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Hydrochar, carbon-rich material produced during the thermochemical processing of biomass, is receiving increased attention due to its potential value as soil amendment. It can increase agroforestry systems’ productivity through direct and indirect effects on growth and soil quality. Hydrochar may also directly help mitigate climate change by sequestering stable carbon compounds in the soil and perhaps indirectly through increased C uptake by trees. In this research, we aim to evaluate how the application of hydrochar produced by two feedstock types, Cynara cardunculus L. (Hc) residuals and sewage sludge (Hs), and in two different doses (3 and 6 kg m−2) could improve the growth and water use efficiency of Populus alba L., a fast-growing tree species largely used in agroforestry as bioenergy crops and in C sequestration. We considered five plants per treatment, and we measured apical growth, secondary growth, leaf area and intrinsic water use efficiency in each plant for the whole growing season from February to October 2022. Our results highlighted that hydrochar applications stimulate the growth and water use efficiency of plants and that the double dose (6 kg m−2) of both hydrochars, and particularly Hc, had positive effects on plant performance, especially during extremely hot periods. Indeed, the year 2022 was characterized by a heat wave during the summer period, and this condition allowed us to evaluate how plants, growing in soils amended with hydrochar, could perform under climate extremes. Our findings showed that the control plants experienced severe damage in terms of dried stems and dried leaves during summer 2022, while hydrochar applications reduced these effects.

In the transition from linear production systems, unsustainable from the point of view of resources, to a model that finds strength in environmental, social and economic sustainability, the circular economy paradigm is the foundation that facilitates the planetary agro-ecological transition. The European Union has taken a number of steps (including the Circular Economy Package of Directives) shaping circularity as a wide-ranging driver measure involving many sectors. The paper intends to provide a regulatory framework on the current general situation regarding circularity in European Union, in order to extrapolate and give evidence to the aspects that intersect the agri-food sector. This is not only because they are poorly addressed in the literature, but also because there is a lack of regulatory instruments on the circular economy specifically addressing this area of interest. For this purpose, the analysis focuses on waste and residue/scrap management issues, recognized by law as by-products and end-of-waste status, as they are covered by circular economy legislation and as they can be applied to the agri-food sector. The latter allow the implementation of circularity strategies in the agri-food sector and, given the numerousness of production chains and the peculiarities of each of them, various regeneration and/or reuse processes of specific resources may be depicted. The intent is to provide useful knowledge on how to implement sustainable waste management, also proposing a concrete case on a by-product of olive oil processing, through which it is possible to highlight how the correct application of regulations favors the adoption of circular economic and management models in the firms involved, as well as informing the relevant economic operators on the possible profiles of legal liability that may arise from insufficient knowledge. Furthermore, this paper delves into the European Green Deal’s Strategy as it enriches the circular economy paradigm with new facets. NextGenerationEU and the National Recovery and Resilience Plan financially support this strategy in the aftermath of the socioeconomic crisis from COVID-19 in the EU Member States. This is in order to achieve the objective of achieving the agro-ecological transition.

In the agricultural sector, climate change (CC) affects multiple weather variables at different stages of crop cycles. CC may influence the mean level or affect the distribution of events (e.g., rainfall, temperature). This work evaluates the economic impact of CC-related changes in multiple climatic components, and the resulting uncertainty. For this purpose, a three-stage discrete stochastic programming model is used to represents farm sector of an irrigated area of Italy and to examine the influence of CC on rainfall and on maximum temperature. These variables affect the availability of water for agriculture and the water requirements of irrigated crops. The states of nature, and their change, are defined more broadly than in previous analyses; this allows examining the changes of more climatic variables and crops cultivation. The effect of CC is obtained by comparing the results of scenarios that represent the climatic conditions in the current situation and in the future. The results show that the agricultural sector would seek to lower costs by modifying patterns of land use, farming practices and increasing the use groundwater. The overall economic impact of these changes is small and due primarily to the reduced availability of water in the future. The temperature increase is, in fact, largely offset by the effects of the increase in CO2 levels, which boosts the yield of main crops of the irrigated zone. Therefore, availability and water management becomes a crucial factor to offset the increase of evapotranspiration and of water stress resulting from the increase of temperature. However, the costs of CC are very high for some types of farming, which suffer a large reduction in income.

With an approach combining crop modelling and biotechnology to assess the performance of three durum wheat cultivars (Creso, Duilio, Simeto) in a climate change context, weather and agronomic datasets over the period 1973–2004 from two sites, Benatzu and Ussana (Southern Sardinia, Itay), were used and the model responses were interpreted considering the role of DREB genes in the genotype performance with a focus on drought conditions. The CERES-Wheat crop model was calibrated and validated for grain yield, earliness and kernel weight. Forty-eight synthetic scenarios were used: 6 scenarios with increasing maximum air temperature; 6 scenarios with decreasing rainfall; 36 scenarios combining increasing temperature and decreasing rainfall. The simulated effects on yields, anthesis and kernel weights resulted in yield reduction, increasing kernel weight, and shortened growth duration in both sites. Creso (late cultivar) was the most sensitive to simulated climate conditions. Simeto and Duilio (early cultivars) showed lower simulated yield reductions and a larger anticipation of anthesis date. Observed data showed the same responses for the three cultivars in both sites. The CERES-Wheat model proved to be effective in representing reality and can be used in crop breeding programs with a molecular approach aiming at developing molecular markers for the resistance to drought stress.

Life Cycle Assessment (the systematic analysis of the environmental impact of products during their entire life cycle), Carbon Footprint and Water Footprint assessments play an important role in decision-making processes. These assessments can help guide land management decisions and will likely play a larger role in the future, especially in natural areas with high biodiversity. Agriculture is a substantial consumer of fresh water, so it is important to identify causes and possible solutions to optimize agricultural water use. Water footprint assessments consider water consumption from several points of view and aid in reaching Sustainable Development Goals. Olive trees are a widespread agricultural crop growing in the Mediterranean Basin and are particularly important in the Umbria region in Italy. This paper estimates the water footprint impact related to the production of 1 kg of olives in a rainfed olive orchard managed using low environmental impact techniques. Eleven years of data collection (meteorological data, olives yield data, processes data) are analyzed for typical rural conditions. The results show that local management techniques have lower water requirements than standard international usages. These results can be used to improve and to further explore agricultural water use.

AbstractHerbicides inhibiting either aromatic or branched-chain amino acid biosynthesis trigger similar physiological responses in plants, despite their different mechanism of action. Both types of herbicides are known to activate ethanol fermentation by inducing the expression of fermentative genes; however, the mechanism of such transcriptional regulation has not been investigated so far. In plants exposed to low-oxygen conditions, ethanol fermentation is transcriptionally controlled by the ethylene response factors-VII (ERF-VIIs), whose stability is controlled in an oxygen-dependent manner by the Cys-Arg branch of the N-degron pathway. In this study, we investigated the role of ERF-VIIs in the regulation of the ethanol fermentation pathway in herbicide-treated Arabidopsis plants grown under aerobic conditions. Our results demonstrate that these transcriptional regulators are stabilized in response to herbicide treatment and are required for ethanol fermentation in these conditions. We also observed that mutants with reduced fermentative potential exhibit higher sensitivity to herbicide treatments, thus revealing the existence of a mechanism that mimics oxygen deprivation to activate metabolic pathways that enhance herbicide tolerance. We speculate that this signaling pathway may represent a potential target in agriculture to affect tolerance to herbicides that inhibit amino acid biosynthesis.

AbstractVineyard pruning is a potential lignocellulosic feedstock for bioethanol production from agricultural woody residues, due to its high sugar content and ready availability in whole Europe. Ethanol production from lignocellulosic biomass requires a pretreatment step and then enzymatic hydrolysis process to release sugars for fermentation to ethanol. In this work, alkaline pretreatment with NaOH on vineyard residues was investigated on laboratory scale. The raw material was firstly characterized in order to determine cellulose, hemicellulose and lignin content, using the standard laboratory analytical procedures for biomass analysis provided by the National Renewable Energy Laboratory (NREL). Then, based on Response Surface Methodology tool (RSM), a Box-Behnken model was chosen to define a design of experiments (DoE) in terms of the three independent variables that influence the process: the NaOH concentration, the reaction time and the temperature of the pretreatment process. According to the design, 15 samples of raw material were submitted to alkaline pretreatment and subsequent enzymatic hydrolysis and the glucose yield from whole process was calculated. The statistical optimization was carried out with Minitab 17 software, in order to determine the best operative pretreatment condition by maximizing the glucose yield from enzymatic hydrolysis.Results show that the best glucose yield was obtained at the highest sodium hydroxide concentration and temperature of the reaction; this condition allowed to achieve very significant glucose yield thanks to lignin removal performed with the pretreatment.

La production animale est une source essentielle de revenus et d'émissions de gaz à effet de serre (GES) agricoles en Colombie, au Brésil, en Argentine, au Costa Rica, en Uruguay, au Mexique et au Pérou. Plusieurs options de gestion et technologiques, avec un potentiel d'atténuation du méthane entérique, ont été évaluées et leur mise à l'échelle devrait contribuer à la réalisation des objectifs de réduction des émissions de GES. Pourtant, l'adoption généralisée d'options d'atténuation prometteuses reste limitée, ce qui soulève des questions quant à savoir si les objectifs de réduction des émissions envisagés sont réalisables. À l'aide de données générées localement, nous explorons les potentiels d'atténuation des technologies et des pratiques de gestion actuellement proposées pour atténuer les émissions de méthane entérique, pour les systèmes de production bovine dans les pays d'Amérique latine les plus émetteurs. Nous discutons ensuite des obstacles à l'adoption d'innovations qui réduisent considérablement les émissions de méthane entérique d'origine bovine et des changements majeurs dans les politiques et les pratiques qui sont nécessaires pour relever les ambitions nationales dans les pays à forte émission. En utilisant la science la plus récente et la pensée actuelle, nous fournissons notre point de vue sur une approche inclusive et ré-imaginons comment les secteurs universitaire, de la recherche, des affaires et des politiques publiques peuvent soutenir et encourager les changements nécessaires pour élever le niveau d'ambition et atteindre les objectifs de développement durable en envisageant des actions allant de la ferme à l'échelle nationale. La producción ganadera es una fuente fundamental de ingresos y emisiones de gases de efecto invernadero (GEI) agrícolas en Colombia, Brasil, Argentina, Costa Rica, Uruguay, México y Perú. Se han evaluado varias opciones de gestión y tecnológicas, con potencial de mitigación de metano entérico, y se prevé que su escalado contribuya al logro de los objetivos de reducción de emisiones de GEI. Sin embargo, la adopción generalizada de opciones de mitigación prometedoras sigue siendo limitada, lo que plantea dudas sobre si los objetivos de reducción de emisiones previstos son alcanzables. Utilizando datos generados localmente, exploramos los potenciales de mitigación de las tecnologías y prácticas de manejo actualmente propuestas para mitigar las emisiones de metano entérico, para los sistemas de producción ganadera en los países de mayor emisión de América Latina. Luego discutimos las barreras para adoptar innovaciones que reduzcan significativamente las emisiones de metano entérico en el ganado y los cambios importantes en las políticas y prácticas que se necesitan para aumentar las ambiciones nacionales en los países con altas emisiones. Utilizando la ciencia más reciente y el pensamiento actual, brindamos nuestra perspectiva sobre un enfoque inclusivo y reimaginamos cómo los sectores académico, de investigación, empresarial y de políticas públicas pueden apoyar e incentivar los cambios necesarios para elevar el nivel de ambición y alcanzar los objetivos de desarrollo sostenible considerando acciones desde la granja hasta la escala nacional. Livestock production is a pivotal source of income and agricultural greenhouse gas (GHG) emissions in Colombia, Brazil, Argentina, Costa Rica, Uruguay, Mexico and Peru. Several management and technological options, with enteric methane mitigation potential, have been evaluated and their scaling is anticipated to contribute towards achieving GHG emission reduction targets. Yet, widespread adoption of promising mitigation options remains limited, raising questions as to whether envisaged emission reduction targets are achievable. Using locally generated data, we explore the mitigation potentials of technologies and management practices currently proposed to mitigate enteric methane emissions, for cattle production systems in the higher emitting countries of Latin America. We then discuss barriers for adopting innovations that significantly reduce cattle-based enteric methane emissions and the major shifts in policy and practice that are needed to raise national ambitions in the high emitting countries. Using the latest science and current thinking, we provide our perspective on an inclusive approach and re-imagine how the academic, research, business and public policy sectors can support and incentivize the changes needed to raise the level of ambition and achieve sustainable development goals considering actions all the way from the farm to the national scale. الإنتاج الحيواني هو مصدر محوري للدخل وانبعاثات غازات الدفيئة الزراعية في كولومبيا والبرازيل والأرجنتين وكوستاريكا وأوروغواي والمكسيك وبيرو. تم تقييم العديد من خيارات الإدارة والخيارات التكنولوجية، مع إمكانية تخفيف الميثان المعوي، ومن المتوقع أن يساهم قياسها في تحقيق أهداف خفض انبعاثات غازات الدفيئة. ومع ذلك، لا يزال الاعتماد الواسع النطاق لخيارات التخفيف الواعدة محدودًا، مما يثير تساؤلات حول ما إذا كانت أهداف خفض الانبعاثات المتوخاة قابلة للتحقيق. باستخدام البيانات التي تم إنشاؤها محليًا، نستكشف إمكانات التخفيف من التقنيات وممارسات الإدارة المقترحة حاليًا للتخفيف من انبعاثات الميثان المعوية، لأنظمة إنتاج الماشية في البلدان ذات الانبعاثات الأعلى في أمريكا اللاتينية. ثم نناقش العوائق التي تحول دون اعتماد الابتكارات التي تقلل بشكل كبير من انبعاثات الميثان المعوي القائم على الماشية والتحولات الرئيسية في السياسات والممارسات اللازمة لرفع الطموحات الوطنية في البلدان ذات الانبعاثات العالية. باستخدام أحدث العلوم والتفكير الحالي، نقدم وجهة نظرنا حول نهج شامل ونعيد تصور كيف يمكن للقطاعات الأكاديمية والبحثية وقطاع الأعمال والسياسة العامة دعم وتحفيز التغييرات اللازمة لرفع مستوى الطموح وتحقيق أهداف التنمية المستدامة مع الأخذ في الاعتبار الإجراءات على طول الطريق من المزرعة إلى النطاق الوطني.