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Clostridium kluyveri is an anaerobic microorganism that is well-known for producing butyrate and hexanoate using ethanol and acetate. It is also an important bacterium in the production of Chinese strong flavour baijiu (SFB). To obtain a comprehensive understanding of its metabolism, a curated genome-scale metabolic model (GSMM) of C. kluyveri, including 708 genes, 994 reactions, and 804 metabolites, was constructed and named iCKL708. This model was used to simulate the growth of C. kluyveri on different carbon substrates and the results agreed well with the experimental data. The butyrate, pentanoate, and hexanoate biosynthesis pathways were also elucidated. Flux balance analysis indicated that the ratio of ethanol to acetate, as well as the uptake rate of carbon dioxide, affected hexanoate production. The GSMM iCKL708 described here provides a platform to further our understanding and exploration of the metabolic potential of C. kluyveri.

Clostridium kluyveri is an anaerobic microorganism that is well-known for producing butyrate and hexanoate using ethanol and acetate. It is also an important bacterium in the production of Chinese strong flavour baijiu (SFB). To obtain a comprehensive understanding of its metabolism, a curated genome-scale metabolic model (GSMM) of C. kluyveri, including 708 genes, 994 reactions, and 804 metabolites, was constructed and named iCKL708. This model was used to simulate the growth of C. kluyveri on different carbon substrates and the results agreed well with the experimental data. The butyrate, pentanoate, and hexanoate biosynthesis pathways were also elucidated. Flux balance analysis indicated that the ratio of ethanol to acetate, as well as the uptake rate of carbon dioxide, affected hexanoate production. The GSMM iCKL708 described here provides a platform to further our understanding and exploration of the metabolic potential of C. kluyveri.

Abstract The accurate information of the thermal stresses and temperature in isotropic elastic solids is the key for many engineering applications. At present the classical linear coupled theory of thermoelasticity deduced with the assumptions of small temperature changes is widely used to solve the thermoelastic problems in engineering. In this paper, to describe the thermoelastic behavior in isotropic solids undergoing large temperature changes more accurately, the novel coupled models of thermoelasticity and the corresponding finite element models have been presented explicitly and validated by experimental measurement. The effect of large temperature changes on the solutions of thermoelastic problems is discussed. For the heat transfer process, if the isotropic elastic solids will expand when heated and contract when cooled and the condition d E E d T · σ i j E − δ i j 1 − 2 ν α 0 can be met in the context of small deformations, the effect of large temperature changes can be regarded as increasing the specific heat. The proposed models are applied to solve two thermoelastic problems. From the obtained numerical results, the effect of large temperature changes will increase with the amplitude of temperature change and may be considerably even when the temperature changes slowly.

Abstract The accurate information of the thermal stresses and temperature in isotropic elastic solids is the key for many engineering applications. At present the classical linear coupled theory of thermoelasticity deduced with the assumptions of small temperature changes is widely used to solve the thermoelastic problems in engineering. In this paper, to describe the thermoelastic behavior in isotropic solids undergoing large temperature changes more accurately, the novel coupled models of thermoelasticity and the corresponding finite element models have been presented explicitly and validated by experimental measurement. The effect of large temperature changes on the solutions of thermoelastic problems is discussed. For the heat transfer process, if the isotropic elastic solids will expand when heated and contract when cooled and the condition d E E d T · σ i j E − δ i j 1 − 2 ν α 0 can be met in the context of small deformations, the effect of large temperature changes can be regarded as increasing the specific heat. The proposed models are applied to solve two thermoelastic problems. From the obtained numerical results, the effect of large temperature changes will increase with the amplitude of temperature change and may be considerably even when the temperature changes slowly.

Abstract The reverse flat-plate collector is a non-concentrating collector. It can collect solar heat at high temperatures which cannot be achieved by conventional non-concentrating collectors. In this paper, the authors have proposed a number of modified versions of the originally proposed reverse flat-plate collector. The new designs are of single, as well as double, absorber type. The thermal performance of these modified reverse flat-plate collectors is compared with that of a single absorber reverse flat-plate collector, as well as with the corresponding normal flat-plate collector. It is found that the new design having two absorbers gives the best thermal performance as compared with other configurations. The analytical models presented in this paper very well describe the experimental results.

Abstract The reverse flat-plate collector is a non-concentrating collector. It can collect solar heat at high temperatures which cannot be achieved by conventional non-concentrating collectors. In this paper, the authors have proposed a number of modified versions of the originally proposed reverse flat-plate collector. The new designs are of single, as well as double, absorber type. The thermal performance of these modified reverse flat-plate collectors is compared with that of a single absorber reverse flat-plate collector, as well as with the corresponding normal flat-plate collector. It is found that the new design having two absorbers gives the best thermal performance as compared with other configurations. The analytical models presented in this paper very well describe the experimental results.