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This work has been developed under the AGROinLOG Project, “Demonstration of innovative integrated biomass logistics centres for the Agro-industry sector in Europe”. An Integrated Biomass Logistics Center (IBLC), is based on the introduction of new production chains into existing agro-industries by using new biomass feedstock. The AGROinLOG Project has dedicated great attention to investigate the potential of cereal chaff as a valuable resource.Chaff is the fine fraction of the thrashing residues, not usually collected. Chaff is made up of glumes, seed husks, rachis and the tinner part of the cereal stems, whole and cracked kernels, as well as weed seeds.Currently there are several mechanical solutions available on the market for chaff recovery, and others are still at prototype stage, but theyare not so common and very often unknown to the farmers.So far, the literature reportsfew cases of chaff collection with the specific purpose of weed seeds removal, but it still lacks specificexperiments on these machinesintentionally used for biomass collection.For this reason, during the Project AGROinLOG a series of large field tests were performed using an independent scientific approach with different kind of chaff harvesting technologiesin France, Sweden and Italy from 2017 to 2019.The present study collects the results of these activities with the aim to fill that gap and provide deeper understanding in the possibility to enhance the current cereal harvesting method, in order to improve the quantity of biomass collected by including the chaff. Proceedings of the 29th European Biomass Conference and Exhibition, 26-29 April 2021, Online, pp. 62-68

Increasing agricultural productivity is one of the most important aims of modern biotechnology. One way to enhance the productivity of crop species is to enhance the efficiency of photosynthesis. In C3 plants the oxygenase activity of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) limits the photosynthetic efficiency. The green microalgae Chlamydomonas reinhardtii has evolved a CO2 concentration mechanism (CCM) by increasing the CO2 concentration in the chloroplast stroma where RuBisCO is located. Four independent transgenic tobacco genotypes (LA, LB, C1 and C3) were generated producing the low CO2-inducible protein A and B (LCIA and LCIB) or the carbonic anhydrases I and III (CAH1 and CAH3) from C. reinhardtii CCM in the envelope, stroma, intermembrane space or thylakoid lumen of tobacco chloroplasts, respectively. All four recombinant proteins were active in planta, which had a substantial impact on carbon and nitrogen metabolism. Increasing the CO2 concentration near RuBisCO resulted in an enhanced rate of photosynthesis (by up to 15%), efficiency of photosystem II (by up to 18%) and chlorophyll content (by up to 19%). Although to differing extents, all four transgenic genotypes grew faster than wild-type plants, produced more shoot biomass (up to 45% more fresh weight or 38% more dry weight in the LA lines) and accumulated more photosynthetic end products, reflecting the higher rate of photosynthetic CO2 fixation. The proteome analysis revealed that the proteins changed in the transgenic genotypes compared to the wild-type plants were primarily associated with the regulation of the Calvin cycle and the amino acid biosynthesis. Metabolic analysis of the transgenic LA, LB and C3 plants revealed an increase in the levels of carbohydrates and also of most amino acids. Furthermore, transgenic LA and LB plants could maintain the enhanced biomass under low nitrogen conditions, where similarly-treated wild-type plants grew more slowly. The data generated in the present study confirmed that even single Chlamydomonas CCM components can be integrated into C3 plants to increase biomass, suggesting that transgenic lines combining multiple components or even a complete CCM could further increase the productivity and yield of C3 crops. RWTH Aachen University, Diss., 2017; Aachen, 1 Online-Ressource (151 Seiten) : Illustrationen, Diagramme(2017). = RWTH Aachen University, Diss., 2017 Published by Aachen

Bio-based and biodegradable plastics (BBPs) are innovative materials, wholly or partially produced from biomass, with the potential to enhance the circulation of resources in the biological cycle of the Ellen MacArthur Foundation’s butterfly diagram. Although BBPs are generally considered more environmental-friendly than conventional plastics, robust scientific evidence is still missing. The lack of tools and metrics to assess the circularity and sustainability of the BBPs industry poses relevant challenges for its upscaling and contribution to climate neutrality goals in Europe. It calls for adopting system and life cycle thinking, guided by multi-level and multi-dimensional examinations, which led researchers to build a comprehensive picture of trends, gaps and future orientations that may boost a sustainable circular bioeconomy in the sector. The value- chain based and multi-faceted SWOT analysis that emerged from the intersection of system and corporate data reveals the need to establish a combined circular bioeconomy strategy where incentives to integrated local supply chain, dedicated EPR scheme, eco-design guidelines, revised EoL standards, new clear labelling schemes and harmonised sustainability criteria should be prioritized and conjointly pursued to accelerate the transition towards a sustainable circular bioeconomy of the BBPs value chain. European Journal of Social Impact and Circular Economy, V. 4 N. 2 (2023)

Ressourcen stellen die Basis für eine erfolgreiche industrielle und technologische Entwicklung dar und somit auch für den Wohlstand heutiger und zukünftiger Generationen. Mit steigender Ressourcennutzung nehmen auch die (physische und sozio-ökonomische) Verfügbarkeit abiotischer und biotischer Ressourcen, die Umweltverschmutzung und die sozialen Auswirkungen durch den Abbau und Nutzung der Ressourcen zu. Um den Erfolg implementierter Strategien (und deren Maßnahmen) hinsichtlich ihres Beitrags zu einem effizienten und nachhaltigen Umgang mit Ressourcen zu bewerten, bedarf es an entsprechenden Bewertungsmethoden. Diese Dissertation stellt vier Methoden bereit um die Bewertung abiotischer und biotischer Ressourcennutzung im Kontext der Nachhaltigkeit auf Produkt- und regionaler Ebene in konsistenter Weise zu bewerten. Die Methode zur Bewertung abiotischer Ressourcen auf Produktebene betrachtet insgesamt 21 relevante Aspekte und stellt Indikatoren zur Quantifizierung bereit. Für die Bewertung der sozio-ökonomischen Einschränkungen von Lieferketten ist eine neuer Ansatz entwickelt, der geopolitische, politische und regulative Aspekte berücksichtigt. Des Weiteren sind Screening-Indikatoren verfügbar, die die gesellschaftliche Akzeptanz der Ressourcennutzung adressieren. Um die Verfügbarkeit terrestrischer biotischer Ressourcen in Produktsystemen zu bewerten, wurde eine umfassende Methode mit 25 Indikatoren erstellt. Des Weiteren wird ein Ansatz vorgestellt, der es ermöglicht eine konsistente Zusammenführung und somit auch Bewertung verschiedener Ressourcentypen zu ermöglichen. Er findet bei der Zusammenführung der entwickelten Methoden zur Bewertung abiotischer und biotischer Ressourcen Anwendung. Da die Nutzung von Ressourcen auch auf Macro-Ebene betrachtet werden muss, wurde eine Methode zur Bewertung abiotischer Ressourcen auf regionaler Ebene entwickelt, die 25 Indikatoren für die Bewertung der Kritikalität (Verfügbarkeit von Ressourcen und Vulnerabilität der Region) und der gesellschaftlichen Akzeptanz zur Verfügung stellt. Verschiedene Fallstudien wurden durchgeführt um die Anwendbarkeit der entwickelten Methoden aufzuzeigen und zu verdeutlichen, warum eine umfassende Bewertung der Ressourcennutzung notwendig ist. Die Fallstudien umfassen u.a. die Bewertung eines Smartphones, Pkw-Herstellung und Biokraftstoffe. Die Anwendbarkeit der Methoden wird zudem erhöht, indem Indikatorwerte für 36 Metalle und 4 fossile Rohstoffe zur Verfügung gestellt werden. Die Bewertung der Nutzung abiotischer und biotischer Ressourcen auf Produkt- und regionaler Ebene wird mit dieser Dissertation signifikant verbessert, indem vier wissenschaftliche Methoden zur robusten und umfassenden Bewertung aller drei Nachhaltigkeitsdimensionen bereitgestellt werden. Resources are the basis for a thriving industrial and technological development and therefore for prosperity of present and future generations. With increasing resource use, challenges with regard to (physical and socio-economic) availability of abiotic and biotic resources and raw materials, pollution of the environment as well as social impacts associated with resource extraction and use arise. To evaluate the success of strategies managing resource use more efficiently and sustainably methodologies are required to comprehensively assess resource use and related impacts. This thesis provides four methodologies to improve the assessment of abiotic and biotic resource use in the context of sustainability on product and regional level. For the method to assess abiotic resources use on product level overall 21 aspects are considered as relevant and indicator for quantification are provided. In order to determine socio-economic supply chain restrictions a new approach is developed, considering geopolitical, political and regulatory aspects affecting resource extraction and use. Further, screening indicators are established to evaluate the societal acceptance of resources with regard to compliance with social and environmental standards. To assess the availability of terrestrial biotic resources in product systems a comprehensive methodology is established, which includes 25 indicators. Further, an approach is proposed to combine assessment methodologies in a consistent way. This approach is applied to the developed method of this thesis leading to a combined methodology. The use of resources also has to be considered on macro-economic. Thus, a methodology is developed providing 25 indicators for the two dimensions criticality, consisting of the sub-dimensions (physical and socio-economic) availability and vulnerability, as well as societal acceptance. Several case studies are carried out to demonstrate the applicability of the developed methods and to confirm the need for a comprehensive assess of resource use on micro and macro level, e.g. case studies for smart phones and cars, for biofuels produced from rapeseed and soybean. The applicability of the methodologies is further enhanced by providing indicator results for 36 metals and four fossil raw materials. The assessment of abiotic and biotic resource use on product and regional level is improved significantly by establishing four scientifically robust yet applicable methodologies, which consider multiple aspects of resource use in all three sustainability dimensions.

Die Metropolregion Santiago (MRS) verzeichnete in den letzten Jahren ein großes Bevölkerungswachstum und einen Anstieg des Lebensstandards. Als Folge davon hat sich das Aufkommen von Siedlungsabfällen fast innerhalb von 10 Jahren verdoppelt. Die Daten für den aktuellen Zustand des Abfallmanagements wurden durch Feldforschungen, Fragebögen, Feldbesuche und durch eine systematische Auswertung von bereits vorliegender Literatur erhoben. Das integrative Nachhaltigkeitskonzept der Helmholtz-Gemeinschaft diente als konzeptioneller Rahmen für die Studie. Zur Bewertung des aktuellen Zustands des Abfallmanagements wurden Nachhaltigkeitsindikatoren identifiziert, deren aktuelle Werte bestimmt und Zielwerte festgelegt. Die Nachhaltigkeitsanalyse zeigt, dass die größten Defizite darin liegen, dass nahezu die gesamte Abfallmenge ohne jegliche Vorbehandlung deponiert wird. Damit verbunden sind lang andauernde Emissionen von Treibhausgasen. Um herauszufinden, wie der informelle Sektor im Abfallmanagement zur Nachhaltigkeit beiträgt, wurden veröffentlichten Erfahrungen mit informellen Müllsammlern in Lateinamerika analysiert. Dabei wurden die entsprechenden Akteure sowie etablierte Allianzen zwischen diesen Akteuren identifiziert. Schlüsselfaktoren für ein nachhaltiges Abfallmanagement unter Einbeziehung des informellen Sektors sind die Legalisierung der Schattenwirtschaft und feste Verträge mit Partnerunternehmen. Relevante Akteure für die Gestaltung der Arbeitsbedingungen des informellen Sektors sind Vertreter privater und öffentlicher Unternehmen, einzelne gesellschaftliche Gruppen sowie Vertreter von Nichtregierungsorganisationen. Schließlich wurden drei explorative Szenarien für das Bezugsjahr 2030 entwickelt: Business as Usual (BAU), Collective Responsibility (CR) und Market Individualism (MI). Das BAU-Szenario enthält eine getrennte Sammlung von Bioabfall und von Wertstoffen; hervorgerufen durch einen verstärkten Organisationsgrad der informellen Müllsammler und den Ausbau von Bring-Systemen. Die Errichtung mechanischer Sortierungsanlagen trägt zur Verwertung der Materialien und zum Recycling bei. Das entstehende Deponiegas und Biogas wird als erneuerbare Energiequelle genutzt. Das CR-Szenario enthält eine getrennte Sammlung von Bioabfall und Wertstoffen. Dies wird erreicht durch eine verstärkte Zusammenarbeit mit den jetzt organisierten Müllsammlern und durch den Ausbau von Bring-Systemen. Mechanische Sortierungsanlagen tragen zur Verwertung von Materialien und zum Recycling bei. Durch Abtrennung einer heizwertreichen Fraktion in mechanisch biologischen Anlagen werden Sekundärbrennstoffe produziert. Darüber hinaus werden, das entstehende Deponiegas sowie das in Vergärungsanlagen erzeugte Biogas energetisch genutzt. Im MI-Szenario sind Wiederverwertungsstrategien von untergeordneter Bedeutung. Es gibt kein Interesse an einer Zusammenarbeit mit den informellen Müllsammlern und keine Anreize für einen verstärkten Organisationsgrad in diesem Bereich. Deshalb bleibt die Branche weitgehend informell. Technologische Entwicklungen in diesem Szenario enthalten die mechanische Sortierung von gemischtem Abfall und die energetische Verwertung von Deponiegas. Die Ergebnisse zeigten, dass das Pro-Kopf-Aufkommen an Siedlungsabfällen im Jahr 2030 in allen Szenarien deutlich höher als im Jahr 2007 ist und der festgelegte Zielwert von 1,6 kg/Kopf/Tag nicht erreicht wurde. Den höchsten Wert (2,0 kg/Kopf/Tag) weist das MI-Szenario auf, der niedrigste Wert (1,8 kg/Kopf/Tag) wurde im CR-Szenario gefunden. Eine Vorbehandlung der gesammelten gemischten Siedlungsabfälle findet nur im CR-Szenario statt, der entsprechende Wert beträgt 18 %, der Zielwert wird damit nicht erreicht. Die höchsten Treibhausgasemissionen treten im MI-Szenario (295 kg CO2-eq/Kopf/Jahr) auf, den niedrigsten Wert (155 kg CO2-eq/Kopf/Jahr) findet man im CR-Szenario. All diese Werte sind, verglichen mit dem festgelegten Zielwert von 71 kg/Kopf/Jahr, deutlich zu hoch. Der Zielwert für die Wiederverwertungsquote wurde im CR-Szenario erreicht (43 %), den niedrigsten Wert zeigt das MI-Szenario (20 %). Die Zielwerte für das Einkommen der Müllsammler wurden im CR-Szenario erreicht (128 %). Im MI-Szenario beträgt dieser Wert lediglich 51 %. Die Kosten für das Abfallmanagement im Verhältnis zum Bruttoinlandsprodukt sinken in den drei Szenarien. Aus den Ergebnissen lässt sich ableiten, dass ein Einbeziehen von mehreren Faktoren erforderlich ist, um die Nachhaltigkeit des Abfallmanagementsystems in den drei Szenarien zu steigern und dass es von wesentlicher Bedeutung ist, schon vorhandene und gut funktionierende Subsysteme, wie das der informellen Müllsammler, zu nutzen und zu stärken. Ebenso ist die Umsetzung robuster Behandlungstechnologien, die einen Beitrag zur Reduktion negativer Umweltauswirkungen leisten, zu forcieren. Diese Technologien sollten preiswert sein, um ihren Einsatz auch unter wirtschaftlich vertretbaren Gesichtspunkten zu ermöglichen. The Metropolitan Region of Santiago (MRS) has experienced a large growth in population in recent years and a rise in the standard of living. Therefore, its municipal solid waste (MSW) has almost doubled in 10 years. Data about the current situation of MSW management in MRS were collected during field research, interviews, field visits and by a systematic evaluation of existing documentary literature. The Integrative Sustainability Concept of the Helmholtz Association provided a conceptual framework for the study. The sustainability analysis showed that the largest deficits are in the current amount of MSW deposited at sanitary landfills without any pre-treatment, and the emission values of greenhouse gases associated with waste treatment and final disposal. To find out if and how the informal waste sector contributes to sustainability, experiences of organization of informal primary collectors in Latin America were analyzed. The key factors which have an influence on their working conditions were identified. These factors include the existence of a legal framework for the informal waste sector; the existence of alliances with production companies guaranteeing a reliable industrial market for secondary raw materials and expansion of activities beyond collection of recyclables. Key stakeholders included people from the public and the private sector, from the civil society and from NGOs. Three explorative scenarios were developed for the year 2030: Business as Usual (BAU), Collective Responsibility (CR), and Market Individualism (MI). Waste generation, waste composition and different practices of waste collection, recovery and treatment were taken into account for the scenarios formulation. The BAU scenario incorporated separate collection of biowaste, recyclable materials with some participation of organized primary collectors and an expansion of drop-off systems. The mechanical sorting of mixed waste was introduced. The utilization of landfill gas as an energy source was promoted and the production of biogas in anaerobic digestion plants was implemented. The CR scenario incorporated separate collection of biowaste, commitment to work together with the primary waste collectors and an expansion of drop-off systems. The mechanical and mechanical biological treatment of mixed waste was introduced. The utilization of landfill gas as an energy source is promoted and the production of biogas in anaerobic digestion plants was implemented. In the MI separate collection of biowaste and recyclable materials was irrelevant. An organization of the informal primary collectors did not take place. Mechanical sorting of mixed waste was introduced. Utilization of landfill gas as an energy source was promoted. The results showed that the generation flux of MSW is at least 50% larger in all scenarios in 2030 compared to the year 2007, exceeding the limit value proposed. The highest value (2.0 kg/(person•day) is obtained in the MI scenario, and the lowest (1.8 kg/(person•day)) in the CR scenario. Pre-treatment of mixed MSW collected is only achieved in the CR scenario with a value of 18%, however, the target value is not achieved. The highest greenhouse gas emission value is obtained in the MI scenario with 295 kg CO2eq/(person•year), the lowest value of 155 kg CO2-eq/(person•year) is obtained in the CR scenario; a value that is still very high in comparison with the suggested target. The largest recycling rate is obtained in the CR scenario (43%), which is better than the target value proposed, the lowest recycling rate is obtained in the MI scenario (20%). The income of primary collectors in comparison with the income of one individual household is improved significantly in the CR scenario (128%), in the MI scenario, earnings of primary collectors decreased to 51%. The share of GDP spent on MSW management is lower in 2030, compared to the year 2007, in all scenarios the largest value of 0.17% is obtained in the CR scenario, and the lowest value of 0.14% is obtained in the MI scenario. The results of the evaluation of the scenarios showed that the largest sustainability deficits are the amount of mixed MSW which undergoes pre-treatment, the greenhouse gas emissions associated to MSW treatment and disposal, as well as the share of GDP spent on MSW management. The results obtained suggested that an integration of several factors is required to increase sustainability. It is essential to strengthen and take advantage of the subsystems which are working within the waste management system, as in the case of the informal sector. In addition to the implementation of flexible treatment technologies which help to decrease negative environmental impacts. Moreover, the costs of these technologies should be affordable, allowing a better financial management.

A reduction in the availability of phosphate rock resources for fertiliser production coincides with an increase in phosphorus-rich dairy wastewater in Europe. This confluence of events has led to the development of technologies for phosphorus recovery from dairy wastewater and the use of the products as fertilisers in agriculture. This thesis aims to contribute both to the technical development of this emerging technical system and methodological development of assessing the sustainability of it with regard to (1) the identification and selection of sustainability indicators, and (2) the assessment of life cycle environmental impacts. The thesis describes an approach developed for identifying and selecting sustainability indicators by reviewing scientific documents and interviews as well as an approach employed for performing a meta-analysis of previously published life cycle assessment results to cope with lack of inventory data. The employed method for indicator selection narrows down an initial set of 382 sustainability indicators identified in the literature to 26, which were considered representative and useful for the assessment of the considered innovative conceptual system. The meta-analysis results suggested that the examined phosphorus recovery technologies exhibited a lower global warming potential and cumulative energy demand than those of dairy wastewater treatment processes and that those technologies recovering phosphorus from the liquid phase had lower impacts than those recovering phosphorus from sludge or ash.

The core aim of this thesis was to quantify the effects of co-digesting forage silages with animal slurries on methane yields and to investigate if antagonistic or synergistic outcomes occur. In order to complete this assessment, the economic impacts of changing forage silage characteristics, of changing the mixing ratios of forage silage and cattle slurry in binary mixtures (and the presence of synergy or antagonism) and of changing the costs of providing these feedstocks for anaerobic digestion (AD) on the cost of methane production in an on-farm AD facility were accessed. An initial objective, however, was to define an optimal methodology for laboratory-scale anaerobic digestion, specifically to determine the impact of altering the headspace volume within incubation bottles and the overhead pressure measurement and release (OHPMR) frequency on methane yield using a manual manometric biochemical methane potential (mBMP) batch digestion method. Two anaerobic batch co-digestion experiments were conducted with forage silages and animal slurries. In the first experiment, oven-dried perennial ryegrass (harvested at two growth stages) or red clover (harvested at two growth stages) silages and cattle slurry were co-digested. Each binary mixture had synergistic effects which resulted in 2.8-7.5% higher methane yields than predicted from mono-digestion of individual substrates. In the second experiment, cattle slurry (two types) or pig slurry was co-digested with undried perennial ryegrass silages (harvested at two growth stages). Each silage and slurry mixture had antagonistic effects which resulted in methane yields 5.7-7.6% below those predicted from mono-digestion of individual substrates. In the initial experiment and in order to broaden the conditions under which the assessment was made, the biogas and methane yields of cellulose, barley grain, grass silage and cattle slurry were determined in response to three incubation bottle headspace volumes and four OHPMR frequencies. The methane yields of barley, silage and slurry were also compared with those from an automated volumetric method (i.e. AMPTS). Headspace volume and OHPMR frequency effects on biogas yield were mediated mainly through headspace pressure, with the latter having a negative effect on the biogas yield measured but relatively little effect on methane yield. Two mBMP treatments that produced methane yields equivalent to AMPTS were identified. Economic modelling results showed significant impacts of AD feedstock characteristics and their provision cost on the cost of methane production in an AD facility. The feedstock provision cost contributed about half of the total cost of methane production when the AD facility solely operated on grass silage. The total cost of methane produced from mono-digestion of cattle slurry that was supplied free of charge was more than double the cost of methane produced from grass silage. For co-digestion of grass silage and cattle slurry, the total cost of methane production progressively increased as the proportion of slurry in the co-digested mixture increased. Antagonistic and synergistic methanogenesis resulted in a corresponding 6% higher and 5% lower total cost of methane production during co-digestion of grass silage and cattle slurry (at a silage:slurry volatile solids ratio of 0.8:0.2) compared to the binary mixture without these effects.

We analyse ex-ante the effects of diet recommendations in France, Finland and Denmark. The simulation approach combines a behavioural model of adjustment to dietary constraints, an epidemiological model and an LCA model. We conclude that for the three countries: 1- The promotion of several diet recommendations would improve social welfare; 2- Healthy-eating recommendations targeting consumption of saturated fat, fruits/vegetables and salt should be prioritized for promotion; 3- Although synergies dominate, trade-offs between environmental and health objectives may occur in Finland and Denmark; and 4- The taste/utility cost of dietary change imposed on consumers should be included in the welfare analysis of diet recommendations. none