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This dataset contains measures of fitness traits from Eschscholzia californica progeny which were experimentally supplemented with selfed or outcrossed pollen to determine the effects of self-fertilisation on a plant which has a low propensity to self. A glasshouse experiment was conducted using 40 plants. On each plant two flowers were emasculated and the first supplemented with outcrossed pollen and the second with self-pollen. From each supplemented plant, a seed was sowed from the outcrossed fruit and from the selfed fruit. The following fitness traits were recorded; the germination rate, the duration from germination to reproductive maturity (time of first flower), together with the height (cm) and biomass (number of flowers and buds) at reproductive maturity. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants. We performed a glasshouse experiment using 40 artificially crossed plants. On each plant, we emasculated two flowers and supplemented the first with outcrossed pollen and the second with self-pollen. This involved methodically wiping two dehiscing anthers from a donor plant or the focal plant onto the receptive stigma with dissecting tweezers, before covering it in fine muslin. From each supplemented plant, we sowed a seed from the outcrossed fruit and from the selfed fruit (given that selfed fruits predominantly only produced one seed) into 1L pots. These were then stored under glasshouse conditions before the fitness traits were measured.

Global change drivers that modify the quality and quantity of litter inputs to soil affect greenhouse gas fluxes, and thereby constitute a feedback to climate change. Carbon cycling in the Yukon-Kuskokwim (Y-K) River Delta, a subarctic wetland system, is influenced by landscape variations in litter quality and quantity generated by herbivores (migratory birds) that create ‘grazing lawns’ of short stature, nitrogen-rich vegetation. To identify the mechanisms by which these changes in litter inputs affect soil carbon balance, we independently manipulated qualities and quantities of litter representative of levels found in the Y-K Delta in a fully factorial microcosm experiment. We measured carbon dioxide (CO2) fluxes from these microcosms weekly. To help us identify how litter inputs influenced greenhouse gas fluxes, we sequenced soil fungal and bacterial communities, and measured soil microbial biomass carbon, dissolved carbon, inorganic nitrogen, and enzyme activity. We found that positive correlations between litter input quantity and CO2 flux were dependent upon litter type, due to differences in litter stoichiometry and changes to the structure of decomposer communities, especially the soil fungi. These community shifts were particularly pronounced when litter was added in the form of herbivore feces, and in litter input treatments that induced nitrogen limitation (i.e., senesced litter). The sensitivity of carbon cycling to litter quality and quantity in this system demonstrates that herbivores can strongly impact greenhouse gas fluxes through their influence on plant growth and tissue chemistry.

This dataset includes the results summary from a lab-scale bioreactor experiment as discussed in the research paper with the same name, published at Processes MDPI (De Groof, V.; Coma, M.; Arnot, T.C.; Leak, D.J.; Lanham, A.B. Adjusting Organic Load as a Strategy to Direct Single-Stage Food Waste Fermentation from Anaerobic Digestion to Chain Elongation. Processes 2020, 8, 1487.). The study comprised two operational phases of duplicate reactors fed with food waste, each set to target a different product. The data comprises a summary on feedstock composition, microbial community analysis and operational conditions and product outcome per operational phase. The archaeal and bacterial community data includes the final sequences of the operational taxonomic units found and their relative abundance in each sample as determined by 16s rRNA amplicon sequencing. The raw data files have been submitted in the specialized EMBL-EBI database and are available under the accession number PRJEB39281. This dataset was prepared and processed in Microsoft Excel from raw analytical data. The bioinformatic processing prior to the microbial community summary in the spreadsheet was done as outlined in the publication, and results were processed via the DNASense data analysis app (applies Rstudio IDE v.3.5.1 with the ampvis v.2.5.8. package). This version includes rarefaction curves and values of alpha-diversity, richness and evenness per sample in the OTU_table tab. Analytical

Datasets for the paper Richard, B. et al. The climatic debt is growing in the understory of temperate forests: stand characteristics matter. Global Ecology and Biogeography. The files provided here contain the data for CTI and contributing factors in lags used in analyses.

Project: Coupled Model Intercomparison Project Phase 6 (CMIP6) datasets - These data have been generated as part of the internationally-coordinated Coupled Model Intercomparison Project Phase 6 (CMIP6; see also GMD Special Issue: http://www.geosci-model-dev.net/special_issue590.html). The simulation data provides a basis for climate research designed to answer fundamental science questions and serves as resource for authors of the Sixth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC-AR6). CMIP6 is a project coordinated by the Working Group on Coupled Modelling (WGCM) as part of the World Climate Research Programme (WCRP). Phase 6 builds on previous phases executed under the leadership of the Program for Climate Model Diagnosis and Intercomparison (PCMDI) and relies on the Earth System Grid Federation (ESGF) and the Centre for Environmental Data Analysis (CEDA) along with numerous related activities for implementation. The original data is hosted and partially replicated on a federated collection of data nodes, and most of the data relied on by the IPCC is being archived for long-term preservation at the IPCC Data Distribution Centre (IPCC DDC) hosted by the German Climate Computing Center (DKRZ). The project includes simulations from about 120 global climate models and around 45 institutions and organizations worldwide. Summary: These data include the subset used by IPCC AR6 WGI authors of the datasets originally published in ESGF for 'CMIP6.C4MIP.MOHC.UKESM1-0-LL' with the full Data Reference Syntax following the template 'mip_era.activity_id.institution_id.source_id.experiment_id.member_id.table_id.variable_id.grid_label.version'. The UKESM1.0-N96ORCA1 climate model, released in 2018, includes the following components: aerosol: UKCA-GLOMAP-mode, atmos: MetUM-HadGEM3-GA7.1 (N96; 192 x 144 longitude/latitude; 85 levels; top level 85 km), atmosChem: UKCA-StratTrop, land: JULES-ES-1.0, ocean: NEMO-HadGEM3-GO6.0 (eORCA1 tripolar primarily 1 deg with meridional refinement down to 1/3 degree in the tropics; 360 x 330 longitude/latitude; 75 levels; top grid cell 0-1 m), ocnBgchem: MEDUSA2, seaIce: CICE-HadGEM3-GSI8 (eORCA1 tripolar primarily 1 deg; 360 x 330 longitude/latitude). The model was run by the Met Office Hadley Centre, Fitzroy Road, Exeter, Devon, EX1 3PB, UK (MOHC) in native nominal resolutions: aerosol: 250 km, atmos: 250 km, atmosChem: 250 km, land: 250 km, ocean: 100 km, ocnBgchem: 100 km, seaIce: 100 km.

Project: Coupled Model Intercomparison Project Phase 6 (CMIP6) datasets - These data have been generated as part of the internationally-coordinated Coupled Model Intercomparison Project Phase 6 (CMIP6; see also GMD Special Issue: http://www.geosci-model-dev.net/special_issue590.html). The simulation data provides a basis for climate research designed to answer fundamental science questions and serves as resource for authors of the Sixth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC-AR6). CMIP6 is a project coordinated by the Working Group on Coupled Modelling (WGCM) as part of the World Climate Research Programme (WCRP). Phase 6 builds on previous phases executed under the leadership of the Program for Climate Model Diagnosis and Intercomparison (PCMDI) and relies on the Earth System Grid Federation (ESGF) and the Centre for Environmental Data Analysis (CEDA) along with numerous related activities for implementation. The original data is hosted and partially replicated on a federated collection of data nodes, and most of the data relied on by the IPCC is being archived for long-term preservation at the IPCC Data Distribution Centre (IPCC DDC) hosted by the German Climate Computing Center (DKRZ). The project includes simulations from about 120 global climate models and around 45 institutions and organizations worldwide. Summary: These data include the subset used by IPCC AR6 WGI authors of the datasets originally published in ESGF for 'CMIP6.DAMIP.CSIRO.ACCESS-ESM1-5.hist-nat' with the full Data Reference Syntax following the template 'mip_era.activity_id.institution_id.source_id.experiment_id.member_id.table_id.variable_id.grid_label.version'. The Australian Community Climate and Earth System Simulator Earth System Model Version 1.5 climate model, released in 2019, includes the following components: aerosol: CLASSIC (v1.0), atmos: HadGAM2 (r1.1, N96; 192 x 145 longitude/latitude; 38 levels; top level 39255 m), land: CABLE2.4, ocean: ACCESS-OM2 (MOM5, tripolar primarily 1deg; 360 x 300 longitude/latitude; 50 levels; top grid cell 0-10 m), ocnBgchem: WOMBAT (same grid as ocean), seaIce: CICE4.1 (same grid as ocean). The model was run by the Commonwealth Scientific and Industrial Research Organisation, Aspendale, Victoria 3195, Australia (CSIRO) in native nominal resolutions: aerosol: 250 km, atmos: 250 km, land: 250 km, ocean: 100 km, ocnBgchem: 100 km, seaIce: 100 km.

This spreadsheet contains nine tabs to present the data used in the article 'Microclimate-driven trends in spring-emergence phenology in a temperate reptile (Vipera berus): Evidence for a potential ‘climate trap’?' (Turner & Maclean, 2022; Ecology and Evolution). The first tab, labelled 'Metadata_README', contains metadata for the dataset, including identification and affliliations of the authors, a description of the tabs in the spreadsheet, and descriptions of data labels used in the spreadsheet tabs. The second tab, labelled 'adder_sightings', comprises of records of Vipera berus (adder) sightings in Cornwall, United Kingdom, sourced from the Environmental Records Centre for Cornwall and the Isles of Scilly (www.erccis.org.uk), the Record Pool (www.recordpool.org.uk) and the Cornish Biodiversity Network (www.cornishbiodiversitynetwork.org). Due to data sensitivities and issues associated with the General Data Protection Regulation, information pertaining to the locations and dates of adder sightings in some instances in the dataset have only be provided at reduced spatial and temporal resolutions. A unique identification code for locations has been attributed to records. For full-resolution access, contact the data custodian and corresponding author. The raw datasets of adder sightings were filtered prior to inclusion in the analysis in Turner and Maclean. See the main text for all filtering procedures and microclimate modelling. The remaining tabs contain data relating to each adder sighting location in 'adder_sightings' for each year 1983 - 2017 computed from microclimate models using the microclima R package (Maclean et al., 2019). The third tab, labelled 'total_spring_frost', contains annual rates of spring ground frost. The fourth, fifth and sixt tabs, labelled 'Cue1(i)', "Cue1(ii)', and 'Cue1(iii)', each contain predicted annual adder emergence timing and computed rates of post-emergence spring ground frost using the 5th, 2.5th and 10th percentile thresholds, respectively, of an accumulated (degree-hours) temperature cue for adder emergence. The seventh tab, labelled 'Cue2', contains predicted annual adder emergence timing and computed rates of post-emergence spring ground frost using a sharp rise in accumulated (degree-hours) temperature cue for adder emergence. The eighth tab, labelled 'Cue3', contains predicted annual adder emergence timing and computed rates of post-emergence spring ground frost using a below-ground temperature gradient collapse cue for adder emergence. Lastly, the ninth tab, labelled 'Cue4', contains predicted annual adder emergence timing and computed rates of post-emergence spring ground frost using a critical air temperature (10°C) cue for adder emergence. The main text presents the analysis of adder emergence and spring ground frost data from the 'Cue1(i)'. Analysis of data from 'Cue1(ii)', 'Cue1(iii)', 'Cue2', 'Cue3', and 'Cue4' are presented in the Supplementary Information for Turner and Maclean. Climate change will increase the exposure of organisms to higher temperatures, but can also drive phenological shifts that alter their susceptibility to conditions at the onset of breeding cycles. Organisms rely on climatic cues to time annual life-cycle events, but the extent to which climate change has altered cue reliability remains unclear. Here, we examine the risk of a ‘climate trap’ – a climatically-driven desynchronisation of the cues that determine life-cycle events and fitness later in the season in a temperate reptile, the European adder (Vipera berus). During the winter, adders hibernate underground, buffered against sub-zero temperatures, and re-emerge in the spring to reproduce. We derived annual spring-emergence trends between 1983 and 2017 from historical observations in Cornwall, United Kingdom, and related these trends to the microclimatic conditions that adders experienced. Using a mechanistic microclimate model, estimates of below- and near-ground temperatures were used to derive accumulated degree-hour and absolute temperature thresholds that predicted annual spring-emergence timing. Trends in annual emergence timing and subsequent exposure to ground frost were then quantified. We found that adders have advanced their phenology towards earlier emergence. Earlier emergence was associated with increased exposure to ground frost and, contradicting the expected effects of macroclimate warming, increased post-emergence exposure to ground frost at some locations. The susceptibility of adders to this ‘climate trap’ was related to the rate at which frost risk diminishes relative to advancement in phenology, which depends on the seasonality of climate. We emphasise the need to consider exposure to changing microclimatic conditions when forecasting biological impacts of climate change.

Output data prepared for analysis of 25 diverse global cities by the Global Healthy and Sustainable City-Indicator Collaboration study, published in The Lancet Global Health Series on urban design, transport, and health. 2022. https://www.thelancet.com/series/urban-design-2022 Boeing, G. et al. (2022) ‘Using open data and open-source software to develop spatial indicators of urban design and transport features for achieving healthy and sustainable cities’, The Lancet Global Health, 10(6), pp. e907–e918. Available at: https://doi.org/10.1016/S2214-109X(22)00072-9. Data outputs were generated through use of the global-indicators software tool, designed for this study and available from: https://github.com/global-healthy-liveable-cities/global-indicators Further detail on the methods used is provided in the following publication: Liu, S., Higgs, C., Arundel, J., Boeing, G., Cerdera, N., Moctezuma, D., Cerin, E., Adlakha, D., Lowe, M. and Giles-Corti, B. (2021), A Generalized Framework for Measuring Pedestrian Accessibility around the World Using Open Data. Geogr Anal. https://doi.org/10.1111/gean.12290 The study made use of OpenStreetMap, Global Human Settlements and custom data, and is made available under the Open Database License: http://opendatacommons.org/licenses/odbl/1.0/. Any rights in individual contents of the database are licensed under the Database Contents License: http://opendatacommons.org/licenses/dbcl/1.0/