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Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the present work, it was found that the presence of calcium promotes selective growth and enrichment of the PHA-storing biomass fraction and significantly improved both PHA contents and yields. Calcium addition resulted in PHA contents of 0.60 ± 0.03 gPHA/gVSS and average PHA yields on substrate of 0.49 ± 0.03 gCODPHA/gCODHAc compared to 0.35 ± 0.01 gPHA/gVSS and 0.19 ± 0.01 gCODPHA/gCODHAc without calcium addition. After 48 h, three times more PHA was produced compared to control experiments without calcium addition. Higher PHA content and selective biomass production is proposed to be a consequence of calcium dependent increased levels of passive acetate uptake. Such more efficient substrate uptake could be related to a formation of calcium acetate complexes. Findings lead to bioprocess methods to stimulate a short-term selective growth of PHA-storing microorganisms and this enables improvements to the techno-economic feasibility for municipal waste activated sludge to become a generic resource for industrial scale PHA production.

This Article examines the genesis and context of SE4All, placing the effort within both itshistorical and international policy contexts. It highlights the voluntary nature of the initiative andargues that its effective implementation and the achievement of its goals require the articulation ofan applicable international legal framework that aids the transformation of SE4All’s policy actionsinto binding international legal commitments. The article contends that such a transformation doesnot depend on the creation of entirely new legal rules or institutions. Instead, an effective frame-work for successful implementation of SE4All can be derived from existing rules of internationalhuman rights law and sustainable development law. Reliance on these twin bodies of interna-tional law will increase the prospects for SE4All to achieve energy access and related goals thatits predecessor initiatives have failed to accomplish

The design and assessment of net-zero buildings commonly focus exclusively on the operational phase, ignoring the embodied environmental impacts over the building life cycle. An analysis is presented on the consequences of integrating embodied impacts into the assessment of the environmental advantageousness of net-zero concepts. Fundamental issues needing consideration in the design process - based on the evaluation of primary energy use and related greenhouse gas emissions - are examined by comparing three net-zero building design and assessment cases: (1) no embodied impacts included, net balance limited to the operation stage only; (2) embodied impacts included but evaluated separately from the operation stage; and (3) embodied impacts included with the operation stage in a life cycle approach. A review of recent developments in research, standardization activities and design practice and the presentation of a case study of a residential building in Norway highlight the critical importance of performance indicator definitions and system boundaries. A practical checklist is presented to guide the process of incorporating embodied impacts across the building life cycle phases in net-zero design. Its implications are considered on overall environmental impact assessment of buildings. Research and development challenges, as well as recommendations for designers and other stakeholders, are identified.

Common ragweed (Ambrosia artemisiifolia L.) is a highly allergenic annual ruderal plant and native to Northern America, but now also spreading across Europe. Air pollution and climate change will not only affect plant growth, pollen production and duration of the whole pollen season, but also the amount of allergenic encoding transcripts and proteins of the pollen. The objective of this study was to get a better understanding of transcriptional changes in ragweed pollen upon NO2 and O3 fumigation. This will also contribute to a systems biology approach to understand the reaction of the allergenic pollen to air pollution and climate change. Ragweed plants were grown in climate chambers under controlled conditions and fumigated with enhanced levels of NO2 and O3. Illumina sequencing and de novo assembly revealed significant differentially expressed transcripts, belonging to different gene ontology (GO) terms that were grouped into biological process and molecular function. Transcript levels of the known Amb a ragweed encoding allergens were clearly up-regulated under elevated NO2, whereas the amount of allergen encoding transcripts was more variable under elevated O3 conditions. Moreover transcripts encoding allergen known from other plants could be identified. The transcriptional changes in ragweed pollen upon elevated NO2 fumigation indicates that air pollution will alter the transcriptome of the pollen. The changed levels of allergenic encoding transcripts may have an influence on the total allergenic potential of ragweed pollen.

Higher Education Institutions are facing a challenging situation: how to introduce concepts such as Sustainability or the Circular Economy into their curricula. This study presents how to organize a Computer Reuse Program, an educational proposal for the Reuse, Repair, Refurbishment, Remanufacture and Recycling of computers into a Curriculum and a case study. The proposal is based in the Service-Learning methodology, by which students develop technical and professional skills while undertaking a project that has a direct and real impact on society. Students work on old or broken computers provided by donors, thereby acquiring technical skills. These now flawlessly functioning computers are donated to NGOs and other non-profit organizations, thus endowing the equipment with a much longer life as well as reducing e-waste, one of the fastest-growing waste streams in the world. As a case study, this paper presents the UPC Computer Reuse Program, carried out at Universitat Politècnica de Catalunya UPC-BarcelonaTech. Since the program started in 2004, some 2500 computers have been donated to 359 different organizations in 29 countries, and more than 5200 students have participated. The paper analyzes the impact of the program on society, on the reduction of e-waste, on the environment and on student awareness regarding social justice and sustainability.

Abstract Biofuels are considered as an alternative to partially replace fossil fuels and mitigate climate change effects. A life cycle assessment of second generation ethanol, derived from banana agricultural wastes, was developed to assess its environmental sustainability and demonstrate its capacity of reducing greenhouse gas emissions. The methodological approach was conducted in a Well-to-Wheel perspective, using as functional unit 1 MJ of energy released in the combustion of bioethanol in a passenger car from different bioethanol blends. Primary and secondary information sources were used for the assessment; mass balance and ethanol yield data came from laboratory experimentation. The environmental assessment was carried out using SimaPro 8.0.4.30 with the ReCiPe midpoint (H) impact assessment methodology. The quantified impact categories were climate change (CC), terrestrial acidification (TA), freshwater eutrophication (FE), photochemical oxidant formation (PO), particulate matter formation (PM), and fossil depletion (FD). In addition, net energy value and energy ratio (ER) were analyzed to ensure a positive energy balance. Compared to using pure gasoline, blended gasoline reduced CC, PO, PM, and FD impacts, but increased FE and TA impacts. The obtained energy balance was positive, with an ER of 2.68 MJ/MJ. Wastewater treatment is the process that presented the greatest energy consumption. Since Ecuador is the world's largest exporter of bananas, and a great amount of agricultural waste is available, a case study in this country was analyzed. This case study indicated that Ecuador could use banana residue for ethanol production, considering its positive and negative impacts. In conclusion, second generation ethanol derived from banana agricultural waste has potential to reduce greenhouse gas emissions and fossil depletion and has a positive energy balance.

(1) The amount of plastic discharges in the environment has drastically increased in the last decades negatively affecting aquatic ecosystems, societies, and the world economy. The policies initiated to deal with this problem are insufficient and there is an urgency to initiate local actions based on a deep understanding of the factors involved. (2) This paper investigates the potential of massive open online courses (MOOCs) to spread environmental education. Therefore, the conclusions drawn from the implementation of a MOOC to combat the problem of marine litter in the world are presented. (3) This work describes the activity of 3632 participants from 64 countries taking an active role presenting useful tools, connecting them with the main world associations, and defining applied action plans in their local area. Pre- and post-questionnaires explore behavioral changes regarding the actions of participants to combat marine litter. The role of MOOCs is contrasted with social media, formal education, and informal education. (4) Findings suggest that MOOCs are useful instruments to promote environmental activism, and to develop local solutions to global problems, for example, clean beaches, supplanting plastic bottles, educational initiatives, and prohibition of single-use plastic.