials following drift correction, linearity correction, and 2-point calibration to report values on the V-SMOW scale. **Ivany et al. 2008_bulk.csv** · *Telm*: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013). · *Locality*: Locality code from which bivalves were collected. · *Genus*: Genera of bivalves. Specimens are assigned to *Cucullaea* and *Eurhomalea* genera. · *Line*: Sampling areas of specimens. The sampling strategy is described in Ivany et al. (2008). · *d13C*: δ13C values of specimens from sampled lines. Values are reported in the V-PDB scale. · *d18Oc_PDB*: δ18Oc values of specimens from sampled lines. Values are reported in the V-PDB scale. **Judd et al., 2019_serial sampling.csv** · *Horizon:* horizons of the TELM 5 unit (La Meseta Formation) from which bivalves were collected. Horizon 1 is stratigraphically the lowest, while horizon 4 is the highest (Judd et al., 2019). · *ID*: Identification number of specimens. · *Latitude*: Geographic coordinate where bivalve specimens were collected. · *Longitude*: Geographic coordinate where bivalve specimens were collected. · *Surface sampled*: Specific sampling area, indicating whether sampling occurred in the interior or exterior portion of shells. · *distance*: The distance from the umbo in mm from which sampling occurred along a single shell. · *d18Oc_PDB*: δ18Oc values of specimens from sampled areas of shells. Values are reported on the V-PDB scale. **SpinupX3_25m_Mean_Monthly.nc** See section 1 ("iCESM modeling scripts" directory, “d18Ow Analysis Script.ipynb” script) for a full description of the iCESM model output extraction. **SpinupX6_25m_Mean_Monthly.nc** See section 1 ("iCESM modeling scripts" directory, “d18Ow Analysis Script.ipynb” script) for a full description of the iCESM model output extraction. **CA_x3CO2.csv** · *lat*: Geographic coordinate where temperature and δ18Ow model values are extracted from the iCESM simulation scaled at 3x preindustrial CO2 levels (values averaged within a seawater column depth of 25 m). · *long*: Geographic coordinate where temperature and δ18Ow model values are extracted from the iCESM simulation scaled at 3x preindustrial CO2 levels (values averaged within a seawater column depth of 25 m). · *T_mean*: Simulated seawater temperature values in °C. · *d18Ow*: Simulated seawater δ18Ow values (V-SMOW). · *d18Op*: Simulated seawater δ18Op values (V-SMOW). Values were calculated by using seawater temperature and δ18Ow arrays following the paleothermometer equation after Lécuyer et al. (2013). **d18O carbonate and phosphate references.csv** · *species*: Species of invertebrate taxa. · *type*: Specimen type, including barnacles, brachiopods, crabs, and mollusks. · *depth*: Depth of seawater column where specimens were collected, reported in meters below sea level when specified. · *d18Op*: δ18Op values of invertebrate specimens (V-SMOW). · *d18Oc_PDB*: δ18Oc values of invertebrate specimens (V-PDB). · *Reference*: Citations from which data were taken to build the dataset (Longinelli, 1965; Longinelli & Nuti, 1973). **TELM diversity.csv** · *genus:* genera of sharks and rays compiled from literature (Engelbrecht et al., 2016a, 2016b, 2017a, 2017b, 2019; Kriwet, 2005; Kriwet et al., 2016; Long, 1992; Marramá et al., 2018). · *species*: species of sharks and rays compiled from literature (Engelbrecht et al., 2016a, 2016b, 2017a, 2017b, 2019; Kriwet, 2005; Kriwet et al., 2016; Long, 1992; Marramá et al., 2018). · *Environment*: Inferred shark habitat based on taxonomy classified as benthic or pelagic environment. · *TELM*: Stratigraphic units of La Meseta (TELM 1-5; ~44 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013). **3 “FTIR data” directory** The folder includes FTIR acquisitions and data analysis scripts on reference materials and shark tooth bioapatite for quality checks to test diagenesis effects on δ18Op of sharks. The folder includes: · The R project file “apatite_ftir.Rproj”. This project file navigates through scripts for raw data processing and data analysis. The background of the raw data was processed following custom R functions from Trayler et al. (2023; [https://github.com/robintrayler/collagen_demineralization](https://github.com/robintrayler/collagen_demineralization)). · The “.Rproj.user” folder includes project-specific temporary files (e.g. auto-saved source documents, window-state, etc.) stored by the R project file “apatite_ftir.Rproj”. The folder may be hidden depending on directory view options. · The “raw data” directory stores spectra acquisitions as .dpt files. Spectra files are stored in the folders “apatite” and “calcite” based on the material type. Spectra were obtained in the 400 – 4000 cm⁻¹ range using a Bruker Vertex 70 Far-Infrared in ATR located at the Nuclear Magnetic Resonance Facility at the University of California Merced (California, USA). · The “processed” directory includes processed spectra stored as .csv files (“apatite_data.csv” and “calcite_data.csv”) following the background correction (Trayler et al., 2023) and processed infrared data from Larocca Conte et al. (2024) (“Larocca Conte et al._SPORA_apatite_data.csv”) from which the NIST SRM 120c spectrum was filtered. Infrared spectra data in “Larocca Conte et al._SPORA_apatite_data.csv” were obtained and corrected following the same methodologies mentioned above. · The “R” directory includes R scripts of customized source functions for background correction (Trayler et al., 2023; inspect the "functions" directory and the R script "0_process_data.R") and data analysis (“data_analysis.R”). The scripts provide annotation through libraries and functions used for data processing and analysis. · Additional datasets. The “data_FTIR_d18O.csv” includes infrared data and δ18Op values of specimens, while the “Grunenwald et al., 2014_CO3.csv” is the dataset after Grunenwald et al. (2014) used to predict carbonate content from the materials featured in this work. **3.1 Dataset description** Spreadsheets included in the “processed” directory The datasets “apatite_data.csv”, “calcite_data.csv”, and “Larocca Conte et al._SPORA_apatite_data.csv” are structured with the following variables: · *wavenumber*: infrared wavenumber in cm-1. · *absorbance*: infrared absorbance value. · *file_name:* .dpt file name from which infrared wavenumber and absorbance values were obtained following the background correction. **data_FTIR_d18O.csv** · *file_name:* .dpt file name from which infrared wavenumber and absorbance values were obtained following the background correction. · *v4PO4_565_wavenumber*: Wavenumber of maximum infrared absorbance around the first νPO4 band, usually at 565 cm-1. · *v4PO4_565*: Peak absorbance value of the first ν4PO4 band (~565 cm-1). · *v4PO4_valley_wavenumber*: Wavenumber of valley between ν4PO4 bands. · *v4PO4_valley*: Absorbance value of the valley between ν4PO4 bands. · *v4PO4_603_wavenumber*: Wavenumber of maximum infrared absorbance around the second ν4PO4 band, usually at 603 cm-1. · *v4PO4_603*: Peak absorbance value of the second ν4PO4 band (~603 cm-1). · *CI*: Crystallinity index calculated after equation provided in (Shemesh, 1990) as (*v4PO4_565* + *v4PO4_603* / *v4PO4_valley*) (i.e., the sum of peak absorbance of νPO4 bands divided by the absorbance value of the valley between peaks). · *material*: Material type of samples (i.e., standard material, enameloid, dentin sampled from the crown or root area of shark teeth, and enameloid mixed with dentin). · *AUC_v3PO4*: Area under the curve of the ν3PO4 and ν1PO4 bands where maximum absorbance is at ~1025 cm-1 and ~960 cm-1, respectively. · *AUC_v3CO3*: Area under the curves of Type-A and Type-B carbonate bands having maximum infrared absorbance at ~1410 (Type-B), ~1456 (Type-B), and ~1545 cm-1 (Type-A). · *v3CO3_v3PO4_ratio*: Ratio between area under the curves of carbonate and phosphate bands (i.e., *AUC_v3CO3* / *AUC_v3PO4*). · *CO3_wt*: Estimated mean carbonate content following the equation in Grunenwald et al. (2014) (i.e. *CO3_wt* = 28.4793 (±1.4803) *v3CO3_v3PO4_ratio* + 0.1808(±0.2710); R2 = 0.985). · *CO3_wt_sd*: Standard deviation of estimated carbonate content calculated by propagating the error around coefficients provided in the Grunenwald et al. (2014) equation (see full equation in *CO3_wt*). · *Taxon*: Species assigned to shark tooth specimens. · *TELM*: Stratigraphic units of La Meseta (TELM 2-5; ~45 to ~37 Ma) and Submeseta formations (TELMs 6 and 7; ~37 to ~34 Ma) (Amenábar et al., 2020; Douglas et al., 2014; Montes et al., 2013). · *d18Op*: Mean δ18Op values of silver phosphate crystals precipitated from shark tooth bioapatite. Specimens were run in triplicates, corrected, and standardized on the V-SMOW scale. · *sd*: Standard deviation of silver phosphate triplicate samples per specimen. · *Collection*: Institutional abbreviations of museum collections where shark tooth specimens are housed. Infrared spectra were obtained from a selected subset of tooth specimens in the care of the Swedish Natural History Museum (NRM-PZ; Stockholm, Sweden). **Grunenwald et al., 2014_CO3.csv** · *sample*: Sample code. · *material*: Material type of samples (i.e., standard material, bone, and enamel). · *v3CO3*: Area under the curves of Type-A and Type-B carbonate bands having maximum infrared absorbance at ~1410 (Type-B), ~1456 (Type-B), and ~1545 cm-1 (Type-A). · *v3PO4*: *AUC_v3PO4*: Area under the curve of the ν3PO4 and ν1PO4 bands where maximum absorbance is at ~1025 cm-1 and ~960 cm-1, respectively. · *v3CO3_v3PO4_ratio*: *v3CO3_v3PO4_ratio*: Ratio between area under the curves of carbonate and phosphate bands (i.e., *v3CO3* /*v3PO4*). · *CO3_wt*: Carbonate content measured via CO2 coulometry. Further details about the analytical measurements are found in Grunenwald et al. (2014). **4 “Bayes_FEST_Temperautre Estimates” directory** The folder includes the Bayesian approach used to estimate posterior seawater temperature, δ18Ow values from δ18Op of sharks bioapatite using a Bayesian approach modified after Griffiths et al. (2023). The original scripts used in Griffiths et al. (2023) are reposited here: [https://github.com/robintrayler/bayesian_phosphate](https://github.com/robintrayler/bayesian_phosphate). The directory includes: · The R project file “Bayes_FEST.Rproj”. This project file navigates through scripts for raw data analysis. · The “.Rproj.user” folder includes project-specific temporary files (e.g. auto-saved source documents, window-state, etc.) stored by the R project file “Bayes_FEST.Rproj”. The folder may be hidden depending on directory view options. · The “data” folder includes the spreadsheets for modeled seawater temperature and δ18Ow values (“CA_x3CO2.csv”) and δ18Op values of shark tooth bioapatite (“shark FEST d18Op.csv”) used as prior information for the Bayesian model. We refer to section 2.1 for the full description of spreadsheets. · The “R” folder includes customized functions for the Bayesian model stored in the “functions” directory and the script for data analysis (“01_model_sharks.R”). The script includes a comparison of paleothermometer equations after Kolodny et al. (1983), Lécuyer et al. (2013), Longinelli & Nuti (1973), and (Pucéat et al. (2010) using the bulk δ18Op shark tooth bioapatite, simulated seawater temperature and δ18Ow values as prior inputs. While all paleothermometers estimate similar posterior bulk δ18Op close to empirical values, temperature estimates using the Pucéat et al. (2010) method are often the highest, generating estimates ~8°C higher than other equations. We therefore used the Lécuyer et al. (2013) paleothermomether for temperature estimates using δ18Op of shark bioapatite grouped by taxa because it: 1\) Provides consistent posterior temperature estimates relative to other equations (Longinelli & Nuti, 1973, Kolodny et al., 1983). 2\) provides temperature values from fish tooth specimens consistent with estimates of co-existing bivalves or brachiopod carbonate shells. The script provides annotation through libraries, statistical analysis, figures, and tables. **4 Software** **4.1 R** R and R Studio (R Development Core Team, 2024; RStudio Team, 2024) are required to run scripts included in the "d18O data and maps", “FTIR data”, and “Bayes_FEST_Temperautre Estimates” directories, which were created using versions 4.4.1 and 2024.04.02, respectively. Install the following libraries before running scripts: “cowplot” (Wilke, 2024), “colorspace” (Zeileis et al., 2020), “DescTools” (Signorell, 2024), “lattice” (Sarkar, 2008), “flextable” (Gohel & Skintzos, 2024), “ggh4x” (van den Brand, 2024), “ggnewscale” (Campitelli, 2024), “ggpubr” (Kassambara, 2023a), “ggspatial” (Dunnington, 2023), “ggstance” (Henry et al., 2024), “ggstar” (Xu, 2022), “greekLetters” (Kévin Allan Sales Rodrigues, 2023), “gridExtra” (Auguie, 2017), “mapdata” (code by Richard A. Becker & version by Ray Brownrigg., 2022); “mapproj” (for R by Ray Brownrigg et al., 2023), “maps” (code by Richard A. Becker et al., 2023), “ncdf4” (Pierce, 2023), “oce” (Kelley & Richards, 2023), “rasterVis” (Oscar Perpiñán & Robert Hijmans, 2023), “RColorBrewer” (Neuwirth, 2022), “rnaturalearth” (Massicotte & South, 2023), “rnaturalearthhires” (South et al., 2024),”rstatix” (Kassambara, 2023b), “scales” (Wickham et al., 2023), “tidyverse” (Wickham et al., 2019), “viridisLite” (Garnier et al., 2023). **4.2 Python** Python scripts, including “d18O Analysis Script.ipynb” and “NetCDF Plotting.ipynb”, utilize the Jupyter Notebook interactive ‘platform and are executed using Python version 3.9.16. Install the following libraries before running scripts: “xarray” (Hoyer & Joseph, 2017), “matplotlib” (Hunter, 2007), “cartopy” (Met Office, 2015). **5 References** Amenábar, C. R., Montes, M., Nozal, F., & Santillana, S. (2020). 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(2022). ggstar: Multiple Geometric Shape Point Layer for “ggplot2.” Retrieved from [https://cran.r-project.org/package=ggstar](https://cran.r-project.org/package=ggstar) Zeileis, A., Fisher, J. C., Hornik, K., Ihaka, R., McWhite, C. D., Murrell, P., et al. (2020). {colorspace}: A Toolbox for Manipulating and Assessing Colors and Palettes. *Journal of Statistical Software*, *96*(1), 1–49. [https://doi.org/10.18637/jss.v096.i01](https://doi.org/10.18637/jss.v096.i01) Zhu, J., Poulsen, C. J., Otto-Bliesner, B. L., Liu, Z., Brady, E. C., & Noone, D. C. (2020). Simulation of early Eocene water isotopes using an Earth system model and its implication for past climate reconstruction. *Earth and Planetary Science Letters*, *537*, 116164. [https://doi.org/10.1016/j.epsl.2020.116164](https://doi.org/10.1016/j.epsl.2020.116164) Eocene climate cooling, driven by the falling pCO2 and tectonic changes in the Southern Ocean, impacted marine ecosystems. Sharks in high-latitude oceans, sensitive to these changes, offer insights into both environmental shifts and biological responses, yet few paleoecological studies exist. The Middle-to-Late Eocene units on Seymour Island, Antarctica, provide a rich, diverse fossil record, including sharks. We analyzed the oxygen isotope composition of phosphate from shark tooth bioapatite (δ18Op) and compared our results to co-occurring bivalves and predictions from an isotope-enabled global climate model to investigate habitat use and environmental conditions. Bulk δ18Op values (mean 22.0 ± 1.3‰) show no significant changes through the Eocene. Furthermore, the variation in bulk δ18Op values often exceeds that in simulated seasonal and regional values. Pelagic and benthic sharks exhibit similar δ18Op values across units but are offset relative to bivalve and modeled values. Some taxa suggest movements into warmer or more brackish waters (e.g., Striatolamia, Carcharias) or deeper, colder waters (e.g., Pristiophorus). Taxa like Raja and Squalus display no shift, tracking local conditions in Seymour Island. The lack of difference in δ18Op values between pelagic and benthic sharks in the Late Eocene could suggest a poorly stratified water column, inconsistent with a fully opened Drake Passage. Our findings demonstrate that shark tooth bioapatite tracks the preferred habitat conditions for individual taxa rather than recording environmental conditions where they are found. A lack of secular variation in δ18Op values says more about species ecology than the absence of regional or global environmental changes. See methods in Larocca Conte, G., Aleksinski, A., Liao, A., Kriwet, J., Mörs, T., Trayler, R. B., Ivany, L. C., Huber, M., Kim, S. L. (2024). Eocene Shark Teeth From Peninsular Antarctica: Windows to Habitat Use and Paleoceanography. Paleoceanography and Paleoclimatology, 39, e2024PA004965.